猪心组织型纤溶酶原激活剂的提纯和鉴定

取猪心组织用丙酮去脂,pH4.2、0.45mol/L醋酸钾缓冲液抽提组织型纤溶酶原激活剂(t-PA)。抽提液经硫酸铵分段盐析,然后用纤维蛋白-Sepharose柱吸附t-PA,2mol/L硫氰酸钾洗脱的t-PA溶液经Sephadex G-150凝胶过滤,得到纯的t-PA。其比活性为39038.5 IU/mg,SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)鉴定其分子量为67000。提纯过程中应用了蛋白酶抑制剂(aprotinin)以及6-氨基己酸,以抑制单链t-PA转变为双链t-PA。t-PA活性测定以尿激酶(UK)为参考标准。     

组织纤溶酶原激活物的分离纯化

用亲和层析法从猪心组织分离纯化纤溶酶原激活物(tissue type plasminogen activator,t-PA),以纤维蛋白-sepharose-4B 和 lys-sepharose-4B 两种亲和胶,从5 kg 新鲜猪心组织中分离出 t-PA1.46mg,比活性为235000 IU/mg,得率为34%,SDS 聚丙烯酰胺凝胶电泳测定为一条带,分子量为68000。     

家猪心脏二尖瓣复合体的形态学观测

目的　观测家猪心脏二尖瓣复合体 ,为家猪心脏研究和心脏移植积累资料。方法　甲醛固定的家猪心脏 3 5例 ,解剖并观测二尖瓣复合体。结果　家猪心脏二尖瓣复合体由瓣环、瓣膜、腱索和乳突肌构成 ,瓣环周长为 73 0 0± 9 71mm ;前瓣、后瓣、前外侧连合、后外侧连合的高度分别为 17 45± 1 99mm、16 3 3±2 2 2mm、5 68± 1 3 7mm、5 3 5± 1 11mm ,前外侧连合、后内侧连合的宽度分别为 4 3 1± 0 80mm、4 40±0 63mm ,前乳头肌起始 ,腱索附着于前瓣、后瓣和后内侧连合的条数为 4 0 0± 1 3 0、5 2 1± 1 84、2 44±1 16,后乳头肌起始 ,腱索附着于前瓣、后瓣和前外侧连合的条数为 3 76± 1 78、5 3 8± 2 2 0、2 12± 0 91。结论　家猪心脏二尖瓣复合体中各结构与人类相似 ,但大小有一定差异     

猪心模型在二尖瓣外科修复教学中的应用

<正>由于二尖瓣修复手术保留了二尖瓣的完整自然结构,能获得较好的心脏功能储备,且能避免人工瓣膜置换相关的近远期不良事件,是瓣膜性疾病最理想的治疗方法。目前国外医疗中心二尖瓣修复术约占二尖瓣手术总量的50%,某些医疗中心甚至明显超过二尖瓣置换术,达到80%以上。而在中国,二尖瓣修复术占二尖瓣手术总量的比例低于5%,即使在心脏外科手术超过千例的医疗中心也不超过10%,这不仅远远低于欧美发     

冷温血停搏液联合灌注对原位猪心移植中的心肌保护作用

目的 研究原位猪心移植中，不同灌注方式的心肌保护效果。方法 将24对家养猪随机分为两组：冷血组(n=12对)，采用4℃含氧合血心脏停跳液间断灌注；温血组(n=12对)，用36℃含氧合血停跳液诱导心脏停跳，冷血维持，终末温血灌注保护心肌。分别于心肺转流(CPB)前(T1)、开放升主动脉后30min(T2)、60min(T3)、停机时(T4)抽取中心静脉血，离心后测定两组血浆心肌肌钙蛋白T(cTnT)、内皮素-1(ET-1)、丙二醛(MDA)、超氧化物歧化酶(SOD)浓度和心脏复跳后的血液动力学指标。结果 CPB后，冷血组血浆中的cTnT、ET-1、MDA较温血组明显增高，心脏复跳后血液动力学指标恢复至基础值水平，温血组优于冷血组，且SOD保持较高的活性。结论 冷温氧合血停跳液联合灌注，能够减轻心肌再灌注损伤，有利于术后心脏功能迅速恢复。     

