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目的用PCR-RFLP方法对rDNA-ITS1片段进行分析,以进一步明确云贵地区是否存在牛带绦虫亚洲亚种,并建立一种快速鉴定方法. 方法 取贵州都匀株(DY)、贵州从江株(CJ)、云南大理株(DL)带绦虫及台湾株(TW)成虫标本,剪取孕节,抽提DNA,PCR扩增rDNA- ITS1片段,分别用4种限制性内切酶MspI、CfoI、AluI、RsaI对扩增片段作酶切分析. 结果 PCR产物经AluI、RsaI酶切后, TW、DL、DY和CJ株RFLP图谱一致;经MspI、CfoI酶切后,TW、DL和DY株RFLP图谱一致,CJ株显著不同. 结论 1)DL和DY株与TW株同属牛带绦虫亚洲亚种;而CJ株是传统牛带绦虫;2)rDNA-ITS1的PCR-RFLP分析方法简便,可以用于带绦虫的分类学研究. 相似文献
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目的分析比较亚洲蹲和西方蹲动作中下肢关节运动学和肌肉激活程度的差异。方法以11名健康成年人为研究对象,采用三维运动捕捉系统、测力台和表面肌电同步采集两种下蹲动作的运动学、动力学和肌肉激活信息,并通过OpenSim计算下肢肌力。结果在膝关节弯曲角度峰值时刻,亚洲蹲骨盆前倾,而西方蹲骨盆后倾;此外,与亚洲蹲相比,西方蹲具有显著较小的髋关节屈角、较大的膝关节屈角、较大的髋关节外展角和内旋角。在自重深蹲的下降期和上升期中,西方蹲的比目鱼肌力峰值均显著大于亚洲蹲,西方蹲的胫骨前肌力均显著小于亚洲蹲,峰值时刻未见统计学差异。结论在亚洲蹲中,胫骨前肌激活和近侧端关节前屈可能有利于稳定;而在脚跟抬起的西方蹲中,比目鱼肌激活显著,但两者近端肌肉激活模式相同。研究结果为临床深蹲康复方案制定或深蹲训练方式的选择提供理论指导。 相似文献
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Ang TL Fock KM Ng TM Teo EK Chua TS Tan J 《World journal of gastroenterology : WJG》2005,11(23):3558-3561
AIM: To examine the clinical spectrum of gastroesophageal reflux disease (GERD) and compare erosive (ERD) with non-erosive (NERD) in terms of clinical, demographic and psychiatric profiles. METHODS: Patients with reflux symptoms were enrolled and stratified to NERD and ERD after endoscopy (LA classification). Patients with ERD presenting with dyspepsia were included. Patients on proton pump inhibitors (PPI) or H2 receptor antagonists before endoscopy were excluded. Demographic data, Helicobacter pylori(H pylori) status and presence of minor psychiatric morbidity (based on General Health Questionnaire-28) were analyzed. RESULTS: Among 690 patients screened, 533 were eligible for analysis (male to female ratio: 3:2; Chinese: 75.4%; Malay: 9.8%; Indian: 14.8%). Clinical spectrum of GERD: N: 40.5%; A: 46%; B: 9.2%; C: 2.1%; D: 0.6%; Barrett's esophagus: 1.7%. Compared to patients with NERD, patients with ERD were significantly older (45 vs 39.4 years), more likely to be male (64.4% vs 53.7%), tended to smoke (19.6% vs 9.7%), less likely to have minor psychiatric morbidity (26.4 vs 46.7%) and were more likely to respond to PPI (79.7 vs 66.8%). There was also a trend towards a higher BMI (24.5 vs 23.5). Race, alcohol consumption and H pylori status were not significant. On multivariate analysis, age and presence of minor psychiatric morbidity remained with significant differences. CONCLUSION: The majority of patients who have typical symptoms of GERD have NERD or mild erosive reflux disease. Compared to patients with erosive reflux disease, patients with NERD were younger and had a higher prevalence of minor psychiatric morbidity. 相似文献
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Yan KK Guirgis M Dinh T George J Dev A Lee A Zekry A 《World journal of gastroenterology : WJG》2008,14(21):3416-3420
AIM:To conduct a multicentre retrospective review of virological response rates in Asians infected with genotype 1 chronic hepatitis C(CHC) treated with combination interferon and ribavirin and then to compare their responses to that among Caucasians.
METHODS:Asian patients infected with genotype 1 CHC treated at 4 Australian centres between 2001 to 2005 were identified through hospital databases.Baseline demographic characteristics,biochemical,virological and histological data and details of treatment were collected.Sustained virological responses(SVR) in this cohort were then compared to that in Caucasian subjects,matched by genotype,age,gender and the stage of hepatic fibrosis.
RESULTS:A total of 108 Asians with genotype 1 CHC were identified.The end of treatment response(ETR) for the cohort was 79% while the SVR was 67%.Due to the relatively advanced age of the Asian cohort,only sixty-four subjects could be matched with Caucasians.The ETR among matched Asians and Caucasians was 81% and 56% respectively(P=0.003),while the SVR rates were 73% and 36%(P 〈0.001) respectively.This difference remained significant after adjusting for other predictive variables.
CONCLUSION: Genotype 1 CHC in Asian subjects is associated with higher rates of virological response compared to that in Caucasians. 相似文献
METHODS:Asian patients infected with genotype 1 CHC treated at 4 Australian centres between 2001 to 2005 were identified through hospital databases.Baseline demographic characteristics,biochemical,virological and histological data and details of treatment were collected.Sustained virological responses(SVR) in this cohort were then compared to that in Caucasian subjects,matched by genotype,age,gender and the stage of hepatic fibrosis.
RESULTS:A total of 108 Asians with genotype 1 CHC were identified.The end of treatment response(ETR) for the cohort was 79% while the SVR was 67%.Due to the relatively advanced age of the Asian cohort,only sixty-four subjects could be matched with Caucasians.The ETR among matched Asians and Caucasians was 81% and 56% respectively(P=0.003),while the SVR rates were 73% and 36%(P 〈0.001) respectively.This difference remained significant after adjusting for other predictive variables.
CONCLUSION: Genotype 1 CHC in Asian subjects is associated with higher rates of virological response compared to that in Caucasians. 相似文献
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汪林 《口腔颌面修复学杂志》2012,(4):235
2012年6月9日—10日,为期两天的第七次中国老年口腔医学年会暨亚洲老年口腔医学学术研讨会在中国老年口腔医学年会暨亚洲老年口腔医学学术研讨会在北京解放军总医院国际会议中心隆重召开。这次会议由中华口腔医学会老年口腔医学专业委员会主办,由解放军总医院口腔医学中心承办,参会代表300余人次,其中有来自韩国、日本、印尼、泰国、 相似文献
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《中华老年口腔医学杂志》2013,(1):10-10
由中华口腔医学会颞下颌关节病学及牙合学专业委员会主办,解放军总医院口腔医学中心承办的第十次全国颞下颌关节病学及牙合学研讨会,暨第三届亚洲颞下颌关节病学大会,国家级继续教育项目《颞下颌关节紊乱病及口颌面疼痛的基础与临床进展》学习班(2013-08-05-017国),定于2013年6月6日-9日在北京亮马河饭店举办。届时将有来自丹麦、日本、韩国、新加坡等10余个国际和地区的专家学者参加本次大会。 相似文献
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