Immunohistochemical features of carcinoma ex pleomorphic adenoma and pleomorphic adenoma in the lacrimal gland

AIM: To investigate C-myc, Ki-67, pan-cytokeratin, and vimentin immunohistochemical features of carcinoma ex pleomorphic adenoma (Ca-ex-PA) and pleomorphic adenoma (PA) in the lacrimal gland in order to find some clues in the differential diagnosis between them. METHODS: We reviewed microscopic slides and clinical records of 64 cases of PA and 15 cases of Ca-ex-PA in the lacrimal gland. Immunohistochemical antibodies for C-myc, Ki-67, pan-cytokeratin, and vimentin were employed. RESULTS: Median age of PA was 43.2y (from 21 to 75). The 35 patients (54.7%) were male and 29 patients (45.3%) were female. For the PAs, the average positivity of C-myc was 4.6%; the average proliferation index of Ki-67 was 3.2%; pan-cytokeratin was positive in ductal cells, and vimentin was positive in myoepithelial cells. Median age of Ca-ex-PA was 54.3y (from 26 to 76). There were 7 male patients (46.7%) and 8 female patients (53.3%). Among 15 Ca-ex-PAs, there were 6 myoepithelial carcinomas, 4 adenocarcinomas, 3 epithelial-myoepithelial carcinomas, and 2 squamous cell carcinomas. For the Ca-ex-PAs, the average positivity of C-myc was 36.4%; the average proliferation index of Ki-67 was 29.2%; pan-cytokeratin was positive in all cases, and vimentin was positive in myoepithelial carcinomas. CONCLUSION: PA has a lower positivity of C-myc and Ki-67, while Ca-ex-PA had a higher positivity of these two biomarkers. These four biomarkers as a set could provide valuable clues in the differential diagnosis between Ca-ex-PA and PA. Our results indicate that the activation of C-myc could play an important role in the pathogenesis of Ca-ex-PA and PA.     

黑水缬草的生药鉴定

目的:对黑水缬草Valeriana amurensis Smir.ex Kom.进行鉴定.方法:性状鉴定与显微鉴定.结果:描述了黑水缬草的性状和显微鉴别特征.结论:研究结果可作为黑水缬草的鏊别依据.     

蒙药材夏至草的生药鉴定

目的:研究蒙药材夏至草的生药鉴定特征,方法,应用来源,性状,显微及薄层鉴别方法.结果:蒙药材夏至草为唇形科植物夏至草Lagopsis supina(Steph),Ik-Gal ex Knorr,的合草,详细描述了其生药鉴别特征.结论:实验结果为鉴别`开发利用该药材提供了鉴定依据.     

铁皮石斛试管培养物多糖含量测定

目的:比较铁皮石斛试管培养物与原药材的多糖含量.方法:用比色法测定石斛多糖的含量.结果:铁皮石斛原药材含多糖量最高,其次是原球茎和试管苗.结论:铁皮石斛原球茎多糖含量比试管苗多糖含量高.     

侵染浙贝母的百合斑驳病毒CP基因的原核表达及抗血清制备

目的:制备抗百合斑驳病毒(Lily mottle virus,LMoV)CP血清,为研究侵染不同植物LMoV之间的血清学关系以及大田检测LMoV奠定基础。方法:根据Genbank报道的百合斑驳病毒CP基因序列设计引物,扩增其外壳蛋白基因并分析序列。将LMoV CP基因插入表达载体pSBET,转化大肠杆菌BL21(DE3)Plys E菌株,通过IPTG诱导表达。经12%SDS-PAGE和5%～20%梯度SDS-PAGE两次纯化CP,免疫小鼠获得抗CP血清,采用Western blot分析抗体的特异性,采用ELISA分析抗体能否与天然LMoV病毒结合。结果:LMoV的CP与目前已报道的LMoV不同分离物CP基因核苷酸序列同源性为95%～99%,氨基酸序列同源性为98%～100%。制备的抗体对CP具有高度特异性,且能够与天然LMoV病毒离子结合。结论:本研究制备的抗血清可以作为百合斑驳病毒的检测。     

密花石斛的HPLC/UV/MS指纹图谱研究

目的 采用HPLC／UV／MS法对密花石斛进行指纹图谱研究。方法 采用Agilent Zorbax SB—C18柱；甲醇和水为流动相进行梯度洗脱；流速为1．0mL／min。结果 得到分离度较好的密花石斛HPLC／UV指纹图谱，标示了9个共有峰，并用HPLC／MS对其部分色谱峰进行了初步定性。结论 该方法可用于密花石斛指纹图谱的测定，并为其全面质量控制提供参考。     

浙贝母中生物碱提取方法以及条件的优化

目的　优化浙贝母中生物碱的提取条件。方法　比较了可能影响生物碱提取率的几个因素 ,并利用数理统计学中的正交试验法优化提取条件。结果　用浙贝母粉末重量的 30倍 75 %乙醇 (W/ V)作为提取溶剂 ,结合摇床振荡法 ,振荡速度为 15 0 r/ min,提取 2 4 h,经乙酸乙酯萃取 ,生物碱的提取率最高。结论　本试验筛选出的提取方法可作为贝母中生物碱提取的一种较好的选择。     

岗梅全长转录组测序及其三萜皂苷生物合成相关基因的挖掘

目的:获得岗梅全长转录组数据库,为深入挖掘岗梅功能基因奠定基础。方法:采用基于PacBio Sequel平台的单分子实时测序技术获取岗梅的根、茎、叶样品的转录组数据,并利用非冗余蛋白(NR)、核苷酸序列(NT)、SwissProt蛋白序列数据库、京都基因与基因组百科全书(KEGG)、真核生物相邻类的聚簇(KOG)、蛋白家族(Pfam)、基因本体(GO)进行注释,分析并鉴定编码三萜皂苷生物合成的关键酶的转录本。结果:全长转录组共获得89629个转录本,其中81313个在公共数据库进行了注释。KEEG代谢通路富集分析表明,623个转录本参与岗梅中三萜皂苷合成相关的3条代谢通路,其中263个转录本编码了三萜皂苷生物合成途径的21个关键酶。此外,还预测了2471个转录因子、40421个简单重复序列位点、22119个长链非编码RNA(lncRNA)和29131个mRNA。结论:丰富了岗梅的转录组数据,并为鉴定参与三萜皂苷和其他次生代谢物生物合成的候选基因提供参考,促进其分子生物学和基因组学的研究。