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101.
Hanisah Sharif Swati Acharya Gopal Krishna R. Dhondalay Gilda Varricchi Shoshanna Krasner-Macleod Wannada Laisuan Amy Switzer Madison Lenormand Elena Kashe Rebecca V. Parkin Yi Yi Merve Koc Oleksandra Fedina Gemma Vilà-Nadal Gianni Marone Aarif Eifan Guy W. Scadding David J. Fear Mohamed H. Shamji 《The Journal of allergy and clinical immunology》2021,147(2):663-676
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102.
103.
Pierre Marschall Ruicheng Wei Justine Segaud Wenjin Yao Pierre Hener Beatriz Falcon German Pierre Meyer Cecile Hugel Grace Ada Da Silva Reinhard Braun Daniel H. Kaplan Mei Li 《The Journal of allergy and clinical immunology》2021,147(5):1778-1794
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104.
We designed experiments in this study to test the hypothesis suggested by recent purification data that blood basophils comprise two populations of different density, which circulate in numbers characteristic for each human subject. Basophils were separated into two density bands by single step centrifugation on a discontinuous Percoll gradient. Band 1 cells were at the interface between plasma and Percoll of density 1.070 gm/ml. Band 2 cells were at the Percoll 1.070 to 1.080 interface. When the number of band 1 basophils was expressed as a percentage of the total in bands 1 and 2, this relative amount generally remained in a narrow range for blood obtained from the same donor on 3 successive days but differed markedly in different individuals. In a series of leukapheresis experiments, we demonstrated that the percentage of band 1 basophils in postleukapheresis venous blood was strikingly similar to the preleukapheresis value. If basophils that repopulated the leukapheresis-depleted circulation came from the bone marrow, we can conclude that blood levels of basophils in bands 1 and 2 are under physiologic control and that the two types of basophils are released in amounts characteristic for each human subject. Additional evidence for two distinct blood basophil populations was provided by histamine measurements. The histamine content per basophil was consistently higher in cells from band 1 than from band 2, the mean difference between pairs of values for 30 subjects being 0.3 +/- 0.04 pg or about 27% of the band 1 basophil histamine content of 1.1 pg. 相似文献
105.
The Thy-1 antigens or rat brain and thymus have been isolated and chemically characterized, but those of mice have not been identified. Moreover, it is uncertain whether the antigens are glycolipids or glycoproteins. This study with highly purified preparations of gangliosides GM1, 1GD1a, GD1b and GT1b from bovine brain and several ganglioside fractions from mouse brain showed that Thy-1 activity does not reside in gangliosides, but rather in the chloroform-methanol-insoluble residue of brain remaining after extraction of gangliosides. The antigen could be solubilized from this residue with a non-ionic detergent. The antigenic activity of the solubilized preparation was heat-labile but resistant to periodate. The chemical properties of the Thy-1 antigen of mouse brain are discussed. 相似文献
106.
Liposomes could bind and fuse efficiently to human erythrocytes in the presence of HVJ when they contained gangliosides isolated from human erythrocytes. Sialosylparagloboside, which has a terminal sequence of NeuAcα2?3Ga1β1?4GlcNac, has a much higher receptor activity to the virus than GD1a, GD1b, GT1b, and GT1a, all of which contain the terminal sequence of NeuAcα2?3Galβ1?3GalNAc or NeuAcα2?8NeuAcα2?3Galβ1?3GalNAc. The activity of sialosylparagloboside is comparable to that of glycophorin, a major sialoglycoprotein of human erythrocytes, when compared on the basis of the required amount (as sialic acid) of compounds. The high affinity of sialosylparagloboside to the viral HANA protein is also suggested by the finding that it showed high inhibitory activity against HVJ-mediated binding of glycophorin liposomes to erythrocytes. Sialosylparagloboside was also highly susceptible to the viral sialidase, the other biological function of HANA protein. 相似文献
107.
Anti-idiotypic antisera were produced in syngeneic (C57BL/6) mice against a monoclonal anti-Dextran B512 (Dex) antibody (38-13). In radioimmunoassays, anti-idiotypic antibodies were shown to react with the homologous idiotype, while failing to recognize another monoclonal anti-Dex antibody, independently derived from C57BL/6 mice (D-16). Plaque inhibition tests confirmed the specificity of the anti-idiotypic antibodies and revealed that the 38-13 idiotype is expressed by about half of all anti-Dex antibodies produced in C57BL/6, but not in CBA mice. Injection of normal (but not athymic) C57BL/6 mice with low doses of 38-13 monoclonal antibodies, contained culture supernatants or ascitic fluids, resulted in a 10-20 fold increase in the numbers of anti-Dex PFC detected in the spleen 5 days later, the majority of which carried the 38-13 idiotype. 相似文献
108.
109.
Mohammad Lutfur Rahman Masato Aoyama Shoei Sugita 《Anatomical science international / Japanese Association of Anatomists》2008,83(4):239-246
This study was intended to determine the number and density of both retinal ganglion cells and the oil droplets of cone photoreceptor
cells in brown-eared bulbul (Hysipetes amaurotis). For this study birds were killed with proper dose of anesthetic (pentobarbital, 30 mg/kg), and the eyes were removed from
the orbital cavity to isolate the retina. For the ganglion cell study retinal whole-mount specimens were prepared and stained
with 0.1% cresyl violet. The different types of oil droplets were counted from color microphotographs of freshly prepared
retinal samples. The mean total number of ganglion cells was estimated at approximately 2.5×106; with an average density of 16 523 cells/mm2. Two high-density areas, namely the central area (CA) and the dorso-temporal area (DTA), are located in the central and dorso-temporal
retinas, respectively, in bulbuls (24 032 cells/mm2 in the CA; 23 113 cells/mm2 in the DTA). Small ganglion cells persisted in the highest density areas, whereas the largest soma sizes were found in the
lowest density areas of the retina. Four types of different colored oil droplets — red, orange, green and clear — were identified
with an average density of 29 062/mm2. Among the different colors, the green oil droplets had a significantly higher population (13 083/mm2) than the others across the retina. The central retina had a significantly higher number of all types of oil droplets, at
a density of 60 552/mm2. The density and size of the different colored oil droplets were inversely related across the regions of the retina. Taken
together, it is concluded that the CA of the retina is an excellent quality area for visual perception due to peak density
of ganglion cells and oil droplets. Moreover, each specific oil droplet makes a distinct contribution to visual perception,
thereby ensuring that the bird has a retina that best matches its natural environment and feeding behavior. 相似文献
110.
Juan Manuel Leyva-Castillo Mrinmoy Das Emilie Artru Juhan Yoon Claire Galand Raif S. Geha 《The Journal of allergy and clinical immunology》2021,147(6):2305-2315.e3
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