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Vitreoretinal toxicity of basic fibroblast growth factor 总被引:7,自引:0,他引:7
Hesamodin Borhani Gholam A. Peyman Mohamad H. Rahimy Roger W. Beuerman 《International ophthalmology》1993,17(4):195-199
Basic fibroblast growth factor (b-FGF) is one of the multifunctional growth factors with important therapeutic potential in the field of ophthalmology. It is also implicated in pathogenesis of vitreoretinal proliferative diseases. In the present study, we evaluated its vitreoretinal toxicity by means of clinical observation, electroretinography (ERG), and histopathology after injection of different doses of b-FGF into the vitreous of rabbit eyes. Doses of b-FGF up to 2 g per eye caused no toxicity; however, injection of 4 g or more resulted in sight-threatening vitreoretinal proliferative changes. This information is important for studies aimed at evaluating the therapeutic potential of b-FGF in retinal diseases.Despite some degree of vitreous organization and opacification, retinal folds, and small areas of traction retinal detachment, the amplitudes of ERGs were normal or even increased (hyperpolarization) in eyes which received 8 g of b-FGF. 相似文献
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目的探讨血管生成及组织增生在子宫腺肌病发病机制中的作用。方法应用免疫组化法检测56例腺肌病子宫及26例正常子宫中b-FGF的表达和MVD值。结果b-FGF在腺肌病组异位内膜和对照组正常内膜中的阳性表达率分别为64.3%和38.4%(P<0.05)。在异位内膜周围肌层中的阳性表达率为66.1%,明显高于对照组子宫肌层(42.3%,P<0.05)。MVD与b-FGF在异位内膜及异位内膜周围肌层中的表达水平呈明显正相关(P<0.01)。结论b-FGF的表达增强以及与MVD的正相关性同子宫腺肌病的发病机理有关。* 相似文献
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日本血吸虫童虫细胞体外培养条件的初步研究 总被引:2,自引:3,他引:2
目的 探讨日本血吸虫童虫细胞体外培养条件。方法 选取转铁蛋白、核苷酸、碱性成纤维细胞生长因子(b-FGF)、黄体酮以及非必需氨基酸与维生素组合等5种促进细胞增殖的因子作为考察因素,每种因素选取添加与不添加两个水平,采用正交设计,以RPMI-1640为基础培养液,配制16种条件培养基对日本血吸虫童虫细胞进行体外培养,通过动态观察和AKP组化染色法及直观法分析法获得不同培养条件影响细胞增殖和细胞活力的结果。结果 第2,4,9,13,14孔中的组织出现明显的细胞增殖现象,其细胞AKP检测值也相应较高,各孔中培养细胞的AKP值经直观分析表明:促进细胞增殖最佳因素组合为C181A1,即转铁蛋白、核苷酸、b-FGF。结论 转铁蛋白、核苷酸、b-FGF对日本血吸虫童虫细胞增殖具有明显促进作用。 相似文献
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血管内皮生长因子、碱性成纤维细胞生长因子和一氧化氮合酶3在恶性黑素瘤的表达及其意义 总被引:2,自引:1,他引:2
目的研究血管内皮生长因子(VEGF),碱性成纤维细胞生长因子(b-FGF),一氧化氮合酶3(NOS3)在恶性黑素瘤中的表达及其生物学意义,并探讨其与肿瘤血管形成及淋巴结转移的关系。方法用免疫组化(S-P法)染色技术对45例黑色素瘤石蜡组织标本的VEGF、b-FGF、NOS3及肿瘤内微血管密度(MVD)进行检测和分析。结果VEGF、b-FGF表达与TNM分期、MVD及淋巴结转移有关(P〈0.05),两者共表达与MVD有关(P〈0.01)。NOS3与MVD及淋巴结转移有关(P〈0.05);相关分析显示促血管形成因子(VEGF、b-FGF)与NOS3的表达呈正相关(r=0.2298,P〈0.05)。结论VEGF和b-FGF在促进肿瘤血管形成和转移方面可能起协同作用,并且有NO3的参与;VEGF、b-FGF、NOS的检测对于判断黑色素瘤转移和预后及治疗具有临床应用价值。 相似文献
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H Yamabe H Osawa M Kaizuka S Tsunoda K Shirato F Tateyama K Okumura 《Nephrology, dialysis, transplantation》2000,15(6):872-876
BACKGROUND: Glomerulosclerosis is characterized by glomerular accumulation of extracellular matrix following mesangial cell proliferation. The precise pathomechanism of glomerulosclerosis is still undetermined. Platelet-derived growth factor (PDGF) and basic fibroblast growth factor (b-FGF) are known to be mitogenic for mesangial cells, and interferon gamma (IFN-gamma) is known to have an inhibitory effect on mesangial cell proliferation. We attempted to clarify the role of these cytokines on mesangial matrix production using cultured human fetal mesangial cells (HMC). METHODS: HMC were incubated with these cytokines for 24-72 h and the levels of type IV collagen and TGF-beta in the cell supernatants were measured by enzyme immunoassay. RESULTS: PDGF, b-FGF, and IFN-gamma stimulated type IV collagen production by HMC in a dose- and time-dependent manner. The anti-TGF-beta neutralizing antibody clearly inhibited their stimulatory effect on type IV collagen production. PDGF and b-FGF also stimulated TGF-beta production by HMC in a dose-dependent manner, although IFN-gamma did not. CONCLUSION: PDGF, b-FGF, and IFN-gamma stimulate type IV collagen production in cultured HMC via a TGF-beta-dependent mechanism. 相似文献
