全文获取类型
收费全文 | 53532篇 |
免费 | 5444篇 |
国内免费 | 3751篇 |
专业分类
耳鼻咽喉 | 15篇 |
儿科学 | 1571篇 |
妇产科学 | 158篇 |
基础医学 | 3886篇 |
口腔科学 | 64篇 |
临床医学 | 4552篇 |
内科学 | 17016篇 |
皮肤病学 | 142篇 |
神经病学 | 275篇 |
特种医学 | 2234篇 |
外国民族医学 | 15篇 |
外科学 | 7145篇 |
综合类 | 10062篇 |
现状与发展 | 20篇 |
预防医学 | 2232篇 |
眼科学 | 46篇 |
药学 | 5664篇 |
29篇 | |
中国医学 | 4349篇 |
肿瘤学 | 3252篇 |
出版年
2024年 | 193篇 |
2023年 | 1130篇 |
2022年 | 1894篇 |
2021年 | 2360篇 |
2020年 | 2377篇 |
2019年 | 1985篇 |
2018年 | 1915篇 |
2017年 | 2204篇 |
2016年 | 2640篇 |
2015年 | 2355篇 |
2014年 | 3632篇 |
2013年 | 3568篇 |
2012年 | 3112篇 |
2011年 | 3505篇 |
2010年 | 2686篇 |
2009年 | 2593篇 |
2008年 | 2614篇 |
2007年 | 2643篇 |
2006年 | 2626篇 |
2005年 | 2154篇 |
2004年 | 1690篇 |
2003年 | 1494篇 |
2002年 | 1326篇 |
2001年 | 1225篇 |
2000年 | 1024篇 |
1999年 | 846篇 |
1998年 | 821篇 |
1997年 | 704篇 |
1996年 | 654篇 |
1995年 | 553篇 |
1994年 | 533篇 |
1993年 | 435篇 |
1992年 | 409篇 |
1991年 | 334篇 |
1990年 | 307篇 |
1989年 | 258篇 |
1988年 | 236篇 |
1987年 | 192篇 |
1986年 | 172篇 |
1985年 | 187篇 |
1984年 | 166篇 |
1983年 | 115篇 |
1982年 | 131篇 |
1981年 | 120篇 |
1980年 | 103篇 |
1979年 | 88篇 |
1978年 | 90篇 |
1977年 | 73篇 |
1976年 | 99篇 |
1975年 | 43篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
41.
T. Scholz Ø. Mathisen A. Bergan S. Osnes R. Innes T. Pedersen A. O. Aasen O. Søreide 《Transplant international》1997,10(3):180-184
We have introduced and evaluated several modifications of the conventional venovenous bypass (VVBP) in 29 adult patients
undergoing liver transplantation (OLT). A percutaneous technique for insertion of a jugular venous return cannula and a femoral
vein cannula was applied. The inferior mesenteric vein (IMV) was used for splanchnic decompression, which facilitated dissection
of the recipient liver and allowed portal anastomosis to be performed without disconnecting the portal bypass. A heat exchanger
was introduced into the bypass circuit to prevent heat loss. The percutaneous technique prevented complications related to
dissection in the axilla and groin. Hemodynamic characteristics corresponded to those found using the traditional technique.
Complications related to the VVBP were seen in only one patient in whom the femoral catheter was accidentally introduced into
the femoral artery. We conclude that percutaneous cannulas, use of the IMV for splanchnic decompression and the introduction
of a heat exchanger offer significant benefits and that they are safe and reliable.
Received: 23 August 1996 Received after revision: 14 January 1997 Accepted: 27 January 1997 相似文献
42.
Summary With the use of duplex Doppler ultrasound and color Doppler flow imaging(CDFI), the characteristics of blood supply in neoplasma
were studied in 51 cases of 60 liver tumors, and compared with the results of surgery, pathological examination and hepatic
anerial angiography. The result showed that: 1. Doppler blood flow signals could be detected in all hepatic carcinomas, and
in 10 cases of 18 hemangiomas, significant difference was observed (P<0.001); 2. Doppler blood flow spectra showed pulsatile pattern in 41 of 42 hepatic carcinomas, and in 6 of 10 hemangiomas
(P<0.01); and 3. the peak flow velocity was obviously lower in hemangioma group than in hepatic carcinoma group (20.34±23.93
vs 64.74±30.18 cm,P<0.001). The characteristics of CDFI show that hemangiomas and hepatocellular carcinomas are different. It can, therefore,
be concluded that the blood supply of hepatic carcinomas mainly comes from hepatic arterial system, and is of value in duplex
Doppler ultrasound and CDFI. 相似文献
43.
