Analysis of collagen fibres in human dental pulp using picrosirius red stain and polarised microscopy

BackgroundThe extracellular matrix (ECM) performs a very important function in growth regulation, tissue differentiation and organization. Diseases of pulp are seen in all ages. Collagen, the main macromolecule of dental pulp, varies in its number, thickness and distribution with age. Hence a study was designed to identify and analyze the collagen fibers in human dental pulp on the basis of polarizing colors in various age groups.Material and methodsA study was conducted using the pulp of 30 non-carious teeth from patients of various ages and one tooth germ. Picrosirius red stain was used to stain the collagen present in dental papilla, dental follicle and dental pulp and the thickness of the fibers was assessed on the basis of the polarizing colors.ResultsCollagen fibers in dental follicle stained red while they were not stained in papilla. Collagen fibers in the pulp of teeth of younger individuals stained green to greenish yellow, in middle aged individuals, it stained orange–orange red while in older individuals, it stained red.ConclusionThe results from the study show that with aging, thickness of collagen fibers increases and there is change in their arrangement and packaging indicating fibrosis.     

短葶飞蓬总黄酮含量变化研究

目的 研究短葶飞蓬总黄酮含量与生长发育和月份变化的关系，环境与遗传因素对总黄酮含量的决定作用。方法 测定同一月份不同生长发育期、同一生长发育期不同月份短葶飞蓬的总黄酮含量的器官分布，阳生和阴生生境下短葶飞蓬的总黄酮含量，不同花色短葶飞蓬的总黄酮含量。结果 短葶飞蓬的总黄酮主要分布于地上部分，而地上部分的总黄酮含量以盛花期员高，谢花期最低。4～6月盛花期短葶飞蓬各器官的总黄酮含量月份变化不显著。光照强度对短葶飞蓬总黄酮含量影响不大。短葶飞蓬的花色不能作为总黄酮含量的选择标记，遗传因素对个体间总黄酮含量差异起重要作用。结论 通过遗传育种提高短葶飞蓬总黄酮含量的前景是广阔的。     

糖果中6种合成色素液相色谱检测方法的改进

目的:对糖果中6种合成色素(柠檬黄、苋菜红、胭脂红、日落黄、诱惑红、亮蓝)的高效液相色谱检测方法进行改进。方法:样液经聚酰胺粉吸附,用氨水-乙醇溶液解吸附,用乙酸中和后浓缩,定容过滤。以甲醇和乙酸胺为流动相,在C18色谱柱上分离,梯度洗脱,VWD和DAD检测器串联,紫外254 nm及可见600 nm检测,外标法定量。结果:改进样品前处理方法,改变亮蓝的检测波长,亮蓝的响应值显著增加,加标回收实验中,6种混合色素的平均回收率在90%以上。结论:检测过程中控制每个步骤的实验条件,梯度洗脱,变波长检测,亮蓝的灵敏度显著提高,各色素的分离度良好,回收率满意。     

Pruritic and antipruritic colors: An exploratory pilot study

Itch is the commonest skin‐related symptom and can be influenced by visual cues as exemplified by the phenomenon of “contagious itch.” Colors are visual cues able to modify somatosensory inputs. We explored the relationship of colors and itch and the impact of color viewing on itch intensity. In this cross‐sectional study, patients suffering from itch with a mean intensity of ≥2 on a Numerical Rating Scale during the last 7 days were evaluated. The study consisted of a questionnaire‐based part using The Manchester Color Wheel and the ItchyQoL, followed by an interventional part. All 72 itch patients were able to match their itchy sensation with a color: In 68 patients (94.4%) this “pruritic” basic color was red. Likewise, all patients were able to define a subjective “antipruritic” color: The leading basic color choice was blue (31/72, 43.0%) followed by green (21/72, 29.1%), yellow (7/72,9.7%) and others. The impairment of the itch‐related quality of life (as measured by the ItchyQoL) correlated with the brightness and saturation of the pruritic and antipruritic colors. Ten patients were visually exposed to their subjective antipruritic and pruritic color during 10 minutes resulting in a significant decrease and increase of itch intensity compared to baseline (5.1 ± 1.52 vs. 2.8 ± 1.47 [0‐10 Numerical Rating Scale, NRS], p=0.0004 and 4.9 ± 1.66 vs. 6.8± 2.09 NRS, p=0.0009). These results indicate that itch can be modified by color viewing and colors matter when treating itch patients. However, further investigations are required to elucidate the therapeutic potential of colors in itch patients.     

