Cerivastatin inhibits fetal calf serunvinduced DNA synthesis in cultured rat mesangial cells

SUMMARY: Inhibition of mevalonate synthesis by several statins has been shown to suppress DNA synthesis in glomerular mesangial cells. In the present study, we investigated the effect of a new statin, cerivastatin, on fetal calf serum (FCS)-induced DNA synthesis of cultured rat mesangial cells. Cultured rat mesangial cells were stimulated by 10% FCS in the presence or absence of cerivastatin and mevalonate. 5-bromo-2-deoxyuridine (BrdU) incorporation was used to assess DNA synthesis. the present study showed that 10% FCS caused marked stimulation of DNA synthesis in the mesangial cells. Cerivastatin inhibited FCS-stimulated BrdU incorporation in a dose-dependent manner. IC₅₀ was approximately 1 umol/L. Exogenous mevalonate, farnesyl pyrophosphate and geranylgeranyl pyrophosphate significantly prevented the inhibitory effect of cerivastatin on DNA replication. It appears that cerivastatin, by inhibiting the synthesis of mevalonate, may suppress DNA synthesis in the mesangial cells.     

抗NI-35重组单链抗体基因cDNA的合成与克隆

目的抗NI-35抗体(anti-NI-35antibody)作为神经再生抑制因子(NI-35)的拮抗剂对神经再生促进作用的研究是近年的热点,研究表明,抗NI-35抗体能促进体内外受损伤神经元的存活和突起生长。我们重新设计并人工合成抗NI-35重组单链抗体(anti-NI-35-scfv)的cDNA克隆构建其表达载体,在原核系统中实现初步表达。方法参照genebank中发表的抗NI-35抗体的轻链重链序列,重新设计适于在大肠杆菌中表达的目的基因片段,将该基因双链分成35个小片段合成,经退火、复性连接成目的片段后,克隆到经过BamHI和HindIII双酶切的克隆载体pUC18中,并转化大肠杆菌DH5a,抽提重组子pUC18/744进行克隆PCR、酶切鉴定及测序分析。结果测序结果证明获得的基因序列与实验设计仅差一个碱基。结论正确合成了抗NI-35重组单链抗体(anti-NI-35-single-chainantibody)为抗NI-35抗体应用于治疗弥漫性轴索损伤提供了实验基础。     

枢椎齿突骨折的治疗进展

枢椎齿突骨折是一种累及寰枢椎区稳定性的严重损伤 ,人们对它的认识是不断变化和深入的。近年来 ,出现了很多新的治疗观念和方法 ,作者对此作一综述     

盐酸伪麻黄碱生产工艺改革

目的：探讨新的盐酸伪麻黄碱生产工艺流程，解决产品质量问题。方法：在研究精制伪麻黄碱的基础上，由伪麻黄碱制备盐酸伪麻黄碱。确定合成条件，考察产品质量和产率。结果：新工艺盐酸伪麻黄碱具有良好稳定性，解决了产品结块问题，且较原工艺的产量有所提高。结论：在原生产工艺基础上的改革有生产应用价值。     

和谐教学的基本原理

本文作者密切联系构建和谐社会的理论与实践，科学地探讨了教学过程自身的和谐、教学过程与教学环境的和谐等实践问题。     

第1产程潜伏期指导对分娩的影响

目的 :观察规范指导对处于分娩第 1产程潜伏期孕妇的影响。方法 :为处于分娩第 1产程潜伏期的 15 0例产妇讲解分娩知识 ,训练助产动作 ,树立自然分娩信心 ,并动态观察产妇的分娩情况。以条件相同 ,未在潜伏期做指导的产妇 15 0例为对照组。结果 :观察组总产程、第 1产程活跃期及第 2产程明显短于对照组 (P <0 0 1) ,新生儿窒息率、剖宫产率明显低于对照组(P <0 0 1)。第 1产程潜伏期、阴道助产率及产后出血发生率两组差异无显著性。结论 :在第 1产程潜伏期对产妇进行规范指导可以缩短产程 ,促进自然分娩 ,降低新生儿窒息发生率。     

Preparation of tritium‐labeled Silybin—a protectant for common liver diseases

Silymarin, the seed extract of milk thistle plant, Silybum marianum, has been used traditionally for the treatment of liver diseases and bile duct infection. Silybin 1 is the main bioactive components of silymarin, consisting a pair of diastereomers: Silybin A and Silybin B. In this article, we report the preparation of tritium‐labeled Silybin, which was accomplished by protection of Silybin as tritylated compound 2 and followed by oxidation of the primary alcohol to its corresponding aldehyde 3 . Subsequent reduction with NaB[³H]₄ and deprotection of the trityl group provided the tritium‐labeled Silybin 4 . Copyright © 2006 John Wiley & Sons, Ltd.     

腰椎横突骨折重要性评价

目的　研究腹部钝性损伤患者腰椎横突骨折的发生与腹部内脏损伤的关系。方法　收集 1997～ 2 0 0 0 -0 7我院收治腹部钝性损伤患者 169例。回顾患者CT片及手术记录 ,总结横突骨折以及内脏损伤的数量及类型。结果　CT显示 81例有横突骨折 ,14 8个横突骨折 ( 43例系单发 ,38例系多发 )。 33例左侧横突骨折 ,2 8例右侧 ,2 0例双侧。L1横突骨折最常见 (n =60 ) ,横突骨折伴发内脏损伤 15例 ,腰椎横突骨折的发生与腹部内脏损伤具有相关性 (Ρ <0 .0 5 )。结论　腰椎横突骨折与腹部内脏损伤具有相关性 ,对临床工作 ,尤其是急救工作具有重要意义     

煤焦沥青烟雾提取物的细胞毒性和遗传毒性研究

采用人外周血淋巴细胞非程序DNA合成(UDS)试验和人胚肺成纤维细胞转化试验,测试了煤焦沥青烟雾提取物(ECTPF)对人体细胞的细胞毒性和遗传毒性。UDS试验结果表明,ECTPF可使淋巴细胞UDS值明显增加,并有剂量一反应关系。引起半数淋巴细胞死亡的浓度(LC50)为33.8μg/ml。细胞转化试验表明,ECTPF能诱发人胚肺成纤维细胞明显的形态学转化,且转化细胞具有部分恶性转化细胞的特性。引起半数人胚肺成纤维细胞生长抑制的浓度为41.3μg/ml。实验结果提示,ECTPF是一种具有细胞毒性和遗传毒性的物质。     

Stochastic models for geriatric in-patient behaviour

Departments of geriatric medicine engage in two distinct formsof clinical activity: acute/rehabilitative and long-stay care.These are organizationally distinct and have very differentresource needs. Current hospital planning models, however, assumethat patients all move through the system at the same rate,thereby ignoring this effect of inherent heterogeneity in patientbehaviour. The present paper describes the movement of patientsthrough geriatric hospitals by a two-stage continuous-time Markovmodel, where the stages represent acute/rehabilitative and long-staypatients respectively. Patients are initially admitted to thefirst stage, from which they may depart from the system, bydeath or discharge, or move into the second stage, from whichthey eventually depart by death or discharge (unlikely). Admissionsare modelled in two ways: either as replacements for departuresor as a Poisson stream. Expressions for the distribution andmovement of numbers of patients are derived and evaluated fordata from a number of hospitals. Such an approach has the advantage,over previous crude models, of taking into account differenttypes of patients and introducing variability, thus making itpossible to extract variances as well as means of numbers ofgeriatric patients requiring hospital care.