肝癌组织辅助性T细胞-2细胞因子强势分泌研究

目的　探讨人肝癌组织中辅助性T细胞亚群 (Th1/Th2 )细胞因子表达模式。方法　收集我科手术切除肝癌组织标本 15例 ,肝良性疾病或肝硬化门静脉高压手术患者肝组织标本 15例作为对照 ,β 肌动蛋白 (β actin)作为参照 ,采用半定量逆转录 聚合酶联反应 (RT PCR)方法检测人肝癌组织中γ 干扰素 (IFN γ)和白细胞介素 4(IL 4)mRNA的表达。结果　人肝癌组织中IFN γmRNA表达 (相对数 :1.18± 0 .3 1)比对照组肝组织中IFN γmRNA表达弱 (相对数 :1.86±0 .5 0 ) ,而IL 4mRNA表达 (相对数 :1.73± 0 .5 1)比对照组强 (相对数 :0 .94± 0 .41,P <0 .0 5 )。结论　人肝癌组织中Th1类细胞因子表达弱 ,Th2类细胞因子表达强 ,呈现Th2细胞因子表达模式 ,肝癌组织局部微环境处于免疫抑制状态 ,肿瘤细胞易发生免疫逃逸     

重症肌无力的研究进展

重症肌无力是由乙酰胆碱受体抗体介导的，针对神经肌肉接头处突触后膜上乙酰胆碱受体的自身免疫性疾病。本文主要对其发病机制中的抗体(前膜抗体、抗肌联蛋白抗体、RyR抗体)、细胞因子、淋巴细胞亚群的研究进展及神经电生理改变和治疗中的问题作一综述。     

慢性非细菌性前列腺炎/慢性盆腔疼痛综合征患者前列腺液中炎症因子差异表达的临床意义

Objective To investigate the role of inflammatory cytokines in the pathogenesis of chronic non-bacterial prostatitis/chronic pelvic pain syndrome (CAP/CPPS) patients. Methods The 38 cases with CAP/CPPS patients (18 cases of CAP and 20 cases of CPPS) and 20 cases of healthy controls were selected. The differential expressions of 40 kinds of inflammatory cytokines were detec-ted by antibody arrays in prostate fluid. Results The inflammatory cytokines which increased more than 1.5 times expression have been found. There were seven kinds in CAP including monocyte che-moattractant protein (MCP)-1, solution tumor necrosis factor receptor Ⅱ(s TNF R Ⅱ), platelet-de-rived growth faetor-BB (PDGF-BB), interleukin (IL)-β, IL-11、IL-6、MCP-2 and five kinds in CPPS groups including MCP-1、PDGF-BB、MCP-2、s TNF R Ⅱ、It-11 respectively, compared with healthy control group. The cluster analysis results showed that protein expression of Monocyte chemoattrac-tant protein 1 (MCP-1)and platelet-derived growth factor BB (PDGF-BB) were significantly increased in CAP (3.47 and 2.07 times) and CPPS (2.25 and 2.19 times) compared with healthy control group and were the final polymerization of inflammatory cytokines. The protein expression of interleukin 1 β (IL-1 β), MCP-1 and soluble tumor necrosis factor Ⅱ (s TNF R Ⅱ) in CAP group was increased more than 1.85,1.55,1.67 times compared with CPPS group. Conclusions Elevated expression of inflammatory cytokines may play an important role in the course of CAP/CPPS disease. The extent of the inflammatory response of CAP was higher than CPPS. The inflammatory factors of MCP-1 and PDGF-BB could serve as a novel diagnostic marker.     

体外循环与全身炎症反应综合征

体外循环 (cardiopulmonarybypassCPB)能够引起以心血管功能和肺功能改变为特征的全身炎症反应综合征 (systemicinflammatoryresponsesyndromeSIRS)。虽然大多数病人CPB引起的SIRS仅表现为一定程度的器官功能障碍 ,经过病人机体的自身调节、临床干预等措施 ,患者的死亡率很低 ,但如患者伴有严重的肺损伤或合并其它重要器官的严重功能障碍 ,则可导致SIRS发展失控 ,患者的死亡率可明显增高。因此 ,充分认识CPB引起SIRS的病理生理机制对于降低高风险患者的死亡率、保证CPB下的心血管手术更安全具有十分重要的意义。1　CPB引起SIR…     