白胡椒蒸猪心治胃炎

早些年我胃部不适,浑身乏力,医院确诊为慢性胃炎,先后服了多种中西药,都不见效。后经一亲友指点,吃了七只撒上白胡椒粉的猪心,至今未复发。具体做法是：买猪心六七个,白胡椒10克。把猪心切成薄片,白胡椒研末,均匀地撒在猪心片上,然后蒸熟,清晨空腹食用。     

益智健脑靓汤

安神枣仁猪心汤 原料：龙眼肉1Og,酸枣仁1Og,柏子仁1Og,猪心1个,大葱、生姜、料酒、精盐、味精各适量。     

猪心苹果酸脱氢酶的分离纯化及性质

采用组织破碎,二度硫酸铵盐析,DEAE Sepharose F.F.离子交换层析及Sephadex G-75 Fine凝胶过滤等纯化方法,从猪心中提取得到了苹果酸脱氢酶.提取纯化的苹果酸脱氢酶经SDS-PAGE测定显示为单一条带,相对分子质量为34000.酶活回收率为57.99%,最适pH为8.0,最适温度为50℃.     

食疗助睡眠

在绿色食品中，有很多补养安神的物品，如能坚持服用，定能收到良好的效果。下面给您推荐一些有益睡眠的食物及药膳(本刊5月上半期54页《轻松吃出好睡眠》一文也介绍了一些有助睡眠的食物)，失眠者不妨一试。     

保留冠状动脉的猪心螺旋形心室肌带的解剖

Objective Based on the Helical Ventricular Myocardial Band (HVMB) theory proposed by Torrent-Guasp,the ventricular myocardial hand extends from the root of the pulmonary artery to the root of the aorta with two helical coils.This new theory is considered as a revolutionary concept for further understanding the global, three-dimensional and functional architecture of the ven- tricular myocardium. No repot had described techniques for disecting HVMB while keepin～ the integrity of the coronmy artery sys- tern. We explored techniques for dissecting HVMB in swine.Methads 33 fresh swine hearts were randomly divided intoll groups, 3 bearts in each. 160% barium sulfate (type I)suspmmion was injected into the coronary artery system. The coronary arteries were li- gated. The strial tissue was removed following puuing the hearts in boiling water then cooling for several hours. The superficial coro- nary vessels and fat tissue around the atrio-ventricular taxi inter-ventricular sulcus we～'e preserved. Some branches of the left anterior descending artery, distal segment, of posterior descending branch, and middle and distal segment of obtuse marginal branches were mu- tilated appropriately. HVMB dissection was completed with fingers in accordnce with Torrent Guasp' s technique. Results A contin- ued bundle of muscle, originated at the root of pulmonary artery and ended at the root of aorta, was unwrapped along the major dire- tion of the cardiac muscle fiber in all of the 33 hearts with spating of the coronary artery. The swine hearts' ventricular myocandium was cumosed of two loops, with basal loop firm the root of the pulmonart artery to the anterior papillary muscle and apical from the beginning of the anterior papillary muscle to the root tithe aorta. Each loop consisted of two segments: the right segment-coincid- ing with the right ventricular free wall and the left segment-coinciding with the basal d the left ventricular free wall. Posterior papillary muscle, which belongs to the descendant segment, denmrcated the border between the descendent and the ascendant of the HVMB's apical loop. Conclusion Although controversies about the theory of the HVMB remain, we have dissected the HVMB in the swine hearts' ventricular myocardium successfully with sparing of the coronary artery systems. This dissection procedure provides technical information for the studies of associated diseases based on the theory of HVMB.