中药整体质量控制标准体系构建的思路与方法

基于中药标准"以我为主,引领国际"的研究目标,阐述了中药质量标准体系构建的科学方法。作者所在实验室多年从事中药质量标准的研究,通过对国内外标准构建方法与思路的学习与实践,提出科学与实用为基本原则,"深入研究,浅出标准"为指导思想,符合中医理论的中药整体质量控制标准体系构建理念。中药标准体系构建主要包括基础研究与标准建立两部分工作,其中深入的基础研究是需要溯本求源,需要全面的药效物质基础及作用机制的深入探讨;同时也对如何浅出科学实用的质量标准,提出了相关建议,包括对照物质,对照图谱以及新的定量分析方法的应用等。     

妊娠期糖化血清白蛋白的影响因素、变动趋势及其正常参考值

目的通过对正常孕妇糖化血清白蛋白（GA）的研究,探讨其影响因素、变动趋势,初步建立妊娠期GA的正常参考值范围。方法选择2010年12月-2013年2月在上海交通大学附属第六人民医院产科门诊产前检查并分娩的孕妇,采用前瞻性研究,以1046名无并发症的正常孕妇为研究对象,采用百分位数方法初步建立GA医学参考值范围;采用t检验、多元线性刚归分析等方法研究其影响因素和变动趋势。结果①正常孕妇1046名,经简单相关性分析得出：在孕12～16、24～28和36～38周GA值与孕前体质量指数（BMI）及孕期体质量增长呈负相关关系（P〈0．05）;与年龄、孕产次、新生儿出生体质最之间无明显相关性（P〉0．05）。多元线性回归分析显示：孕前BMI及孕期体质量增长是影响孕期GA的主要因素。②根据孕前BMI水平,将孕妇分为低BMI组、正常BMI组和超重肥胖组,3组在孕12～16、孕24～28和孕36～38周各组间GA水平差异均有统计学意义（P〈0．05）,并且GA值在各孕期差异均具有统计学意义（P〈0．05）。③1046名正常孕妇,以百分位数法取2．5％～97．5％作为孕期GA正常参考值,其范围为9．20％～14．60％;孕12～16、孕24～28和孕36～38周的GA参考值范围分别为10．20％～15．18％、9．70％～13．98％和8．70％～13．20％。不同孕前BMI正常孕妇不同孕期GA值不同。结论随孕期进展GA水平逐渐降低,9．20％～14．60％可初步作为妊娠期GA的正常参考值范围,但在临床应用中需考虑孕前MBI、孕周和孕期体质量增加等影响因素。     

基于赋权形态学分析的三维面部对称参考平面构建方法

目的: 建立一种基于三维形态学分析的赋权普氏分析(weighted Procrustes analysis,WPA)方法,实现对下颌偏斜患者面部解剖标志点不对称程度量化赋权的三维面部对称参考平面自动化构建。方法: 临床收集下颌偏斜患者30例,基于本体-镜像关联法的普氏分析(Procrustes analysis,PA)算法和自主研发的WPA算法,分别构建每名患者三维面部模型的对称参考平面(实验组一和二),以专家定义的区域迭代最近点(iterative closest point,ICP)算法(选取对称性良好的区域)构建的对称参考平面为“真值平面”,作为参考组。以实验组与参考组的平面角度误差作为评价指标,分别比较两个实验组与参考组的差异,评价WPA算法构建对称参考平面的临床适用性。结果: 30例下颌偏斜患者WPA算法构建的对称参考平面与真值平面的角度误差为1.53°±0.84°,PA算法构建的对称参考平面与真值平面的角度误差为2.06°±0.86°。对于下颌偏斜大于12 mm的患者,WPA算法比PA算法的平均平面角度误差小0.86°。结论: 基于三维面部形态学分析的WPA算法对下颌偏斜面部不对称畸形患者构建三维面部对称参考平面的临床应用效果比传统PA算法有显著提高,初步达到了口腔临床专家级诊断策略的效果。     

唐宋词体式组合规律探赜

唐宋词体式组合规律主要表现在：它是以意群为单位。意群间以收句奇偶的不同呈现三种形式：一为纯奇纯偶收结;一为奇偶交错收结;一为独立意群示拗收结。纯奇纯偶收结。意群间应照应,但可过渡可不过渡,均谐;奇偶交错收结,意群间必须过渡,否则不谐;独立意群示拗收结,意群间不必过渡,仍谐。由纯奇纯偶收结体到奇偶交错收结体再到拗体,反映了词体组合不断复杂优化的创造性发展过程,是唐宋词创调大家名家高度智慧的表现。文中还揭示了照应与多种过渡的方法,及拗体的多种表现形式;并阐明了词体组合规律在节律学与音韵学上的原理。     

Temporal variability of blood lead,mercury, and cadmium levels in elderly panel study (2008–2014)

Background

Biological measurements have been employed as useful biomarkers for exposure. Because of its property of reflecting toxicokinetic differences, however, within-subject variability leads to biased results in epidemiologic studies.