Thl/Th2型细胞因子与妊高征相关性的研究

目的 :探讨 Thl/ Th2型细胞因子与妊高征的相关关系。方法 :随机抽取 4 2例妊高征孕妇和 30例正常孕妇静脉血 ,采用 RIA、EL ISA法检测其血清中 TNF- α、IL- 10水平。结果 :与正常孕妇相比 ,妊高征患者 TNF- α水平显著升高 ,有统计学意义 (P<0 .0 5 ) ;IL - 10水平下降 ,有显著性差异 (P<0 .0 5 ) ;IL— 10 / TNF-α比值下降 ,差异有显著性 (P<0 .0 1)且 TNF-α水平与收缩压、舒张压、平均动脉压、尿蛋白半定量及水肿和水肿级别呈显著正相关。而 IL - 10和 IL - 10 / TNF-α比值与收缩压、舒张压、平均动脉压及尿蛋白呈显著负相关。结论 :妊高征患者存在 Thl/ Th2型细胞因子网络失衡 ,与病情密切相关     

静注丙种球蛋白治疗RSV毛细支气管炎的疗效观察

1　对象和方法1.1　对象　 2 0 0 2 - 10～ 2 0 0 3 - 0 2我科住院的毛细支气管炎 (简称毛支 )患儿 ,诊断符合《实用儿科学》毛支诊断标准 ,鼻咽分泌物呼吸道合胞病毒 (RSV)检测阳性的毛支患儿 6 0例 ,男 4 0例 ,女 2 0例 ,年龄 4 0日龄～ 18月龄 ,随机分为治疗组 3 0例 ,常规组 3 0例。两组在年龄、性别、病情严重程度方面无显著差异。取正常 2 5例为对照组 ,系年龄、性别相匹配的健康儿童 ,采血前 2周无感染史。1.2　方法　治疗组在常规治疗基础上 ,于入院后 4 8h内加用静注丙种球蛋白 (IVIG) (卫生部成都生物制品所 ) 1g/ (kg· d)静…     

CpG ODN刺激的寻常性银屑病中性粒细胞培养上清液对HaCaT细胞增殖的影响

Objective To investigate the role of nentrophils in the pathogenesis of psoriasis vulgaris. Methods Neutrophils were isolated from venous blood samples of 25 patients with psoriasis vulgaris (including 13 cases of active psoriasis and 12 cases of inactive psoriasis) as well as 25 normal human con-trols, and cultured. Then, these neutrophils were grouped and treated with lipopolysaccharide (LPS, 100 g/L),CpG-A (50 mg/L), CpG-B (50 mg/L), and RPMI 1640 culture medium, respectively, for 24 hours followed by the collection of culture supematants. Human keratinocytes (HaCaT) were cultured in the presence of su-pematants of treated or untreated nentrophils for 72 hours followed by the detection of cell proliferation with MTT assay. To determine the role of proinflammatory factors, SOD/CAT and monoclonal antibody to IL-8 and TNF-alpha of 400 u/mL were used to pretreat HaCaT cells 1 hour prior to the stimulation with super-natants of neutrophils. Results Compared with culture medium, the supematant of unstimulated neutrophils from normal controls or patients with inactive psoriasis had no significant effect on the proliferation of HaCaT cells (P ＞ 0.05), but that from patients with active psoriasis markedly promoted the proliferation of HaCaT cells (t = 2.41, P < 0.05). ARe, stimulation by LPS, CpG-A and CpG-B, the supematant of active patient-derived neutrophils significantly promoted the proliferation of HaCaT cells compared with that of normal control-derived nentrophils (t = 3.11, 2.89, 2.29, respectively, all P < 0.05). In comparison with tmstimulated neutrophils, the supematant from LPS- and CpG-A stimulated nentrophiles significantly accelerated the pro-liferation of HaCaT cells. Furthermore, the proliferation of HaCaT cells induced by the supematants of LPS-,CpG-A-, CpG-B-stimulated neutrophils from psoriatic patients was statistically suppressed by the pretreat-ment with the monoclonal antibody to IL-8, TNF-alpha and SOD/CAT (all P < 0.05). Conclusions In patients with psoriasis vulgaris, there is an abnormal secretion of IL-8, TNF-alpha and superoxide by neutrophils in peripheral blood, and these proinflammatory factors could promote the proliferation of HaCaT cells.     

肝病中的炎性细胞因子调节

大多数急、慢性肝病是以肝内各种前炎和抗炎性细胞因子表达增强的炎性过程为特点的。这些细胞因子是很多炎性肝功能失调的驱动力，常导致肝纤维化和肝硬化。[第一段]     

高热惊厥患儿血清几种细胞因子的测定及意义

【目的】测定高热惊厥(febrile convulsion,FC)时患儿血清几种细胞因子水平变化并探讨其意义。【方法】对82例高热惊厥患儿(FC组)及上感患儿(上感组)40例、正常儿童(正常组)41例分别测定IL-6、IL-8、IL-10和TNF-α水平。【结果】FC患儿血清IL-6、IL-8和TNF-α水平明显高于上感组和正常组,差异有显著性(P<0.001)。【结论】FC发作时患儿血清中IL-6、IL-8和和TNF-α水平明显增高,说明在惊厥发作时有细胞免疫因素的参与。