KYOSUKE YAMAMOTO NOBUHIRO FUKUDA LEI ZHANG TAKAHIRO SAKAI 《Pharmacological research》1996,33(6):337-342
The aim of this study was to examine the effects of dietary fenofibrate (0.05% in the diet) on ketone body production and lipid secretion in isolated perfused rat liver. Feeding with fenofibrate for 7–9 days caused an increased liver weight. Ketone body production was significantly greater in the livers perfused with oleic acid than in those perfused without fatty acid, with the elevation of the ratio ofβ-hydroxybutyrate:acetoacetate indicating an increased redox potential in mitochondrial compartments by exogenous fatty acid. On the other hand, fenofibrate feeding caused a further stimulation of ketone body production from both endogenous and exogenous fatty acid substrates, respectively, with a decreased ratio ofβ-hydroxybutyrate:acetoacetate as compared to respective control livers, indicating a decreased redox potential. Hepatic secretion of triglyceride, but not of cholesterol, was decreased markedly in the fenofibrate-fed rats, especially when oleate was provided, suggesting an inverse relationship between rates of ketogenesis and triglyceride secretion. These results suggest that the altered hepatic metabolism of long-chain fatty acids between oxidation and esterification caused by fenofibrate may thus be a factor responsible for the decreased secretion of triglyceride, hence leading to hypotriglyceridaemiain vivo. 相似文献
44.
大鼠肝细胞Ⅰ,Ⅲ型前胶原基因表达及PDGF的影响 总被引:3,自引:1,他引:2
目的观察大鼠肝细胞Ⅰ,Ⅲ型前胶原基因的表达及PDGF对其表达的影响.方法应用原位杂交技术检测分离培养的SD大鼠肝细胞(n=30)内Ⅰ,Ⅲ型前胶原基因的表达.同时观察10μg/L(n=30)和30μg/L(n=30)PDGF促进前胶原基因表达的作用.测定基因表达颗粒总面积占细胞总面积的百分比,并作比较分析.结果无论正常肝细胞或是在两种浓度的PDGF存在时,肝细胞内均可见到Ⅰ,Ⅲ型前胶原基因的表达.正常肝细胞Ⅰ,Ⅲ型前胶原基因表达面积的百分比(%)为77±19和75±21;加10μg/LPDGF后为115±19和112±10,而加30μg/L后为152±34及181±28,且在后者中表达明显增强(P<005及P<001).结论PDGF在转录水平上促进肝细胞胶原的合成. 相似文献
45.
K. E. Anderson A. M. Dart E. A. Woodcock 《Clinical and experimental pharmacology & physiology》1994,21(2):141-144
1. Global myocariial ischaemia (MI) for periods greater tan 5 min caused an inhibition of phosphatidylinositol specific phospholipase C (PtdIns-PLC) activity. 2. Two min reperfusion following a 20 min MI period, a time point associated with reperfusion-induced arrhythmias, resulted in an activation of PtdIns-PLC activity, dependent on endogenous noradrenaline and mediated via al-adrenoceptors. 3. This 2 min reperfusion response, in contrast to healthy myocardium, resulted in: (i) enhanced PtdIns-PLC activity; (ii) increased sensitivity to endogenous noradrenaline; (iii) rapid increases in inositol(1,4,5)trisphosphate (Ins(1,4,5)P3); and (iv) PLC hydrolysis primarily of PtdIns(4,5)P2, such that the majority of InsP isomers derive from Ins(1,4,5)P3. 4. Together, these data suggest a functional role for Ins(1,4,5)P3 under postischaemic reperfusion conditions, and provide a possible link between al-adrenoceptor stimulation of the PtdIns turnover pathway and reperfusion injury. 相似文献
46.
47.
48.
生物人工肝和肝细胞移植技术需要建立肝细胞库以提供大量具有高度活性和良好功能的肝细胞.肝细胞冻存技术的研究成为实现这一目标的关键,而对冻存保护剂的研究则是肝细胞冻存技术取得突破的主要方向,此文就近些年来国内外肝细胞冻存领域中冻存保护剂的研究进展进行了综述. 相似文献
49.
目的 为构建生物人工肝进行肝细胞的准备。方法 采用胶原酶半原位灌流法分离单个乳猪肝细胞,并对其活力及单层和聚集培养后的白蛋白、尿素合成功能进行检测。结果 采用本方法从每头乳猪中分离到的单个肝细胞数为(3.1±1.5)×10~(10),活性超过95%。在加入激素和生长因子的培养基中单层培养时,肝细胞功能良好,可维持2周左右。而在未加入激素和生长因子的培养中肝细胞虽能存活1周,但功能于24h后即丧失。球形聚集培养可实现肝细胞的大量培养,且生物学活性较单层培养显著提高。结论 采用胶原酶半原位灌注法所得单个乳猪肝细胞基本能满足构建生物人工肝对肝细胞数量的要求。聚集培养接种密度大,细胞生物学活性高,可用于构建生物人工肝。 相似文献
50.
目的 分析创伤性肝破裂外科多种干预治疗的效果。方法 总结分析1982-2002年我院收治创伤性肝破裂76例的救治方法和病死率。其中Ⅲ级以上的严重肝破裂46例(60.53%)。手术治疗56例,手术方式包括单纯修补、清创性肝切除、规则性肝切除、肝周填塞止血;非手术治疗20例。结果 手术组:治愈44例,术后并发症17例均经保守治疗治愈,死亡12例,其中术中死亡2例;非手术组:治愈12例,好转6例,2例失访。全组治愈64例,死亡12例,病死率15.8%。结论 Ⅰ-Ⅱ级单纯性外伤性肝破裂可保守治疗;手术是治疗创伤性肝破裂的主要措施,正确的手术方式,积极处理合并伤,重视综合治疗可提高救治成功率。 相似文献