益血晶颗粒含量测定方法的研究

目的 建立益血晶颗粒的质量控制方法。方法 环黄芪烷醇类皂苷用氢氧化钾脱乙酰基而使所有皂甙全部变成黄芪甲甙的前处理方法，以氯仿－甲醇－水（15：7：2）为展开剂，双波长薄层扫描法测定黄芪甲甙含量，测定波长为500nm，参比波长为700nm；血红素铁用比色法测定，测定波长：386nm。结果 环黄芪烷醇类皂苷和血红素铁平均回收率97.87%(RSD=2.34%)和100.77%(RSD=2.41%)。结论 方法简单、准确，可用于该制剂的质量控制。     

Role of GABAB receptors in intracellular Ca2+ homeostasis and possible interaction between GABAA and GABAB receptors in regulation of transmitter release in cerebellar granule neurons

The expression of GABA_B receptors in cultured mouse cerebellar granule cells was investigated in binding experiments using [³H](S, R)-baclofen as well as in functional assessment of the ability of (R)-baclofen to interact with depolarization (15–40 mM KCI) coupled changes in intracellular Ca²⁺ homeostasis and neurotransmitter release. In the latter case a possible functional coupling between GABA_A and GABA_B receptors was investigated. The binding studies showed that the granule cells express specific binding sites for (R)-baclofen. The number of binding sites could be increased by exposure of the cells to the GABA_A receptor agonist THIP (4,5,6,7-tetrahy-droisoxazolo[5,4-c]pyridin-3-ol) during the culture period. Pretreatment of the neurons with pertussis toxin showed that the GABA_B receptors are coupled to G-proteins. This coupling was, however, less pronounced when the cells had been cultured in the presence of THIP. When ⁴⁵Ca²⁺ uptake was measured or the intracellular Ca²⁺ concentration ([Ca²⁺]_i) determined using the fluorescent Ca²⁺ chelator Fluo-3 it could be demonstrated that culturing the neurons in THIP influences intracellular Ca²⁺ homeostasis. Moreover, this homeostasis was found to be functionally coupled to the GABA_B receptors as (R)-baclofen inhibited depolarization-induced increases in ⁴⁵Ca²⁺ uptake and [Ca²⁺]_i. (R)-Baclofen also inhibited K⁺-induced transmitter release from the neurons as monitored by the use of [³H]D -aspartate which labels the neurotransmitter pool of glutamate. Using the selective GABA_A receptor agonist isoguvacine it could be demonstrated that the GABA_B receptors are functionally coupled to GABA_A receptors in the neurons leading to a disinhibitory action of GABA_B receptor agonists. © 1994 Wiley-Liss, Inc.     

Cutaneous Disseminated Actinomycosis in a Patient with Acute Lymphocytic Leukemia

Actinomycosis is an uncommon infectious disease caused predominantly by Actinomyces israelii. The cutaneous disseminated form is usually caused by hematogenous dissemination from a primary extra-cutaneous lesion. We report here cutaneous disseminated actinomycosis without any detectable extra-cutaneous lesions in a 42-year-old Japanese woman with acute lymphocytic leukemia. Multiple soft nodules developed on her upper and lower extremities. Histopathological examination revealed “sulfur granules”, which are a specific finding for actinomycosis. Cultures from biopsy specimens were not successful. There were no cervicofacial, thoracic, nor abdominal lesions. These findings suggest that cutaneous disseminated actinomycosis in our patient developed primarily in the skin. Although the patient was immunocompromised, antibiotic treatment with minocycline was effective.     

Ionic and osmotic dependence of secretion from permeabilised acini of the rat pancreas

