联合菌苗抗肿瘤作用的初步探讨

目的 :探讨联合菌苗防治肿瘤的作用。方法采用动物体内实验 ,动物为封闭群KM小鼠 ,菌株为 2 6 0 0 3- 2 1、4 482 4 - 3及 932 0 1- 3,瘤种为S180 。所有实验小鼠接种S180 后 ,按随机对照试验设计分成 5组 ,即三个不同的单价菌苗组、一个联合菌苗组及一个对照组 ,同期观察联合菌苗对S180 增长的影响。结果 :三个不同的单价菌苗组及联合菌苗组的平均瘤重均比对照组低 ,联合菌苗组的平均瘤重比单价菌苗组的更低 ,差异均有高度统计学意义 (P <0 .0 1～ 0 .0 0 1) ;三个不同单价菌苗组的抑瘤率分别为 5 0 .6 %、4 8.3%、4 5 .5 % ,联合菌苗组抑瘤率为 75 .0 %。结论 :实验所选用的菌苗能抑制S180 的增长 ,联合菌苗的效果更好     

病案工作的法律作用

病案是法律上的可靠证据,病案主要是作为病人状况、病史和预后公正的证据,所有的评价此时都没有考虑法庭的作用,因此是非常有价值的。无论是法庭内外即鉴定意外事故受伤的范围;又能证实医务人员或医院在对病人治疗中的疏忽或其它不当行为,它有助于保护病人、医院和医护人员的合法权益。 1.病案在诉讼活动中也处于中心地位 诊治活动主要是围绕证据而开展的,只有在查明案件事实,才谈得上正确适用法律。作为刑事被告人,证据是对其定罪量刑的依据,作为民事诉讼当事人,若举证不当,可能带来败诉的结果。可见,病案对维护公民合法权益和处理民事纠纷至关重要。 2.病历在伤害案件中具有特殊的地位 因为受害人是否遇到伤害,损伤程度的轻重,是加害人是否承担和承担何种法律责任的重要依据。例如,轻微伤害的只     

鳗油精治疗儿童学习困难的临床观察

鳗油精是以活鳗鱼为原料,运用先进技术精炼而成的纯天然药品。它富含人体自身不能合成的必需脂肪酸——二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)。凡含有EPA和DHA的脂肪酸,统称Omega-3(ω-3)脂肪酸,它是一种不饱和脂肪酸,具有较强的调脂作用,这类脂肪酸可促进脑的发育,且可能有助于防止脑老化。为进一步观察鳗油精对小儿的益智健脑作用,我们从1995年2月到5月间,选择儿童“学习困难”专科门诊中10名对象,以自愿原则,用鳗油精进行治疗,并对其临床效果作了观察。     

维生素E在高硫对动物血清硒及过氧化脂质水平影响中的作用

本文能家兔为实验对象，观察维生素E(VE)在高硫(S)对动物血清硒(Se)及脂质过氧化产物水平影响中的作用．结果发现；高S导致动物血清Se降低、血清MDA浓度升高．饲料中添加VE后，血清VE水平显著增加，机体增强了VE的抗氧化作用，因而血清MDA水平显著低于对照组和高S组，表明动物在摄取高S的同时补充VE对增强动物的抗氧化作用是有一定意义的。     

1-乙基-6-氟-1,4-二氢-4-氧代-7-(4-芳酰硫代氨甲酰基-1-哌嗪基)-3-喹啉羧酸的合成及抗菌作用

Thirteen new 1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(4-aroyl-thiocarbamoyl- 1 piperazinyl)-3-quinoline carboxylic acids were prepared, Their structures were characterized by elemental analysis, IR, HNMR and MS spectra.Preliminary pharmacological tests indicated that some of compounds Ia～m possess strong inhibiting activity against Escherichia coli, Bacillus subtilis and Proteus at concentration of 100 μg/ml.     

卫生事业的性质、地位与作用的讨论

卫生事业的性质、地位与作用的讨论彭瑞骢，李东方（北京医科大学１０００８３）世界银行１９９３年报告中指出中国以全球卫生经费的１％较好地解决了全球２２％人口的卫生保健问题，效果是好的。最近第２版牛津公共卫生教科书上介绍了英国、美国、日本及中国的卫生工作是...     

小儿膝关节以下手术后股神经加坐骨神经阻滞镇痛

由于小儿对疼痛耐受力低，合作差，常影响术后康复，且采用麻醉性镇痛药多属于禁忌和相对禁忌。自2003年3月，采用股神经加坐骨神经阻滞镇痛，收到较好效果，现总结如下。     

甲氰咪胍致昏迷1例

甲氰咪胍现临床广泛应用于治疗消化性溃疡，对胃酸分泌、组织胺、促胃液素等的分泌均有强大的抑制作用，对急性胃出血也有较好的止血作用。但有关副作用的报道也逐年增多。现将甲氰咪胍致昏迷1例报告如下。     

地塞米松对布比卡因诱导小鼠神经元毒性的影响

Objective To investigate the effect of dexamethasone on the toxicity of bupivacaine in murine neurons.Methods Murine neuroblastoma cell line N2a was obtained from ATCC cell bank (USA). The cells were cultured in 10% fetal cow serum/MEM culture medium and divided into 4 groups voup I control (Con); group II bupivacaine ( Bup); group Ⅲ dexamethasone (Dex) and group IV Dex + Bup. The culture medium contained bupivacaine 900 μmol/L in group Bup and dexamethasone 1 μmol/L in group Dex respectively. In group Dex + Bup ( IV ) Bup was added to the culture medium with a final concentration of 900 μmol/L at 12 h after pretreatment with Dex 1 μmol/L. The cells were inoculated in 24 well plates (0.5 ml in each well, 24 wells in each group) and 10 cm culture dishes (7 ml in each dish, 4 dishes in each group). The release rate of LDH was calculated and the morphology of the cells and nucleus condensation (by Hoechst 3334224 fluorescent staining) was detected at 9 h of incubation in 24 well plates. The mitochondrial transmembrane potential (by JC-1 assay) and phosphorylation of Akt and ERKs (by Western blot) were measured at 5 h of incubation in 24 well plates and in culture dishes respectively. ResultsBupivacaine caused severe damage to the N2a cells as evidenced by increase in LDH release and nucleus condensation (apoptosis), dephosphorylation of Akt and ERKs, decrease in mitochondrial transmembrane potential and severe morphological changes. Dexamethasone pretreatment significantly attenuated bupivacaine-induced neurotoxicity. Conclusion Dexamethasone can protect N2a cells from bupivacaine-induced neurotoxicity through stabilization of mitochondrial transmembrane potential and inhibition of dephosphorylation of Akt and ERKs.