经动脉化疗栓塞兔VX2肝癌后早期肿瘤细胞凋亡

目的　研究介入治疗兔VX2肝癌肿瘤细胞凋亡的早期动态改变 .方法　建立 2 7只新西兰大白兔VX2肝癌模型 ,随机分成肝动脉化疗栓塞组 (Transarterialchemoembolization ,TACE)、灌注化疗组 (Transarterialinfusion ,TAI)、灌注肝素生理盐水组 (对照组 ,Control) ,每组各 9只 .介入治疗后每组再随机分成 3个亚组 ,每亚组 3只 .在治疗后第2 4、72、12 0小时分别处死 3组中一亚组 ,取材肿瘤生长活跃的外带组织用流式细胞仪AnnexinV和PI双标记法定量检测细胞凋亡 ,组织病理切片苏木精 -伊红染色 (HE染色 )和甲基绿 -派洛宁染色 (MG -P染色 )光镜下形态学方法观察不同时间肿瘤细胞的凋亡变化 .结果　TACE、TAI、Control组在治疗后 2 4、72、12 0小时流式细胞仪法定量检测肿瘤细胞凋亡比率分别为 11.4 4± 2 .15、10 .99± 1.74、6 .0 0± 0 .5 8,5 .84± 0 .6 8、4 .6 5± 0 .11、2 .88± 1.2 3和 2 .80± 0 .15、2 .19± 1.6 9、2 .5 1± 2 .13.TACE、TAI、Control组凋亡在各时间点均有显著差异 (p <0 .0 1) ,TACE组 2 4、72小时的凋亡明显高于 12 0小时 (p <0 .0 5 ) .TACE组诱导的肿瘤细胞凋亡比率明显高于TAI、Control组 ;TACE组诱导的肿瘤细胞早期凋亡比率随时间呈下降趋势 ,在 12 0小时凋亡仍明显高于其     

炎症性肠病动物模型的研究概况

The etiology and pathogenesis of inflammatory bowel disease are up to now still not clear and definite. Establishing the ideal animal model to study its cause and pathogenesis of this disease is very important. The ideal animal model should have the same manifestation with human inflammatory bowel disease on clinical and pathologic feature etc. In this article, the method, the pathologic character isfics and concerning pathogenesis, of a few common useful experiment animal models are discussed.     

Macrophage and perisinusoidal cell kinetics in acute liver injury.

Perisinusoidal cells (PSCs) are currently regarded as the major source of extracellular matrix proteins during hepatic fibrogenesis in response to liver injury. However, the cellular mechanisms underlying their response to injury are not fully understood. One hypothesis is that the PSCs are stimulated by peptide growth factors produced by hepatic macrophages (Kupffer cells) in response to parenchymal cell damage. In this study we have investigated the kinetics of the PSC and macrophage populations in acute carbon tetrachloride-induced hepatic injury in rats. PSCs were identified immunohistochemically by detection of cytoplasmic desmin; monocytes and macrophages were detected using the monoclonal antibodies ED1 and ED2; cells in S phase were identified by immunohistochemical detection of nuclear-incorporated bromodeoxyuridine. The results showed an expansion of the desmin-positive PSC population, predominantly within the damaged perivenular zones, which reached a peak on days 3 and 4 following administration of carbon tetrachloride; this was contributed to by local PSC proliferation. The PSC response was preceded by an expansion of the macrophage population resulting from both local macrophage proliferation and influx of blood monocytes. These results are in keeping with the hypothesis that the PSC response to acute liver injury is mediated, at least in part, by hepatic macrophages.     

Murine acinar pancreatitis preceding necrotizing myocarditis after Coxsackievirus B3 inoculation.

Balb/C weanling mice were inoculated intraperitoneally with a myocarditic variant of coxsackie-virus B3, with the aim of characterizing more fully the cell damage induced in the heart as well as in other organs. During the first week postinfection (pi), all animals developed acinar pancreatitis, followed by focal myocarditis. In accordance with the increasing infectivity titers, such progressive histopathological changes correlated with local viral replication. From day 4 pi, acinar degeneration accompanied by diffuse inflammatory exudate was observed in the pancreas, followed by fatty tissue replacement by day 8. In the heart, focal necrosis rather than inflammatory reaction first appeared at 4 days pi and became widespread by 6-8 days pi. Necrotic foci usually presented calcium deposits, with absence of myofibrils in the affected fibers. The fact that both periodic acid Schiff (PAS) and Best carmine staining remained positive even after diastase treatment ruled out basophilic necrosis. In summary, the pancreas appeared to be the site of primary viral replication leading to viremia.     

