三白草体外抗氧化活性

目的:研究三白草体外抗氧化活性.方法:采用清除二苯代苦味酰基(DPPH)自由基和[2,2′-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐](ABTS)自由基测定法,对三白草提取物抗氧化活性进行评价.结果:正丁醇提取物清除DPPH自由基(IC50=16.94)的能力比BHT清除能力(IC50=18.71 mg·L-1)强,弱于阳性对照PG和BHA清除DPPH自由基的能力(IC50分别为0.89,3.2mg· L-1);清除ABTS自由基的能力(IC50=12.90 mg·L-1)弱于阳性对照PG和BHA清除ABTS自由基的能力(IC50分别为0.81,1.95 mg·L-1),比阳性对照BHT( IC50为7.72mg· L-1)清除ABTS自由基的能力略弱;石油醚提取物和乙酸乙酯提取物清除DPPH和ABTS自由基的能力均比阳性对照PG,BHT,BHA清除DPPH和ABTS自由基的能力弱.结论:三白草正丁醇提取物有抗氧化活性.     

板蓝根不同提取部位的体外抗氧化活性

目的:考察板蓝根不同提取部位的体外抗氧化活性.方法:用大孔树脂和阴、阳离子交换树脂制备板蓝根3个有效部位:总木脂素、总有机酸、总生物碱部位.结合酶标仪,通过清除1,1-二苯基苦基苯肼(DPPH)自由基和Fe3+还原能力(FRAP)测定板蓝根提取物的体外抗氧化作用.结果:板蓝根颗粒组及各个提取物组均有不同程度的体外抗氧化效果.其中以总木脂素抗氧化效果最佳(DPPH IC50 160.6 mg·L-1;FRAP 0.75 mmol·g-1);其次为板蓝根颗粒组(DPPH IC50 595.97 mg·L-1;FRAP 0.12 mmol·g-1);总有机酸,总生物碱部位效果较弱.结论:测定了板蓝根中不同有效部位的抗氧化活性,初步确定其抗氧化相关活性成分主要存在于木脂素部位.     

灯盏细辛的抗氧化活性研究

目的:研究灯盏细辛6个化合物的抗氧化活性.方法:以灯盏细辛为原料,采用有机溶剂提取法和色谱柱法对灯盏细辛中化学成分进行提取与分离,利用核磁共振等波谱学方法进行结构鉴定,体外法检测6个化合物对1-二苯基-2-苦味酰肼自由基清除法( DPPH),2,2′-联氨-双-(3-乙基苯并噻唑啉-6-磺酸)自由基清除法(ABTS),O2·自由基和·OH自由基的清除能力,并以维生素C(Vc)为阳性对照,结果:6个化合物者对DPPH,ABTS,O-2·,·OH自由基均具有清除作用,且与浓度呈良好的量效关系,6个化合物清除自由基能力与Vc相似.结论:灯盏细辛中的6个化合物均具有较强的抗氧化活性.     

加减补肺汤联合甲强龙对IPF大鼠抗氧化作用的影响

目的:观察加减补肺汤联合甲强龙对博来霉素诱导的特发性肺纤维化(IPF)大鼠抗氧化作用,探讨其作用机制。方法:60只SD大鼠随机分为正常组,模型组,甲强龙组(10 mg·kg~(-1)·d~(-1)),加减补肺汤组(100 mg·kg~(-1)·d~(-1)),联合用药1组,联合用药2组,每组10只。除正常组外其余各组大鼠采用气管内一次性滴注博来霉素复制IPF模型(1 mg/只),造模24 h后,开始ig或im给药,持续到处死动物的前1 d,模型组ig等体积的生理盐水。计算IPF大鼠肺系数,观察肺组织形态学改变,测定各组大鼠肺组织匀浆中羟脯氨酸(Hyp),丙二醛(MDA)含量和超氧化物歧化酶(SOD)的活性,取固定部位肺组织分别进行苏木素-伊红(HE)染色。结果:与正常组比较,模型组大鼠肺泡炎细胞浸润及肺间质纤维化程度明显,肺系数明显升高,肺组织中Hyp,MDA含量明显升高、肺组织中SOD的活性明显降低(P0.05,P0.01);与模型组比较,各给药组均可改善IPF大鼠的生存状态,甲强龙组、加减补肺汤组和联合用药1组可明显改善IPF大鼠肺泡炎细胞浸润及肺间质纤维化程度,显著降低肺系数(P0.01,P0.05),降低肺组织中Hyp,MDA含量、升高肺组织中SOD的活性。结论:联合用药1组对降低IPF大鼠肺组织中肺系数,Hyp和MDA的含量,升高肺组织中SOD活性,优于单独给药,其机制可能是减少脂质过氧化物的释放而达到抗氧化作用。     

