血小板生成素Ⅱ体内生物活性研究

目的:探讨血小板生成素受体的两种新配体—血小板生成素Ⅱ(TPOⅡ)在体内的生物学活性。方法:以Babl/c纯系小鼠为实验对象,通过腹腔连续7d分组注射提纯的TPOⅡ配体Ⅰ、人工合成的TPOⅡ配体Ⅱ及重组的人血小板生成素,并于第7d末测定小鼠外周血小板数量,分析TPOⅡ体内的生物学活性。结果:在实验第7d,TPOⅡ配体Ⅰ实验组小鼠外周血小板数明显高于同期阴性对照组(P＜0.05),与rhTPO阳性对照组相比则无明显差异(P＞0.05);第14d,TPOⅡ的两个实验组的血小板数均较同期阴性对照组明显升高(P＜0.01);与阳性对照组相比亦无明显差异(P＞0.05)。而且随实验时间的延长,TPOⅡ实验组及阳性对照组血小板数均呈升高趋势。结论:提纯的TPOⅡ配体Ⅰ促进小鼠外周血小板的产生,其升高血小板的能力与rhTPO无明显差别。     

Circulating Monoamine Oxidase B and Phenolsulfotransferase Activities in Essential Hypertensive Patients

Circulating monoamine oxidase (MAO) and phenolsulfotransferase M and P (PST M, PST P) activities were determined in 24 untreated essential hypertensive patients and in 35 age-matched normotehsive healthy controls. These enzymes are involved in the inactivation of monoamines and their metabolites. After lysis of blood, enzymatic activities were determined by radioenzymatic techniques using as substrates [¹⁴C]-beta-phenylethylamine for MAO, 3–methoxy-4–hydroxyphenylglycol for PST M and phenol for PST P. MAO activity measured by this method is fully accounted for by platelet MAO B activity. Women presented higher MAO activity than men. Significantly lower MAO-B activities were     

藻酸双酯钠与细胞因子对巨核细胞生成的调控作用

目的和方法,采用血浆凝块（ＰＣ）,无血清琼脂（ＳＦＡ）培养及（＾１２５Ｉ）－ｂＦＧＦ结合试验等方法研究了ＰＳＳ及ＴＳＰ等８种细胞因子对巨核细胞生成的作用与机制。结果：在ＰＣ系统,ＰＳＳ刺激ＣＦＵ－ＭＫ形成,在ＳＦＡ系统,ＰＳＳ无此作用,ＰＳＳ加强ｂＦＧＦ或再障血清对ＣＦＵ－ＭＫ形成的刺激作用,但ＰＳＳ不增加ｂＦＧＦ与其高亲和力受体的结合,ＴＳＰ及具有肝素结合位点（ＨＢＤ）的ＴＳＰ多肽片段明显抑制Ｃ     

Effects of chronic exposure to mercury and cadmium alone and in combination on the coagulation system of Sprague-Dawley rats

Water contamination with heavy metals may adversely affect our health. High metal levels lead to changes in blood coagulation processes, increasing the risk for cardiovascular disease. Exposure is not limited to a single metal but usually involves a mixture of metals. In this study 24 male Sprague-Dawley rats were exposed to cadmium (Cd) and mercury (Hg), alone and in combination, for 28 days at dosages equivalent to 1000 times the World Health Organization water limits. Scanning electron microscopy analysis revealed that both metals caused platelet activation. Cd significantly increased fibrin fibers thickness and caused aggregation and formation of dense matted deposits (DMDs). Hg reduced fibrin network formation. In the combination group, Hg appeared to augment the effect of Cd, and the presence of extensive DMDs or aggregates between the fibers, with no changes to the actual fibrin thickness, was observed.     

辛伐他汀、维生素E联用对冠心病患者氧化修饰低密度脂蛋白及血小板活化的影响

目的:通过辛伐他汀及其与维生素E联用治疗冠心病合并低高密度脂蛋白-胆固醇(HDL-C)血症患者,探讨HDL-C与氧化修饰低密度脂蛋白(Ox-LDL)及血小板活化的关系。方法:选择冠心病合并低HDL-C血症病人40例,分为辛伐他汀组及辛伐他汀与维生素E联合治疗组,比较治疗前后Ox-LDL、血栓素B₂(TXB₂)、血小板膜表面α-颗粒膜蛋白(GMP-140)水平的变化及与HDL-C的关系。结果:两组血浆HDL-C浓度均较治疗前明显升高,接近正常对照水平。联合治疗组血浆Ox-LDL、TXB₂浓度、GMP-140水平均较治疗前明显降低。辛伐他汀组上述3指标(不包含HDL-C)亦较治疗前明显降低。结论:在体内HDL-C亦能有效抑制LDL的氧化修饰,联合使用辛伐他汀与维生素E提高HDL-C,降低Ox-LDL浓度后,能有效抑制血小板活化。     

