七氟醚和安氟醚对心肌缺血再灌注损伤的保护作用

目的：从细胞生化方面来探讨七氟醚和安氟醚对心肌缺血再灌注损伤的影响。方法：采用Ｌａｎｇｅｎｄｏｒｆ离体大鼠心脏模型，将３０只大鼠随机分成三组：对照组、七氟醚组和安氟醚组，每组各１０只。全心缺血３０分钟，再灌注３０分钟。结果：缺血前给予１ＭＡＣ的七氟醚或安氟醚可以明显减少再灌注冠脉流出液中乳酸脱氢酶和蛋白的释放量，降低心肌细胞内钙聚集，保护了心肌ＳＯＤ的活力。结论：七氟醚和安氟醚对缺血再灌注心肌有显著的保护作用。     

中药对实验性玻璃体出血的作用观察

观察中药对实验性玻璃体出血的治疗作用。用兔自体血造成玻璃体出血模型，并分眼血宁治疗组，空白对照组，模型对照组及尿激酶对照组，１０天后观察玻璃体Ｆｅ６２＋，蛋白浓度和超氧化物歧化酶活性的变化。结果；玻璃体出血时玻璃体内Ｆｅ＾２＋和蛋白浓度升高，ＳＯＤ活性下降；治疗组Ｆｅ＾２＋浓度明显降低，ＳＯＤ活性增强。结论：眼血宁不仅能促进玻璃体出血吸收，同时对出血造成的玻璃体组织结构损害有一定促进恢复作用。     

麝香保心丸治疗冠心病心绞痛35例

中西医结合治疗慢性高原性心脏病充血性心力衰竭１５例张德才，次仁布芷１９９１年１月～１９９６年１０月，我院采用中西医结合治疗慢性高原性心脏病充血性心力衰竭１５例，疗效满意，现报告如下。临床资料诊断标准符合我国高原病命名，分型及诊断标准［西藏医药杂志１９...     

普乐林治疗老年冠心病心绞痛30例

体外反搏及加用补阳还五汤对冠心病患者血清载脂蛋白的影响叶小卫，侯灿，郑振声本研究通过观察体外反搏及加用补阳还五汤治疗对冠心病患者载脂蛋白（ａｐｏ）Ｂ１００（ａｐｏＢ１００）、ａｐｏＡ１及ａｐｏＢ１００／ａｐｏＡ１比值的影响，探讨了两种治疗的作用及机制...     

Linoleic acid and tumor necrosis factor-α increase manganese superoxide dismutase activity in intestinal cells

Manganese superoxide dismutase (MnSOD) protects mitochondria from oxidative damage. Alterations in the regulation of MnSOD plays an important role in the development of many types of cancer. Activity of this enzyme is induced by inflammatory cytokines and other conditions that increase oxygen radical production. High levels of dietary lipid have been shown to decrease MnSOD activity. This study was designed to define the effect of various type of fatty acids on MnSOD activity and MnSOD induction. IEC-6 cells were treated with 40 μmol/l of either linoleic acid (LA), eicosapentaenoic acid (EPA), or oleic acid (OA) in the presence or absence of 10 ng/ml tumor necrosis factor-α (TNF-α). Fatty acid supplementation increased MnSOD activity. MnSOD activity was greater in the LA group than in the EPA or OA groups. TNF-α induced MnSOD activity equally in all fatty acid-supplemented groups. High levels of MnSOD activity may be an indicator of chronic inflammation resulting from fatty acid, particularly LA, supplementation.     

富锗灵芝菌丝体氨基酸及超氧化物歧化酶含量的测定

目的:测定富锗灵芝菌丝体氨基酸及超氧化物歧化酶(SOD)的含量,探讨培养基中GeO2的最适加量。方法:高效液相色谱仪分析氨基酸,氯化硝基四氮唑蓝(NBT)光化还原法测定SOD的含量。结果:培养基中添加400 mg.L-1的GeO2,灵芝菌丝体中氨基酸含量比对照提高54.34%,SOD提高16.73%。结论:为富锗灵芝菌丝体的应用奠定基础。     

三保心对大鼠心肌缺血-再灌注损伤保护作用及其对体内外血栓形成影响的实验研究

目的：探讨三保心中药复方(三保心)对大鼠心肌缺血-再灌注损伤的保护作用及对体外血栓形成的影响。方法：结扎大鼠左冠状动脉前降支40min后再灌注180min。观察三保心对心肌缺血再灌注损伤大鼠超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和一氧化氮(NO)含量的影响。采用chandler法造成体外血栓和电刺激颈总动脉形成体内血栓，观察三保心对体内外血栓形成的影响。结果：三保心可显著提高心肌缺血-再灌注损伤大鼠血清中SOD活性，降低MDA含量，同时明显升高NO含量。三保心高剂量组明显延长大鼠体内血栓形成时间。各剂量组均能显著抑制大鼠体外血栓的形成。结论：三保心对缺血再灌注心肌损伤有一定的保护作用，其作用机制可能与扩张血管、提高氧自由基清除酶活性、抑制脂质过氧化反应有关。且具有抑制体内外血栓形成的作用。     

