外阴尖锐湿疣人乳头瘤病毒感染的检测与分型

目的 了解患外阴尖锐湿疣人乳头瘤病毒(HPV)6型和11型的感染情况及PCR方法对尖锐湿疣诊断与分型的临床意义。方法 采用PCR方法测定160例尖锐湿疣患病题组织或分泌物中HPV6／11型及HPVl6／18型的感染率。结果 HPV6／11型感染率为95％(152／160)，HPVl6／18型感染率为5％(8／160)，HPV6／11型 HPVl6／18型混合感染率为1．3％(2／160)。结论 外阴尖锐湿疣患以HPV6型和11型感染为主。PCR方法是一种比较适合临床HPV检测与分型的极为敏感和特异的诊断方法。     

The effect of quinidine on the analgesic effect of codeine

Summary We have studied the hypoalgesic effect of codeine (100 mg) after blocking the hepatic O-demethylation of codeine to morphine via the sparteine oxygenase (CYP2D6) by quinidine (200 mg). The study was performed in 16 extensive metabolizers of sparteine, using a double-blind, randomized, four-way, cross-over design. The treatments given at 3 h intervals during the four sessions were placebo/placebo, quinidine/placebo, placebo/codeine, and quinidine/codeine. We measured pin-prick pain and pain tolerance thresholds to high energy argon laser stimuli before and 1, 2, and 3 h after codeine or placebo.After codeine and placebo, the peak plasma concentration of morphine was 6–62 (median 18) nmol·.l^–1. When quinidine pre-treatment was given, no morphine could be detected (<4 nmol·l^–1) after codeine. The pin-prick pain thresholds were significantly increased after placebo/codeine, but not after quinidine/codeine compared with placebo/placebo. Both placebo/codeine and quinidine/codeine increased pain tolerance thresholds significantly. Quinidine/codeine and quinidine/placebo did not differ significantly for either pin-prick or tolerance pain thresholds.These results are compatible with local CYP2D6 mediated formation of morphine in the brain, not being blocked by quinidine. Alternatively, a hypoalgesic effect of quinidine might have confounded the results.     

Pathogenesis of progressive muscular dystrophy: studies on free radical metabolism in an animal model

Evidence to suggest the presence of abnormal metabolism of oxygen free radicals in progressive muscular dystrophy is presented using an animal model. In the superficial pectoral muscles of dystrophic chickens, enzyme activities regulating the metabolism of oxygen free radicals, i.e., catalase, superoxide dismutases and glutathione peroxidase, were significantly elevated within 1 week of hatching. Activities of related enzymes, i.e., glutathione reductase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase were also elevated. In contrast, the specific activity of phosphofructokinase, the rate-limiting enzyme of the glycolytic pathway, was normal during the first 4-week period. These results suggest that there is an increased turnover of oxygen free radicals in the dystrophic muscle. This concept appears important in a further investigation of the pathogenesis and treatment of progressive muscular dystrophies.     

啤酒酵母在工业贮存期间的变化对生物合成1，6－二磷酸果糖的影响

随着工业贮存期的延长，啤酒酵母细胞的存活率及对葡萄糖连续磷酸化的速率迅速下降，１，６－二磷酸果糖在发酵液中的浓度由贮存前的７５ｇ／Ｌ降至贮存２个星期时的３０ｇ／Ｌ。     

Effect of 6-hydroxydopamine lesions of the amygdala on intravenous cocaine self-administration under a progressive ratio schedule of reinforcement

Bilateral six-hydroxydopamine (6-OHDA) lesions were placed in the amygdala of rats self-administering cocaine (1.5 mg/kg per injection i.v.) under a progressive ratio schedule of reinforcement. Post-lesion access to three doses of cocaine (1.5, 0.75 and 0.37 mg/kg per injection i.v.) revealed a lesion effect only at the highest dose. At this dose, the lesion caused a significant increase in breaking point. No change in the breaking point was produced at the lower two doses. The biochemical results show a significant reduction in dopamine and DOPAC levels within the amygdala and an increase in dopamine within the NACC. In contrast, noradrenaline and serotonin (5-HT) levels were unaffected by the lesion in any of the dissected areas. These results demonstrate that no specific effect on cocaine reinforcement was produced by 6-OHDA lesions of the amygdala. The possibility that the lesion may have attenuated the anxiogenic qualities of the high dose of cocaine is discussed.     

肝动脉化疗栓塞对肝癌细胞黏附分子CD44v6和ICAM-1表达的影响

目的:研究肝细胞癌(hepatocellular carcinoma,HCC)经导管肝动脉化疗栓塞(transcatheter arterial chemoemboli-zation,TACE)后残癌组织细胞黏附分子CD44v6和ICAM-1(intercelluar adhesion molecule-1,ICAM-1)的表达情况。方法:经病理证实的HCC 50例,包括单纯手术切除30例(对照组),TACE术后行Ⅱ期手术切除20例(TACE组)。TACE组患者术前接受1~2次不等的TACE治疗,均按统一规范标准给予化疗药物灌注+栓塞治疗。对标本行免疫组织化学PV-9000染色,其中TACE组取病灶边缘残存肿瘤部分,检测肿瘤组织CD44v6和ICAM-1的表达,将两组结果进行对照分析。结果:对照组和TACE组CD44v6和ICAM-1均有不同程度的表达。对照组CD44v6表达阳性22例(22/30,73.33%),TACE组CD44v6表达阳性13例(13/20,65%),两者间无显著性差异(P>0.05);对照组ICAM-1表达阳性19例(19/30,63.33%),TACE组ICAM-1表达阳性12例(12/20,60%),两者间亦无显著性差异(P>0.05)。对照组和TACE组中CD44v6和ICAM-1表达间均呈正相关(P<0.05)。结论:TACE术后残癌组织CD44v6和ICAM-1仍有较高的表达,TACE并不能有效降低肝癌组织CD44v6和ICAM-1的表达;两者表达呈正相关。     