Candida albicans elicits protective allergic responses via platelet mediated T helper 2 and T helper 17 cell polarization

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一种新型单克隆抗体导向血栓显像剂的基因工程制备

目的 为简化抗血小板Ｐ－选择素鼠／人嵌合单克隆抗体ＳＺ－５１／Ｈｕ的＾９９ｍＴｃ标记过程,并提高其＾９９ｍＴｃ标记效率,利用金属硫蛋白（ＭＴ）可与金属稳定结合的特点,通过基因工程的手段制备融合蛋白ＳＺ－５１／Ｈｕ－ＭＴ。方法 构建了ＳＺ－５１／Ｈｕ与ＭＴ的重组表达载体,并在小鼠骨髓瘤细胞Ｓｐ２／０中进行了目的融合蛋白的表达。结果 该融合蛋白在Ｓｐ２／０细胞中的表达量为３ｍｇ／Ｌ。对融合蛋白的各项性     

Coated-platelets in ischemic stroke: differences between lacunar and cortical stroke

Background:  Coated-platelets are a subset of platelets with procoagulant potential observed upon dual agonist stimulation with collagen and thrombin. Objective:  The goal was to investigate if coated-platelet production differs between patients with lacunar ischemic stroke and non-lacunar (cortical) ischemic stroke as compared with controls. Patients and methods:  Blood samples from 60 patients with ischemic stroke (20 lacunar and 40 cortical) and 70 controls were analyzed for coated-platelet production. Results:  Coated-platelet production was significantly lower in patients with lacunar stroke (21.8 ± 11.4%, mean ± 1 SD) as compared with either controls (31.6 ± 13.2%, P =  0.008) or patients with cortical stroke (39.4 ± 12.7%, P  <   0.001). The increase in coated-platelets for patients with cortical stroke as compared with controls was also significant ( P =  0.008). Conclusions:  Our results indicate a marked difference in coated-platelet synthesis in lacunar vs. non-lacunar stroke, thereby providing additional support for the existence of distinct pathological processes underlying these two subtypes of ischemic stroke. Further investigation of the role of coated-platelets in stroke, taking into account these preliminary findings, is warranted.     

冠状动脉支架术后优化抗血小板治疗的单中心随机对照预试验

目的 探讨根据实验室检测结果调整、优化冠状动脉支架术后抗血小板治疗方案的可行性和有效性.方法 收集2006年6月-2007年2月在沈阳军区总医院行冠状动脉支架术患者305例,计算机随机分为优化组(n＝154)及标准组(n＝151).氯吡格雷抵抗(CR)定义为氯吡格雷治疗24h后血小板聚集率(PAR)降低＜10%.标准组所有病例均采用标准阿司匹林、氯吡格雷两联抗血小板治疗;优化组非CR患者接受标准两联抗血小板治疗,CR患者在标准两联治疗基础上加服西洛他唑6个月.研究主要终点为随访1年时的死亡、心肌梗死(MI)和缺血性卒中复合终点,次要终点为随访1年时的死亡、MI、缺血性卒中、血运重建及外周动脉闭塞的复合终点.结果 优化组及标准组CR患者分别为41例和33例.优化组CR患者PAR在两联抗血小板治疗基础上加用西洛他唑治疗后(64.5%±12.1%)显著低于单纯应用两联抗血小板治疗(77.5%±12.1%,P＜0.001).随访1年后,优化组主要终点事件风险(5.8%)较标准组(9.3%)下降37%,但差异无统计学显著意义(P＝0.257).优化组心性死亡、心肌梗死、缺血性卒中、血运重建及下肢动脉闭塞的复合终点事件发生率为9.7%,标准组为14.6%,两组比较无显著差异(P＝0.197),但优化组有降低趋势.两组支架内血栓形成及出血事件发生率均无显著差异.优化组CR患者的主要心脑血管缺血事件发生率为19.5%,与标准组CR患者(36.4%)相比有降低趋势,但差异无统计学意义(P＝0.105).结论 根据PAR检测结果调整抗血小板治疗方案可能有利于改善CR患者的长期预后,但其有效性和安全性还需大样本临床研究证实.