脑络欣通和左归丸药物血清对体外培养的大鼠神经干细胞增殖分化的作用

目的 探讨脑络欣通药物血清和左归丸药物血清对体外培养的大鼠胚胎神经干细胞(neural stem cell, NSC)增殖分化的作用。方法 分别采用含10%脑络欣通药物血清和含10%左归丸药物血清的培养基培养大鼠胚胎NSC,观察其促增殖效应和诱导分化效应,应用相差显微镜和免疫荧光染色对其进行比较观察。结果 脑络欣通药物血清和左归丸药物血清均可诱导绝大多数NSC分化成神经元、星形胶质细胞和少突胶质细胞。此外,两者还能在一定程度上促进培养的NSC生长。前者诱导NSC分化的进程虽比后者要慢,但诱导NSC向神经元方向分化的比率较高,其分化的神经元在细胞形态学上与发育成熟的神经元更为接近;后者促进NSC生长的效应较为显著。结论 脑络欣通和左归丸均能促进体外培养的NSC生长和分化,两者作用有一定差异;益气活血治法和补肾生髓治法对NSC进行诱导分化具有一定的可行性。     

芪参益气滴丸干预糖尿病大鼠虹膜微血管病变的作用机制研究

目的 采用激光共聚焦显微镜超速摄像技术和荧光白蛋白标记技术,动态观察芪参益气滴丸干预糖尿病大鼠虹膜微血管病变的作用机制。方法 链脲佐菌素（STZ）腹腔注射建立1型糖尿病大鼠模型,随机分为模型组、治疗组、对照组,并设正常组。治疗组灌服芪参益气药液,对照组灌服多贝斯液［1 g/ (kg·d）］,疗程10个月。利用激光共聚焦显微镜超速摄像动态观察虹膜微循环状态。结果 治疗组虹膜血流速度明显加快,虹膜血管内、外荧光密度降低,荧光渗漏面积及虹膜血管管径明显缩小,与模型组比较,差异均有统计学意义（P<0.01）。结论 芪参益气滴丸在改善虹膜微血管流态方面的确有一定作用,可以促进血流加快,抑制血管异常扩张及改善虹膜微血管的通透性异常增高状态。     

Wettability of denture relining materials under water storage over time

STATEMENT OF PROBLEM

Poor wettability of denture relining materials may lead to retention problems and patient discomfort.

PURPOSE

Purpose of this study is to compare and evaluate wettability of nine denture relining materials using contact angle measurements under air and water storage over time.

MATERIAL AND METHODS

Nine denture relining materials were investigated in this study. Two heat-curing polymethyl-methacrylate (PMMA) denture base materials: Vertex RS, Lang, one self-curing polyethyl-methacrylate (PEMA) chairside reline resin: Rebase II, six silicone relining materials: Mucopren soft, Mucosoft, Mollosil® plus, Sofreliner Touch, GC Reline™ Ultrasoft, Silagum automix comfort were used in this experiment. Contact angles were measured using high-resolution drop shape analysis system (DSA 10-MK2, KRUESS, Germany) under three conditions (in air after setting, 1 hour water storage, and 24 hours water storage). Nine materials were classified into three groups according to material composition (Group 1: PMMA, Group 2: PEMA, Group 3: Silicone). Mean values of contact angles were compared using independent samples t-test and one-way ANOVA, followed by a Scheffe''s post hoc analysis (α= 0.01).

RESULTS

Contact angles of materials tested after air and water storage increased in the following order: Group 1 (PMMA), Group 2 (PEMA), Group 3 (Silicone). Heat-cured acrylic denture base resins had more wettability than silicone relining materials. Lang had the highest wettability after 24 hours of water storage. Silicone relining materials had lower wettability due to their hydrophobicity. Wettability of all denture relining materials, except Rebase II and Mollosil® plus, increased after 24 hours of water storage.

CONCLUSIONS

Conventional heat-cured resin showed the highest wettability, therefore, it can be suggested that heat-cured acrylic resin is material of choice for denture relining materials.     

Effect of corpus callosotomy on attention deficit and behavioral problems in pediatric patients with intractable epilepsy

To evaluate the effect of corpus callosotomy (CC) on attention deficit and behavioral problems in pediatric patients with intractable epilepsy, we retrospectively investigated sequential patients who had undergone CC to control seizures. Between August 2005 and April 2010, a total of 15 patients aged between 3.1 and 17.9 years underwent CC at our institute. All the patients experienced either drop attacks or head nodding, which were considered to be therapeutic targets of CC. A standardized instrument, the Child Behavior Checklist (CBCL), was used to assess behavioral and emotional problems before and after surgery. On postoperative EEGs, 8 (53%) showed improvement and 7 (47%) showed no change in epileptiform discharges. The Attention Problems scale and total score on the CBCL significantly improved in patients whose postoperative EEGs showed improvement. In addition to amelioration of target seizures, CC can improve attention impairments in association with improvement in the postoperative EEG.     

