接种COVID-19疫苗后罹患带状疱疹 —附三例报告

报道3例接种COVID-19疫苗后发生带状疱疹的患者,二者之间的因果关系尚待确定。     

生殖器疱疹HSV定量PCR检测及分析

目的:检测生殖器疱疹(GH)皮损的HSV含量并分析其与复发的关系。方法:用定量PCR技术检测96例临床诊断为GH的患者皮损标本中的HSV量。结果:HSV的阳性率为87.5％,男女性病例之间(2.7×109拷贝对2.1×108拷贝)、初发病例与复发病例之间(4.5×109拷贝对2.3×108拷贝),检出拷贝量的差别没有显著性意义(P>0.05),GH病人发作间歇期长短与检出的HSV拷贝量呈负相关(r=-0.728,P<0.01),病人病期、发疹天数、皮损数目与HSV拷贝量无明显相关关系(P值均大于0.05)。结论:通过定量PCR检测GH病人皮损中的HSV含量,可帮助判断预后并指导治疗。     

Recalcitrant hypertrophic herpes genitalis in HIV‐infected patient successfully treated with topical imiquimod

Herpes Simplex Virus type 2 is the primary cause of genital ulceration worldwide. The presence of atypical features like deep ulcerations, hypertrophic, or pseudotumoural lesions or unusual location can be a marker for co‐infection with HIV. These immunocompromised patients are usually resistant to the conventional antiviral treatment. We present a case of an HIV‐infected patient with hypertrophic herpes genitalis, refractory to conventional oral antiviral therapy, who was successfully treated with a combination of oral valcyclovir and topical application of 5% imiquimod.     

带状疱疹患者外周血T细胞及NK细胞检测

目的:评价机体免疫功能与带状疱疹的相关性。方法:选取25例带状疱疹患者和30名对照者,流式细胞术检测CD4~+T细胞、CD3~+T细胞、CD8~+T细胞、CD4/CD8及NK细胞。结果:与对照组相比较,患病组CD4~+T细胞、CD3~+T细胞降低,NK细胞升高。差异有统计学意义(P0.05)。结论:带状疱疹存在免疫功能异常。     

带状疱疹相关性疼痛机制及治疗研究进展

带状疱疹相关性疼痛贯穿带状疱疹发病全程,既是患者就医原因,也是医者关注重点.如何早期介入治疗、减少后遗神经痛、提高生活质量,一直是临床治疗的关键.带状疱疹相关性疼痛临床表现多样,发病机制复杂,治疗方案多样尚无明确统一的临床指南.本文就治疗方面新进展作一综述,以期为后续临床治疗方案优化提供线索及理论支持.     

The Role of Herpes simplex and Helicobacter pylori Infection in the Etiology of Persistent or Recurrent Gastric Erosions: A Follow-Up Study

The etiology of chronic gastric erosions is unknown. We have evaluated the significance of Helicobacter pylori and herpes simplex virus (HSV) infections, the use of nonsteroidal antiinflammatory drugs (NSAIDs), alcohol, and smoking in a prospective long term follow-up study. A prospective series of 117 patients with gastric erosions and 117 controls were studied in 1974–1981, and invited for reendoscopy in 1996. At both visits, H. pylori infection was diagnosed by histology, serum HSV antibodies were measured, and the use of NSAIDs, alcohol, and smoking was evaluated by interview. Biopsies from erosions from the latter visit were studied for HSV by immunohistochemistry and polymerase chain reaction (PCR). In the follow-up visit, 16 of 42 patients had still gastric erosions while six of 47 controls had developed erosions. No HSV antigen or DNA could be detected in biopsy specimens. However, only high antibody titers (32) against HSV at the first visit predicted persistence of erosions (P = 0.000), while H. pylori infection, use of NSAIDs, alcohol, or smoking were not associated with chronic erosions. High HSV titers at the follow-up visit were also significantly associated with concurrent erosions in the patient group. In conclusion, the results suggest that a significant proportion of chronic gastric erosions are related to HSV infection.     

Synthetic radiation‐inducible promoters mediated HSV‐TK/GCV gene therapy in the treatment of oral squamous cell carcinoma

Oral Diseases (2010) 16 , 445–452 Objective: To investigate the therapeutic effect of herpes simplex virus thymidine kinase (HSV‐TK) gene mediated by synthetic radiation‐inducible promoters in the treatment of oral squamous cell carcinoma (OSCC) in vitro and in vivo . Methods: The plasmids pcDNA3.1(+)E6‐HSV‐TK were constructed, in which the HSV‐TK genes were mediated by synthetic radiation‐inducible promoters. The recombined plasmids were transfected into the Tca8113 cells and golden hamster buccal carcinoma, respectively. Low‐dose radiotherapy was used to upregulate the HSV‐TK genes expression. HSV‐TK mRNA was assayed by RT‐PCR. Apoptosis and proliferating cell nuclear antigen were detected respectively by in situ end‐labeling and immunohistochemical method. Results: Compared with control group, the comparative survival rate of Tca8113 cells in HSV‐TK/GCV/IR group was markedly decreased and the golden hamster buccal carcinoma in HSV‐TK/GCV/IR group was obviously suppressed. Up‐regulation of HSV‐TK gene expression was found in the Tca8113 cells and in the golden hamster buccal carcinoma resulting from exposure to low‐dose irradiation. The apoptosis indexes in Tca8113 cells or golden hamster buccal carcinoma with irradiation were markedly higher than those without irradiation. At the same time, the proliferation indexes in Tca8113 cells or golden hamster buccal carcinoma with irradiation were markedly lower than those without irradiation. Conclusion: The results indicate that the synthetic radiation‐inducible promoters can serve as a molecular switch to adjust the expression of HSV‐TK gene in the treatment of OSCC, and low‐dose induction radiation can significantly improve therapeutic efficiency.     

Prevalence of infection with herpes simplex virus types 1 and 2 in Australia: a nationwide population based survey

BACKGROUND: Studies demonstrating previous herpes simplex virus (HSV) type 2 infection as a risk factor for HIV transmission, and the development of a HSV vaccine candidate, have emphasised the need for worldwide population based studies of HSV seroprevalence. The only nationwide seroprevalence studies have been conducted in the United States. METHODS: An Australia-wide, population based study of HSV-1 and HSV-2 seroprevalence was conducted, using serum and sociodemographic data collected between 1999-2000, for a representative study of risk factors for diabetes in over 11 000 adults. A stratified random sample of 4000 was tested for HSV-2 and 1000 for HSV-1, with sampling and weighting for various demographic factors. RESULTS: Seroprevalence of HSV-2 in Australian adults was 12%. Prevalence in women (16%) was twice that in men (8%). Rural populations had a lower prevalence (9%) than metropolitan (13%), and Indigenous had a higher prevalence (18%) than the non-Indigenous populations (12%). The seroprevalence of HSV-1 was 76% with significant differences by age group, sex and Indigenous status. CONCLUSION: These are the first nationwide data to compare with US studies. HSV-2 infection is less common in Australia than the United States, and this will allow planning for combating HIV transmission in high prevalence populations in northern Australia. In addition, the high HSV-1 seroprevalence will be important for future deployment of genital herpes vaccines.