葛根素微丸制备及含量测定方法

目的：制备葛根素微丸,建立葛根素微丸中葛根素含量测定的方法。方法：离心造粒法制备葛根素微丸。HPLC法测定葛根素的含量,色谱条件为C18柱,以甲醇-水（30∶70）为流动相,流速为1.0 ml/min,检测波长为250 nm。结果：所制备的微丸产率达80%以上,葛根素含量在0.025～0.500μg之间线性关系良好,平均回收率为98.44%,RSD为1.14%。结论：本法简便、准确、重复性好,可用于葛根素微丸的制备和质量控制。     

离心造粒法制备裸花紫珠微丸的工艺研究

目的：考察裸花紫珠微丸制备工艺。方法采用离心造粒法,以微丸粉体学性质如粒径、圆整度、堆密度、脆碎度等及微丸收率为指标,考察影响微丸成型的处方因素和工艺因素。结果微丸的制备工艺为：微晶纤维素与药粉(1：1),以5%PVP-k3060%乙醇溶液为黏合剂,主机转速为200 r/min,以5~25 r/min速度喷5%PVP k-3060%乙醇溶液。结论在优化条件下采用离心造粒法可制得表面光滑,圆整度较好的裸花紫珠微丸,微丸的收率达到85%以上。     

紫外分光光度法测定烟酸微丸中烟酸的含量

目的：测定烟酸微丸中烟酸的含量。方法：采用离心造粒法制备烟酸含药微丸,利用紫外分光光度法建立烟酸的含量测定方法。结果：在2.0-20.0μg.m l^-1范围内,线性关系良好,平均回收率为98.5%,RSD%小于2.5%,烟酸微丸的含药量为70.6%。结论：采用紫外分光光度法测定烟酸含量方法准确、操作简便,快速,重现性好。     

溶血标本在ELISA一步法和二步法中对HBVM测定结果的影响

目的通过实验观察溶血标本在酶联免疫吸附试验（ELISA）一步法和二步法中对乙型肝炎两对半（HBVM）检测结果的影响。方法用80例健康人（HBVM均为阴性）的血液标本,人为造成不同程度溶血,用ELISA一步法和二步法同时检测乙型肝炎病毒表面抗原（HBsAg）、乙型肝炎病毒表面抗体（抗-HBs）、乙型肝炎病毒e抗原（HBeAg）、乙型肝炎病毒e抗体（抗-HBe）、乙型肝炎病毒核心抗体（抗-HBe）,通过对检测结果的观察来分析溶血对检测结果的干扰程度。结果当溶血程度大于或等于10g／L时,ELISA一步法40％以上的标本0D值均大于临界值,差异有统计学意义（P〈0．01）;ELISA二步法仅为3％,差异无统计学意义（P〉0．05）。结论当溶血程度大于或等于10g／L对ELISA一步法检测HBVM的结果有干扰,可造成临界假阳性;ELISA二步法干扰不明显,无统计学意义。     

Increased collagen degradation by experimentally-induced granulation tissue inoculated with bacteria

Abstract Periodontitis is characterized by asymptomatic periodic collagen degradation, which is accompanied by the formation of granulation tissue induced by bacteria. The lesions sometimes contain micro-organisms and/or micro-abscesses that are of unknown significance. The aim of this study was to determine whether bacteria in a sterile granulation tissue could enhance its collagenolytic capacity. The formation of granulation tissue was induced by implanting a cellulose sponge in the subcutaneous tissue in the back of the rat. Bacteria were injected every other day into the sponge from day 8 to day 18. The cell-dependent degradation of a homologous ³H-collagen powder enveloped in the sponge was measured by the radioactivity of the urine excreted 8–18 days after the implantation. The injections increased the excretion of radioactivity by about 40% compared with the controls (n=8, p0.005), but caused no clinical signs of acute infection or inflammation. On day 18, 2 days after the last injection of bacteria, no bacteria or increased cell infiltration were observed in the granulation tissue. The appearance of the latter could not be distinguished from that of the control tissues injected with buffer alone. It seems reasonable to assume that the increased collagen degradation results from enhanced activity of phagocytes, which may also be related to an increased release of tissue-destructive proteases and free oxygen radicals into the extracellular space. In conclusion, brief recurrent episodes of bacteria in granulation tissue can increase its collagen degrading-capacity. The latter may be due to augmented cell activity in the tissue. This response seems to have some features comparable to the pathogenesis of episodic periodontitis, e.g., by mimicking the collagen degradation.     

Basic studies on the application of an artificial esophagus using cultured epidermal cells

In making an artificial esophagus, the transplantation of the epithelialized granulation tube fabricated by organized synthetic material was studied mainly from the viewpoint of preventing anastomotic leakage and stricture formation. The possibility of epithelialization of the inner surface of a granulation tube using cultured epidermal cells was studied in rats and dogs. A stainless steel mesh tube coated with silicon served as the granulation tube. Epithelialization on the inner surface of a granulation tube was evaluated by seeding cultured epidermal cells. A skin sample was treated with dispase and trypsin to collect epidermal cells, which were cultured in a keratinocyte growth medium. Once confluence was achieved, the epidermal cell suspension was harvested using the following methods: trypsin treatment (n=15), mechanical separation with a cell scraper (n=6), and dispase treatment (n=9). The cultured epidermal cell suspension was then seeded into the lumen of the granulation tubes. The attachment of cultured epidermal cells was attained in 2 of 15 cases by trypsin treatment, and in 5 of 9 cases by dispase treatment. No attachment occurred using the cell scraper method. All attached epidermal cells exhibited a cobblestone appearance on the granulation tissue with a tendency toward stratification. These findings show that the inner surface of a steel mesh granulation tube was epithelialized by cultured epidermal cells.     

颅骨蛛网膜颗粒压迹的MRI表现

目的探讨颅骨蛛网膜颗粒压跡的MR I影像特点及诊断价值。方法回顾性分析38例典型颅骨蛛网膜颗粒压跡MR I影像特点。结果 38例中发现蛛网膜颗粒压跡105个蛛网膜颗粒,上矢状窦內发现蛛网膜颗粒78个,15例橫窦24个,直窦及窦汇和下矢状窦各1例共发现3个。15例行MRV中可见靜脉窦充盈缺损45个,MR均表现为长T1长T2信号。结论蛛网膜颗粒是一种正常解剖结构,通常与临床表现无关,有影像学特征表现。     

系统性红斑狼疮病人外周血白细胞TNFRSF12mRNA表达的研究

目的 :了解肿瘤坏死因子受体超家族成员 12 (TNFRSF12 )mRNA在系统性红斑狼疮 (SLE)外周血白细胞中的表达是否异常 ,初步判断TNFRSF12是否为SLE的易感基因。方法 :采用TaqManone stepRT PCR方法 ,分别对SLE病人、正常人和类风湿性关节炎 (RA)病人外周血白细胞TNFRSF12mRNA的表达进行检测。结果 :SLE病人TNFRSF12mRNA的表达形式与表达量和正常人相比均存在差异 ,SLE病人白细胞存在TNFRSF12mRNA的两类形式剪切体 ,分别为编码膜结合蛋白形式和分泌形式的TNFRSF12 ,而正常人只有后者 ,在PHA刺激后才开始同时表达前者。并且TNFRSF12mRNA在SLE病人中的表达量与病情活动度和肾脏累及相关。结论 :提示TNFRSF12在SLE的发病机制中起一定作用 ,因此可能是SLE的易感基因。