Many types of secretory granule have been observed to swelll as a result of cell stimulation implying a degree of osmotic control, although the regulation of granule fusion with the apical plasma membrane is not clearly understood. In the present study we have investigated the ionic and osmotic dependency of basal and stimulated³H-protein release from rat pancreatic acini, permeabilised by either digitonin or high voltage electric discharge. Acini were stimulated with either cholecystokinin-pancreozymin octapeptide (CCK-8), carbachol (CCh), or with phorbol ester (TPA) plus cAMP. Stimulated secretion was significantly reduced when 130 mmol/l Cl^– in the buffer was replaced by I^–, NO ₃ ^– , SCN^– or cyclamate^–. Secretion in Cl^– buffers was inhibited by the anion transport inhibitor 4,4-diisothiocyanatostibene-2,2-disulfonic acid (DIDS), by 40% of the control response. Neither Na⁺ nor N-methyl-d-glucamine^– could replace K⁺ in the buffer. Ba²⁺ and quinine, which block K⁺ conductance pathways, inhibited stimulated secretion by 50%. Finally, stimulated secretion from leaky cells was nearly abolished by doubling buffer osmolarity. The data suggest that when the cell is stimulated, a Cl^– and a K⁺ permeability appear in the zymogen granule membrane and the ions enter down their electrochemical gradients. The increased intragranular osmolarity results in granular swelling which is intimately associated with secretion.Parts of this study have been previously presented at the XIXth Meeting of the European Pancreatic Club (1987) and at the 65th Meeting of the Deutsche Physiologische Gesellschaft (1988)     

中药扶正联合化疗法治疗晚期非小细胞肺癌的疗效观察

目的评价中药扶正联合化疗法治疗晚期非小细胞肺癌的治疗价值。方法68例晚期非小细胞肺癌患者随机分为治疗组38例和对照组30例。对照组单用MVP方案，治疗组在此基础上加用中药扶正，疗程4周至8周。结果治疗组总有效率为52．6％，对照组30％，两组比较差异有显着性意义（P〈0．05）。结论提示中药扶正联合化疗治疗晚期非小细胞肺癌有较好疗效。     

通栓扶正胶囊治疗脑梗死的临床与实验研究

目的 :研究通栓扶正胶囊对脑梗死患者临床疗效与溶栓、免疫功能的影响。方法 :将 146例脑梗死患者随机分为观察组和对照组。观察组常规治疗加口服通栓扶正胶囊 ,30 d后对两组治疗前、后神经功能缺损程度、疗效及有关实验室检查指标进行比较 ,对其数据进行统计学处理。结果 :两组治疗后 ,神经功能缺损程度相比有显著差异 (P<0 .0 5 ) ;治疗组总有效率 95 .6 8% ,对照组总有效率 6 3.33% ,经 U检验 (P<0 .0 1) ,差异显著 ;两组治疗后各实验室检查指标对比 CD3+ 、CD4+ 、CD8+ 、CD4+ / CD8+ 、PAGm、血浆粘度、红细胞聚集指数差异显著。结论 :通栓扶正胶囊即可溶解血栓 ,又可调节机体免疫功能。     

双扑伪麻泡腾颗粒剂的药效学实验研究

目的：对双扑伪麻泡腾颗粒剂的药效学进行研究。方法：通过家兔致热试验、小白鼠醋酸扭体法、小白鼠热板法、小鼠耳壳二甲苯致肿试验、毛细血管通透性试验及豚鼠鼻粘膜致敏试验等方法对双扑伪麻泡腾颗粒剂的药效学进行评价。结论：双扑伪麻泡腾颗粒剂是一抗感冒疗效高、副作用小的新剂型。     

Clinical Observation of Tiaojining granule combined with corticosterone in treating infantile primary nephrotic syndrome) granule combined with corticosterone in treating infantile primary nephrotic syndrome

Objective: To investigate the effects of Tiaojining granule TJNG) combined with corticosterone (CS) in treating infantile primary nephrotic syndrome (IPNS).Methods: Sixty inpatients with IPNS were divided into two groups, 30 cases as the treated group treated by TJNG combined with CS, and the other 30 cases as the control group treated by CS alone for 8 weeks. The changes of urinary protein, serum albumin, blood cholesterol, platelet and blood pressure before and after treatment were observed.Results: The total effective rate of the treated group was significantly higher than that of the control group (P<0.01). The time for urinary protein disappearance of the treated group was significantly shorter than that of the control group (P <0.05). In preventing hypertension, lowering lipidemia and platelets, TJNG were obviously better than CS used in the control group (P<0.01).Conclusion: TJNG could enhance the therapeutic effects of CS on IPNS and safely and effectively reduce the side-effects of CS.     

HPLC法测定解酒灵冲剂中葛根素的含量

应用ＨＰＬＣ法测定解酒灵冲剂中葛根素的含量，该方法简便、准确、重现性好，可作为该制剂控制质量标准的含量测定方法