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为了探讨垂体肿瘤转化基因(PTTG)与碱性成纤维细胞生长因子(b-FGF)在急性白血病中的表达及其与急性白血病预后的相关性,采用免疫细胞化学染色方法检测53例急性白血病患者及15例非急性白血病患者的PTTG蛋白和b-FGF的表达水平.结果表明,急性白血病组PTTG蛋白和b-FGF表达较非急性白血病组明显升高,两者呈显著性差异(P<0.01).在急性白血病组中,化疗后完全缓解的患者与初发的病人比较有显著差异(P<0.01),并且PTTG蛋白和b-FGF的表达呈正相关(r=0.61,P<0.01).结论:PTTG蛋白和b-FGF在急性白血病中高表达,而且与化疗疗效密切相关,这提示PTTG蛋白与b-FGF的检测对于急性白血病的诊断、疗效评价及预后估计均有一定意义,PTTG与b-FGF参与了急性白血病的发展. 相似文献
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目的:探讨吡格列酮对大鼠碱烧伤后角膜血管内皮细胞生长因子(VEGF),碱性成纤维细胞生长因子(b-FGF)表达的调节作用,以及对碱烧伤后角膜新生血管生长的抑制作用。方法:建立大鼠角膜碱烧伤模型,结膜下注射吡格列酮,观察烧伤后不同时期的角膜新生血管的数量、长度,应用免疫组化和Western blot方法检测碱烧伤后及烧伤治疗不同时期的VEGF,b-FGF的表达变化情况,并与对照组比较。结果:大鼠角膜碱烧伤后2d角膜新生血管开始生长,7~10d达到高峰,吡格列酮治疗后的角膜新生血管的发生延迟,血管的生长受到抑制,VEGF,b-FGF随时间发生变化,表达较对照组下降。结论:局部应用吡格列酮对由碱烧伤引起的鼠角膜新生血管的发生、发展有明显的抑制作用,其机理可能是通过下调促血管生成因子VEGF,b-FGF的表达而实现的。 相似文献
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Julissa Gomez Antonia Vernadakis Susan Kentroti Effrosini Boutou Catherine Hurel Avgi Mamalaki Rebecca Matsas 《Journal of neuroscience research》1998,51(1):119-128
Previous studies have shown that the BM88 antigen, a novel neuron-specific molecule, promotes the differentiation of mouse neuroblastoma (Neuro 2a) cells. In particular, stably transfected, with the BM88 cDNA, Neuro 2a cells overexpressing the BM88 antigen (Neuro2a-BM88 cells) are morphologically distinct from the nontransfected Neuro 2a cells; they exhibit enhanced process outgrowth and a slower rate of division. In this study we used Neuro2a and the morphologically differentiated Neuro 2a-BM88 cells to compare their responsiveness to growth factors. The growth factors we used were nerve growth factor (NGF), basic-fibroblast growth factor (b-FGF), and glial cell-line derived neurotrophic factor (GDNF). In addition, we used glial conditioned medium derived from either newborn mouse cerebral cortex (NBCC) or aged mouse cerebral hemispheres (MACH), as a source of normal glial factors. Because these cells express the cholinergic phenotype, we used choline acetyltransferase (ChAT) activity as a biochemical marker for comparison. A differential responsiveness to these factors was observed between Neuro 2a and Neuro 2a-BM88. The presence of NGF, 25 ng/ml, in the culture medium did not affect ChAT activity in either cell type. In contrast to NGF, in the presence of b-FGF, 5 ng/ml, the transfected cells, Neuro 2a-BM88, responded with a marked increase in ChAT activity. On the other hand, with GDNF, 1 ng/ml, only Neuro 2a cells showed an increase in ChAT activity. Finally, we found no response to the glial conditioned media, although these media contain several growth factors, including b-FGF. In conclusion, our findings show that overexpression of the neuron-specific antigen BM88 in neuroblastoma cells modifies their properties with respect to growth factor sensitivity, and, hence, the Neuro 2a and Neuro 2a-BM88 are suitable cell models to examine the role of growth factors in neuronal differentiation. 相似文献
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目的:探讨参术生肌汤灌洗对青紫兰兔直肠吻合口需粘膜细胞中含碱性成纤维细胞生长因子(b-FGF)、CD68、血管内皮细胞生长因子(VEGF)者比对愈合的影响研究。方法:青紫兰兔30只,在距肛门约5 cm处作直肠吻合,然后随机分成两组。空白组给予常规治疗,实验组于术后第1日起给予参术生肌汤灌洗,每日2次,连续6 d。6 d后处死两组兔子,取含有吻合口的直肠约2 cm。免疫组化检测空白组、实验组吻合口需直肠黏膜细胞中含b-FGF、CD68、VEGF阳性者的比例,研究其对愈合的病理生理影响。结果:空白组、实验组b-FGF阳性细胞数分别为19.73±2.22,27.80±4.10;CD68阳性细胞数分别为19.87±2.20,13.73±2.71;VEGF阳性细胞数分别为18.20±2.81,24.27±2.25;两组间相比,均有明显差异(P0.01)。结论:参术生肌汤可促进直肠吻合口需的b-FGF、VEGF的增殖,同时减轻直肠术后吻合口区的炎症反应(CD68),有助于促进吻合口的愈合。 相似文献