天麻钩藤饮控释胶囊的制备及体外释放度试验

目的:对天麻钩藤饮控释胶囊的制备工艺、体外释放度进行研究,为天麻钩藤饮控释胶囊的新药申报提供部分实验数据资料。方法:以乙基纤维素为辅料,制备天麻钩藤饮控释胶囊。以体外释放性能和成品外观性状为综合指标,对乙基纤维素乙醇液的浓度进行优选,优选出最佳处方和制备工艺。结果:验证实验结果表明,天麻钩藤饮控释胶囊释药基本达到零级过程,通过零级方程拟合,r=0.9991。加入天麻钩藤饮浸膏粉作为速释部分可提高控释胶囊的释药速率。结论:该控释胶囊的制备方法可行,达到了控释目的。     

Tomentodione E,a new sec-pentyl syncarpic acid-based meroterpenoid from the leaves of Rhodomyrtus tomentosa

A new meroterpenoid, tomentodione E (1), along with four known ones (2–5) were isolated from the leaves of Rhodomyrtus tomentosa. Their structures were elucidated based on extensive spectroscopic data as well as computational methods. Compound 1 represents the first example of meroterpenoid possessing a sec-pentyl syncarpic acid motif coupled with a caryophyllene. Compounds 1–4 were evaluated for their in vitro antiviral activity against respiratory syncytial virus (RSV) with cytopathic effect (CPE) reduction assay, and 2 showed potent in vitro anti-RSV effect.     

Influence of Tilia tomentosa Moench Extract on Mouse Small Intestine Neuromuscular Contractility

Functional gastrointestinal disorders (FGIDs) are characterized by abdominal pain, bloating and bowel disturbances. FGID therapy is primarily symptomatic, including treatment with herbal remedies. Flower extract of Tilia tomentosa Moench (TtM) is occasionally used as an anti-spasmodic in popular medicine. Since its effect on intestinal response is unknown, we evaluated the influence of TtM extract on small intestine contractility. Ileal preparations from C57BL/6J mice were mounted in organ baths to assess changes in muscle tension, following addition of TtM extract (0.5–36 μg/mL) or a vehicle (ethanol). Changes in contractile response to receptor- and non-receptor-mediated stimuli were assessed in ileal preparations pretreated with 12 μg/mL TtM. Alterations in the enteric nervous system neuroglial network were analyzed by confocal immunofluorescence. Increasing addition of TtM induced a marked relaxation in ileal specimens compared to the vehicle. Pretreatment with TtM affected cholinergic and tachykininergic neuromuscular contractions as well as K⁺-induced smooth muscle depolarization. Following incubation with TtM, a significant reduction in non-adrenergic non-cholinergic-mediated relaxation sensitive to Nω-Nitro-L-arginine methyl ester hydrochloride (pan-nitric oxide synthase inhibitor) was found. In vitro incubation of intestinal specimens with TtM did not affect the myenteric plexus neuroglial network. Our findings show that TtM-induced intestinal relaxation is mediated by nitric oxide pathways, providing a pharmacological basis for the use of TtM in FGIDs.     

鼻冠状位CT研究钩突解剖变异与慢性鼻窦炎的关系

目的通过鼻冠状位CT对钩突解剖变异与慢性鼻窦炎的关系进行分析研究。方法此次研究,选取我院接受CT检查的130例患者,检查时间为2017年9月至2019年11月期间,检查结果无鼻窦炎患者为对照组,慢性鼻窦炎患者为研究组,对比分析两组患者检查后钩突解剖变异与鼻窦炎的关系。结果研究组患者的钩突高度、钩突角度以及筛漏斗宽度明显高于对照组患者的各项数据(P<0.05);钩突头端附着类型与鼻窦炎关系分析中,研究组患者与对照组患者之间纸样板型、中鼻甲型发生率相对比有明显差异(P<0.05),研究组与对照组患者之间分叉型、筛顶型发生率相对比无明显差异(P>0.05);钩突其他解剖变异与鼻窦炎关系中,研究组与对照组患者之间钩突偏曲、以及肥大两项具有较为明显的差异(P<0.05),且研究组与对照组患者之间钩突气化、以及钩突缺失发生率相对比无明显差异(P>0.05)。结论数据中表明,慢性鼻窦炎患者出现钩突解剖变异几率明显较高,其中较为常见的包括纸样板型、中鼻甲型,钩突解剖变异与慢性鼻窦炎的关系较为紧密,对于慢性鼻窦炎患者治疗有着极为重要的临床意义。     

