Doehlert设计法优化紫珠中苯乙醇苷的提取工艺

目的:研究乙醇回流法提取枇杷叶紫珠中苯乙醇苷的优化工艺。方法:以料液比、提取时间、乙醇体积分数为3个考察因素,苯乙醇苷含量为指标,运用响应面分析法中的Doehlert设计法进行试验设计,并采用统计学和数学软件对试验数据进行分析,拟合出苯乙醇苷类的最优提取工艺条件。结果:优选的提取条件为以12倍量90%乙醇回流提取2次,每次2 h。结论:应用Doehlert设计的响应面优化法具有使用方便、预测性好的特点,值得推广应用。     

二苯乙烯苷对大鼠骨密度与骨强度的影响

目的：检测由何首乌中提取的有效成分2,3,5,4＇-四羟基二苯乙烯-2-O-β-D葡萄糖苷（二苯乙烯苷）,长期给药对大鼠骨密度及骨强度的影响。方法：大鼠分别按剂量150 mg/kg、300 mg/kg、600 mg/kg连续灌胃给药90 d,使用双能X线骨密度扫描仪测量大鼠骨密度后,处死取股骨测量骨9骼强度。结果：二苯乙烯苷各剂量组均能增加髂骨段的骨密度,增加髂骨段和肱骨段的骨矿重量和骨矿面积;显著增加骨折力与骨碎力。结论：二苯乙烯苷不仅能够增强骨密度,提高单位面积骨矿的含量及骨矿在组织中的含有面积,增强骨组织对于外力作用的抵抗能力,提高结构韧性与结构强度,为临床有效预防并改善骨质疏松症提供实验依据。     

10批栀子中环烯醚萜苷类成分含量测定

目的 测定不同产地的10批栀子药材中栀子苷、京尼平龙胆二糖苷及总环烯醚萜苷含量。方法 采用HPLC测定栀子苷、京尼平龙胆二糖苷含量;采用紫外分光光度法测定总环烯醚萜苷含量。结果 江西产栀子中环烯醚萜苷类成分含量高。结论 不同产地栀子药材中环烯醚萜苷类成分含量有所差别。     

脑苷肌肽改善阿尔兹海默氏病小鼠病情的机理分析

目的通过检测脑苷肌肽对阿尔兹海默氏病（Alzheimer＇s disease,AD）小鼠的药理作用,观察其对于阿尔兹海默氏病病情改善情况,为建立AD临床治疗奠定理论基础。方法通过检测氧化应激水平和炎症细胞因子,结合硫磺素S染色和Morris水迷宫实验测试,观察脑苷肌肽对AD小鼠病情的影响。结果脑苷肌肽可以有效恢复部分因AD所导致的氧化应激水平及TNF-α的升高,减少大脑皮层的淀粉样斑块,提高学习记忆能力和空间位置定位能力得到提高。结论脑苷肌肽可以有效缓解AD小鼠病情,改善其神经功能,提高其记忆学习能力,可能是治疗AD的潜在药物。     

126例脑出血内科治疗疗效分析

目的：探讨采用不同的内科方法治疗脑出血的疗效。方法：126例脑出血患者采用不同的内科治疗方法进行治疗,高血压患者77例中,降压药治疗31例,非降压治疗46例;甘露醇脱水治疗87例·9脑苷肌肽与脑蛋白水解物治疗59例,其中脑苷肌肽治疗9例,脑蛋白水解物治疗50例;微创穿刺治疗18例,非微创穿刺治疗108例,对不同治疗方法的疗效进行比较。结果：126例患者治疗后有效110例（87．30％）,无效12例,死亡4例。降压药治疗有效率（87．10％）与非降压治疗有效率（93．48％）差异无统计学意义（P〉0．05）,甘露醇脱水每次用量250mL有效率（85．00％）与每次用量125mL有效率（86．57％）差异无统计学意义（P〉0．05）,脑苷肌肽治疗有效率（77．78％）与脑蛋白水解物治疗有效率（94．00％）差异无统计学意义（P〉0．05）,微创穿刺治疗有效率（88．89％）与非微创穿刺治疗有效率（87．04％）差异无统计学意义（P〉0．05）。结论：采用降压、脱水、微创穿刺及应用脑苷肌肽、脑蛋白水解物等脑保护剂类药物治疗脑出血具有一定的疗效。     

