食管鳞癌患者组织和血清中VEGF-C的表达

目的:检测食管鳞癌患者组织和血清中VEGF-C的表达,探讨其在判断淋巴结转移中的作用.方法:62例食管鳞癌患者和10例正常人的血清,用酶联免疫吸附法检测血清中VEGF-C的含量,用免疫组织化学法检测食管癌组织中VEGF-C的表达,并进行ROC曲线分析.结果:食管鳞癌组织中VEGF-C在有淋巴结转移者的阳性表达率高于无转移者(P=0.015),肿瘤浸润深度T3者阳性表达率高于T1-2者(P=0.039);组织学检查诊断淋巴结转移的灵敏度为55.5%,特异性为76.1%,准确率为67.8%.食管鳞癌患者血清VEGF-C的水平高于正常人(P＜0.0001),有淋巴结转移者高于无转移者(P＜0.0001),肿瘤浸润深度T3者高于T1-2者(P=0.009);如果血清VEGF-C水平以2 474.5 ng/L为界值,则诊断淋巴结转移的灵敏度和特异性分别为85.0%和73.8%,准确率77.4%.结论:血清VEGF-C检测是判断食管鳞癌淋巴结转移的可靠标志物.     

角膜后弹力膜内皮移植术研究进展

角膜后弹力膜内皮移植术由于具有相对较低的移植排斥率以及较好的视力预后等优势,目前已成为部分发达国家治疗角膜内皮失代偿的主流手术方式,但限于手术难度较高,学习曲线较长,中国人前房偏浅,加之国内角膜内皮病变往往合并有其他较复杂的眼部疾病,目前国内尚未普遍开展这一手术。本文就角膜后弹力膜内皮移植术的手术适应证、供体植片制备(...     

Protective effects of beet (Beta vulgaris) leaves extract against oxidative stress in endothelial cells in vitro

Beetroot is an herb used worldwide as a food product, raw material for food industry, ethanol production and source of food coloring. Beet leaves are an unconventional food with antioxidant properties, which might neutralize reactive oxygen species (ROS) induced by oxidized Low‐Density Lipoprotein (LDL) present in dyslipidemias. This study aimed to elucidate the effects of beet leaves on the suppression of LDL oxidative processes. Beet leaves extract was produced, characterized, and tested for its antioxidant capacity using endothelial cells in vitro. A model of human umbilical vein endothelial cells was used in various tests, including viability assay, molecular analysis of antioxidant genes, ROS labeling, and macrophage adhesion assay. The extract improved the antioxidative protection of endothelial cells against different agents including oxidized LDL‐cholesterol and H₂O₂. It acted on ROS directly due to its high content of natural antioxidants, but also due to the activation and improvement of cellular defenses such as Superoxide dismutase 1, Superoxide dismutase 2, and catalase. The inhibition of LDL‐mediated oxidative effects on endothelial cells may turn this unconventional food a functional food with great potential for phytotherapy of atherosclerosis as an adjuvant for medicinal treatments.     

滋肾续骨汤联合鲑鱼降钙素对老年椎体压缩性骨折术后血清骨形态发生蛋白9及血管内皮生长因子水平的影响

目的观察滋肾续骨汤联合鲑鱼降钙素对老年椎体压缩性骨折术后血清骨形态发生蛋白9(BMP9)及血管内皮生长因子(VEGF)水平的影响。方法将80例老年椎体压缩性骨折术后患者按照随机数字表法分为2组。对照组39例予鲑鱼降钙素治疗;治疗组41例在对照组治疗基础上加用滋肾续骨汤治疗。2组均治疗24周后统计临床疗效,并观察2组治疗前后血清BMP9、VEGF水平及疼痛视觉模拟评分(VAS)、胸腰椎损伤分类及损伤程度评分系统(TLICS)、Oswestry功能障碍指数(ODI)评分、日本骨科协会(JOA)腰痛评分,以及椎体前缘丢失高度、伤椎Cobb角、伤椎骨密度水平及并发症发生情况。结果治疗组总有效率95.12%(39/41),对照组总有效率76.92%(30/39),治疗组临床疗效优于对照组(P<0.05)。2组治疗后血清BMP9、VEGF水平均升高(P<0.05),且治疗组高于对照组(P<0.05)。2组治疗后疼痛VAS、TLICS评分均降低(P<0.05),且治疗组低于对照组(P<0.05)。治疗后2组ODI评分均降低(P<0.05),且治疗组低于对照组(P<0.05);治疗后2组JOA腰痛评分均升高(P<0.05),且治疗组高于对照组(P<0.05)。治疗后2组椎体前缘丢失高度、伤椎Cobb角均降低(P<0.05),伤椎骨密度均升高(P<0.05),且治疗组改善均优于对照组(P<0.05)。治疗组并发症总发生率4.88%(2/41),对照组并发症总发生率20.51%(8/39),治疗组并发症总发生率低于对照组(P<0.05)。结论滋肾续骨汤治疗老年椎体压缩性骨折术后患者疗效显著,可有效改善临床症状及血清BMP9、VEGF水平。     

