石辛含片联合碘甘油治疗智齿冠周炎的疗效观察

目的探讨石辛含片联合碘甘油治疗智齿冠周炎的临床疗效。方法选取2016年4月—2017年4月在解放军第101医院进行诊治的智齿冠周炎患者160例,随机分为对照组(80例)和治疗组(80例)。对照组患者将碘甘油涂于牙周袋,3次/d。治疗组在对照组的基础上于牙患处含化石辛含片,1片/次,4次/d。两组患者均治疗5 d。观察两组患者临床疗效,比较治疗前后两组OHIP-14和VAS评分以及菌群变化情况。结果治疗后,对照组和治疗组临床总有效率分别为83.75%、96.25%,两组比较差异具有统计学意义(P0.05)。治疗后,两组患者OHIP-14和VAS评分均显著降低,同组比较差异具有统计学意义(P0.05);且治疗组患者OHIP-14和VAS评分显著低于对照组,两组比较差异具有统计学意义(P0.05)。治疗后,两组细菌密度、格兰阴性菌比例、螺旋体比例均明显降低,而格兰阳性菌比例增加,同组比较差异具有统计学意义(P0.05);且治疗组菌群变化情况明显优于对照组,两组比较差异具有统计学意义(P0.05)。结论石辛含片联合碘甘油治疗智齿冠周炎可有效改善患者口腔健康,具有一定的临床推广应用价值。     

Iodinated curcumin bearing dermal cream augmented drug delivery,antimicrobial and antioxidant activities

Objectives: Curcumin (Cur) exhibits weak microbicidal activity owing to high lipophilicity and low cell permeability. Therefore, in the present investigation, Cur was iodinated using elemental iodine (I₂) to synthesise Cur–I₂ powder that was later formulated as Cur–I₂ dermal cream and characterised in vitro for antimicrobial and antioxidant activities.

Methods and results: Electrophilic addition of I₂ saturated the olefinic bonds of Cur, as confirmed by UV/visible spectroscopy, FT-IR, ¹H NMR and DSC techniques. In addition, in vitro skin permeation and retention analysis indicated that Cur–I₂ cream followed the first order and Higuchi model for drug release through the rat skin. The minimum inhibitory concentration (MIC) of Cur–I₂ powder was measured to be 60 and 90?µg/ml significantly (p?Staphylococcus aureus and Escherichia coli, respectively. Moreover, Cur–I₂ also exhibited strong antioxidant potential.

Conclusions: Cur–I₂ cream warrants further in vivo study to scale up the technology for clinical translation.     

Regulation of thyroid sodium‐iodide symporter in different stages of goiter: Possible involvement of reactive oxygen species

Na⁺/I^? symporter (NIS) transports iodide into thyrocytes, a fundamental step for thyroid hormone biosynthesis. Our aim was to evaluate NIS regulation in different status of goitrogenesis and its underlying mechanisms. Wistar rats were treated with methimazole (MMI) for 5 and 21 days, to achieve different status of goiter. We then evaluated the effect of MMI removal for 1 day (R1d), after 5 (R1d‐5d) or 21 (R1d‐21d) days of MMI treatment. MMI increased thyroid weight, iodide uptake and in vitro TPO activity in a time‐dependent way. Although MMI removal evoked a rapid normalization of TPO activity in R1d‐5d, it was still high in R1d‐21d. On the other hand, iodide uptake was rapidly down‐regulated in R1d‐21d, but not in R1d‐5d, suggesting that the increased TPO activity in R1d‐21d led to increased intraglandular organified iodine (I‐X), which is known to inhibit iodide uptake. Since TGFβ has been shown to mediate some effects of I‐X, we evaluated TGFβ and TGFβ receptor mRNA levels, which were increased in R1d‐21d. Moreover, it has been demonstrated that TGFβ stimulates NOX4. Accordingly, our data revealed increased NOX4 expression and H₂O₂ generation in R1d‐21d. Finally, we evaluated the effect of H₂O₂ on NIS function and mRNA levels in PCCL3 thyroid cell line, which were reduced. Thus, the present study suggests that there is a relationship between the size of the goiter and NIS regulation and that the mechanism might involve I‐X, TGFβ, NOX4 and increased ROS production.     

