Compatibility of DAPI and silver staining for combined anterograde and retrograde tracing of neural connections

The geniculocortical and corticogeniculate pathways in hamster were used to test the compatibility of 4′6 diamidino-2 phenylindole 2HCl (DAPI)^6,10 and anterograde degeneration techniques³ for tracing reciprocal connections in the brain. The two methods were compatible within a single brain and, with some loss of sensitivity in the retrograde labeling, within a single section.     

Effect of various chemicals on macromolecular binding during oxidative dealkylation of dimethylnitrosamine by hamster liver microsomes

Differential inhibitory effects of various chemicals on both formaldehyde formation and total macromolecular binding have been examined during oxidative demethylation of [¹⁴C]dimethylnitrosamine (DMN) by hamster liver microsomes. One group of chemicals such as diethyldithiocarbamate (DEDTC), aminoacetonitrile (AAN), 2-[(2,4-dichloro-6-phenyl) phenoxy]-ethylamine (DPEA), azide and ethanol inhibits both HCHO formation and total macromolecular binding. A second group of chemicals such as reduced glutathione, semicarbazide and N-(1-naphthyl)thiourea (NTU) inhibits macromolecular binding without appreciably affecting HCHO formation. Possible mechanisms of these differential inhibitory effects by these chemicals are discussed.     

气相色谱—质谱法测定人血浆中丁螺环酮浓度

建立了血浆样品中盐酸丁螺环酮的气相色谱—质谱（ＧＣ—ＭＳ）联用的定量分析方法。血浆样品用二氯甲烷提取，采用ＧＣ—ＭＳ选择离子和辅助定量离子监测。方法的线性范围为０．５～５０ｎｇ／ｍｌ，回收率达８１％以上，并具有较好的准确度和精密度。该方法应用于盐酸丁螺环酮临床药代动力学试验中血药浓度的测定，获得了准确、可靠的数据。     

In vitro damage of isolated DNA from two brain tumor cell lines induced by a water-soluble antitumor nitrosourea

We report the DNA sites damaged by the antitumor drug, nimustine hydrochloride (ACNU), in highly reiterated DNA sequences of rat glioma cells. A reiterated fragment of 370 base pairs (bp), obtained after Hind III restriction endonuclease digestion of rat glioma C6 or 9L cells DNA, was divided into 167 and 203 bp by subsequent Hae III enzyme reaction. The reaction of end-labelled 167 and 203 bp fragments with ACNU resulted in scission breaks corresponding to the locations of guanine. Moreover, ACNU and subsequent piperidine hydrolysis produced more frequent breaks of the phosphodiester bonds at the guanine positions, thus forming alkali-labile sites. These results indicate that the preferred site of DNA strand scission induced by ACNU is at guanine positions.     

苯乙托品盐酸盐的两种晶型研究

经不同溶剂处理、板层检测、ＮＭＲ，ＭＳ，红外、紫外和元素分析表明，纯净的苯乙托品盐酸盐具有两种不同的熔点，经显微照像，粉末Ｘ－衍射和红外光谱测定结果表明，苯乙托品盐酸盐存在两种不同的晶体结构。     

等导数值光谱法测定寒热痹胶囊中麻黄碱的含量

本文报道用等导数值光谱法测定寒热痹胶囊中麻黄碱的含量。结果表明,该法操作简便、灵敏度高,重现性好。线性范围为0.08～0.48mg/ml,平均回收率为100.4%,变异系数为0.39%.     

高效液相色谱法测定粘膜溃疡膏中盐酸丁卡因的含量

本文采用高效液相色谱法测定乳膏中盐酸丁卡因的含量，测定波长为３１０ｎｍ，其浓度在２０～１００μｇ·ｍｌ－１范围内呈良好的线性关系，ｒ＝０．９９９９，相对标准偏差＜１．０％（ｎ＝５），平均回收率为１０１．６％（ｎ＝５）。方法简单，结果满意，适于医院制剂分析。     

杂种家兔贫血模型的建立

取５０只杂种健康家兔，其中２５只皮下注射盐酸苯肼制备溶血性贫血（ＨＡ）模型，另外２５只行６０Ｃｏ６Ｇｙγ－射线亚致死量全身照射后输注同种异体胸腺淋巴细胞以诱发再生障碍性贫血（ＡＡ，再障）模型。结果ＨＡ组成功率１００％，死亡率５６％；ＡＡ组成功率为９２％。这两种方法制备的贫血模型有很大差异，但发生时间较稳定，实验重复性好，结果可靠，这为ＨＡ和ＡＡ发病机理的探讨及实验研究奠定了基础。     

离子对高效液相色谱法测定抗癌药盐酸米托蒽醌注射液含量

作者用离子对高效液相色谱法测定盐酸米托蒽醌注射液含量。采用Zorbax-ODS柱,甲醇-水-甲酸(450:50:1.5)为流动相,调pH为2.7,加十二烷基硫酸钠为离子对试剂,以Ametantrone为内标,在254nm波长检测,米托蒽醌检测限为1ng(R:N=3:1),在1～10μg范围标准曲线呈线性(r=0.9992),平均回收率为99.62±2.36％(n=10)。注射液中其他组分不干扰测定。     

鸦胆子油乳颗粒剂抗胃溃疡及抗慢性胃炎的作用

鸦胆子油乳颗粒剂1．4，2．8g（鸦胆子油）／kg／d，连续灌胃3天，显著抑制幽门结扎大鼠胃溃疡的形成；1．2g（鸦胆子油）／kg／d，连续灌胃3天，显著抑制阿斯匹林（ASP）所致小鼠胃溃疡的形成；0．6，1．8g（鸦胆子油）／kg／d，连续灌胃3天，对小鼠束水应激性胃溃疡有显著抑制作用；0．5，1．0g（鸦胆子油）／kg／d，连续灌胃9天，显著抑制大鼠醋酸慢性胃溃疡的形成；0．5，1．0g（鸦胆子油）kg／d，连续灌胃3个月，对氨水所致大鼠慢性萎缩性胃炎（CAG）有显著抑制作用．