修复产品联合应用在点阵铒激光术后创面修复中的评价

目的:评价修复产品联合应用在点阵铒激光治疗后创面修复中的作用。方法:纳入面部行点阵铒激光治疗的受试者15例,采用自身对照策略,试验侧治疗后给予含有PDGF、KGF、积雪草苷成分的修复产品,包含水剂、面贴膜和霜剂,持续一周,对照侧治疗后空白对照,观察治疗后创面修复情况及不良反应。结果:与对照侧比,修复产品联合应用后,患者创面红肿及脱痂时间明显缩短,肿痛、紧绷感减少,没有不良反应发生。结论:修复产品联合应用对点阵铒激光治疗后创面修复有安全、有效的促进作用。     

蛇床子挥发油纳米粒在双骨架复合凝胶基质中的稳定性及其对积雪草总苷透黏膜吸收的影响

目的 考察复方葡配甘露聚糖水凝胶（专利号：ZL202010787982.9）处方中蛇床子挥发油纳米粒（Cnidii Fructus volatile oil nano-particulates,CVOP）在双骨架复合凝胶基质中的稳定性,以及CVOP对处方中积雪草总苷透黏膜吸收的影响。方法 通过冷冻电子显微镜（cryo-electron microsocopy,Cryo-EM）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）对凝胶基质结构以及其中的CVOP进行观察,以CVOP的外观形态、粒径为评价指标,结合GS-MS法,以CVOP的组成成分谱图及含量变化为判断依据,评价CVOP的稳定性;采用体外透黏膜试验考察CVOP对处方中积雪草总苷透阴道黏膜吸收的影响。结果 蛇床子挥发油（Cnidii Fructus volatile oil,CO）在双骨架复合凝胶基质中能够形成圆整的球形粒子结构,平均粒径为（185±26）nm;CVOP在凝胶基质中稳定性良好,6个月加速试验后其形态结构、粒径与组成成分基本不变;CVOP对大鼠阴道黏膜不产生刺激性;在不含CVOP的空白水凝胶（B）中,积雪草苷（asiaticoside,AS）和羟基积雪草苷（madecassoside,MAS）的黏膜渗透性始终低于含有CVOP的水凝胶（A）组;在6.0 h时,水凝胶A中AS和MAS的累积渗透量分别是水凝胶B中AS和MAS的4.68倍与2.01倍,表明CVOP能够提高水溶性积雪草总苷的阴道黏膜渗透性。结论 CO在双骨架复合凝胶基质中能够形成稳定的球形纳米粒,并可提高积雪草总苷的黏膜渗透性而有利于发挥多成分间的协同治疗作用。     

Improving lip wrinkles: lipstick-related image analysis

BACKGROUND/PURPOSE: The appearance of lip wrinkles is problematic if it is adversely influenced by lipstick make-up causing incomplete color tone, spread phenomenon and pigment remnants. It is mandatory to develop an objective assessment method for lip wrinkle status by which the potential of wrinkle-improving products to lips can be screened. The present study is aimed at finding out the useful parameters from the image analysis of lip wrinkles that is affected by lipstick application. METHODS: The digital photograph image of lips before and after lipstick application was assessed from 20 female volunteers. Color tone was measured by Hue, Saturation and Intensity parameters, and time-related pigment spread was calculated by the area over vermilion border by image-analysis software (Image-Pro). The efficacy of wrinkle-improving lipstick containing asiaticoside was evaluated from 50 women by using subjective and objective methods including image analysis in a double-blind placebo-controlled fashion. RESULTS: The color tone and spread phenomenon after lipstick make-up were remarkably affected by lip wrinkles. The level of standard deviation by saturation value of image-analysis software was revealed as a good parameter for lip wrinkles. By using the lipstick containing asiaticoside for 8 weeks, the change of visual grading scores and replica analysis indicated the wrinkle-improving effect. As the depth and number of wrinkles were reduced, the lipstick make-up appearance by image analysis also improved significantly. CONCLUSION: The lip wrinkle pattern together with lipstick make-up can be evaluated by the image-analysis system in addition to traditional assessment methods. Thus, this evaluation system is expected to test the efficacy of wrinkle-reducing lipstick that was not described in previous dermatologic clinical studies.     