A novel effect of an opioid receptor antagonist, naloxone, on the production of reactive oxygen species by microglia: a study by electron paramagnetic resonance spectroscopy

Microglia as the first line of defensive cells in the brain produce free radicals including superoxide and nitric oxide (NO), contributing to neurodegeneration. An opioid receptor antagonist, naloxone, has been considered pharmacologically beneficial to endotoxin shock, experimental cerebral ischemia, and spinal cord injury. However, the mechanisms underlying these beneficial effects of naloxone are still not clear. This study explores the effects of naloxone on the production of superoxide and NO by the murine microglial cell line, BV2, stimulated with lipopolysaccharide (LPS) as measured by electron paramagnetic resonance (EPR). The production of superoxide triggered by phobol-12-myristate-13-acetate (PMA) resulted in superoxide dismutase (SOD)-inhibitable, catalase-uninhibitable 5,5-dimethyl-1-pyrroline N-oxide (DMPO) hydroxyl radical adduct formation. LPS enhanced the production of superoxide and triggered the formation of non-heme iron-nitrosyl complex. Cells pre-treated with naloxone showed significant reduction of superoxide production by 35%. However, it could not significantly reduce the formation of non-heme iron-nitrosyl complex and nitrite. Taken together, the results expand our understanding of the neuroprotective effects of naloxone as it decreases superoxide production by microglia.     

维生素A干预对大鼠抗氧化能力及细胞膜流动性影响的研究

目的　通过对大鼠补充不同剂量维生素A(VA)观察机体抗氧化能力及对红细胞膜流动性的影响 ,了解抗氧化活性的最佳剂量。方法　将Wistar大鼠随机分为 4组 ,分别为维生素A缺乏组 (VA1组 )补充1 1 4 3(VA2组 )、4 2 86 (VA3组 )和 1 4 2 86 μgRE·kg- 1 ·d- 1 (VA4组 )维生素A三个剂量组。测定各组大鼠血浆VA含量、SOD、MDA和GSH Px的含量和活性 ,用荧光偏振度P值和微粘度 η值评价红细胞膜的流动性。 结果　各组血浆中VA水平随补充剂量的增加而增加。血浆中SOD、GSH -Px和MDA的结果显示 ,补充剂量为VA2、VA3组SOD的水平较VA缺乏组和VA4组明显偏低 (P <0 0 1 )。VA3组的MDA较其它 3个剂量组明显下降 (P <0 0 1 )。VA4组的SOD、MDA含量明显高于其它三个剂量组 (P <0 0 1 )。VA3组血浆中GSH -Px的活性明显升高 ,P <0 0 1。细胞膜流动性的结果显示 :VA3组P值最小 (P <0 0 1 ) ;VA4组P值明显增高 (P<0 0 1 )。微粘度 η在VA3组与VA缺乏组和VA2组之间未见明显差异 ,与VA4组相比明显下降 (P <0 0 1 )。结论　维生素A补充剂量为 4 2 86 μgRE·kg- 1 ·d- 1 时 ,能够发挥较好抗氧化作用 ,红细胞膜的流动性明显增加 ,过量摄入维生素A可造成机体的中毒反应 ,增加机体的氧化损伤     

高氧诱导慢性肺疾病早产鼠肺组织中氧化及抗氧化系统的动态研究

目的　探讨肺组织中氧化及抗氧化系统在高氧致早产儿慢性肺疾病 (CLD)中的动态变化规律。　方法　采用高浓度氧致早产鼠CLD模型为研究对象 ,分为实验组和对照组 ,各 4 0只。应用分光光度计比色法分别测定肺组织中脂质过氧化产物丙二醛 (MDA)的含量及超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶 (GSH Px)和过氧化氢酶 (CAT)的活性。　结果　实验组和对照组各时间点肺组织中SOD、GSH Px及CAT的活性差异均无显著性 (P >0 .0 5 ) ;而实验组MDA水平早期即明显升高 [(5 5 .92± 5 .5 3)nmol/mg肺组织 ,P <0 .0 1],7d达高峰 [(94 .30± 12 .4 0 )nmol/mg肺组织 ,P <0 .0 0 1],持续 1周后逐渐下降 ,2 1d时仍高于正常水平 [(47.95± 7.4 8)nmol/mg肺组织 ,P <0 .0 1]。　结论　肺组织中抗氧化酶系统的活性不足及氧自由基损伤始终伴随高氧致CLD发生、发展的全过程。