化橘红rDNA ITS序列特征的初步分析

目的:研究化橘红及其近缘种rDNA ITS区碱基序列的特征及差异。方法:利用PCR产物直接测序,并作序列变异分析。结果:5个植物样品的rDNA ITS序列长度为675bp-683bp,其中5.8S rDNA长度为165bp,编码区较保守。基于K2P参数遗传距离模型构建的化橘红与沙田柚、柑橘rDNA ITS序列遗传距离分别为1.05和2.29。结论:化橘红与其近缘品种rDNA ITS序列有明显的差异。     

Mixtures of Uncaria and Tabebuia extracts are potentially chemopreventive in CBA/Ca mice: a long‐term experiment

A long‐term experimental animal model was developed by our research group for the evaluation of potential chemopreventive effects. The inhibitory effects of agents on carcinogen (7,12‐dimethylbenz[a]anthracene (DMBA) induced molecular epidemiological biomarkers, in this case the expression of key onco/suppressor genes were investigated. The expression pattern of c‐myc, Ha‐ras, Bcl‐2, K‐ras protooncogene and p53 tumour suppressor gene were studied to elucidate early carcinogenic and potential chemopreventive effects. The consumption of so‐called Claw of Dragon tea (CoD? tea) containing the bark of Uncaria guianensis, Cat's Claw (Uncaria sp. U. tomentosa) and Palmer trumpet‐tree (Tabebuia sp. T. avellanedae) was able to decrease the DMBA‐induced onco/suppressor gene overexpression in a short‐term animal experiment. In a following study CBA/Ca mice were treated with 20 mg/kg bw DMBA intraperitoneally (i.p.) and the expression patterns of onco/suppressor genes were examined at several time intervals. According to the examined gene expression patterns in this long‐term experiment the chemopreventive effect of CoD? tea consumption could be confirmed. Copyright © 2010 John Wiley & Sons, Ltd.     

毛泡桐叶化学成分研究

目的:对毛泡桐叶的化学成分进行分离与鉴定。方法:利用硅胶色谱柱层析进行分离纯化,通过波谱分析鉴定其结构。结果:从毛泡桐叶的乙酸乙酯部分分离得到10个化合物,分别鉴定为:洋芹素(Ⅰ)、木樨草素(Ⅱ)、高北美圣草素(Ⅲ)、3α-羟基-乌苏酸(Ⅳ)、19α-羟基-乌苏酸(坡模酸)(Ⅴ)、2α,3α-二羟基-12烯-28-乌苏酸(Ⅵ)、乌苏酸(Ⅶ)2、α-羟基-齐墩果酸(山楂酸)(Ⅷ)、胡萝卜苷(Ⅸ)、β-谷甾醇(Ⅹ)。结论:化合物Ⅰ～Ⅹ为首次从毛泡桐叶中分离得到。     

不同产地及不同采收期钩藤中异钩藤碱的含量分析

目的：研究不同产地及不同采收期钩藤中异钩藤碱的含量。方法应用HPLC测定8个不同产地及不同采收期钩藤中异钩藤碱含量,采用Waters Symmetry C18色谱柱(250 mm34.6 mm,5μm),流动相为甲醇-0.01 mol/L醋酸铵缓冲液(pH 8.0)(60∶40),柱温25℃,进样体积20μl,流速1.0 ml/min,检测波长246 nm。结果不同产地及不同采收期的钩藤中异钩藤碱含量不同,其中含量最高的地区为宁明县;含量最高的采收时间为每年9月至次年2月。结论不同产地钩藤药材异钩藤碱的含量不同,每年9月至次年2月采收的钩藤中异钩藤碱含量较高。     

罗布麻降压片的薄层色谱鉴别

目的:鉴别罗布麻降压片中的罗布麻、钩藤、泽泻。方法:采用薄层色谱法。结果:鉴别方法简便迅速。结论:建立的方法可有效地控制本制剂质量。     

高效液相色谱法测定毛泡桐花中麦角甾苷（毛蕊花糖苷）的含量

目的:建立毛泡桐花中麦角甾苷(毛蕊花糖苷)的高效液相色谱分析方法。方法:采用Kromasil C18色谱柱(250mm×4.6mm,5μm),以乙腈-1％冰醋酸溶液(20:80)为流动相,流速1.0 mL·min-1,检测波长331mm,在10 min内分离检测该化合物。结果:麦角甾苷在0.006-10μg范围内呈良好的线性关系(r=0.9999),最低检出限0.002μg(S/N=3);日内和日间精密度RSD分别为0.6％和2.9％(n=5);加样回收率为95.8％-103.1％。结论:所建立的方法简便、快速、准确,可用于毛泡桐花的质量控制,并为泡桐属其它植物中苯丙素苷类化合物的分离分析提供一定的参考。