积雪草苷修饰脂质体在大鼠体内的药动学及组织分布研究

目的将积雪草苷制成修饰脂质体,考察该制剂在体内的药动学过程及组织分布情况。方法 SD大鼠尾iv积雪草苷溶液及经过D-甘露糖或十八胺修饰和未经修饰的脂质体制剂,不同时间点采集大鼠血浆及组织,样品处理后采用HPLC法检测血浆与组织中的药物浓度,绘制药-时曲线,计算药动学参数,比较积雪草苷剂型间在体内药动学参数和组织分布的差异。结果积雪草苷、积雪草苷脂质体、D-甘露糖-积雪草苷脂质体、十八胺-积雪草苷脂质体4种制剂的药-时曲线均符合权重为1/C2的单室模型。积雪草苷溶液及各脂质体的消除半衰期(t1/2)分别为(14.52±0.56)、(101.35±12.47)、(149.82±20.00)、(159.58±16.46)min,药时曲线下面积(AUC)分别为(1 929.70±159.00)、(57 004.35±8 710.89)、(93 736.52±12 710.76)、(64 737.48±6 365.28)min·μg/m L。同时制成脂质体后,积雪草苷在各器官中的质量分数显著上升,在肺部的质量分数由(4.94±0.94)μg/g升至(39.12±12.04)μg/g。结论将积雪草苷制成修饰脂质体制剂后,缓释明显,并能增强靶向作用。     

高效液相色谱法测定积雪苷片中积雪草苷和羟基积雪草苷的含量

目的：建立积雪苷片中积雪草苷和羟基积雪草苷含量的测定方法。方法：采用C18柱,RP—HPLC法,乙腈-甲醇-水（20：20：60）为流动相,流速为1．0mL／min,检测波长为204nm。结果：通过方法学考察,积雪草苷进样量在2．088μg～10.44μg内线性关系良好,加样回收率为97．5％,RSD为1．73％。羟基积雪草苷进样量在2．078μg-10．39μg内线性关系良好,加样回收率为98．2％,RSD为1．22％。结论：高效液相色谱法简便、准确、重复性好,所建立的积雪草苷和羟基积雪草苷含量测定方法可以控制本品的质量。     

Matrix metalloproteinase,hyaluronidase and elastase inhibitory potential of standardized extract of Centella asiatica

Abstract

Context: Centella asiatica (L.) Urban (Apiaceae), a valuable herb described in Ayurveda, is used in the indigenous system of medicine as a tonic to treat skin diseases.

Objective: Centella asiatica methanol extract and its ethyl acetate, n-butanol and aqueous fraction, were subjected for the evaluation of skin care potential through the in vitro hyaluronidase, elastase and matrix metalloproteinase-1 (MMP-1) inhibitory assay.

Materials and methods: The C. asiatica plant was extracted with methanol and fractionated with ethyl acetate, n-butanol and water. The enzymatic activities were evaluated using ursolic acid and oleanolic acid as standards. Isolate molecule asiaticoside was quantified in the crude extract and fractions through high-performance liquid chromatography (HPLC) and structural was characterized by liquid chromatography–mass spectroscopy (LC–MS) and ¹H nuclear magnetic resonance (NMR). Isolated compound was also evaluated for in vitro enzyme assays.

Results: Extract exhibited anti-hyaluronidase and anti-elastase activity with IC₅₀ of 19.27?±?0.37 and 14.54?±?0.39?µg/mL, respectively, as compared to ursolic acid. Centella asiatica n-butanol fraction (CAnB) and isolated compound showed significant hyaluronidase (IC₅₀?=?27.00?±?0.43 and 18.63?±?0.33?µg/mL) and elastase (IC₅₀?=?29.15?±?0.31 and 19.45?±?0.25?µg/mL) inhibitory activities, respectively, and also showed significant MMP-1 inhibition (p?<?0.05 and p?<?0.01).

Discussion and conclusion: n-Butanol fraction was found to be most effective among the all fractions from which asiaticoside was isolated and further quantified by HPLC. This work concludes that the asiaticoside from C. asiatica may be a prospective agent for skin care.     