复方丹参滴丸治疗老年性冠心病心绞痛78例临床分析

目的：观察复方丹参滴丸对老年性冠心病心绞痛的疗效。方法：将78例患者随机分为两组,均常规服用单硝酸异山梨酯片、阿司匹林肠溶片等,心绞痛发作时可临时含服硝酸甘油;治疗组加服复方丹参滴丸10粒,每日3次,两组均以4周为1个疗程。结果：两组在缓解心绞痛症状、心电图改善方面与对照组比较,差异均有统计学意义（P〈0.05）。治疗后6个月内临床心脏事件发生率治疗组低于对照组。结论：复方丹参滴丸治疗老年性冠心病心绞痛疗效确切,可降低心绞痛的发生率和心脏事件的发生率。     

心理护理联合复方丹参滴丸治疗冠心病抑郁患者临床疗效观察

目的:探讨心理护理联合复方丹参滴丸治疗冠心病抑郁患者临床治疗效果及对负性心理情绪的影响。方法:选择我院治疗的冠心病抑郁患者80例,随机分为观察组和对照组,对照组给予西医常规治疗联合心内科常规护理,观察组在对照组治疗和护理的基础上口服复方丹参滴丸并给予心理护理,观察两组患者临床疗效。结果:观察组患者治疗的效果优于对照组,经统计学分析比较,差异有统计学意义(P<0.05)。观察组患者ST段压低持续时间和24 h压低次数均少于对照组,经统计学分析比较,差异有统计学意义(P<0.05)。观察组患者临床护理满意度高于对照组,经统计学分析比较,差异有统计学意义(P<0.05)。结论:采用心理护理联合复方丹参滴丸治疗冠心病抑郁患者疗效可靠,降低患者ST段压低持续时间和24h压低次数,提高临床护理满意度,值得在临床大力推广使用。     

通络宁心口服液治疗不稳定性心绞痛的临床观察

目的对通络宁心口服液治疗不稳定性心绞痛(UA)的疗效及安全性作出评价。方法将55例冠心病心绞痛患者随机分为2组:治疗组为31例,用通络宁心口服液,每次20 mL,8 h 1次;对照组24例,给予复方丹参滴丸10粒/次,每日3次,疗程1个月。结果通络宁心口服液的总有效率为58.06%和对照组的58.33%相近,显效率为19.35%,优于对照组的8.89%;起效时间、症状改善、心功能改善均优于对照组。结论通络宁心口服液治疗不稳定性心绞痛疗效显著,是一种安全有效、可解除心肌缺血、缓解心绞痛的中药制剂。      

心通口服液对犬心脏血流动力学及心肌耗氧量的影响

目的：观察心通口服液对犬心脏血流动力及心肌耗氧量的影响。方法：用电磁流量计分别测量冠脉血流量及心输出量,用载波放大器测定左室内压,用血氧计测量血氧含量。结果：心通口服液可升高左室内压,增加左室作功,外周阻力下降。结论;心通口服液可明显降低心肌耗氧量及冠脉阻力,对心肌缺血有明显的保护作用。     

联用心通口服液与硝酸异山梨醇酯治疗冠状动脉粥样硬化性心脏病心绞痛疗效观察

目的：探讨联用中药心通口服液与西药硝酸异山梨醇酯治疗冠状动脉粥样硬化性心脏病（冠心病）心绞痛的疗效。方法：将６８ 例冠心病心绞痛患者随机分为２ 组。治疗组（３６ 例）用中药心通口服液与西药硝酸异山梨醇酯治疗,对照组（３２ 例）单用硝酸异山梨醇酯治疗;药物剂量：心通口服液１０ ｍ ｌ,硝酸异山梨醇酯１０ ｍ ｇ,均为每日３ 次口服,３ 周为１ 个疗程。结果：治疗组及对照组临床症状改善总有效率分别为８８９％ 及６１８％ ,有显著性差异（ Ｐ＜ ００５）;心电图改善总有效率分别为８０６％ 及５３１％ ,亦有显著性差异（ Ｐ＜ ００５）。结论：心通口服液与硝酸异山梨醇酯合用治疗冠心病心绞痛对心绞痛症状及心电图的心肌缺血改善均优于单用硝酸异山梨醇酯。