深部毛霉菌感染4例

例1,女,47岁,因急性混合细胞型白血病接受VDAP(VCR,DNA,Ara C,Pred)方案化疗后,外周血WBC<1.0×109/L,持续了18d,化疗后6d患者发热,双肺可闻湿性口罗音,至化疗后21d患者右肺大片致密影,经多种抗生素治疗无效。经纤维支气管镜检查发现,沿支气管壁见大量白色串珠样膜样分泌物。支气管灌洗液、尿、痰培养均为毛霉菌生长。尽管用二性霉素B治疗,患者在化疗后25d死于多脏器功能衰竭、弥漫性血管内凝血、感染性休克。例2,男,26岁,因慢性粒细胞白血病4月余作非血缘异基因造血干细胞移植,移植后用环孢素A加骁悉预防和治疗GVHD。移植后137d…     

血小板活化因子促进大鼠气道平滑肌细胞增殖及其作用机制

目的研究血小板活化因子（PAF）对大鼠气道平滑肌细胞（ASMC）增殖活性的影响，并探讨其作用机制。方法以10^-7mol／LPAF作为刺激因素，以20mmol／LN-乙酰半胱氨酸（NAC）进行干预。用四唑盐（MTT）比色法检测细胞增殖活力；流式细胞仪测定细胞周期；免疫细胞化学染色（SABC法）检测细胞核增殖抗原（PCNA）的表达；EMSA法检测ASMC中NF-κB的活性。结果发现PAF能显著促进ASMC的增殖，明显增加细胞增殖指数及其PCNA阳性表达率，并使ASMC细胞核内NF-κB活性明显增加。预先用NAC干预可显著缓解上述变化（P〈0．05）。结论PAF能促进ASMC的增殖，这一调控部分是通过激活NF-κB通路而发挥作用。提示PAF是气道重建的重要刺激因子。     

Effect of nuclear factor-κB p65 ASOND on I typ collagen expression and rat hepatic stellate cells proliferation

Objective Sstudy effect of nuclear factor-κB ASOND on I type collagen expression and rat hepatic stellate cells(HSC)proliferation.Methods Rat HSCs were separated by affusing and digestingof Ⅳ type collagenenzyme and density acentric method.Lipid-mediated NF-κB p65 ASOND(0.001,0.01,0.1,1μmol/L)Was transferred into rat HSCs.Toxicity of HSCs caused by NF-κB p65 ASOND and activity of LDH were determined by trypan blue staining.Proliferation affection of transferring NF-κB p65 ASOND into HSCs was determined by MTT.In different concentration NF-κB p65 ASOND.expression of Ⅰ type collagen stimulated by 1mg/L TNF-αwas determined by RT-PCR and ELISA.Results After transfection of NF-κB p65 ASODN,expression of NF-κB protein in HSCs was decreasing.Toxicity experiment indicated that NF-κB p65 ASOND of different concentration(0.001,0.01,0.1 and 1.0 μmol/L)had no effect on HSCs livability and LDH activity(P<0.05).Four different concentration NF-κ B p65 ASOND could restrain HSCs proliferation stimulated by 1 mg/L TNF-α.The expression of I type collagen and mRNA stimulated by 1mg/LTNF-αwas increased,and had a positive correlation with concentration(P>0.05).Conclusion NF-κB p65 ASOND may depress NF-κB activity to restrain HSCs proliferation and Ⅰ type collagen expression,and reduce extracellular matrix.     

应用超声胎盘分级对妊高症监护的进一步观察(附123例报道)

由于妊高症原因不明,但对母子的威胁大,孕产妇的并发症多,围产儿死亡率高,因此迫切需要一种简便易行、对母子无损的监护方法。胎盘是胎儿的器官其成熟过程随胎儿发育成长,理论上讲,胎盘的变化随妊高症的病情有所改变,并应根据胎盘的超微结构、显微镜下的改变以及大体形态学改变、影像学改变,进一步了解疾病对胎儿的影响。本文以B超声胎盘分级对妊高症的监护作用作了进一步观察。     

单采中等量间断放血血浆转换治疗呼吸麻痹型格林—巴利综合征的…

应用单采中等量间断放血血浆置换治疗３０例呼吸麻痹型格林－巴利综合征病人，２０例显效，总有效率达９０５，本疗法对呼吸麻痹型可抢救生命，中度病人可显著综合病程。     

酮康唑与二性霉素B联合治疗角膜真菌病

酮康唑与二性霉素Ｂ联合治疗角膜真菌病河南省濮阳市人民医院眼科孙桂丽，李丽萍，王夏自１９９０年以来本院共诊治角膜真菌病１７例，采用酮康唑与二性霉素Ｂ联合应用，效果满意。１７例中男９例，女８例，其中农民１０例，工人５例，学生２例，年龄１５─６５岁，均为单...     

外伤性肝损伤48例诊治分析

我科自1985年～1997年共收治腹部外伤350例，其中肝损伤48例．占13.8％，现将诊治体会分析如下。