软骨细胞上清液诱导滑膜间充质干细胞微团培养成软骨

背景：滑膜间充质干细胞在体外具有多向分化的能力,有望成为软骨组织工程中治疗软骨缺损的种子细胞,在其向软骨细胞分化过程中,合适的生长因子起了重要作用。 目的：利用富含生长因子的软骨细胞上清液诱导滑膜间充质干细胞向软骨细胞分化,并对其鉴定。 方法：采用消化法分别获得SD大鼠滑膜间充质干细胞、软骨细胞。收集软骨细胞上清液离心、过滤冻存备用。培养滑膜间充质干细胞至第3代后离心成微团,并用软骨细胞上清液进行成软骨诱导分化,通过形态学观察、免疫组织化学法、RT-PCR检测进行鉴定。 结果与结论：滑膜间充质干细胞使用软骨细胞上清液成软骨诱导21 d后,微团可见似软骨样组织。免疫组化法进行Ⅱ型胶原鉴定,基质能被Ⅱ型胶原染色,细胞染色呈现棕黄色。RT-PCR结果显示诱导后的微团表达软骨特异性基因Ⅱ型胶原和蛋白聚糖。证实软骨细胞分泌的可溶性因子可以诱导大鼠滑膜间充质干细胞向软骨方向分化。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程全文链接：     

石膏铁丝固定法建立兔膝痹模型

BACKGROUND: Currently, the researches on knee plaster immobilization paralysis animal models are popular in the world, but there are some insufficiencies with the knee paralysis animals, for example, poor animal selection, inappropriate plaster selection and pure gypsum instability, which affect the subsequent results.      

巴戟天对骨质疏松破骨细胞表面Ⅰ型跨膜受体蛋白的影响

BACKGROUND: Morinda has been reported to promote the proliferation, the secretion of alkaline phosphatase and osteocalcin, and mRNA expression of transforming growth factor of osteoblasts. However, little information is available addressing the effects of Morinda on receptor activator of nuclear factor-κB expression in osteoclasts in rats with osteoporosis. OBJECTIVE: To study the effects of Morinda on receptor activator of nuclear factor-κB expression in osteoclasts of osteoporosis rats. METHODS: Thirty Sprague-Dawley rats were equally and randomly divided into Morinda and 17β-estradiol groups. Rat models of osteoporosis were established by bilateral ovariectomy, and then 5 mL of Morinda decocta (1.0 mmol/L) and 17β-estradiol (1×10-6 mmol/L) were administered intragastrically to rats in Morinda and 17β-estradiol groups for 3 consecutive months, respectively. Primary osteoclasts were isolated from rats in both groups, and then cultured for 3, 6 and 9 days followed by TRAP staining and cell counting. Bone mineral density of the proximal and distal femur, urine and serum levels of Ca2+ and progesterone, and receptor activator of nuclear factor-κB expression in osteoclasts of rats in both groups were determined. RESULTS AND CONCLUSION: Osteoclast fusion was reduced in Morinda group. In contrast, number of osteoclasts was increased and cells became more mature in the 17β-estradiol group. Bone mineral density of the proximal and distal femur bilaterally, urine and serum levels of Ca2+ and progesterone were significantly increased, while receptor activator of nuclear factor-κB expression was significantly decreased in osteoclasts in Morinda group compared with 17β-estradiol group (P < 0.05). These results indicate that Morinda reduces receptor activator of nuclear factor-κB expression in osteoclasts in osteoporosis rats, thereby inhibiting the development and progression of osteoporosis. 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