爆炸伤创面IL-1、IL-6、TNF-α、CRP的测定

[目的]检测兔肢体爆炸伤创面组织IL-1、IL-6、TNF-α和CRP含量。[方法]新西兰大白兔24只,随机分为炸伤后即刻取材组（A组,n=12）和炸后1h取材组（B组,n=12）;0.9g铜壳单质猛黑索金炸药（RDX）以海绵间隔5cm,绑于左下肢股部中段前外侧,电引爆,分别取爆炸中心区（Ⅰ区）、爆炸边缘区（Ⅱ区）、爆震区（Ⅲ区）的肌肉组织,测IL-1、IL-6、TNF-α、CRP的含量并取对侧肢体肌肉组织做对照研究。[结果]炸伤后两组各区标本IL-1、IL-6、TNF-α、CRP含量均比正常组织高,差异显著（P〈0.05）,且从Ⅰ区、Ⅱ区到Ⅲ区,含量依次降低,差异显著（P〈0.05）：A组：Ⅱ区与Ⅲ区的IL-1含量差别有显著意义（P〈0.05）。Ⅰ区与Ⅲ区、Ⅱ区与Ⅲ区的IL-6含量差别有极显著意义（P〈0.01）。Ⅰ区与Ⅲ区的TNF-α、CRP含量差别有极显著意义（P〈0.01）,Ⅰ区与Ⅱ区、Ⅱ区与Ⅲ区的含量差别有显著意义（P〈0.05）。其余各区的含量无统计学差异（P〉0.05）;B组：Ⅱ区与Ⅲ区的Ⅱ-1、IL-6、TNF-α含量差别有显著意义（P〈0.05）。Ⅰ区与Ⅲ区、Ⅰ区与Ⅱ区、Ⅱ区与Ⅲ区的CRP含量差别有显著意义（P〈0.05）。其余各区的含量无统计学差异（P〉0.05）。A、B两组比较,炸后组织中上述因子表达虽有增强,但差异无显著意义（P〉0.05）。[结论]肢体爆炸伤1h内,创面组织中IL-1、IL-6、TNF-α、CRP表达增强,不同区域间表达有差异。     

重组人白细胞介素6对SW872脂肪细胞增殖和凋亡的影响

目的　探讨白细胞介素 6(IL 6)对SW872脂肪细胞增殖及凋亡的影响。方法　体外培养SW872脂肪细胞 ,0 .6mmol/L油酸诱导SW872前脂肪细胞分化 ,化学比色法检测细胞内三酰甘油的总量 ;油红O染色观察细胞内脂肪的聚积 ;MTT法检测不同浓度IL 6对SW872前脂肪细胞增殖活力的影响 ;流式细胞仪分析IL 6对SW872成熟脂肪细胞凋亡的影响。结果　 0 .6mmol/L油酸刺激 72h ,几乎 10 0 %SW872前脂肪细胞分化为成熟的脂肪细胞 ,细胞内三酰甘油的含量明显增加 ,油红O染色胞浆内可见丰富的脂滴 ;5 μg/LIL 6对SW872前脂肪细胞的增殖没有影响 ,10～ 5 0 μg/LIL 6明显抑制SW872前脂肪细胞的增殖 ;IL 6对SW872成熟脂肪细胞的凋亡无影响。结论　IL 6能明显抑制SW872前脂肪细胞的增殖 ,其抑制效应呈剂量依赖性 ,表明IL 6可能与肥胖有关 ,高剂量IL 6可降低肥胖的程度。     

Clinical influence of vagotomy on postoperative acute phase response

BACKGROUND: Although there is increasing evidence suggesting that the vagus nerve functions as a connector between the nervous and immune systems in animals, little is known about the role of the vagus nerve in postoperative acute phase response in humans. MATERIALS AND METHODS: The extent of fever and acute phase protein response and the production of inflammatory cytokine during the early postoperative period were compared among the patients who had undergone total gastrectomy including truncal vagotomy (n = 13), those having distal gastrectomy with division of vagal branches (n = 14), and the patients with vagal nerve preserving gastrectomy (n = 12). RESULTS: There was no significant difference in serum levels of C-reactive protein, alpha-1-antirypsin, and interleukin-6 among the three groups. Also, postoperative maximum body temperature was similar. CONCLUSIONS: Vagotomy did not influence acute phase response after gastric cancer surgery. A multipathway mechanism for acute phase response including the induction of fever is suggested.     

义齿用Ti-6Al-7Nb合金铸件挠度的研究

目的：通过对相同长度、宽度、不同厚度的Ti-6Al-7Nb合金铸件挠度的测量,观察铸件厚度与挠度的关系,为确定Ti-6Al-7Nb合金铸造义齿基托的适合厚度提供实验依据。方法：参照ISO关于义齿基托材料挠度的标准,测量不同厚度Ti-6Al-7Nb合金铸件的挠度,并与纯钛、Ti-6Al-4V合金及Co-Cr合金对比。结果：Ti-6Al-7Nb合金、纯钛及Ti-6Al-4V合金铸件的厚度在0.65mm时可以达到义齿基托挠度的标准;Co-Cr合金在0.57mm时可达到标准要求。结论：在临床制作Ti-6Al-7Nb合金义齿基托时,厚度应不低于0.65mm。