Resin glycosides from the leaves and stems of <Emphasis Type="Italic">Ipomoea digitata</Emphasis>

Alkaline hydrolysis of the ether-soluble resin glycoside (jalapin) fraction of the leaves and stems of Ipomoea digitata L. (Convolvulaceae) gave six organic acids, isobutyric, (S)-2-methylbutyric, tiglic, n-decanoic, n-dodecanoic, and cinnamic acids, and two glycosidic acids, quamoclinic acid A and operculinic acid A. Further, a new genuine resin glycoside, named digitatajalapin I, was isolated from the jalapin fraction, along with three known resin glycosides. Their structures have been determined on the basis of chemical and spectroscopic data.     

Effect of phenylethanoid glycosides and polysaccharides from the seed of Plantago asiatica L. on the maturation of murine bone marrow-derived dendritic cells

The seed of Plantago asiatica L. is one of the most popular folk herbal medicines used in China and other Asian countries. In this study, phenylethanoid glycosides and polysaccharides were isolated from the seed of P. asiatica L. by using phytochemical investigation methods. A screening model of immunological activity by using dendritic cells as target cells was established to investigate the effects of these compounds on the phenotypic and functional maturation of dendritic cells. Compared with untreated cells, dendritic cells treated with acteoside, isoacteoside or polysaccharides expressed higher level of class II MHC and costimulatory molecule CD86 (B7-2). Functional maturation was confirmed by decreased endocytosis and increased naïve T cell stimulatory activity of dendritic cells. These results showed that acteoside, isoacteoside and polysaccharides from the seed of P. asiatica L. had significant immunoenhancing activity by inducing the maturation of dendritic cells.     

New anthracene glycosides from <Emphasis Type="Italic">Rhodomyrtus tomentosa</Emphasis> stimulate osteoblastic differentiation of MC3T3-E1 cells

Two new anthracene glycosides (1, 2) were isolated from aerial parts of Rhodomyrtus tomentosa, along with three known compounds (3–5). The structures of two new compounds were established to be 4,8,9,10-tetrahydroxy-2,3,7-trimethoxyanthracene-6-O-β-D-glucopyranoside (1) and 2,4,7,8,9,10-hexahydroxy-3-methoxyanthracene-6-O-α-L-rhamnopyranoside (2) based on spectroscopic and chemical methods. Among them, compound 1, 2, and 5 significantly (P<0.05) increased the alkaline phosphatase activity, collagen synthesis, and mineralization of the nodules of MC3T3-E1 osteoblastic cells compared to those of the control, respectively.     

HPLC法测定胃灵冲剂中芍药苷和甘草苷的含量

目的 建立胃灵冲剂中芍药苷和甘草苷的含量测定方法.方法 采用HPLC法,色谱柱为Hypersil ODS2(5μm,4.6 mm ×200 mm)柱,流动相为乙腈-0.1％(体积分数)磷酸溶液(体积比14∶86),检测波长为237 nm,流速为1.0 mL·min-1.结果 芍药苷的线性范围为0.1140 ～0.570...     

花刺参中两个新的四环三萜化合物

目的从花刺参Stichopus variegatus中分离活性皂苷类成分。方法将花刺参体内得到的总皂苷溶于吡啶-2-二氧六环(1︰1)的混合液中,130℃加热2.5 h,应用多种色谱技术对回流产物进行分离纯化,得到2个三萜皂苷类化合物。结果应用现代光谱技术(2D-NMR和ESI-MS)和化学方法鉴定其结构,分别为3-O-[β-D-吡喃木糖(1→2)-4′-O-磺酸钠-β-D-吡喃木糖]-海参烷-9-烯-3β,12α,17α,25β-四醇,命名为花刺参苷A(variegatuside A,1);3-O-β-D-吡喃木糖-16β-乙酰氧基-海参烷-9-烯-22,25-环氧-3β,12α,17α-三醇,命名为花刺参苷B(variegatuside B,2)。结论化合物1和2均为新化合物。