Effects of Aloe vera on colonic anastomoses of rats

Background: Aloe vera is a cactus‐like plant that grows in hot, dry regions. Aloe vera has been known and used in traditional medicine and also in the modern world. Aloe vera has some pharmacological actions including wound healing, anti‐inflammatory and immunostimulatory effects. We have studied the possible effects of Aloe vera gel on the healing of colonic anastomoses of rats. Material and Methods: In this study, Sprague–Dawley rats were used. Aloe vera group received 2 mL/kg per day Aloe vera, and the control group received the same amount of water. Animals underwent resection and anastomosis of the distal colon. Bursting pressures of anastomoses and hydroxyproline contents of perianastomotic region were determined on the third and seventh days. Mann–Whitney U‐test was used to compare bursting pressures and hydroxyproline levels between groups. Results: There was no difference between groups in mortality. Mean bursting pressures and mean hydroxyproline levels were lower in the control group than in the Aloe vera group both on the third and seventh days. Conclusion: Aloe vera has multiple pharmacologically active compounds. It stimulates phagocyte formation and its activity induces nitric oxide production, it has angiogenic activity, increases synthesis, maturation and cross‐linking of collagen, stimulates cell proliferation, stimulates fibroblast functions and proliferation, inhibits arachidonic acid oxidation, has anti‐inflammatory effects, reduces tumour necrosis factor‐α levels, and has antioxidant activity. Thus, according to our findings, Aloe vera has positive effects on the colonic anastomotic healing of rats.     

鲜芦荟汁对血小板减少患者穿刺针眼止血效果观察

目的观察鲜芦荟汁对血小板减少患者穿刺针眼止血的效果。方法将38例患者按住院时间顺序分为两组:对照组12例采用经典的压迫止血法止血65例次,观察组26例采用鲜芦荟汁压迫止血140例次,观察两组止血所需时间及不良反应。结果两种方法的止血效果间差别有统计学意义(Uc=11·309,P<0·0005);观察组患者在后期均未发生淤血、血肿、过敏等不良反应,而对照组中有3例发生不良反应,两组间差别有统计学意义(χ2=6·558,P<0·05)。结论运用鲜芦荟汁对血小板减少患者针眼处进行止血效果较好。     

One-year chronic toxicity study of Aloe arborescens Miller var. natalensis Berger in Wistar Hannover rats. A pilot study.

This study was conducted to evaluate the chronic toxicity of Aloe arborescens Miller var. natalensis Berger (ALOE) in the diet at doses of 4.0%, 0.8% or 0.16% to groups of male and female Wistar Hannover rats. No deaths occurred at any dose level throughout the treatment period. Both sexes receiving 4.0% showed diarrhea, with a reduced body weight gain. Increase of WBCs in the male 4.0% group, decrease of Hb in the female 4.0% and 0.8% groups, decrease of IP in the male 4.0% and 0.8% groups and female 4.0% group, and decrease of Ca and ALT in the female 4.0% group were observed. Relative kidney weight showed increase in the female 4.0% group and relative heart and brain weights were decreased in the female 4.0% and 0.8% groups. Histopathologically, both sexes receiving 4.0% showed severe sinus dilatation of ileocecal lymph nodes, and yellowish pigmentation of ileocecal lymph nodes and renal tubules. In conclusion, the no observed adverse effect level (NOAEL) for ALOE was the 0.16% in diet, which is equivalent to 87.7 and 109.7 mg/kg/day in males and females, respectively.     

聚酰胺树脂分离纯化芦荟蒽醌衍生物

目的 研究纯化芦荟蒽醌衍生物的工艺条件.方法 对照乙醇梯度洗脱所得各组分的HPLC谱图,考察聚酰胺树脂富集、纯化芦荟蒽醌的吸附性能和洗脱参数.结果 通过聚酰胺树脂富集与纯化,并采用40%,60%,70%,80%乙醇梯度洗脱,可以使芦荟蒽醌衍生物得到较好的分离.结论 该法的精确度、稳定性、重复性和回收率都较好,且操作简便易行.     

