The association of four SNPs in DNA mismatch repair genes with idiopathic male infertility in northwest China

DNA mismatch repair (MMR) plays a critical role in the maintenance of genetic integrity. The failure of MMR in sperm DNA was found in male infertility. However, its aetiology in idiopathic male infertility (IMI) remains unknown. The present study was to investigate whether the four SNPs (rs26279 in MSH3, rs1800734 and rs4647269 in MLH1 and rs175080 in MLH3) in MMR genes were associated with IMI or not. The interactions of the SNPs were also performed to clarify its genetic aetiology. In the present study, 209 clinically diagnosed IMI men and 201 fertile men were recruited. Four SNPs were genotyped by DNA sequencing. It was the first time to investigate the association between rs26279 in MSH3 and IMI. The genotype frequency distribution of rs26279 (A>G) in MSH3 was found to be significantly different between IMI and control (p < 0.05), as well as azoospermia. The rs1800734 and rs4647269 in MLH1 were found to be significantly different between severe oligozoospermia and control groups (p < 0.05). However, rs175080 in MLH3 was not significantly different between IMI and control (p > 0.05). Multifactor dimensionality reduction (MDR) for detecting interactions showed that there were no interactions among the four SNPs on IMI.     

中国西北地区橐吾属植物的种类及药用资源

目的:研究中国西北地区橐吾属植物种类分布及药用资源,探讨它们的分布特点及其药用价值。方法:通过野外调查,标本采集鉴定,馆藏植物标本的查阅和文献资料的整理进行研究。结果:中国西北地区橐吾属植物有29种1变种,且类群相对集中,主要为伞房组、线苞组、橐吾组、蓝灰组的植物;其中有18种作为藏药、维药和民间草药使用,具有止咳化痰、活血化瘀、清热解毒等功效。结论:西北地区橐吾属药用植物资源丰富,值得进一步开发利用。     

活血调脂茶对西北高海拔地区高血脂患者血细胞及血脂的影响

目的：探讨活血调脂茶对西北高海拔地区高血脂患者血细胞及血脂的影响。方法：给予188例常年在西北高海拔地区生活的高血脂患者活血调脂茶（以普通茶的形式泡服）,4周为1个疗程,2个疗程后观察患者服用前后血细胞[红细胞（RBC）、血红蛋白（HGB）、血小板（PLT）],总胆固醇（Tc）,甘油三酯（TG）,高密度脂蛋白胆固醇（HDL—C）,低密度脂蛋白胆固醇（LDL-C）水平变化情况。结果：患者服用活血调脂茶2个疗程后红细胞（RBC）,血红蛋白（HGB）,血小板（PLT）水平明显降低（P〈0．05）;总胆固醇（Tc）,甘油三酯（TG）,低密度脂蛋白胆固醇（LDL-C）水平明显下降（P〈0．01）,高密度脂蛋白胆固醇（HDL-C）水平也有所降低（P〈0．05）。结论：活血调脂茶可调节机体神经-内分泌功能,降低血液黏稠度,抗血小板聚集,并显著改善血脂水平。     

Genetic structure of Kuwaiti population revealed by Y-STR diversity

In this study, a sample of 126 Kuwaiti was analysed using 12 Y-chromosome short tandem repeat (STR) polymorphisms. A total of 101 different haplotypes were identified, among which 87 were individual specific. The high haplotype diversity (0.994) supports the usefulness of Y-STR markers in Kuwaiti population diversity investigation. Our results suggest a close genetic relationship between Kuwait and other populations of the Arabian Peninsula, and an even more pronounced similarity of Kuwaiti populations and Yemenis and Saudi Arabians.     

运用网络药理学探讨桑麻止咳汤治疗西北燥证兼慢性支气管炎的作用机制

[目的] 运用网络药理学探讨桑麻止咳汤治疗西北燥证兼慢性支气管炎的作用机制。[方法] 利用多个数据库获取药物有效成分、疾病靶点,取两者交集基因。通过STRING和DAVID数据库构建蛋白相互作用网络和富集分析,运用Cytoscape建立中药-成分-疾病靶点网络。运用Autodock Vina软件进行分子对接验证。[结果] 筛选得到桑麻止咳汤活性成分287个,药物靶点1 147个,疾病靶点213个。基因本体（GO）富集分析得到93个条目,京都基目与基因组百科（KEGG）通路富集分析确立了49条相关通路。分子对接结果显示药物主要活性成分与核心靶点结合活性良好。[结论] 研究发现,桑麻止咳汤可能通过白细胞介素-17（IL-17）和晚期糖基化终产物及其受体（AGE-RAGE）等信号通路影响肿瘤坏死因子（TNF）、基质金属蛋白酶（MMP9）、白蛋白（ALB）和MMP1等靶蛋白的表达,槲皮素、棕榈酸、山奈酚和芹菜素可能是其发挥多靶点和多途径治疗西北燥证兼慢性支气管炎的主要成分。     