复方积雪甙乳胶剂的研制

制备了积雪甙和甲硝唑的复方乳胶剂，该制剂对宫颈糜烂和阴道炎具有良好的顺应性和疗效，具有含油率低，冷热稳定性好的特点。     

积雪草苷的抗菌作用及对小鼠实验性泌尿系统感染的治疗作用

目的:研究积雪草苷的体内外抗苗作用。方法:采用平皿二倍稀释法测定积雪草苷对37株标准菌株及临床分离致病菌株的最低抑菌浓度(MIC);采用小鼠膀胱上行性肾感染模型,观察积雪草苷在0．68～3．36 g·kg-1剂量时灌胃给药对大肠埃希菌26的清除作用,用Bliss程序计算药物清除细菌的半数有效剂量(ED50)和95％置信限。结果:积雪草苷对37株标准及临床分离菌株显示较强的抗菌活性,尤其对各种耐药细菌,包括耐甲氧西林的金葡球菌(MRSA)、表葡菌(MRSE),耐5种氨基糖苷类抗生素、产钝化酶的粪肠球菌,产β-内酰胺酶、产超广谱β-内酰胺酶的大肠埃希菌、肺炎克雷伯杆菌和醋酸钙不动杆菌,以及耐哌拉西林的铜绿假单胞菌的MIC值与三金片相近。积雪草苷对小鼠膀胱上行性肾感染大肠埃希菌26的清除细菌作用较强,积雪草苷和三金片的ED50分别为1．87和3．16 g·kg-1(P<0．01)。结论:积雪草苷具有良好的体内、外抗菌活性,尤其对于泌尿系统感染。     

积雪草苷对脂多糖诱导小鼠急性肺损伤的影响

目的观察积雪草苷对脂多糖(LPS)诱导急性肺损伤(ALI)小鼠的保护效应。方法56只Balb/c小鼠随机分为对照、假手术、模型、溶媒、积雪草苷(5、15、45 mg/kg)7组,每组8只。LPS(2.5 mg/kg)气管滴注造ALI模型,24 h后HE染色观察小鼠肺组织病理改变,计算肺湿质量/干质量值;收集支气管肺泡灌洗液(BALF)后行白细胞计数和分类,BCA法检测其蛋白的量;化学法检测肺组织髓过氧化物酶(MPO)活性。结果积雪草苷各剂量组呈剂量依赖性减轻LPS所致ALI模型肺组织病变情况,减轻肺水肿,减轻中性粒细胞的浸润,抑制蛋白渗出。抑制肺组织中MPO活性。结论积雪草苷对LPS诱导ALI有保护效应。     

积雪苷霜软膏对兔耳增生性瘢痕组织中S mad4蛋白表达的影响

目的：探讨积雪苷霜软膏对兔耳增生性瘢痕组织中Smad4蛋白表达的影响。方法新西兰大耳白兔18只,随机分为正常对照组、模型组、积雪苷霜软膏组。模型组和积雪苷霜软膏组建立兔耳腹侧面增生性瘢痕模型,术后21 d,积雪苷霜软膏组瘢痕局部涂抹积雪苷霜软膏,每天2次,连续用药28 d;正常组和模型组涂抹软膏基质。每周观察并拍照记录瘢痕的生长情况,于术后49 d处死动物,进行病理、羟脯氨酸和Smad4蛋白检测。结果模型组瘢痕以较快速度生长,积雪苷霜软膏组,瘢痕生长显著被抑制。病理检测显示,模型组增生性瘢痕组织纤维化明显,而积雪苷治疗可显著改善纤维化。羟脯氨酸定量显示,增生性瘢痕组织中羟脯氨酸（Hyp）含量显著升高（P＜0．01）,而积雪苷治疗显著降低瘢痕组织中Hyp水平（P＜0．01）。免疫印迹结果显示增生性瘢痕组织中Smad4蛋白水平显著上调,而积雪苷治疗显著降低Smad4蛋白表达（P＜0．01）。结论积雪苷霜抗增生性瘢痕作用可能与调控Smad4蛋白表达有关。