喘息灵胶囊Ⅰ(含马兜铃)和喘息灵胶囊Ⅱ(不含马兜铃)的祛痰作用比较

目的比较喘息灵胶囊Ⅰ(原方,含马兜铃)和Ⅱ(新方,不含马兜铃)的祛痰作用。方法用小鼠气管酚红排泄法和蛙食道纤毛运动法比较喘息灵胶囊2个样品的祛痰作用,并用量反应六点法计算其效价比。结果喘息灵胶囊2个样品3个剂量均能明显增加小鼠气管酚红排泄量并增加蛙食道纤毛的运动;小鼠气管酚红排泄量的效价比Ⅰ∶Ⅱ=1∶0.96(95%可信限为0.65-1.41),增加蛙食道纤毛运动的效价比Ⅰ∶Ⅱ=1∶0.95(95%可信限为0.66-1.37)。结论喘息灵胶囊Ⅰ(原方)和Ⅱ(新方)均有明显的祛痰作用,2个样品的效价相当。     

紫外分光光度法测定复方薄荷脑醑中苯酚含量

目的建立复方薄荷脑醑中苯酚含量的测定方法。方法采用紫外分光光度法，测定波长为211nm，以苯酚为对照品。结果苯酚质量浓度线性范围为2．55～15．32μg／mL，r=0．9999，平均回收率为100．1％，RSD为0．14％。结论该法简便、快速、准确，可作为复方薄荷脑醑中苯酚的含量测定方法。     

液晶玻璃毛细管柱色谱和色-质联用分离和鉴定煤焦油馏分

本文用BaCO_3沉积法预处理玻璃毛细管柱,用静态法分别涂以五种液晶固定液(BMBT、BBBT、EPMB、BPBAmB、MPBOB),所得五种液晶玻璃毛细管柱用于分离四种煤焦油馏分——蒽油、洗油、酚油和轻油。发现BBBT和EPMB对蒽油和洗油可得较佳的分离结果;MPBOB毛细管柱可用于分离酚油和轻油。各馏分中分离出的组分数分别为140,100,50,40,流出时间分别为40、30、18和12分钟。各馏分中分离出的组分进行了纯物质对照和色-质联用定性。     

Chemical and statistical considerations in the determination of partition coefficients of weakly ionizable drugs and poisons

Equations have been developed that relate the concentration (or a parameter directly proportional to concentration, such as optical absorbance) of a weakly ionizable solute in a water-immiscible phase, in equilibrium with an aqueous phase, to the pH of the aqueous phase, the partition coefficient of the unionized solute and the phase volume ratio. These relationships have been used in the design of experimental methods for determining partition coefficients, which require measurement of solute concentration in only one phase. Data obtained in this way permit ready recognition of deviations from assumptions made in the development of the model; these assumptions include insolubility of the ionized solute in the water-immiscible phase and lack of interaction between buffer components and solute. Conditions for optimal liquid—liquid extraction of weakly ionizable solutes are more easily recognized. With these techniques, the negative logarithm of the acid dissociation constant (pK′_a) and the logarithm of the octanol—water partition coefficient (log P) have been measured for warfarin (pK′_a = 5.15 ± 0.04; log P = 2.82 ± 0.06), strychnine (pK′_a = 8.29 ± 0.02; log P = 2.23 ± 0.04), phenol (pK′_a = 9.88 ± 0.02; log P = 1.75 ± 0.05), procaine (pK′_a = 8.11 ± 0.04; log P = 1.10 ± 0.08), and ephedrine (pK′_a = 9.92 ± 0.01; log P = 1.65 ± 0.04) at 21°C.     

Improved selectivity in the removal of the tert.-butyloxycarbonyl group

Removal of the tert.-butyloxycarbonyl group by trifluoroacetic acid in the presence of benzyloxycarbonyl groups, benzyl esters and benzyl ethers is rendered more selective by dilution with acetic acid. Trifluoroacetic acid-acetic acid mixtures, however, cause acetylation of hydroxyl groups and also some formation of tert.-butyl esters at free carboxyls. Hence, such mixtures are useful only for the deprotection of intermediates in which the hydroxyl and carboxyl groups are fully blocked. A search for a diluent without such inherent limitation led to the application of a mixture of phenol and p-cresol. Dilution of trifluoroacetic acid with phenols both improved the selectivity in the removal of the tert.-butyloxycarbonyl group and suppressed the alkylation of amino acid side chains as well. A 4:3:3 mixture of trifluoroacetic acid, phenol and p-cresol was found useful in the practical execution of partial deprotection.     

用双波长分光光度法测定大鼠小肠循环液中酚红的含量

采用双波长分光光度法测定了大鼠小肠循环液中酚红的含量 ,方法的线性范围为 1～ 8mg/L ,回收率为 99 88%～ 99 99% (n =5 ) ,日内RSD为 0 5 1%～ 1 2 7% (n =4) ,日间RSD为0 5 8%～ 2 2 9% (n =4) .     

氨酚羟考酮片联合氦氖激光治疗带状疱疹后遗神经痛的临床研究

目的：探讨氨酚羟考酮片联合氦氖激光治疗带状疱疹后遗神经痛（PHN）的疗效.方法：62例PHN患者随机分为2组,治疗组35例,每次口服氨酚羟考酮片0.33g,bid联合氦氖激光治疗qd,疼痛部位照射15分钟,对照组32例,每次口服氨酚羟考酮片0.33g,bid治疗10 d后观察疗效及不良反应.结果：治疗组与对照组治疗有效率比较有统计学意义（P〈0.05）.结论：氨酚羟考酮片联合氦氖激光治疗PHN安全、有效.     

血清丙肝病毒RNA二种提取方法的比较

２４例抗－ＨＣＶ阳性的血清标本，同时以硫氰胍半字符热酚变性方法和蛋白酶ＫＩ肖化法裂解提取丙肝病毒ＲＮＡ（ＨＣＶＲＮＡ），并应用反转录半字符多聚酶链反应（ＲＴ－ＰＣＲ）和套式多聚酶链反应（Ｎｅｓｔ－ＰＣＲ）检测ＨＣＶＲＮＡ。结果表明，硫氰胍半字符热酚法明显优于蛋白酶Ｋ消化法。前者处理标本的阳性检出率为５８．３％，后者处理标本的阳性检出率为３３．３％（Ｐ＜０．０５）；前者操作步骤简便，在试剂的保存等方面优于后者，故前者更适于临床ＨＣＶＲＮＡ的检测     

明目地黄丸中牡丹皮白芍的含量测定

目的:建立紫外分光光度法测定明目地黄丸中牡丹皮、白芍两味中药总丹皮酚含量。方法:按《中国药典》2000年版一部收载六味地黄丸中牡丹皮含量测定方法,以水蒸气蒸馏丹皮酚,通过测定274nm最大吸收波长处紫外吸收进行含量测定。结果:明目地黄丸总丹皮酚的紫外图谱与六味地黄丸中丹皮酚的紫外图谱完全一致,且阴性对照吸收度可忽略不计。结论:本法简便、快速、专属性强、重现性好。     

液晶作为气相色谱固定液的研究

本文总结了作者近年的研究工作。内容包括液晶固定液选择性规律的探讨,液晶色谱柱制备方法的探索,及应用液晶柱分离各种二元取代苯异构体、二甲酚和三甲酚异构体、煤焦油各馏分。所获得的令人满意的分离结果,说明了液晶固定液的独特选择性。