龙归酒毒理学安全性评价

目的了解龙归酒的饮用安全性。方法采用小鼠急性经口毒性试验、小鼠骨髓微核试验、小鼠精子畸形试验、Ames试验和30d喂养试验进行检测。结果龙归酒小鼠经口MTD〉20．0g／kg体重，属无毒级；小鼠骨谴微核试验、小鼠精于畸形试验和Ames试验结果均为阴性，无遗传毒性；30d喂养试验受斌物各剂量组大鼠体重、食物利用率、血液学和血液生化学指标、脏体比与对照组比较差别无显著性（P〉0．05），主要脏器在外观形态和病理组织学上均未见异常变化，未显示明显毒性作用。结论龙归酒可安全饮用。     

不同宿主来源的华支睾吸虫同工酶比较观察

目的:通过对不同宿主华支睾吸虫的同工酶研究,了解其在不同宿主中的变化情况.方法:应用浓度梯度聚丙烯酰胺凝胶板状电泳(CG-PAGE)对50 d虫体的乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)、酯酶(EST)、过氧化物酶(PO)进行电泳观察,通过凝胶成像软件对酶(蛋白)带的迁移率、相对浓度分布、光密度等结果进行分析.结果:来自不同宿主(大鼠、犬、豚鼠、兔、猫)的虫体主要酶带特征基本相同,但次要酶带存在一定差异.结论:不同宿主的华支睾吸虫同工酶存在着一定的变化,实验结果为华支睾吸虫在不同宿主中的发育、生理、生化提供实验资料.     

Radical Scavenging Evaluation of Green Tea Extracts

The free radical scavenging activity of Thea sinensis leaf extract was evaluated with a chemical test involving diphenylpicrylhydrazyl radical (DPPH). The hot aqueous green tea extract demonstrated antiradical activity comparable with rutin and vitamin E used as a standard. Liquid–liquid fractionation monitored by scavenging radical bioassay followed by preparative high pressure liquid chromatography led to catechin derivatives. The antiradical activity of the main component of tea leaves was identified as epigallocatechin gallate the major component demonstrating activity.     

当归补血汤含药血清体外诱导耳蜗干细胞的增殖分化

目的：观察当归补血汤对耳蜗干细胞体外诱导分化的影响,并探讨浓度对其的影响。方法：分离新生大鼠Corti器的耳蜗干细胞进行细胞培养,在相差显微镜下观察细胞形态及生长状况,用Nestin免疫荧光法进行耳蜗干细胞鉴定。采用不同剂量当归补血汤进行诱导分化后,采用MyosinⅦA、P27~(KIP1)免疫荧光方法,鉴定内耳毛细胞和支持细胞,并进行细胞计数。结果：当归补血汤细胞诱导分化的内耳毛细胞和内耳支持细胞数量明显较对照组多,其中剂量为0．5 g/ml组向内耳毛细胞的分化比例最高。结论：当归补血汤可以提高耳蜗干细胞体外分化为内耳毛细胞和支持细胞的数量。其中剂量0．5 g/ml组向内耳毛细胞的分化数量较高。     

当归的等级化研究

中草药标准化是非常有必要的,需要用物理和化学的方法来建立标准.标准化的分级是每位草药生产的指标之-.中国当归的等级分为5级:大体、形状、颜色、香味及杂质是否存在.而韩国的生当归分为三级,分别是60 g以上,20～60 g之间,20 g以下.当归身的重量约占整个当归的30%,其表面颜色是浅黄棕色到褐色.-般没有次品.若有次品,多为腐烂、中空、表面色黑或中节呈褐色.提出了当归标准化的等级,并期待着进-步研究.此研究可以做为中药标准化研究的参考指标.     