数字舌图的色彩校正方法研究

在舌诊客观化的研究中，舌色的真实还原是一个关键的技术环节。本文运用ICC色彩特性文件对数字图像进行了色彩校正，实验结果表明，此方法能够有效的校正数字图像的色偏，是一种行之有效的色彩还原方法。     

Nutritional values for crop diversification matrix

Previously published crop diversification matrices scored 1000 crops species for their tolerances to annual temperature, annual precipitation, pH, and frost‐free‐drought‐free months. These same 1000 species have been classified into four categories each for 16 nutrients. The matrix system enables one to determine rapidly which crops having particular combinations of nutritional parameters will tolerate special ecological situations.     

Structural Color and Iridescence in Transparent Sheared Cellulosic Films

Shear transparent cellulose free‐standing thin films can develop iridescence similar to that found in petals of the tulip “Queen of the Night.” The iridescence of the film arises from the modulation of the surface into bands periodically spread perpendicular to the shear direction. Small amounts of nanocrystalline cellulose (NCC) rods in the precursor liquid‐crystalline solutions do not disturb the optical properties of the solutions but enhance the mechanical characteristics of the films and affects their iridescence. Smaller bands periodicity, not affected by the NCC rods, slightly deviated from the shear direction is also observed. NCCs are crucial to tune and understand the film's surface features formation. Our findings could lead to new materials for application in soft reflective screens and devices.     

HPLC法测定明胶空心胶囊中的人工合成色素

目的 建立RP-HPLC法测定明胶空心胶囊中7种人工合成色素的方法.方法 样品溶液经聚酰胺吸附,用氨-乙醇-水溶液解吸附,中和后水浴浓缩,定容过滤,采用SunFire C18柱,以0.02 mol·L-1醋酸铵溶液-甲醇为流动相,梯度洗脱,流速为1.0mL·min-1,检测波长254 nm和627 nm,柱温为25℃,进样量为10 μL.结果 在选定的色谱条件下,柠檬黄、苋菜红、胭脂红、诱惑红、日落黄、亮蓝、赤藓红7种色素均可得到良好的分离;7种色素在10.0～100.0 μg· mL-1范围内均能呈较好的线性关系;平均回收率(n=9)均在90％以上.利用该方法对40批明胶空心胶囊中的色素进行测定.结论 该方法准确、可靠,可用于明胶空心胶囊中色素的检测.     

糖尿病患者后像消近试验的临床研究

目的　研究后像消逝试验与 DR早期诊断的相关性。方法　对 NIDDM者 75眼、正常对照 46眼分别检测后消逝试验、FFA及 30°范围视网膜光敏度。结果　正常对照组的 TFC与 NDR组、DR组比较 ,P <0 .0 1,差异非常显著。 NIDDM者中 ,视网膜光敏度正常组与下降组比较 TFC,P <0 .0 1,差异非常显著。 NDR者中 ,病程≤5年组与 >5年组的 TFC比较 ,P <0 .0 1,差异非常显著。结论　后像消逝试验可作为 DR早期诊断和判断预后的重要参考指标     

188Re-抗CEA单抗治疗小鼠结肠癌模型的疗效观察

目的 观察^188Re抗CEA单抗（C50）对小鼠结肠癌模型的治疗作用，评估不同给药方法对疗效的影响。方法 将BALB／C小鼠结肠癌模型随机分成5组，分别为对照组、化疗组、RIT1组、RIT2组、RIT3组，用不同的处理方法治疗3周后将小鼠处死，取出肿瘤，测量肿瘤体积与重量，分析不同处理方法对肿瘤生长的影响。结果 通过不同的治疗3周后肿瘤体积、重量的比较可以看出，RIT各组与对照组之间均存在明显的差异，其中肿瘤生长受抑制最明显的是RIT3组（瘤内注射18.5MB/只），3周后肿瘤平均体积为1043.54mm^3，平均重量为3.2378g，瘤内注射组（RIT3组、RIT2组）与静脉给药组（RIT1组）及RIT局部注射组与化疗组之间也存在明显的差异（P＜0．05）。结论 ^188Re-C50对小鼠结肠癌模型的治疗有效，疗效优于5－Fu化疗，瘤内注射给药的疗效优于静脉给药，为^188Re-C50应用于临床治疗结肠癌提供了可靠的依据。