Investigating phase separation in amorphous solid dispersions via Raman mapping

The bioavailability of poorly-water-soluble active pharmaceutical ingredients (APIs) can be significantly improved by so-called amorphous solid dispersions (ASDs). However, the long-term stability of ASDs might be impaired by API recrystallization and/or amorphous phase separation (APS). So far, no methods have been reported to quantify APS in ASDs. In this work, phase-separation kinetics as well as the compositions of the two amorphous phases evolving due to APS were quantitatively determined for the first time using confocal Raman spectroscopy. Raman spectra were evaluated via non-linear multivariate Indirect Hard Modeling and verified by differential scanning calorimetry and hot-stage microscopy. APS in water-free ASDs of ibuprofen and poly (DL-lactic-co-glycolic acid) was investigated considering the influence of temperature and polymer architecture (linear vs. star-shaped). Water absorbed at 40?°C and 75% relative humidity (RH) promotes APS which was quantified for formulations of felodipine/poly(vinyl pyrrolidone) and ibuprofen/poly(vinyl pyrrolidone).     

葛根素联合卵磷脂络合碘片对眼底出血患者止血效果的影响

目的:探究葛根素联合卵磷脂络合碘片对眼底出血患者止血效果的影响。方法:选取2016-04/2017-03在我院就诊的眼底出血患者66例100眼,使用完全随机法将患者随机分为A组和B组,各33例50眼。A组给予卵磷脂络合碘片治疗,B组使用葛根素联合卵磷脂络合碘片治疗。两组均接受3个疗程的治疗。检测治疗前后血浆黏度指标、血清同型半胱氨酸(Hcy)、总胆固醇(TC)和甘油三酯(TG)水平变化,统计两组患者眼底出血治疗效果和不良反应发生率。结果:治疗前两组患者血浆黏度、血清Hcy、TC和TG指标无差异(P>0.05)。治疗后,两组患者的全血黏度高切、全血黏度低切、血浆黏度、Hcy、TC和TG均低于治疗前(P<0.001);与A组比较,B组患者全血黏度高切、全血黏度低切、血浆黏度、Hcy、TC和TG均降低(P<0.05)。B组患者较A组治疗总有效率增高(P<0.05)。两组患者均发生不同程度的甲状腺变化、胃肠道反应、精神兴奋和腹胀,两组患者不良反应生率无差异(P>0.05)。结论:葛根素联合卵磷脂络合碘片治疗眼底出血患者疗效显著,与改善患者血浆黏度、降低血清Hcy、TC和TG有关。     

江西省第6次碘缺乏病病情调查结果分析

目的通过调查全省碘缺乏病病情和居民碘营养水平,为调整江西省食盐加碘浓度提供依据。方法按人口比例概率抽样方法在全省抽取30个县（市、区）的30所小学．每所小学采用B超法测定40名学生甲状腺容积．抽取上述小学8～10岁儿童尿样345份,并在每所学校周边的3个乡（镇）分别采集孕妇和哺乳期妇女尿样共894份．采用砷铈催化分光光度法进行尿碘含量检测,并对学校所在村分别采集饮用水进行水碘含量检测。结果监测结果表明．全省儿童学生甲状腺肿大发生率为1．25％;盐碘中位数为30．80mg／kg,居民合格碘盐食用率为97．00％;儿童、孕妇和哺乳期妇女尿碘中位数分别为308．95、206．75、206．72μgg/L,其中儿童尿碘大于或等于300μg／L的样品数占52．20％,而孕妇尿碘小于150μg/L的样品数占34．73％;全省水碘中位数4．62μg/L。结论江西省人群碘缺乏状况总体控制良好,仍要加强对重点人群碘营养水平的监测,适时调整碘盐浓度。     