氧化应激致心室重构的研究进展

心室重构意味着血管壁中氧化应激水平的提高,当机体受到有害刺激时,氧化和抗氧化之间失衡,导致大量活性氧簇（ ROS）的产生,加速心室重构的进展。近年来,新型抗氧化剂成为治疗或预防心室重构的靶点,观察抗氧化剂治疗在心室重构患者的长期应用是当下趋势。该文就氧化应激与心室重构之间的关系及相关药物治疗进行综述。     

Nutritional and nutraceutical characteristics of Sageretia theezans fruit

The fruit of Sageretia theezans is one of many underutilized edible fruits that grow along the southern seashores of East Asia. In this study, to evaluate the nutritional and nutraceutical values of S. theezans fruit, the composition of minerals, organic acids, and proximate and fatty acids, the total phenolic, total flavonoid, and total anthocyanin content, and the antioxidant and antidiabetic activities of S. theezans fruit were analyzed. The results indicate that S. theezans fruit could be classified as a potential potassium-, malic acid-, and linoleic/oleic acid-rich fruit. In addition, The ethyl acetate (EtOAc) fraction of the 70% ethanol (EtOH) crude extract exhibited strong antioxidant activities including free radical scavenging and reducing power activities compared with the same concentration of butylated hydroxytoluene. Furthermore, the EtOAc fraction showed significant inhibition of α-glucosidase activity. The analysis of the total phenolic and flavonoid content suggested that the remarkable antioxidant and antidiabetic activities of the EtOAc fraction are due to the presence of high levels of polyphenolic compounds.     

By-product of Lavandula latifolia essential oil distillation as source of antioxidants

The objective of this work was to evaluate the antioxidant properties of Lavandula latifolia waste obtained after essential oil distillation. Samples of 12 wild populations of the Lavandula genus collected between 2009 and 2010 were hydrodistilled and their by-products were analyzed using the Folin–Ciocalteu, free radical scavenging activity (2,2-diphenyl-1-picrylhydrazyl), and the ferric reducing antioxidant power (FRAP) methods. Rosmarinic acid, apigenin, and luteolin contents were analyzed by high-performance liquid chromatography–diode array detection. The mean of total phenolic content ranged from 1.89 ± 0.09 mg gallic acid equivalents/g dry weight to 3.54 ± 0.22 mg gallic acid equivalents/g dry weight. The average value of the half maximal effective concentration (EC₅₀) for scavenging activity ranged from 5.09 ± 0.17 mg/mL to 14.30 ± 1.90 mg/mL and the variability of the EC₅₀ in FRAP ranged from 3.72 ± 0.12 mg/mL to 18.55 ± 0.77 mg/mL. Annual variation was found among this samples and the environmental conditions of 2009 were found to be more favorable. The plants collected from Sedano showed the highest antioxidant power. Our results show that rosmarinic acid and apigenin in L. latifolia contributed to the antioxidant properties of the waste. In conclusion, the by-product of the distillation industry could be valorizing as a source of natural antioxidants.     

Therapeutic effects of puerarin on polycystic ovary syndrome: A randomized trial in Chinese women

Background:This study aims to assess the therapeutic effects of a well-known component (puerarin) obtained from a Chinese herb root in patients with polycystic ovary syndrome (PCOS).Methods:Women with premature ovarian failure (POF) were assigned to the obese group (body mass index [BMI] ≥24 kg/m² and waist hip ratio [WHR] >0.85) or non-obese group (group 3, n = 21). Obese patients were further randomly assigned to the obese treatment group (group 1, n = 15) and obese control group (group 1, n = 15). All patients received standard treatment (Diane-35, 1 tablet/d, orally, plus metformin, 1.5 g/d, orally). In addition to the standard modality, patients in group 1 and group 3 also orally received 150 mg/d of puerarin tablets for 3 months. Venous blood was drawn before and after treatment. Then, the metabolic and antioxidant biomarkers were measured. The normality of distribution of the data was tested using the Kolmogorov–Smirnov method. The baseline characteristics were analyzed using one-factor analysis of variance (ANOVA), and post-hoc was performed using the least significance difference (LSD)-t test.Results:Significantly improved blood levels of sex hormone binding globulin (SHBG) and superoxide dismutase (SOD) were observed in patients who received the additional treatment of puerarin, regardless of their lean or obese status, while these were not observed in patients who did not receive puerarin. Furthermore, obese patients with PCOS had significantly lower systolic blood pressure, total cholesterol, and testosterone blood levels, when compared with before treatment.Conclusion:The addition of puerarin to the present treatment protocol can be considered for the management of metabolic disorders and hyperandrogenism in PCOS patients.