Minor histocompatibility antigen DBY elicits a coordinated B and T cell response after allogeneic stem cell transplantation

We examined the immune response to DBY, a model H-Y minor histocompatibility antigen (mHA) in a male patient with chronic graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplant from a human histocompatibility leukocyte antigen (HLA)-identical female sibling. Patient peripheral blood mononuclear cells were screened for reactivity against a panel of 93 peptides representing the entire amino acid sequence of DBY. This epitope screen revealed a high frequency CD4(+) T cell response to a single DBY peptide that persisted from 8 to 21 mo after transplant. A CD4(+) T cell clone displaying the same reactivity was established from posttransplant patient cells and used to characterize the T cell epitope as a 19-mer peptide starting at position 30 in the DBY sequence and restricted by HLA-DRB1*1501. Remarkably, the corresponding X homologue peptide was also recognized by donor T cells. Moreover, the T cell clone responded equally to mature HLA-DRB1*1501 male and female dendritic cells, indicating that both DBY and DBX peptides were endogenously processed. After transplant, the patient also developed antibodies that were specific for recombinant DBY protein and did not react with DBX. This antibody response was mapped to two DBY peptides beginning at positions 118 and 536. Corresponding DBX peptides were not recognized. These studies provide the first demonstration of a coordinated B and T cell immune response to an H-Y antigen after allogeneic transplant. The specificity for recipient male cells was mediated by the B cell response and not by donor T cells. This dual DBX/DBY antigen is the first mHA to be identified in the context of chronic GVHD.     

Unscheduled care following attendance at Minor Illness and Injury Units (MIU): cross‐sectional survey

Rationale, aims and objectives Minor Illness and Injury Units (MIUs) are becoming a key element in the Urgent Care strategies of Primary Care Trusts. They are intended to both improve access to primary care and to reduce the workload of hospital emergency departments. Their efficiency in resolving patients' needs for health care has been questioned. We sought to describe subsequent health care utilisation among people attending two MIUs in Sunderland, UK. Method Audit of all patients who attended the MIUs during two separate week‐long blocks with General Practitioner (GP) case note review of those who had been either treated and discharged or referred to their GP. Results A total of 1995 patients from 38 practices attended during the study period. 1262 (63.3%) were treated and discharged and 281 (14.1%) referred to the GP. In the subsequent 7 days 336 (21.8%) attended their GP, 37 (2.4%) attended an emergency department and 18 (1.2%) reattended the MIU. Overall, 855 (42.9%) of all attenders required further care, and in 265 (29.9%) this was unscheduled. Conclusion Although most people attending Minor Illness and Injury Units are treated and discharged, subsequent use of health care services is common and in a third of cases is unscheduled. This calls into question the effectiveness of MIUs as an alternative to general practice but may reflect a need for better signposting of patients to the service best suited to their needs.     

Le travail de l’exil à l’adolescence. Dispositif d’étayage groupal et de médiation par la bande dessinée

ObjectiveThe exile is particularly traumatic for adolescents who, in the host country, live the post-migration experience in uncertainty. In order to enhance the continuous sense of being and the imaginary of a group of young unaccompanied minors, and to promote the narrative of their exile, we have set up a group mediated by comics. We strive to understand how and by which processes a comic mediation group could enable the support of the continuous sense of being as well as the imagination in a group of unaccompanied minors.MethodSix (n = 6) adolescents aged 15 to 17 were invited to design a group comic strip. Participants were invited to create a comic in group. The study was divided in three specific times: (1) the first was to invent a story, (2) the second was to do a storyboard and (3) the third and last one consisted in the creation of a comic. The group proceeded in X session and each session ended with a speaking time. We chose to create a comic as a medium because of its formal qualities: the specificity and complexity of its codes seemed appropriate to work on the time and space. Therefore, the breaks and discontinuities as essential parts of the comic structure allowed the participants to work out their temporal and special experiences of exile. As working in group enables to enhance the creative processes, we have designed and set up a comic mediation group.ResultsWe observed that the creation of the comic facilitates the updating of break and loss experiences, because of its formal structure. This sometimes made the group envelope porous and fragile, and damaged the group associative chain. The mediation group allowed participants to express and transform their primary affects through images and words, which led them to go from a fixed time to a historicized time. The comic became an object that represents the individual psychic reality work, the group work but also refers to the common societal reality that concerns those adolescents. Through the comic, they question the adults, the institutions and the environments that are supposed to protect them. The structure of the comic and the group setting helped participants to restore a sense of security. Moreover, the secure and containing space created by the setting allowed the group to enhance its imagination.DiscussionThe setting permitted participants to be in an active position, which supported them in the process of identity reconstruction. They used comics to transform and make sense of a painful part of their story in order to appropriate it. It is necessary to keep doing research on this subject to improve the support given for this young people. Moreover, it is essential to offer them places where they can be accompanied in the crossing of their exile experience.     

Posttraumatic Early Epilepsy and Minor Head Injury

The mechanism of “early epilepsy” sometimes observed after trivial head injury is controversial. Also, the risk of developing late epilepsy after these convulsions, so far, have not been clear.

