小儿腹股沟疝微小切口免缝合门诊手术患者的健康教育

目的总结小儿腹股沟斜疝小切口免缝合门诊手术患儿的健康教育。方法口头宣教和发放健康教育单,完善术前准备。术后正确护理,避免并发症的发生。结果68例患儿无出血、感染、复发。结论健康教育为患者解决有关健康问题,同时,提高了护士的业务素质。     

沂水县文化娱乐场所卫生状况调查

随着社会经济的发展，文化娱乐场所也日益繁荣。为掌握其卫生状况，我站对辖区内的影剧院、录像厅、歌舞厅等文化娱乐场所进行了卫生监测，现将１９９３～１９９６年的监测结果统计分析如下。１资料与方法收集整理我站１９９３～１９９６年所有的文化娱乐场所卫生监测资料...     

PCR诊断微小病毒B19感染致儿童造血危象初探

应用聚合酶链反应技术检测儿童骨髓片和血清标本中微小病毒Ｂ１９－ＤＮＡ，在急性造血停滞、周期性粒细胞缺乏症及血液病患儿中均有检出。     

微小病变型肾病综合征患者IL－2的检测及其意义

３２例微小病变型肾病综合征（ＭＣＮＳ）患者，采用￣３Ｈ－ＴｄＲ掺入法测定其外周血Ｔ淋巴细胞产生白介素２（ＩＬ＿２）的浓度，结果显示本病患者Ｔ细胞产生ＩＬ＿２的能力显著降低，与对照组相比差异非常显著（Ｐ＜０．０１），故认为ＭＣＮＳ的发生与Ｔ细胞功能异常有关。     

抗RSV—iRNA对细胞融合病变影响的研究

用RSV-iRNA对BALB/C鼠致敏注射,以融合细胞计数法检测致敏鼠血清的抗融合活性。试验结果表明:RSV-iRNA致敏鼠血清能抑制由RSV引起的典型细胞融合病变,且这种活性可因吸附血清特异抗体而消失。     

HCVRNA阳性的丙型肝炎诊断和治疗探讨

HCVRNA阳性的丙型肝炎46例，均经临床和／或组织学确诊。治疗组24例用IFNa－nl或a－2b3×106IU，隔日一次；对照组22例用一般护肝药物。HCVRNA12周转阴率治疗组为83．3％，对照组为9．0％（P＜0．001）。ALT和AST复常率治疗组也优于对照组（P＜0．05）。随访观察满48周者治疗组15例中HCVRNA持续转阴11例（73．3％）．对照组5例均持续阳性。治疗组中5例做了Ⅰ～Ⅲ型HCV基因分型，结果均为Ⅱ型。同时对急、慢性丙型肝炎的发病过程和病理诊断作了讨论。     

Novel multi-probe RNase protection assay set for detection of endotoxin associated receptors gene expression

Objective: To construct the multi-probe ribonuclease protection assay (RPA) template set to be used for detecting expression patterns of MD-2, TLR4, CD14 mRNAs in human peripheral blood mononuclear cells. Methods : The designed cDNA fragments of the three genes were generated by polymerase chain reaction (PCR)using specific primers and directionally cloned into EcoR I and Hind III sites of expression plasmid pSP72 containing the T7/ promoter, the linearized plasmids was used as template to synthesize anti-sense RNA probes. Then we extracted total RNA from peripheral blood mononuclear cells (PBMC) and detected the dynamic expression patterns of the three genes with RPA method. Results: The proper sequence and orientation of the template set were confirmed by sequencing and the template set was successfully used to assay TLR4, MD-2 and CD14 mRNAs in human PBMC. The results showed that the three detected genes decreased transiently 1-3 hours after 100 ng/ml LPS stimulation. Conclusions: These new RPA multi-probe set provided valuable tool for the simultaneous quantitative determination of expression of TLR4, CD14 and MD-2 mRNAs in both constitutive and inducible types.