不同时间注射PMSG和HCG对小鼠超排卵效果的影响

目的探讨不同时间注射孕马血清激素（PMSG）和人绒毛膜促性腺激素（HCG）对小鼠超排卵效果的影响。方法选用40只6周龄F1昆明小鼠,随机分成4组,分别在同一天的11：00,13：00,15：00,17：00注射PMSG5IU,48h后注射HCG5IU,然后与公鼠合笼;次日晨检查阴栓,阳性者于当日10：00处死取卵,计数受精卵的数量。结果11：00,13：00,15：00和17：00组平均每只获取可用受精卵9．8枚、24．8枚、11．5枚、4．7枚。结论在13：00注射PMSG,48h后注射HCG对小鼠超排效果最好。     

卵巢过度刺激综合征与血管内皮生长因子关系研究

[目的]探讨在超促排卵周期中体外受精患者血管内皮生长因子（VEGF）浓度与卵巢过度刺激综合征（OHSS）发生的相关性及预测价值。[方法]选择60例接受体外受精及胚胎移植者，采用酶联免疫吸附测定法分别测定HCG注射前和注射后取卵日血清及卵泡液中的VEGF浓度。[结果]取卵日OHSS组患者血清和卵泡液中的VEGF浓度均高于高危组及对照组。[结论]VEGF与OHSS的发生密切相关，测定取卵日血清和卵泡液中VEGF的浓度对OHSS的发生有预测价值。     

影响本地杂交黄牛胚胎移植妊娠率的因素分析

本试验选用本地杂交黄牛作为受体,在同一饲养条件下,利用自然发情的方法,移植黑白花奶牛提供的胚胎,移植结果显示:胚胎发育日龄和受体发情日期同步移植,受胎率44.2%,8天受体移植7天桑葚胚,受胎率38.9%,7天受体移植8天囊胚,受胎率35.5%,三者比较差异不显著(P>0.05)。冻胚分别移植A、B、C级黄体的受体,其受胎率分别为40.4%、37.2%、33.3%,三者差异不显著(P>0.05);而鲜胚移植A、B、C级黄体,受胎率分别为48.6%,40.3%,25.8%,A、B级黄体移植受胎率差异不显著(P>0.05),A、C级黄体差异极显著(P<0.01),B、C级黄体差异显著(P<0.05)。不同胚胎质量比较:一、二、三级胚胎移植受胎率分别为50.5%、42.6、25.2%,三者比较,一级、二级胚胎受胎率差异不显著(P>0.05),但二级和三级差异显著(P<0.05),一级和三级差异极显著(P<0.01)。移植单胚和双胚的受胎率分别为25.2%,40.2%,二者差异显著(P<0.05)。     

蓝靛果乙酸乙酯萃取物对小鼠S_(180)实体瘤的抑制作用

[目的]研究蓝靛果乙酸乙酯萃取物(EELC)对小鼠S180实体瘤的抑制作用及血清SOD,MDA, NO,NOS的影响.[方法]将EELC灌胃给予实体瘤模型小鼠,观察对小鼠肿瘤的抑制作用,并用比色法测定血清SOD,NOS,MDA,NO值.[结果]EELC对小鼠S180实体瘤的生长有明显的抑制作用,EELC 大、中、小剂量组的肿瘤抑制率分别为50.99%,39.45%,27.84%;EELC可提高S180实体瘤小鼠血清SOD活力,降低MDA,NO,NOS值.[结论]EELC对小鼠S180实体瘤的生长有抑制作用.     

比利时蓝白花肉牛超数排卵及胚胎移植试验

用FSH +Ctoprostenol药物组合 ,超排处理 1 7头次比利时蓝白花肉牛 ,共采卵 70枚 (头均 4.1 2± 2 .74) ,可用胚 47枚 (头均 2 .76± 2 .30 ) ;以非手术方法移植 34头受体 ,妊娠 1 3头 ,妊娠率 38.2 % ,获 8头犊牛     

多剂量促性腺激素释放激素拮抗剂在体外受精-胚胎移植促超排卵中的应用效果

[目的]探讨促性腺激素释放激素拮抗剂(GnRH-Ant)改良多剂量应用方案在体外受精-胚胎移植(IVF-ET)促超排卵中的使用效果.[方法]回顾性比较分析本中心2006年1～4月接受IVF-ET助孕治疗的患者中采用GnRH-Ant改良多剂量方案应用于26例年龄偏大的患者和采用促性腺激素释放激素激动剂(GnRH-a)长方案降调节方案应用于患者41例,观察其临床效果.[结果]两组促性腺激素的使用天数、受精率、优胚数、HCG日内膜厚度和E2值、流产率、妊娠率分别为(8.58±1.84)d和(7.71±2.06)d、88.17±17.96%和88.91±14.55%、(3.23±1.48) 个和(3.78±2.31)个、(9.81±1.79)mm和(10.37±1.55)mm、(7060.27±4602.02)pmol/L和(7481.85±3852.84)pmol/L、11.54%和14.63%、50%和39.02%,两组之间比较无显著性差异;两组患者年龄、不孕年限、促性腺激素使用量、HCG日LH值、获卵数、MII卵子数分别为(34.73±15.46)和(28.76±3.08)岁、(6.54±4.11)和(4.2±2.08)年、(34.73 ±15.46 ) 支和(19.32±10.45)支、(3.14±3.2 6)IU/L 和(1.21±1.21)IU/L、(6.77 ±3.09) 个和(9.66±3.58)个、6.23±2.79和8.88±3.60个,两组之间比较有显著性差异.[结论]GnRH-Ant联合促性腺激素促超排卵方案较GnRH-a长方案同样有效,尤其对于年龄较大患者比较适用,并可减轻患者的费用.     