In order to clarify the mechanism of this posttraumatic early epilepsy after trivial head injury, an attempt was made to observe the role of extracellular potassium ion concentration ([K⁺]_o) in initiation of seizure discharges, in both adult cats and kittens, with K-sensitive microelectrodes.

It was concluded that an increase of [K⁺]_o was not a simple factor for epileptogenesis but minor head injury actually increased seizure slisceptability of immature cat cortex.     

Effect of a minor vitamin A deficiency on the course of infection with <Emphasis Type="Italic">Ascaridia galli</Emphasis> (Schrank, 1788) and the resistance of chickens

Summary The effect of vitamin A deficiency was studied in chickens infected with 500 Ascaridia galli eggs and controls. Diet 1 (deficient, 500 IU vitamin A or 172 μg retinol acetate per kg diet), Diet 2 (deficient, 1000 IU vitamin A or 344 μg retinol acetate per kg diet) and Diet 3 (sufficient, 1500 IU vitamin A or 516 μg retinol acetate per kg diet) were assigned to 46 chickens each. Clinical signs, weight gains, livers’ weights, vitamin A levels, worm burdens and parasite eggs’ excretions were recorded. Infected chickens had lower weight gains than the controls fed alike. Chickens given Diet 1 stored lesser vitamin A in liver than those fed Diet 3. Although worm counts in the 3 groups did not differ significantly, chickens fed Diet 1 excreted more A. galli eggs than those fed the 2 other diets. Female worms harboured by chickens fed Diet 1 had higher fecundity at week 5 pi than those of chickens fed Diet 2. Results indicated that Vitamin A is important for poultry in the moderation of the infection with A. galli.     

Cognitive dysfunction in beta-thalassemia minor

Beta-thalassemia minor is a common, hereditary, and mostly symptomless disease. Previous studies have shown that lower hemoglobin values are associated with poorer cognitive functions. We aimed to evaluate the cognitive function in patients with beta-thalassemia minor. Thirty-two male subjects with beta-thalassemia minor and 32 sex-, age-, and education status-matched healthy subjects were enrolled in the study. Blood tests and P300 potentials were carried out. P300 potential latency in all patients was significantly longer than those in the control group (337.63+/-34.89 msec and 310.66+/-14.30 msec, respectively; t 4.046, P<0.001). The amplitudes of P300 in patient group were significantly lower than those in the control group (5.19+/-3.59 microV and 9.81+/-3.33 microV, respectively; t 5.349, P<0.001). In this study, we have found that P300 potentials are adversely affected for cognitive functions in patients with beta-thalassemia minor.     

Size,location and patency of the minor duodenal papilla as determined by dye‐injection endoscopic retrograde pancreatography

Background: The accessory pancreatic duct (APD) sometimes is developmentally obliterated near the duodenum. We evaluated patency of the minor duodenal papilla by dye‐injection endoscopic retrograde pancreatography to determine whether patency was related to papillary size and location. Methods: We injected 2–3 mL of contrast material containing indigocarmine into the main pancreatic duct via an endoscopic catheter in 104 patients. It was endoscopically observed whether dye was extruded from the minor papilla. Size of the minor papilla and distance from the orifice of the major duodenal papilla to the apex of the minor papilla were measured endoscopically with measuring forceps. Results: The APD was patent in 56 of 104 cases (54%). Size of the minor papilla varied considerably from 3 to 6 mm, but showed no correlation with patency. Half of the patients with chronic pancreatitis (6/13) had the minor papilla larger than 6 mm. In cases where the terminal APD had a cudgel or tapering‐off configuration, the minor papilla was larger than in cases where the duct had a stick shape. The minor papilla was patent in 9 out of 10 cases (90%) when it was near the major papilla (≤ 1.5 cm). Frequency of a patent minor papilla was 16 out of 33 (48%) when it existed 1.5 to 2.0 cm from the major papilla, and 31 out of 61 (51%) when the distance was more than 2.0 cm. Conclusions: The minor papilla was more frequently patent when it was close to the major papilla (P < 0.05).     

Disparity for a newly identified minor histocompatibility antigen, HA-8, correlates with acute graft-versus-host disease after haematopoietic stem cell transplantation from an HLA-identical sibling

We recently identified a new minor histocompatibility antigen, termed HA-8, which is presented by human leucocyte antigen (HLA)-A*0201 or HLA-A*0202 and expressed ubiquitously among tissues. A retrospective analysis of 577 Caucasian patients with HLA-A*0201 or A*0202 who had received a haematopoietic stem cell transplant from a human leucocyte antigen (HLA)-identical sibling was conducted to determine whether HA-8 disparity correlated with clinical outcome. HA-8 disparity was detected in 72 recipients, and grades II-IV graft-versus-host disease (GVHD) occurred in 46 (64%), compared with 251 (50%) of the 503 patients without HA-8 disparity. After adjusting for known risk factors for acute GVHD, this difference was statistically significant (odds ratio, 1.8; 95% confidence interval, 1.0-3.1; P = 0.04). However, the hazards of clinical extensive chronic GVHD, overall mortality and recurrent malignancy were not statistically significantly different between the two groups. These data suggest that the increased risk of acute GVHD associated with recipient HA-8 disparity was not sufficient to change other clinical outcomes.