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131.
本文报告口服Sumatriptan 100mg对偏头痛急性发作119例次的治疗结果。治疗后4h内显效91例次(76.5%),好转16例次(13.4%),无效12例次(10.1%),总有效率为89.9%。对偏头痛伴随症状恶心、呕吐和畏光、畏声的缓解率分别为94.2%、96%和94.3%。 相似文献
132.
Geraldo F. Busatto Lyn S. Pilowsky Durval C. Costa John Mertehs Dirk Terriere Peter J. Ell Rachel Mulligan Michael J. Travis Josée E. Leysen Dominic Lui Sveto Gacinovic Wendy Waddington Anne Lingford-Hughes Robert W. Kerwin 《European journal of nuclear medicine and molecular imaging》1997,24(2):119-124
The mapping of 5-HT2 receptors in the brain using functional imaging techniques has been limited by a relative lack of selective radioligands.
Iodine-123 labelled 4-amino-N-[1-[3-(4-fluorophenoxy)propyl]-4-methyl-4-piperidinyl]-5-iodo-2-methoxybenzamide (123I-5-I-R91150 or123I-R93274) is a new ligand for single-photon emission tomography (SPET), with high affinity and selectivity for 5-HT2A receptors. This study reports on preliminary123I-5-I-R91150 SPET, wholebody and blood distribution findings in five healthy human volunteers. Maximal brain uptake was approximately
2% of total body counts at 180 min post injection (p.i.). Dynamic SPET sequences were acquired with the brain-dedicated, single-slice
multi-detector system SEM-810 over 200 min p.i. Early peak uptake (at 5 min p.i.) was seen in the cerebellum, a region free
from 5HT2A receptors. In contrast, radioligand binding in the frontal cortex increased steadily over time, up to a peak at approximately
100–120 min p.i. Frontal cortex-cerebellum activity ratios reached values of 1.4, and remained stable from approximately 100
min p.i. onwards. Multi-slice SPET sequences showed a pattern of regional variation of binding compatible with the autoradiographic
data on the distribution of 5-HT2A receptors in (cerebral cortex>striatum>cerebellum). These findings suggest that123I-5-I-R91150 may be used for the imaging of 5-HT2A receptors in the living human brain with SPET. 相似文献
133.
134.
IgM allotype heterozygous F1 mice were independently suppressed for Igh6a or Igh6b to
evaluate the contribution of B-1 and B-2 cells to natural serum IgM levels and Ab responses.
B-2 B cells expressing IgM of the suppressed allotype were evident in the spleens of
suppressed mice 4 to 6 weeks after cessation of the suppression regimen, whereas B-1 B
cells of the suppressed allotype were undetectable for up to 9 months. Although serum IgM
of the suppressed allotype was initially depleted in mice suppressed for either allotype, by
7 months of age, there were detectable levels of IgM of the suppressed allotype in the
serum; however, the levels were significantly below that found in nonsuppressed mice.
When mice were immunized with either the T-independent or T-dependent form of
phosphorylcholine, those suppressed for either allotype, and consequently depleted of B-1
B cells of that allotype, did not respond with phosphorylcholine-specific IgM of the
suppressed allotype. In contrast, when mice were immunized with α1-3 dextran, the Igh6a
allotype-suppressed mice were able to produce dextran-specific IgM of that allotype. These
results show that allotype-bearing B-1 cells of both allotypes can be effectively suppressed
by this suppression protocol and this produces long-lasting effects on B-1 cell levels and
serum IgM of the suppressed allotype. These observations reflect the derivation of the
majority of B-1 cells from fetal-neonatal precursors, which cannot be replaced by newly
emerging B-2 cells of adult origin. Their ablation by antibody treatment results in
permanent alterations to the adult B-cell repertoire. 相似文献
135.
Kaisa Heiskanen Pirjo Lindstr m-Sepp Leena Haataja Sirkka-Liisa Vaittinen Terttu Vartiainen Hannu Komulainen 《Toxicology》1995,100(1-3):121-128
Activities of the xenobiotic metabolizing enzymes were measured in the liver, kidney, duodenum and lung microsomes and cytosol fractions of Wistar rats after subchronic administration of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX), a potent bacterial mutagen in chlorinated drinking water. MX was administered by gavage at the dose level of 30 mg/kg for 18 weeks (low dose), or at the dose level which was raised gradually from 45 mg/kg for 7 weeks via 60 mg/kg for 2 weeks to a clearly toxic dose of 75 mg/kg for 5 weeks (high dose). Microsomal and cytosolic preparations were made and the activities of 7-ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-dealkylase (PROD), NADPH-cytochrome-c-reductase, UDP-glucuronosyltransferase (UDPGT) and glutathione-S-transferase (GST) were measured. Kidneys were affected most. A dose-dependent decrease was observed in EROD (90% in males, 80% in females at the high dose) and in PROD (58% in females, at the high dose) in kidneys. An increase was, however, detected in kidney NADPH-cytochrome-c-reductase (66% in females at high dose), UDPGT (89% in males and 97% in females at high dose) and GST activities (56% in males and 50% in females at high dose). MX caused only a few changes in the enzyme activities of the liver. The EROD activity was decreased 25% to 37%, both in the livers of males and females, but the total content of P450s was not altered. Hepatic GST activity was elevated in females in a dose-dependent manner (31% and 44%). GST activity was elevated in duodenum in females (59%) at the high dose. There were no marked changes in the enzyme activities in the lungs. MX was a weak inhibitor of EROD activity both in the liver and kidney microsomes in vitro, decreasing the EROD activity by 53% and 43%, respectively at the concentration of 0.9 mM. The results indicate that MX decreases the activity of phase I metabolism enzymes, but induces phase II conjugation enzyme activities, particularly in kidneys in vivo. It is possible that these changes contribute to metabolism of MX in kidneys and renders them susceptible to MX in the course of repeated exposure. 相似文献
136.