支架置入前脑动脉瘤血流场的特性分析

BACKGROUND: Cerebral aneurysm is a kind of mortal hemangioma, and its treatments such as endovascular embolization and clipping both cause high postoperative recurrence rate and mortality. So the stent implantation for cerebral aneurysm is coming into being. OBJECTTVE: To evaluate the hemodynamic parameters after stent implantation into cerebral aneurysm and to provide a novel feasible strategy for clinical treatment. METHODS: A retrospective analysis was preformed based on the CT image data of 11 patients with cerebral aneurysm from the Affiliated Hospital of Xinjiang Medical University. Firstly, the flexible and solid model of cerebral aneurysm was established by the MIMICS and reverse engineering. Secondly, the matching stent model was implanted into the cerebral aneurysm, and then the blood flow structure of cerebral aneurysm was analyzed by the fluid dynamics theory and the Fluent with the method of two-way flow solid coupling. Finally, comparative analysis of the kinetic parameters of cerebral aneurysm before and after implantation, including wall pressure, blood velocity, path line of the blood flow, wall shear stress, wall deformation was conducted, and blood flow characteristics after stent implantation were analyzed under different entrance velocity. RESULTS AND CONCLUSION: After implantation, the wall surface pressure was reduced about 61.1%; the blood flow velocity around the stent and the inside of the cerebral aneurysm was decelerated obviously; under setting 2 000 lines of blood flow, the number of path line of blood flow into the cerebral aneurysm reduced about 75.0%, the maximum wall shear stress decreased about 79.3%, and the maximum wall deformation reduced to a lower level. The entrance velocity was respectively v1=0.1 m/s, v2=0.2 m/s, v3=0.3 m/s and the wall pressure was in a gradient ascent; the wall shear stress increased with the velocity, meanwhile, τzou (left neck of aneurysm) <τzhong (aneurysm )< τyou (right neck of aneurysm). The path lines of blood flow mainly concentrated in the top of the aneurysm, and the blood velocity markedly affected the surface deformation. These results indicate that main hemodynamic parameters are obviously improved after stent implantation into cerebral aneurysm, and the blood velocity should never be neglectful in the treatment process.     

154例自杀患者急诊抢救分析

面对严重的社会、经济和心理压力,任何人都有可能选择自杀。自杀行为己成为现在精神科研究的重要课题。本文总结分析了新疆维吾尔自治区人民医院急诊科154例自杀患者的性别、年龄、职业及受教育程度、有无诱因、自杀方式等。     

玛纳斯县新型农村合作医疗筹资水平及补偿方案分析

1资料来源与方法 1．1资料来源 资料来自自治区农牧区卫生工作协调领导小组办公室主办《新疆新型农牧区合作医疗试点工作信息》，资料由新疆维吾尔自治区卫生厅提供。     

第八届中国药学会施维雅青年药物化学奖评选揭晓

2005年10月14日在河南省郑州市召开的2005年中国药学会药物化学学术会议开幕式上，进行了第八届中国药学会施维雅青年药物化学奖颁奖活动。本届获奖者是中国科学院昆明植物研究所研究员李蓉涛博士、中国海洋大学海洋药物研究所研究员李英霞博士、中国科学院上海药物研究所药物发现与设计中心助理研究员许叶春博士。2005中国药学会施维雅青年药物化学专项研究基金资助获得者是新疆维吾尔自治区药物研究所副研究员吴霞博士。     

新疆维吾尔自治区实施“降消”项目效果浅析

新疆维吾尔自治区地处祖国西北边陲,总人口约2 010万,总面积为166万平方千米.全区共有14个地、州、市,99个县(市、区),849个乡镇,8 868个行政村.     

中医中药中国行启动新疆之旅

2010年6月2日,乌鲁木齐市新疆科技馆的门前广场上,人头攒动,中医专家、维吾尔医专家等为现场群众进行义诊。随着新疆维吾尔自治区政府副主席铁力瓦尔迪·阿不都热西提宣布”中医中药中国行新疆维吾尔自治区活动暨乌鲁木齐市活动启动”,一场在全疆各族群众中展开的中医药和民族医药科普之旅正式开始。     

新疆：医保由“扩面”向“提质”转变

2013年,新疆维吾尔自治区加快健全全面医保体系,继续巩固扩大基本医保覆盖面,职工、城镇居民和新农合医疗三项基本医疗保险参保（合）率稳定在95％以上。城镇居民医保和新农合政府补助标准提高到每人每年290元。适当提高门诊医疗的保障待遇和推进城乡居民大病保险试点。