医学论文中统计分析错误辨析与释疑(15)--实验设计原则的正确把握

例1原作者探讨转化生长因子(TCF-β1、β2)作为卵巢内调节物在卵巢激素分泌中的调节作用。在无卵泡刺激素(FSH)条件下，TCF-β对颗粒细胞分泌雌二醇有轻度的刺激作用，而对孕酮分泌的影响不明显。在加入FSH后，发现FSH TGF-β1与FSH FGF-β2分别提高雌二醇的分泌，而抑制FSH     

胃肠道恶性间质瘤8例临床分析

胃肠道恶性间质瘤(Gastro Intestinal Stnmaal Tumors GIST）是一种起源于胃肠壁肌间丛自搏细胞的间叶实体肿瘤。约占所有胃肠道恶性肿瘤的1％～3％，由于缺乏特征性临床征象。极易误诊，自80年代末提出GISI概念以来。有学者已做了较多研究工作，但仍存有多方面争议。现结合我院2000年-2003年间经病理证实的8例患者资料进行分析、探讨。     

上皮样肉瘤的病理学研究

Thirteen cases of epithelioid sarcoma were studied by light microscopy (LM), electron microscopy and immunohistochemical technique. LM revealed a nodular pattern of the tumor cells, which consisted of various degrees of a typical epithelioid cells and plump spindle-shaped cells. Irregular necrosis was seen in the centre of some nodules. Ultrastructurally the tumor cells exhibited abundant intermediate filaments, desmosome-like cell junctions and small intercellular spaces surrounded by microvilli. Immunohistochemically, there was positivity for vimentin and keratin. The author discussed diagnosis, differential diagnosis and histogenesis of epithelioid sarcoma.     

卵巢支持-间质细胞瘤8例临床病理分析

本文总结了8例罕见的卵巢支持-间质细胞瘤。讨论了其病理诊断、组织类型、临床表现和病理类型的关系及恶性程度等。提出了此肿瘤发生于卵巢外是由于阔韧带内残留了胚胎时期部分性索而成,并认为肿瘤囊腔形成与肿瘤细胞脂化有关等见解。     

去细胞喉支架的制备及喉肌细胞再生的可行性研究

Objective To prepare a deeelhilarized whole laryngeal scaffold by utilizing a perfusion-decellularized technique, reseed cells on it, and construct recellularized laryngeal muscles. Methods Perfusion decelluarized larynxes were obtained by common carotid arterious perfusion with detergents. Then they were performed by macroscopic view, histological examination, scanning electron microscopy (SEM) and cartilage viability. Decellularized laryngeal scaffold were then reseeded with inducted mesenchymal stem cells (MSCs). Composites were transferred into greater omentums of rabbits after one day' s adherence and harvested after eight weeks. Macroscopic view, histological examination and immunohistochemistry were performed. Results Perfusion larynxes became transparent after two hours. Histology and SEM indicated that perfnsion method shewed better deculluarized effect. More ventages and collagen fibers but no intact cell or anclei were retained in the decellularized martrix. Porosity measured by Image pro plus 6. 0 was 80. 4% ± 3.2% (x ± s). Chondrocyte vitality assay indicated chondrocyte vitality rate in the perfusion group was 86. 9% ± 1.5% . After eight weeks, vascularization formed and integrated cartilage frameworks still remained. Histological examination could clearly show the presence of muscle bundles and vessels. Immunohistoehemical examination indicated that sarcomeric-α actin expressed positively in corresponding areas. Conclusions It is feasible to reseed MSCs into the decellularized laryngeal muscle matrix for constructing tissue-engineered laryngeal muscles. This in vivo maturation into the omentum could be the first step before in situ implantation of the construct.     

新组合的干扰素疗法可改善乙肝治疗

据医学空间网7月20日报道，英国一项新的研究显示，干扰素与脱氢皮质（甾）醇的联合使用能够改善被乙肝病毒感染的儿童血清转化的时间和速度。