气功对冠心病人CM_5ST段/心率斜率的影响 总被引:1,自引:0,他引:1
37名冠心病患者随机分为气功治疗组(22人)和对照组(15人)。气功治疗组接受12周动功治疗,对照组接受安慰剂治疗,治疗前后均测定CM_5 ST段/心率斜率。结果发现动功治疗组CM_5 ST段/心率斜率减少,而对照组不变或增大,两组有显著性差异,ST段下移及常规分级运动试验其它指标在两组间无显著差异。表明CM_5ST段/心率斜率是一个敏感的心肌缺血指标。 相似文献
137.
138.
小檗碱对大鼠阴茎海绵体磷酸二酯酶5mRNA水平的影响 总被引:3,自引:0,他引:3
目的 :检测大鼠阴茎海绵体中磷酸二酯酶 5 (PDE5 )mRNA的表达 ,进而探讨小檗碱 (berberine ,Ber)的分子作用机制。 方法 :通过逆转录酶链反应 (RT PCR)技术检测大鼠阴茎海绵体中PDE5mRNA的表达。 结果 :大鼠阴茎海绵体中有PDE5A1和PDE5A2mRNA的表达 ,以PDE5A2为主要的异构酶。与内参照 β 肌动蛋白相比 ,对照组、Ber孵育 1和 3h组的PDE5A1及PDE5A2基因mRNA的相对表达量分别为 :0 .2 2± 0 .0 2 ,0 .4 1± 0 .0 1 ;0 .1 5± 0 .0 1 ,0 .34± 0 .0 2 ;0 .1 0± 0 .0 1 ,0 .1 2± 0 .0 1。与对照组相比 ,PDE5A1、PDE5A2的mRNA表达 ,在Ber孵育 1h组降低了 32 %和 1 7%,3h组降低了 5 5 %和 71 %,其中尤以应用Ber 3h后PDE5A2的mRNA减少最为明显 (n =5 ,P <0 .0 1 )。 结论 :Ber对NO cGMP信号通路的下游关键酶 (PDE5 )具有一定的调控作用 ,尤其是抑制PDE5A2的mRNA表达 ,为Ber治疗勃起功能障碍的分子机制之一。 相似文献
139.
糖尿病雄性大鼠性腺5α-还原酶Ⅱ型活性变化 总被引:5,自引:0,他引:5
目的 :探讨大鼠青春期和成年期糖尿病时性腺 5α 还原酶Ⅱ型的活性变化。 方法 :取 4 0d和 90d龄雄性Wistar大鼠各 30只 ,分别分为对照组 (C)、糖尿病组 (D)和胰岛素治疗糖尿病组 (ID)。采用薄层层析法测定各组附睾头、附睾尾、前列腺和睾丸组织内 5α 还原酶 Ⅱ型的活性。 结果 :①青春期大鼠性腺的各部位内 ,5α 还原酶 Ⅱ 型的活性D组均明显低于C组 (P <0 .0 1) ,而ID组明显高于D组 (P <0 .0 1) ;②成年期大鼠性腺的各部位内 ,5α 还原酶 Ⅱ型的活性在C、D和ID组各组间差异无显著性 (P均 >0 .0 5 )。 结论 :青春期大鼠性腺内 5α 还原酶 Ⅱ型的活性更易受代谢环境、激素水平及局部特异性因素的影响 ,而成年期大鼠性腺内 5α 还原酶Ⅱ 型的活性相对稳定 相似文献
140.
Objective: To study the changes in 5α-reductase type Ⅱactivity in sex glands of pubertal and adult male rats with diabetes. Methods: Pubertal (40 days old) and adult (90 days old) male Wistar rats were used, 30 animals in each group. Each group was randomly divided into 3 subgroups: the control (C), the diabetic (D) and the diabetic with insulin replacement (ID). The activity of 5α-reductase type Ⅱ was determined with thin layer chromatography in the epididymis, prostate and testis. Results: (1) In all the sex glands of pubertal rats, the enzyme activity was significantly lower in group D than in groups C and ID. (2) In all the sex glands of adult rats, there was no significant difference in the activity. Conclusion: In male rats, the activity of 5αa-reductase type Ⅱ in sex glands is more vulnerable to diabetes in pubertal rats than in adult rats. 相似文献