芦荟浓缩液治疗放疗性皮肤急性损伤的临床观察

目的　观察芦荟浓缩液治疗放疗性皮肤急性损伤的临床效果。　方法　 2 2 4例放疗性皮肤急性损伤患者随机平均分为 2组 ,治疗组采用芦荟浓缩液涂擦患处 ,对照组采用氟哌酸涂擦患处 ,比较两组的治愈率。　结果　治疗组治愈率为 94 6% ,对照组治愈率为 75 9% ,两组治愈率差异有显著性 (P <0 0 5 )。　结论　芦荟浓缩液治疗皮肤急性放射损伤疗程短 ,应用过程中患者感觉清凉舒适 ,容易接受 ,在临床上值得推广应用     

芦荟多糖对体外培养表皮细胞增殖活性及细胞内钙水平的影响

目的:研究芦荟多糖对体外培养人表皮细胞增殖活性及细胞内钙离子水平的影响。方法:在表皮细胞培养液中加入不同浓度芦荟多糖(25,50,100,200,400mg.L-1),应用细胞计数、四甲基偶氮唑蓝(MTT)法观察细胞增殖情况,采用Fura-2钙荧光指示剂测定细胞内钙离子浓度。结果:芦荟多糖作用2d后,100mg.L-1以上芦荟多糖组表皮细胞增殖明显增快(P<0.01),并呈剂量依赖性;与对照组比较,200mg.L-1和400mg.L-1芦荟多糖组表皮细胞内钙离子浓度显著性升高(P<0.05)。结论:芦荟多糖可能是通过调节细胞内钙离子浓度发挥其促进增殖作用。     

芦荟大黄素体外抑制人舌癌及肺癌肿瘤细胞生长的初步研究

目的观察芦荟大黄素在体外对人舌癌细胞株Tca 8113及肺癌细胞株YTMLC肿瘤细胞的生长抑制作用．方法不同浓度的药物作用于培养的肿瘤细胞，应用MTT法和光学显微镜观察药物对细胞的生长抑制作用．结果随着药物作用浓度升高，细胞增殖随之下降，且该抑制作用具有一定的浓度-时间-效应关系，在芦荟大黄素浓度为10mg／L时抑制作用最为显著．结论芦荟大黄素在体外可以对人舌癌细胞株Tca 8113和肺癌细胞株YTMLC肿瘤细胞产生生长抑制作用．     

木立芦荟中BACE抑制活性成分的研究

目的从木立芦荟(Aloe arborescens)中寻找新的BACE(β-分泌酶)抑制活性成分。方法用硅胶正相色谱及反相色谱技术进行活性成分的分离纯化，根据理化性质、光谱资料进行结构鉴定。结果从木立芦荟95%乙醇提取物的乙酸乙酯部分和正丁醇部分，分离得到了8个化合物：异丁酰基芦荟宁A(1)，芦荟宁A(2)，芦荟大黄素(3)，(E)-2-acetonyl-8-(2′-O-feruloxyl)-β-D-glucopyranosyl-7-methoxy-5-methyl-chromone (4)，7-O-methyl aloeresin A (5)，芦荟苷A(6)，elgonica-dimer A (7)和elgonica-dimer B (8)。结论化合物1为新化合物，化合物4为首次从该植物中分离得到。活性测试结果表明，化合物2，4，5，6对BACE有一定的抑制活性。     

芦荟汁对小鼠腹部伤口愈合情况影响的研究

目的：本课题主要研究芦荟汁对小鼠腹部伤口愈合情况影响。方法选择40只成年小鼠为研究对象并随机分为2组。分别采用0.9%生理盐水和芦荟鲜汁对两组进行涂擦小鼠伤口,观察小鼠在给药后一周伤口愈合情况。结果与对照组比较,实验组小鼠的伤口愈合情况明显好转：有效率比较（P〈0.05）。结论芦荟汁对小鼠腹部伤口愈合具有促进作用。     

乙醇、芦荟联合龙血竭外敷治疗Ⅱ期压疮的护理

目的探讨外用乙醇、芦荟联合龙血竭治疗Ⅱ期压疮的疗效。方法将Ⅱ期压疮患者38例随机分成观察组和对照组,各19例。在予以全身综合治疗及常规压疮护理基础上,观察组采用乙醇、芦荟联合龙血竭外敷创面。结果观察组与对照组2周内治疗有效率分别为947％和842％,差别无统计学意义（χ2=1118;P〉0．05）,但观察组的治愈率显著高于对照组,分别为84．2％和47．4％,差别有统计学意义（χ2=5．729;P〈0.05）。结论乙醇、芦荟联合龙血竭外敷对促进Ⅱ期压疮愈合具有良好的治疗效果。