松弛素保护心肌微血管内皮细胞缺氧复氧损伤的机制

背景：研究报道松弛素可显著改善病理因素导致的心肾功能障碍,抑制心肌肥厚、抗纤维化以及改善缺血再灌注损伤等,但其对内皮细胞缺氧复氧损伤的保护机制尚不明确。目的：探讨松弛素保护心肌微血管内皮细胞缺氧复氧损伤的机制。方法：取生长状态良好的小鼠心肌微血管内皮细胞系(H5V),分3组处理：对照组常规培养33 h,模型组常规培养24 h后给予6 h缺氧+3 h复氧模拟心肌缺氧再灌注损伤,松弛素组常规培养(加180 ng/mL松弛素)24 h后给予6 h缺氧+3 h复氧(加180 ng/mL松弛素)模拟心肌缺氧再灌注损伤。检测细胞通透性与Caspase-3的表达,细胞上清中肿瘤坏死因子α、白细胞介素1β和白细胞介素6水平,RT-PCR检测细胞中钙黏蛋白、Akt1、GSK-3β mRNA表达;Western blot检测细胞中钙黏蛋白、Akt、GSK-3β蛋白表达。结果与结论：(1)与对照组比较,模型组细胞通透性增强(P <0.05),细胞内Caspase-3表达升高(P <0.05);与模型组比较,松弛素组细胞通透性降低(P <0.05),细胞内Caspase-3表达降低(P &l...     

子宫内膜异位症患者治疗前后血清TSGF和血浆VEGF水平的临床意义

目的：探讨了子宫内膜异位症患者治疗前后血清TSGF和血浆VEGF水平的变化及临床意义。方法：应用ELISA法测定31例子宫内膜异位症患者血浆VEGF含量,光电比色法测定TSGF含量,并与30名正常健康人作比较。结果：子宫内膜异位症患者在治疗前血清TSGF和血浆VEGF水平非常显著地高于正常人组（P＞0．01）。经中西医结合治疗后3个月,则与正常人组比较无显著性差异（P〉0．05）。结论：子宫内膜异位症的发生与发展与血清TSGF和血浆VEGF水平密切相关。     

艾灸通过成纤维细胞生长因子受体1和血管内皮细胞生长因子受体2抑制肉瘤微环境血管生成

目的:观察艾灸对肉瘤微环境中成纤维细胞生长因子受体1(FGFR1)和血管内皮细胞生长因子受体2(VEGFR2)的影响,探讨艾灸防治肉瘤的作用机制.方法:将接种S180肉瘤细胞形成移植瘤的C57BL/6J小鼠分为模型组、顺铂组和艾灸组,每组10只.艾灸组直接灸移植瘤,距离3 cm,10 min/次,每日1次,治疗14 d...     

Molecular Mechanisms Involved in Intrarenal Renin-Angiotensin and Alternative Pathways in Diabetic Nephropathy - A Review

Uncontrolled or chronic hyperglycemia causes kidney failure induced by the dysfunction of biomolecules and upregulation of inflammatory cytokines and growth factors. The reninangiotensin system (RAS) is incorporated in the regulation of renal hemodynamics. In a healthy state, local RAS is independent of systemic RAS. However, in pathological conditions such as chronic hyperglycemia, angiotensin II (Ang II) increases locally and causes tissue damage, mainly through the induction of oxidative stress, inflammation, and upregulation of some growth factors and their receptors. Such tissue events may cause disruption of the glomerular filtration barrier, thickening and hypertrophy of the glomerular basement membrane, microvascular hyperpermeability, proteinuria, and finally decrease in the glomerular filtration rate (GFR). Reduced GFR causes the kidney to sense falsely a low blood pressure condition and respond to it by stimulating systemic and local RAS. Therefore, patients with diabetic nephropathy (DN) suffer from chronic hypertension. In contrast to local RAS, there are alternative pathways in the kidney that act protectively by reducing tissue Ang II. Such autoregulatory and protective mechanisms are weakened in chronic kidney disease. Previously, it was presumed that systemic RAS inhibitors such as ACE inhibitors (ACEIs) or angiotensin receptor blockers (ARBs) could prevent renal damage by controlling blood pressure and proteinuria. However, the progression of renal failure to end-stage renal disease (ESRD), despite such treatments, indicates the presence of factors other than Ang II. This review highlights the molecular mechanism in renal disease and discusses pharmaceutical and therapeutic approaches.     

中药天然产物调控内皮细胞防治动脉粥样硬化的研究进展

动脉粥样硬化是冠心病发病的病理基础,而血管内皮损伤是冠状动脉粥样硬化的始动因素。血管内皮细胞是位于血管内层的单层细胞,调控血液和周围组织的物质交换,其完整性十分重要。中药天然产物活性单体及其衍生物通过抑制氧化应激、调控血管活性物质释放、减轻机体炎症、平衡凝血与抗凝血系统等途径调控内皮细胞功能,在内皮保护和动脉粥样硬化防治方面具有多途径、多环节、多靶点的优势,但也有报道有关中药天然产物抵抗新型冠状病毒所致内皮损伤和动脉粥样硬化作用的研究。本文对该领域的研究进展进行综述。     

血管内皮细胞生长因子、碱性成纤维细胞生长因子在正常成人皮肤中的表达及其意义

目的探讨两种促血管生成因子血管内皮细胞生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)在正常成人皮肤组织中的表达及其意义.方法采用免疫组化SP法检测20例正常成人皮肤组织VEGF、bFGF的表达及分布.结果正常成人皮肤组织中,VEGF、bFGF阳性细胞均主要位于基底层,呈弱阳性表达.结论正常成人皮肤组织中,基底层是表达VEGF、bFGF的主要细胞,在维持真皮微血管的正常生理功能中可能发挥重要作用.