Survivin与p53的组合在卵巢上皮性癌早期诊断中的意义

目的 探讨Survivin与p53在卵巢上皮性癌诊断中的意义。方法 采用免疫组化方法检测Sur—vivin及p53蛋白在57例卵巢上皮性囊腺癌和19例卵巢上皮性良性肿瘤组织中的表达。结果 Survivin基因在卵巢上皮性良性肿瘤细胞内无表达,在恶性肿瘤的表达率为42．1％。p53在卵巢上皮性良、恶性肿瘤细胞中的表达率分别为26．3％、56．1％。Survivin基因表达与临床分期、组织学类型无相关性（P〉0．05）,与良、恶性有关（P〈0．05）。p53蛋白的表达与卵巢上皮性癌的临床分期以及良、恶性有相关性。p53蛋白与Survivin的阳性表达率有显著性差异（P〈0．01）。结论 Survivin基因在卵巢上皮性良性与恶性肿瘤的表达有差异,与临床分期、组织学类型无关。p53蛋白的表达与临床分期及良、恶性均有相关性。Survivin与p53在卵巢上皮性癌的表达有显著相关性。     

槲皮素对人肾癌细胞增殖、凋亡及stat3、survivin表达的影响

目的探讨槲皮素对人肾癌细胞增殖、凋亡及stat3、survivin表达的影响。方法在肾癌细胞体外培养的基础上,根据不同浓度槲皮素（20μmol／L、40μmol／L和80μmol／L）分别3组,即低、中、高浓度干预组;另设空白对照组。观察不同浓度的槲皮素对肾癌细胞增殖、凋亡及stat3、survivin的mRNA和蛋白表达的影响。采用MTT法检测槲皮素对细胞的毒性作用。用流式细胞仪检测细胞凋亡。Real Time PCR检测stag和survivin的mRNA表达,采用Western blot检测star3和survivin的蛋白表达。结果随作用时间延长和浓度增高,槲皮素对细胞增殖的抑制作用增强。各槲皮素干预组与空白对照组比较均具有统计学差异（P〈0．05）。在槲皮素作用48h时检测,槲皮素（20μmol／L）组细胞早期凋亡率为20．17％、槲皮素（40μmol／L）组细胞早期凋亡率为61．93％、槲皮素（80μmol／L）组细胞早期凋亡率为82．24％。各槲皮素干预组与空白对照组比较差异均有统计学意义（P〈0．05）。stat3与survivin mRNA之间呈直线相关关系（r=0．697,P=0．002）,且磷酸化staG（p-staG）和survivin蛋白也呈直线相关关系（r=0．748,P=0．003）。结论槲皮素可抑制肾癌细胞的增殖,诱导细胞凋亡,下调stat3和survivin的mRNA和蛋白表达。     

Sulforaphane retards the growth of UM-UC-3 xenographs,induces apoptosis,and reduces survivin in athymic mice

Sulforaphane (SFN), an isothiocyanate that exists exclusively in cruciferous vegetables, may be the most promising preventive agent for bladder cancer (BC) to date. We previously observed that SFN dramatically inhibits human BC T24 cells in vitro. Our hypothesis is that SFN may attenuate BC growth. To test our hypothesis, we investigated the effect of SFN on human BC UM-UC-3 cell xenografts implanted into athymic mice. Sulforaphane extract was routinely prepared in our laboratory, and its content was measured with high-performance liquid chromatography. Athymic mice were injected subcutaneously with a UM-UC-3 cell suspension (2.0 × 10⁶ cells/200 μL per mouse) and randomly divided into 2 groups. The positive control group was orally gavaged with water, and the treatment group was orally administered SFN from broccoli sprout (12 mg/kg body weight) for 5 weeks. At the end of the experiment, tumor tissues were harvested and processed for hematoxylin and eosin staining and immunohistochemistry. The average tumor volume decreased from 4.1 ± 1.67 cm³ in the positive control mice to 1.5 ± 0.72 cm³ in the SFN-treated mice, evidencing an inhibitory rate of 63%. The SFN extract also reduced the appearance of tumors, including karyopyknosis and angiogenesis. Sulforaphane extract induced caspase 3 and cytochrome c expression but reduced the expression of survivin. Sulforaphane extract retards the growth of UM-UC-3 xenografts in vivo, confirming its future potential in BC therapy.     

保定市市直女工健康状况调查分析

目的:通过群体调查了解市直女工的工作、生活和健康状况,为制定干预措施提供依据。方法:2005年4月~6月调查了保定市15家市直单位的778例妇女。结果:月工资在500~1 000元的占66.2%,>1 000元占27%,居住面积>100 m2占54%,家庭常住人口3人者占73%,干部和公务员占70%,生存环境(饮食、睡眠、工作)舒适者占42.2%,身体超重和肥胖者占27%。现患病率达77.5%,依次是眼科、妇科、乳腺、骨关节、消化系统、心血管系统疾病;既往患病率为46%,肿瘤居首位、其次是妇科、心血管、内分泌、肾病和肝胆疾病。结论:市直女工的健康状况需要多部门关注和重视,医疗保健部门要加大健康教育力度,普及防病和心理保健知识,改变她们不良的工作和生活习惯,重视每年的妇女病普查普治,提高妇女的健康水平。     

Recent advances in sepsis and septic shock

Sepsis remains a common problem in all age groups. Recently surviving sepsis campaign has taken up a worldwide initiative by publishing international guidelines 2008 with a hope to disseminate information regarding management of sepsis for all age groups. This article presents a review of recent advances as they apply to pediatric age group supported by the available evidence with reference to standard definitions of pediatric sepsis and septic shock and management in the emergency room and pediatric intensive care unit.     

健脾法延长胃癌患者生存期之探讨

探讨进展期胃癌患者脾虚状况与其生存期之间的关系。按脾虚主要症状半定量分级标准 ,将 6 1例进展期胃癌患者分为轻度脾虚组和中重度脾虚组 ,比较两组患者生存期 ,并通过对不同组别的毕格犬生存时间的比较 ,检测健脾法对胃癌术后毕格犬生存时间的影响。结果 ,中重度脾虚组在生存时间及脾虚主要症状半定量积分上与轻度脾虚组相比有极其显著差异 ,且生存时间及脾虚主要症状半定量积分差值呈负相关。提示脾虚程度越严重则患者生存时间越短。这一结果同样为毕格犬模拟临床分组实验所证实。中医理论认为 ,“脾为后天之本” ,可以认为 ,观察患者的脾虚状况在一定程度上可以对患者的生存时间及预后情况有一个较准确的估计 ,值得扩大样本数做进一步的研究。