2011年赤峰市8~10岁儿童尿碘监测结果分析

目的了解赤峰市8~10岁儿童碘营养水平现状,为赤峰市的碘缺乏病防治工作提供科学依据。方法在赤峰市按照随机抽样原则,对8~10岁儿童的尿碘浓度进行监测。结果在7个旗(县区)共采取了8~10岁儿童的尿样711份进行监测,尿碘浓度中位数为268.92μg/L,并且7个旗县区的尿碘中位数都200μg/L,并且有3个旗(县区)的尿碘中位数超过了300μg/L处于一个碘过量的水平。结论 7个旗县区儿童尿碘水平均达到了国家要求。建议在赤峰市进一步研究碘摄入量增加对甲状腺疾病的影响,并根据本市各地区碘营养状况适当调整食用盐中碘的含量,以使居民的碘摄入量在适宜的范围内。     

聚维酮碘稀释液腹腔冲洗在预防坏疽性阑尾炎伴穿孔术后腹腔及切口感染中的作用

目的探讨聚维酮碘稀释液腹腔冲洗在预防坏疽性阑尾炎伴穿孔术后腹腔及切口感染的临床效果。方法选择我院近年来手术治疗的坏疽性阑尾炎伴穿孔患者为研究对象,术中分别应用聚维酮碘稀释液、甲硝唑和生理盐水对腹腔及切口进行冲洗,对比分析三组患者术后腹腔及切口感染情况、体温恢复正常时间、术后住院时间以及相关不良反应发生情况。结果聚维酮碘组和甲硝唑组腹腔及切口感染发生率比较差异无统计意义(P>0.05),且均明显低于生理盐水组,差异有统计学意义(P<0.05);三组患者体温恢复正常平均时间比较差异无统计学意义(P>0.05);聚维酮碘组和甲硝唑组患者平均住院时间均明显短于生理盐水组(P<0.01),而聚维酮碘组和甲硝唑组比较差异无统计学意义(P>0.05);甲硝唑组术后不良反应发生率高于聚维酮碘组和生理盐水组,但差异均无统计学意义(P>0.05)。结论聚维酮碘稀释液腹腔冲洗能有效降低坏疽性阑尾炎伴穿孔术后腹腔及切口感染发生率,缩短住院时间,且操作简单,不良反应少,值得临床推广应用。     

Antioxidant effects of vitamin D on lacrimal glands against high dose radioiodine-associated damage in an animal model

Purpose: To evaluate antioxidant effects of active vitamin D (calcitriol) against high-dose radioiodine (RAI) therapy-associated damage of lacrimal gland.

Materials and methods: Wistar albino rats were used and divided into three groups randomly (n?=?12/group). The first group was appointed as the negative control group and received no RAI or medication. The second group was appointed as the positive control group that only received 3?mCi/kg (111 MBq/kg) RAI via gastric gavage and the last group was the treatment group that received 3?mCi/kg RAI via same method and calcitriol (200?ng/kg/day) via intraperitoneal administration. Seven days after RAI administration, bilateral intraorbital (IG), extraorbital (EG) and Harderian (HG) glands were removed for the evaluations of histopathologic, tissue cytokine, total oxidant status (TOS) and total antioxidant status (TAS).

Results: RAI led to significant increase in tissue TOS, TNF-α, IL-6 levels and significant decrease in IL-10 and TAS levels (p?p?p?p?=?0.049), periductal fibrosis in EG and HG (p?=?0.049 and 0.038, respectively), abnormal cell outlines in EG and HG (p?=?0.020 and 0.011, respectively) and variation in cell size in the IG and the HG (p?=?0.003 and 0.049 respectively).

Conclusions: RAI caused significant oxidative stress and inflammation in lacrimal glands. Vitamin D demonstrated potent anti-inflammatory, antioxidant and radio-protective effects on lacrimal glands in histopathologic, tissue cytokine and oxidant/antioxidant level evaluations.