新疆410例慢性阻塞性肺疾病患者中医证型分布规律

目的: 探寻慢性阻塞性肺病(COPD)的中医证型分布规律及特殊证型特点。方法: 分别在新疆南、北疆进行立意抽样调查,南疆选取喀什地区第一人民医院及喀什胸科医院,共收集患者98例,北疆乌鲁木齐地区在新疆维吾尔自治区中医医院呼吸科及其分院、西山社区共调查312例COPD患者。对患者一般情况、生活特点、中医四诊进行临床调查,确定诊断并进行辨证分型。结果: 410例COPD患者中Ⅰ级与Ⅱ级居多,占70%,肺功能改变上第1秒用力呼为容积(FEV₁%)较最大肺活量(FVC%)下降明显;调查显示共计14种证型,单纯实证患者126例,单纯虚证患者167例,虚实夹杂117例;出现频率较高的证型是肾阳虚、肺气虚、痰浊阻肺、风寒袭肺、肾阴虚、西北寒燥证、脾气虚;西北寒燥证主要在Ⅰ级中多见且肺功能相对较轻;出现症状共计51种,其中出现40%的症状是气短、咳嗽、气喘、咽干口干、神疲乏力、咳痰、胸闷、健忘、汗出、畏寒、心慌、腰膝酸软、头晕、形寒怕冷。结论: 新疆患者证型主要以痰、燥、虚、寒为主,根据中医因地制宜、因时制宜的理论,治疗除常规止咳化痰、宣肺平喘外,尚需根据地域特点予以相应的温肺润燥等治疗,及时有效的干预能减少该病的发作次数、延长缓解期时间、迅速缓解症状以提高新疆慢阻肺患者的中医临床疗效。     

Effects of a low severity prescribed fire on water-soluble elements in ash from a cork oak (Quercus suber) forest located in the northeast of the Iberian Peninsula

Wildfire is the major disturbance in Mediterranean forests. Prescribed fire can be an alternative to reduce the amount of fuel and hence decrease the wildfire risk. However the effects of prescribed fire must be studied, especially on ash properties, because ash is an important nutrient source for ecosystem recovery. The aim of this study is to determine the effects of a low severity prescribed fire on water-soluble elements in ash including pH, electrical conductivity (EC), calcium (Ca), magnesium (Mg), sodium (Na), potassium (K), aluminum (Al), manganese (Mn), iron (Fe), zinc (Zn), silica (SiO₂) and total sulphur (TS). A prescribed fire was conducted in a cork oak (Quercus suber) (Q.S) forest located in the northeast part of the Iberian Peninsula. Samples were collected from a flat plot of 40×70 m mainly composed of Q.S and Quercus robur (Q.R) trees. In order to understand the effects of the prescribed fire on the soluble elements in ash, we conducted our data analysis on three data groups: all samples, only Q.S samples and only Q.R samples. All three sample groups exhibited a significant increase in pH, EC (p<0.001), water-soluble Ca, Mg, Na, SiO₂ and TS and a decrease in water-soluble Mn, Fe and Zn. Differences were identified between oak species for water-soluble K, Al and Fe. In Q.S samples we registered a significant increase in the first two elements p<0.001 and p<0.01, respectively, and a non-significant impact in the third, at p<0.05. In Q.R data we identified a non-significant impact on water-soluble K and Al and a significant decrease in water-soluble Fe (p<0.05). These differences are probably due to vegetation characteristics and burn severity. The fire induced a higher variability in the ash soluble elements, especially in Q.S samples, that at some points burned with higher severity. The increase of pH, EC, Ca, Mg, Na and K will improve soil fertility, mainly in the study area where soils are acidic. The application of this low severity prescribed fire will improve soil nutrient status without causing soil degradation and thus is considered to be a good management strategy.     

西北地区黄精属药用植物的调查

报道了西北地区黄精属Polygonatum16种药用植物和它们的分布、生境、变异及药用情况。并提供一个分种检索单。     

中国西北地区早期鼻咽癌调强放疗联合化疗的临床研究

目的 分析生长在西北地区的早期鼻咽癌(T_1~2N_0~1期)患者调强放疗联合化疗的疗效及预后因素。方法 回顾分析2006—2009年本院收治的 58例早期鼻咽癌患者临床资料。Kaplan-Meier法计算生存率,并Logrank法检验和单因素分析。结果 随访率100%,随访满2、3年者分别为50、46例。全组1、2、3年生存率分别为98%、94%、91%。分层分析显示T₁N_0~1、T₂N₀期与T₂N₁期 3年总生存率、局部无复发生存率、无远处转移生存率不同,分别为100%和74%、100%和81%、100%和87%(χ²=5.74、4.95、4.24,P=0.01、0.03、0.04);全组放化疗与单纯放疗的不同,分别为100%与85%、100%与85%、100%与88%(χ²=4.02、4.12、4.84,P=0.04、0.03、0.02);T₂N₁期中放化疗与单纯放疗的也不同,分别为100%与79%、100%与79%、100%与80%(χ²=5.28、4.84、4.72,P=0.03、0.04、0.04)。单因素分析显示N分期、临床分期、放化疗与生存相关(χ²=5.39、5.74、4.02,P=0.02、0.01、0.04)。结论 早期鼻咽癌各亚组中T₂N₁期是远处转移的高危亚组,调强放疗联合化疗可能提高该组病例的无复发生存率、无远处转移生存率及总生存率。