液相分析仪紫外检测器标准物质的研制及不确定度评定

研制了液相分析仪紫外检测器检定用标准物质,简要介绍了标准物质的配制方法,重点介绍了标准物质的均匀性检验和稳定性检验,详细分析了标准物质不确定度的评定,对各级医疗机构药品检验具有重要的指导意义。     

Reference equations of the impulse oscillatory in healthy Thai adults

BackgroundThe ethnicity is significantly under-reported and this may limit the applicability of current impulse oscillometry (IOS) equations to heterogeneous patient populations. Establishing predictive equations for the IOS in the Thai adult population is still required. Therefore, this study aimed to establish reference equations for the IOS in the Thai adult population.MethodsThis retrospective cross-sectional study of IOS parameters in healthy adults aged greater than 20 years old with normal spirometry and who had no chronic respiratory diseases. Pre-bronchodilator (BD) IOS was performed in all subjects. Reference equations were calculated separately for men and women using multivariable linear regression analysis.ResultsA total of 127 subjects (87 men and 40 women) with a mean age of 48.7±17.2 (range, 22–92) years were included. The resistance at 5 Hz (R5), resistance at 20 Hz (R20), and area under reactance curve between 5 Hz and resonant frequency (AX) were significantly higher in women compared to men. The reference equations of the IOS parameters were established for men and women. Age, height, and bodyweight were shown to be the influential predictor as they contributed to the most of IOS indices except for the R5–R20 in men equations. Bodyweight was shown to be the influential predictor as it contributed to the most IOS indices except for the X5 in women’s equations.ConclusionsWe provided the reference equations for the IOS indices in Thai adults. IOS indices including R5, R20, and AX were significantly higher in women compared to men.     

Would the real allele please stand up? Compiling DNA artefacts for the GlobalFiler PCR amplification kit – a South Australian approach

ABSTRACT

This article presents a compendium of DNA artefacts observed using the GlobalFiler and GlobalFiler Express PCR kits (ThermoFisher Scientific) during DNA reference profile assessment at Forensic Science SA (FSSA). The data are currently used to assist with the interpretation of GlobalFiler DNA evidence profiles encountered in the course of routine case work at FSSA. Over 1000 reference profiles have passed observation by reference DNA run readers. An artefact was considered confirmed if it was observed in five or more individual reference samples and could be confirmed upon re-PCR. Artefacts were documented in the following two categories: (1) those with a location independent of true allelic products and (2) those with a location relative to a true allelic product. The artefacts observed were positioned within and outside of allelic designations and were typically less than 1% of the closest allelic product. Artefacts reported here are observed from reference samples only. Their causes are largely unknown and warrant further investigation. Post-developmental artefacts may be encountered due to storage and handling conditions. As such, continued monitoring of reference samples for low level artefacts is warranted.     

六安市健康成人4项凝血指标正常值范围的建立

目的: 通过测定六安市健康成年人群的凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、纤维蛋白原(FIB)、凝血酶时间(TT),建立六安市实验室血浆凝血指标的正常值范围.方法: 使用法国STA-compact全自动血凝仪,采用凝固法对300名健康成年人进行PT、APTT、FIB和TT测定.结果: 初步建立六安市健康成人4项凝血指标正常值范围,PT 11.06～14.24 s;APTT 27.57～42.23 s;FIB 1.91～3.99 g/L;TT 14.05～18.47 s.结论: 指标正常值范围对疾病的预防、诊断、疗效及预后均有重要的指导意义,各实验室应制定适合的指标正常值范围.     

REFERENCE在外贸英语与日常英语中的意义区别

无论是在日常英语还是在外贸英语中 ,refer-ence一词都是一个常见的词。在日常英语中 ,它是一个很活跃的词 ,仅作名词使用 ,加上其有关短语的用法 ,就有 1 0多种不同的意义。而在外贸英语中 ,它又有两种独特的意义 ,这更加大了区别此词意义的难度。1　 REFERENCE在日常英语中的意义　　根据上海译文出版社 1 978年出版的《新英汉词典》,reference的意义分别为 :( 1 )参考 ;( 2 )出处 ,参照 ,参考书目 ;( 3)参照符号 ( =referencemark) ;( 4 )提及 ,涉及 ;( 5 )关系 ,关联。例如 :The parts of a machine all have reference to eachothe…