Expression of cyclooxygenase-2 in cholangiocarcinoma: correlation with clinicopathological features and prognosis

BACKGROUND AND AIMS: No information is available on the nature of the correlation between cyclooxygenase-2 (COX-2) expression and the clinicopathological features and prognosis of cholangiocarcinoma (CC). The goal of the present study was to determine the possible roles and clinical significance of COX-2 expression in CC. METHODS: We investigated the immunohistochemical expression of COX-2 in 102 patients with CC with respect to clinicopathological characteristics, namely evidence of Clonorchis sinensis infection, proliferation index (PI, assessed by Ki-67 expression), apoptotic index (AI, assessed by TUNEL stain), and microvessel density (MVD, assessed by CD34 expression). Evidence of C. sinensis infection was assessed by the microscopic examination of stools for C. sinensis ova, serological testing (ELISA), and the detection of peripheral bile duct dilations by imaging studies. RESULTS: An immunohistochemical investigation demonstrated the immunolabeling of tumor cells, mainly in the cytoplasmic and perinuclear regions, in 53 (52%) of the 102 patients with CC. No significant differences were found in terms of age, sex, tumor differentiation, involvement of the resection margin, presence of lymph nodes or liver metastases, or in pTNM stage between COX-2 positive and COX-2 negative patients. However, evidence of C. sinensis infection was more common in COX-2 positive patients (P < 0.05). No significant differences were found for PI, AI, MVD, or cumulative survival between COX-2 positive and COX-2 negative patients. CONCLUSION: Clonorchis sinensis infection is related to aberrant COX-2 expression in patients with CC. However, COX-2 expression is not related to clinical outcome in CC patients.     

冬虫夏草对肾性高血压大鼠心血管功能的影响

目的观察青海产冬虫夏草对肾性高血压大鼠血压、心肌肥大、心血管功能的影响.方法将体重160 g～180 g健康雄性Wistar大鼠随机分为3组:对照组、高血压纽及治疗组.于术后第10天,大鼠灌胃冬虫夏草煎剂2 mL,每天2次连续20 d,观察冬虫夏草对大鼠血压、心肌肥大、心血管功能的影响.结果冬虫夏草明显降低肾性高血压大鼠血压,改善高血压大鼠的心肌肥大及血管重构.结论冬虫夏草对肾性高血压有治疗作用.     

冬虫夏草和中国拟青霉对小鼠脾细胞T细胞亚型和NK细胞活性的影响

经流氏细胞仪分析,冬虫夏草和中国拟青霉水提液可显增加ConA刺激 的小鼠脾细胞L3T4^ 和Lyt2^ T细胞的增殖,增加脾细胞L3T4^ 和Lyt2^＋T细胞的数,L3T4^ /Lyt2^ T 比值降低。用MTT还原法观察,二均可增加小鼠脾细胞NK细胞的活性。此外,冬虫夏草和中国拟青霉均可增加脾重、吞噬细胞数及吞噬细胞百分率。     

当归补血汤对大鼠局灶性脑缺血再灌注后大脑皮质神经元的保护作用

目的探讨大鼠局灶性脑缺血再灌注后当归补血汤对大脑皮质神经元的保护作用。方法雄性SD大鼠48只随机分为假手术组、大脑中动脉阻塞再灌注组、生理盐水对照组、当归补血汤治疗组。治疗组术前连续6d灌胃,每日2次(早、晚各1次),术后4h再灌胃2次,每次2.5ml,次日取材。观察各组大鼠神经行为学缺陷;脑梗死体积和梗死率变化;血脑屏障通透性变化;大脑皮质神经元的形态和数量;大脑皮质神经元p53和天冬氨酸特异性半胱氨酸蛋白酶3(Caspase-3)的表达情况以及大脑皮质凋亡神经元的形态和数量。结果与缺血再灌注组、生理盐水对照组比较,当归补血汤治疗组2,3,5-氯化三苯四氮唑(TTC)染色显示大脑皮质梗死区体积减少(P0.05);荧光显微镜观察脑组织中伊文斯蓝的含量减少(P0.05),Nissl染色显示大脑皮质神经元数量增加(P0.05);免疫组织化学方法显示大脑皮质神经元Caspase-3、p53的阳性细胞数减少(P0.05);TUNEL染色法显示大脑皮质神经元凋亡细胞减少(P0.05)。结论当归补血汤治疗组能减少大脑皮质神经元的死亡,这与其减少大脑皮质神经元Caspase-3、p53的表达,并通过减少细胞凋亡起保护作用有关。