两种修正达必佳长方案在IVF-ET中的应用

目的:比较两种修正的达必佳长方案在体外受精-胚胎移植(IVF-ET)和卵母细胞单精子显微注射(ICSI)中的作用。方法:将行IVF-ET和ICSI的512个治疗周期按其停用达必佳的时间分为两组,比较其在诱发排卵中的效果:A组(早停组):上次月经第21天开始隔日一次皮下注射达必佳0.1mg至Gn第4天;B组(改良组):上次月经第21天开始隔日一次皮下注射达必佳0.1mg至注射HCG日。结果:两组Gn天数、ET评分、生化妊娠率、临床妊娠率、早期流产率、OHSS发生率、LH峰发生率比较无显著性差异(P>0.05);A组达必佳支数明显少于B组(P<0.01),且受精率、卵裂率、M2卵母细胞率高于B组(P<0.01)。结论:Gn第4天停用达必佳即可有效抑制内源性LH峰,且能减少达必佳用量,获得与改良长方案相同的临床效果。     

近交系C57小鼠与封闭群昆明小鼠超排卵数的比较

目的 观察C5786小鼠(近交系)和昆明小鼠(封闭群)超排卵后卵母细胞数量的差别.方法 分别对4周龄和7周龄C5786小鼠与昆明小鼠注射孕马血清促性腺激素(PMSG)和人绒毛膜促性腺激素(HCG),进行超排卵处理,观察小鼠超排卵母细胞数量.结果 超排卵母细胞数量为:4周龄昆明小鼠(16.2±2.5)个/只,7周龄(25.2 ±3.1)个/只;4周龄C5786小鼠(9.8±1.2)个/只,7周龄C5786小鼠(18.1±2.2)个/只.超排卵母细胞数量在同种小鼠不同周龄间和同周龄不同小鼠间比较,均有统计学差异(P<0.05).结论 昆明小鼠超排卵母细胞数量高于C5786小鼠.     

Optimization of superovulation in the reproductively mature mouse

Optimum gonadotropin doses and chronology were established for the induction of superovulation in sexually mature hybrid mice (BALB/cBy×C57BL/6By). A regime of 12 IU pregnant mares' serum gonadotropin (PMSG), followed 48 hr later by 20 IU human chorionic gonadotropin (hCG) administered 1 hr before the midpoint of the light cycle (1200), gave the maximum ovulatory response. There was no evidence that endogenous luteinizing hormone influenced the superovulation response to exogenous gonadotropins. Fewer than 50% of zygotes reached the blastocyst stage (90–93 hr post hCG), with the greatest rate of loss at the two-to four-cell stage. Litter size following superovulation was 19.6±0.9. There was no significant difference between the number of blastocysts observed and litter size. Similarly, counts of mature follicles in ovaries prior to hCG stimulation were not significantly greater than the number of secondary oocytes that subsequently ovulated. These data indicate that standard superovulation protocols may require finetuning to maximize productivity and confirm that embryo loss is greatest between the first cleavage division and blastocyst formation.     

Impact of gonadotrophin dose on pre-embryo recovery and development in superovulated mice

A dose—response analysis was performed by stimulatingmice with Humegon, which contains approximately equal amountsof follicle stimulating hormone (FSH) and luteinizing hormone(LH). The effect of increasing stimulation was assessed by monitoringthe number and developmental stages of preembryos flushed fromthe tubes, and the developmental potency of these pre-embryosafter culturing for 72 h. Increasing the stimulation doses resultedin an increased recovery of 2-cell pre-embryos. A maximal plateauof 40–50 2-cell pre-embryos was reached after stimulationwith 15–30 IU FSH/LH. Higher stimulation doses up to 50IU FSH/LH showed no further increase in pre-embryo recovery.Increased stimulation doses did not affect the number of 1-cellpre-embryos or the number of pre-embryos which developed furtherthan the 2-cell stage. An average of 88 ± 1.7% of theflushed pre-embryos were 2-cell pre-embryos. The fraction offlushed pre-embryos which developed into blastocysts after 72h of culturing was constant at all stimulation doses, with anaverage of 81 ± 1.9%. A maximal plateau of 35–40blastocysts was reached after stimulating with 15–30 IUFSH/LH. The group of pre-embryos at less advanced developmentalstages and the group of degenerated pre-embryos showed a smallbut dose dependent increase in number. In conclusion, we foundthat increasing doses of Humegon resulted in an increased recoveryof pre-embryos capable of preimplantation development. Thisincreased recovery occurred despite the high level of LH containedin this FSH/LH stimulation.