抑制剂对酪氨酸酶影响的初步研究

采用酶动力学及聚丙烯酰胺凝胶电泳方法研究了ＮａＨＳＯ３、ＮａＮ３、ＫＳＣＮ、ＤＤＣ对酪氨酸酶的抑制作用。ＮａＨＳＯ３、ＮａＮ３、ＫＳＣＮ为非竞争性抑制作用，随着抑制剂浓度增加，酶活性逐渐减弱。当ＮａＨＳＯ３、ＮａＮ３、ＫＳＣＮ的浓度分别达到１．２ｍｍｏｌ／Ｌ１２。５ｍｍｏｌ／Ｌ１２．５ｍｍｏｌ／Ｌ时，酶活性的抑制率为１００％、５０％、２４％。ＤＤＣ对酪氨酸酶的抑制作用为竞争性抑制作用，其浓度达４ｍｍｏｌ／Ｌ时，酶活性１００％受到抑制。     

Interactions between growth hormone and dexamethasone in skeletal growth and bone structure of the young mouse

Summary The present study investigated the interactions of growth hormone (GH) and glucocorticoid on skeletal growth and bone structure in young mice. The purpose of this study was to examine the possible prevention by GH of the damage inflicted by dexamethasone (Dex) at sites of skeletal growth and ossification. Dex (1 mg/kg) with or without rat GH (rGH) or bovine GH (bGH), 1 mg/kg, was given for 4 weeks, from age 3–7 weeks, to female ICR mice. Tibiae, humerus, and vertebrae were analyzed morphometrically and biochemically. Growth, as determined by the mouse weight, tibial length, and humerus protein content was found to be compromised by dexamethasone. This was prevented by rGH or bGH. The epiphyseal growth plate width, trabecular bone volume, cortical bone width, mineral bone content, and alkaline and acid phosphatase activity were decreased by dexamethasone. These were prevented by rGH or by bGH. The findings of the present study suggest that in the mouse, GH can decrease or even avoid some of the pathological features in growing bones inflicted by high-dose glucocorticoid treatment.     

示波极谱滴定法测定盐酸苯海拉明苯妥英钠及其制剂含量

采用示波极谱滴定法测定了盐酸苯海拉明、苯妥英钠及其制剂含量,所得结果均达到药典标准,与非水滴定法或提取后分光光度法比较,本法具有快速、准确、简单、操作方便、免用有机溶剂的优点。     

有抑郁症史者想象力的研究

实验组为45名缓解的内源性抑郁症患(男25例,女20例)。对照组为15名缓解的双相障碍患者(男8例,女7例),71名正常人(男41例,女30例)。利用作者建立的想象结局法发现:实验组想象力总分显著低于正常人组,特别是当涉及不愉快事件的测题时,单项分亦显著低于正常人。双相障碍组总分亦显著低于正常人。实验组有关性内容的比率得分显著低于正常人。     

肉鸡对不同形态锰源的生物利用率研究

目的 :　研究肉仔鸡对不同形态锰源的相对生物学利用率。方法 :　将 62 4只 1日龄肉公鸡随机分为 1 3组 ,分别饲不添加锰的基础饲粮 (对照组 )或以不同锰化合物添加 60、1 2 0、1 80 mg/ kg锰的试验饲粮 2 1 d。结果 :　肉鸡生长性能各指标和腿病发生率以及肉鸡的跖骨灰锰含量、心肌细胞线粒体中 Mn SOD活性均未受到添加锰源以及锰源与锰水平互作的显著影响 ,但受到添加锰水平的显著影响。肉鸡心肌含锰量和 Mn SOD m RNA水平均未受到添加锰源与锰水平互作的显著影响 ,但受到添加锰源及添加锰水平的显著影响。其中中等和强络合强度有机锰源的生物学利用率明显高于无机硫酸锰和弱络合强度有机锰源。结论 :　中等和强络合强度的复合氨基酸锰对肉仔鸡的生物学利用率明显优于无机硫酸锰和弱络合强度的蛋氨酸锰     

MR susceptometry: an external-phantom method for measuring bulk susceptibility from field-echo phase reconstruction maps

A novel method for estimating the susceptibility of an object by using the magnetic resonance (MR) imaging field distortions in an external-reference water bath next to the object is described. The field measurement was obtained with a phase reconstruction from a gradient-echo acquisition. A field model of an arbitrary object in a static magnetic field was discretely calculated from geometry determined from the magnitude reconstruction. Least-squares estimation yields the susceptibility of the object. Required (and proven) assumptions include validity of superposition, object homogeneity, negligible higher-order field terms, field-model accuracy, geometric-model accuracy, and correlation of gradient-echo phase to field distortion. MR susceptometry estimation of phantoms yielded susceptibility estimates that correlated well with known values (r >.9975). This MR susceptometry method is a first step toward quantitation of iron levels through MR imaging phase information in patients with iron overload.     

高碘摄入对SD大鼠钠/碘同向转运体蛋白表达影响的免疫组化研究

目的:动态观察长期高碘摄入对SD大鼠甲状腺细胞钠/碘同向转运体(NIS)蛋白表达的影响.方法:SD大鼠分别饲以去离子水及不同碘浓度的碘化钾水,给碘后1 d及1,2,4,8周处死.测定尿碘、组织碘含量,免疫组织化学方法观察甲状腺细胞NIS蛋白表达水平.结果:高碘摄入可引起甲状腺滤泡细胞膜表面NIS蛋白表达的下降.短期较低浓度的高碘摄入对NiS蛋白的表达无明显影响,而较高浓度的碘摄入则引起NIS蛋白表达的明显减少;长期高碘摄入对NIS蛋白表达的抑制作用,可在机体自我调节机制作用下得以部分恢复,但NIS蛋白表达仍处较低水平.结论:高碘摄入可导致甲状腺细胞表面NIS蛋白表达下降,随摄碘浓度的增加抑制作用增强;不同浓度的碘摄入对NIS蛋白表达的抑制作用表达时间不同.     

复方卡托普利片中氢氯噻嗪的含量及均匀度测定

用紫外分光度法直接溯定复方卡托普利片中氢氯噻嗪的含量及均匀度。测定波长为272±1nm,平均回收率100.2%,变异系数0.3%,吸收度与氢氯噻嗪的浓度在1～10μg/ml 范围内具有线性关系。     

变换型煤用于制造低含量CO水煤气气化的研究

在小型单管气化反应炉内进行了本题的研究。用粉煤和变换剂制成的型煤进行了水煤气气化反应试验，考察了变换剂种类添加量，气化反应温度，反应时间，蒸汽流量对煤气组成的产气率的影响。结果表明，Ｃａ基变换剂可使煤气ＣＯ含量ψ降低０．１０左右，Ｃａ－Ｎａ复合变换剂还可显著提高煤气产率。     

结节性甲状腺肿及其非典型结节与甲状腺癌细胞核DNA定量研究

作者对结节性甲状腺肿及其非典型结节与甲状腺癌细胞核直径、面积及 DNA 含量检测,发现核直径与面积的(?)和 S 为甲状腺癌组>非典型结节组>对照组(P<0.01);随着细胞增生异型程度的加重,核直径、面积及离散程度随之增加、DNA 含量也显著增加,显示Ⅲ级 DNA 含量细胞出现频率越高恶性的可能性就越大;同时发现各组细胞核不同 DNA 含量的核直径、面积均明显增加,与对照组相比差异生非常显著(P<0.01)。