药物洗脱支架用于急性心肌梗死的临床观察

目的评价药物涂层支架用于急性心肌梗死的安全性及临床疗效。方法34例发病1周以内的急性心肌梗死患者梗塞相关动脉机械性再灌注治疗时使用药物涂层支架,并于术后3～12个月进行门诊及电话随访,必要时复查冠状动脉造影,观察住院及随访期间心血管事件发生率(死亡、再发急性心肌梗死及血管重建术)。结果37枚药物涂层支架(CYPHER15枚,TAXUS22枚)植入34个梗塞相关动脉(其中3处病变各植入2枚支架),支架长度(22.7±9.0)mm,支架直径(3.1±0.3)mm;10例(29.4%)接受直接经皮冠状动脉介入治疗(PCI),3例(8.8%)为挽救性PCI。支架植入成功率100%。1例(2.9%)支架内急性血栓形成,行急诊血管重建术。平均随访间期(5.9±2.8)个月,2例(5.9%)于随访期间因再发原部位心肌梗死入院治疗,1例因非靶血管病变致心绞痛于术后10个月入院治疗。住院及随访期间无死亡病例发生。结论研究提示药物涂层支架用于急性心肌梗死患者治疗梗塞相关动脉行机械性再灌注安全,可获得与药物涂层支架用于择期的、相对简单的冠状动脉病变的类似疗效。     

心肌梗死患者窦性心律震荡的检测及其临床意义

目的：探讨心肌梗死（心梗）患者窦性心律震荡检测的临床意义。方法：测定24小时动态心电监测的170例心梗患者和116例正常人室性早搏（室早）后的震荡初始（TO）和震荡斜率（TS）值及平均值，并进行对比分析。结果：心梗患者室早后的TO值明显高于对照组、鸭值明显低于对照组（P〈0．05和P〈0．01），TO和／或TS异常组死亡率明显高于TO和鸭均正常组（P〈0．001）。结论：心梗后患者窦性心律震荡现象减弱或消失时，其猝死的发生率增高，及时检测心梗患者室早后的TO和TS值，对发生猝死的高危患者的检出、危险度分层以及对其进行干预治疗都有重要的临床意义。     

基于膝关节三维模型的髌股关节动力学特性研究

目的构建有效的膝关节三维模型以计算髌股关节动力学参数。方法基于CT图像处理和CAD技术, 重建包括股骨下端、胫骨上端及髌骨的膝关节的骨骼三维模型,并定义髌股关节运动起重要作用的股四头肌直线模型、韧带及其它软组织的非线性弹性纤维束模型,以股四头肌肉力为输入控制变量。结果构造出一个有效的膝关节三维模型。结论该模型可以有效的计算髌股关节动力学参数。     

蝎蜂毒肽对大鼠纤溶系统作用初探

本研究采用大鼠肢体血管灌流和整体给药两种模型，观察蝎蜂毒（ＳＢＰ）对血管理灌流液内纤溶酶原激活物（ＰＡ）活性、血浆优球蛋白纤溶性（ＥＦＡ）和纤溶酶（ＰＬ）活性的影响。结果说明，ＳＢＰ有明显激活纤溶系统作用；其机制可能涉及血管内皮细胞释放ＰＡ活性增加，进一步促使纤溶酶原活化为ＰＬ增多的途径。     

Effect of pregnancy on proteoglycan-induced progressive polyarthritis in BALB/c mice: remission of disease activity

Proteoglycan-induced arthritis is a murine autoimmune model displaying many similarities to human rheumatoid arthritis and ankylosing spondylitis, as has been documented by clinical, immunological and histopathological studies. Since the onset of arthritis correlates with the serum antibody level to mouse cartilage proteoglycan (PG), it is believed that these autoreactivc antibodies may play crucial roles in the pathological mechanisms of PG-induced arthritis. We have found that fertility in these PG-induced arthritic mice had been reduced but, unlike collagen-induced arthritis, had not been completely lost. Moreover, pregnancy had a beneficial effect upon the clinical symptoms with very little or no influence on scrum antibody levels. Although fertility was retained and arthritic mothers delivered healthy offspring, the birth frequency was significantly less than in non-arthritic age-matched controls. Furthermore, the presence of anti-PG autoantibodies (predominantly IgG1 subclass) transmitted from arthritic mothers to infants transplacentally and by milk during the lactation period did not render these offspring either resistant or more sensitive to subsequent induction of arthritis. Subsequent immunization of infants with ‘arthritogenic’ PG revealed an unaltered susceptibility to arthritis induction.     

Use of monoclonal antibodies against myosin light chains 1 to detect the corresponding antigen in blood of patients with myocardial infarction

Research Institute of Human Genetics, All-Union Medical Genetics Research Center, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR A. D. Ado.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 112, No. 10, pp. 416–417, October, 1991.