五子衍宗丸对少弱精子症模型大鼠睾丸组织Catsper1蛋白及其mRNA表达影响

目的 通过检测大鼠睾丸组织Catsper1蛋白及其mRNA表达水平,探讨五子衍宗丸治疗少弱精子症的作用机制。方法 将体质量200～220 g的雄性SD大鼠随机分成正常组,模型组,生精胶囊组,五子衍宗丸低剂量、中剂量、高剂量组。采用连续灌服雷公藤多苷8周的方法复制少弱精子症模型,模型复制成功后连续给药4周。采用Western blot法测定Catsper1蛋白含量,采用荧光定量PCR法测定Catsper1 mRNA表达水平,采用酶联免疫吸附试验检测睾丸组织中cAMP含量。结果 生精胶囊和高剂量五子衍宗丸能明显提高模型大鼠睾丸组织中降低的cAMP含量以及升高睾丸组织中降低的Catsper1蛋白及其mRNA表达水平（P<0.05）。结论 促进Catsper1蛋白表达并参与cAMP-Ca2+正反馈可能是五子衍宗丸提高精子质量的机制之一。     

六味地黄滴心丸制备工艺的研究

目的:优选六味地黄滴心丸的制备工艺。方法:滴心丸的制备过程分二步:第一步以牡丹皮萃取物、泽泻萃取物为主药制成滴丸,通过单因素试验、正交试验,以多指标综合评分法优选制备条件。第二步以滴丸为母核,采取泛制方法,取混合好的六味地黄方药粉、辅料等包裹于滴丸母核上,成型,盖面,干燥,整丸,选丸,包衣,制成六味地黄滴心丸。结果:滴丸母核制备的最佳工艺条件为:主药与基质配比不得低于1∶2;基质应采用PEG10000与PEG6000混合的二种基质,比例为1∶10;冷凝液选择二甲基硅油,温度为10℃;滴头温度85℃;滴距5cm。泛制法制丸时最适宜的黏合剂为:甘油:95%乙醇=1∶9。结论:用该方法制备的六味地黄滴心丸,质量可控,方法可靠,易于崩解,可防止挥发性及脂溶性物质丢失,适合于含挥发性及脂溶性物质的中药复方的剂型制备。     

通脉地仙丸治疗慢性脑供血不足40例

目的:观察通脉地仙丸治疗慢性脑供血不足(CCC I)的疗效。方法:80例确诊患者随机分为两组,治疗组40例口服通脉地仙丸;对照组40例口服辛伐他汀、氟桂利嗪片、拜阿斯匹林。记录服药前后两组患者脑血管多普勒超声血流图(TCD),头痛、眩晕、头重、耳鸣、失眠、健忘等临床症状,血清SOD、MDA、CRP,血流变和血脂的情况,两组均3个月为1个疗程,1个疗程后统计疗效。结果:治疗组患者脑血管多普勒超声血流图(TCD)指标改善,提高SOD,降低MDA、CRP,头痛、眩晕、头重、耳鸣、失眠、健忘症状改善,血流变、血脂指标改善,与组内治疗前比较(P<0.05,P<0.01),与组间治疗后比较(P>0.05)。结论:通脉地仙丸治疗CCC I疗效明显,且无任何毒副作用。     

加味鳖甲煎丸对白蛋白所致免疫性肝纤维化大鼠病理模型的影响

目的　对加味鳖甲煎丸对白蛋白所致免疫性肝纤维化大鼠的病理和病变程度进行定量分析。     

安宫牛黄丸对自发性高血压大鼠急性脑出血后脑水肿及神经功能缺损的影响

目的:观察安宫牛黄丸对自发性高血压大鼠脑出血后脑组织含水量、脑系数以及神经功能缺损的影响。方法:将自发性高血压大鼠随机分为5组:空白对照组、模型组、西药治疗组、中药治疗组、中西药结合治疗组,于6、24、48、72h观察大鼠神经行为学评分,测量脑组织含水量与脑系数。结果:模型组大鼠脑组织含水量、脑系数及行为学评分较空白对照组高(P<0.05),3个治疗组脑组织含水量、脑系数及行为学评分低于模型组(P<0.05),中西结合治疗组脑组织含水量、脑系数及行为学评分明显低于中药治疗组及西药治疗组(P<0.05),中药治疗组与西药中药治疗组与西药治疗组间相比差异无显著性。结论:安宫牛黄丸能够有效减轻脑出血后脑组织含水量、减少脑系数并改善神经功能缺损症状。     

软肝汤治疗慢性肝炎后肝纤维化的临床观察

观察软肝汤治疗慢性肝炎后肝纤维化的临床疗效。将60例患者随机分成软肝汤治疗组和人参鳖甲煎丸对照组各30例,观察其对症状,体征,肝功能,肝纤维化指标的改善作用,疗程6个月,结果：治疗组对症状体征有明显改善,其中神疲乏力症状的改善与对照组相比有显著差异P＜0．01,对肝功能指标G,γ－球蛋白的改善治疗前后有显著差异P＜0．05,γ－球蛋白的改善与对照组相比也有显著差异P＜0．01,对肝纤维化指标HA,IVC,PIIIP,LN的改善治疗前后均有显著差异P＜0．01,与对照组相比HA,LN,IVC有显著差异P＜0．05,总有效率治疗组为83．33％, 对照组为73．33％（P＜0．05）,治疗组显效率33．33％,对照组23．33％（P＜0．01）,提示软肝汤对治疗乙型病毒性肝炎肝纤维化,早期肝硬化有较好疗效。     

温养化痰方治疗60例支气管哮喘慢性持续期患者的临床观察

目的:观察温养化痰方治疗支气管哮喘慢性持续期(肺肾气虚、寒痰证)患者的临床疗效,总结周仲瑛教授治疗支气管哮喘慢性持续期的临床经验。方法:对符合纳入条件的60例患者给予温养化痰方,10g/次,2次/d。共服用24周,对于急性发作患者可给予适当的西医治疗。在治疗前、治疗后12周、治疗后24周对临床疗效进行评定。结果:患者临床痊愈17例,显效34例,有效9例,无效0例。受试者服用温养化痰方0周、服用后12周、服用后24周,患者的证候总积分、主要症状积分有持续下降趋势,哮喘控制测试评分有持续上升趋势,不同时间点之间比较差异具有统计学意义(P<0.01)。结论:温养化痰方治疗支气管哮喘慢性持续期(肺肾气虚、寒痰证)患者具有良好的临床疗效,值得进一步研究和探索。     

Ending prematurely a weight loss programme: the impact of child and family characteristics

The present study evaluates the pre‐treatment child and family characteristics as well as the parental motives and experienced barriers when ending prematurely a weight loss programme for their obese child, in accordance with the ‘barriers‐to‐treatment model’ and subsequent research on dimensions underlying treatment termination decision. Treatment seeking youngsters (n = 72) with a mean of 10.46 years (SD = 2.56) were followed over a 1‐year period. Dropout, child and family characteristics were measured both during assessment and treatment. The ‘barriers‐to‐treatment’ data were collected at the 1‐year follow‐up via mail (response rate = 65%; n = 47). Results showed that dropout could be successfully predicted at intake by age, psychopathology in the child and motivation of the parents. Results indicated further that there are few differences in total barriers score between completers and non‐completers. Item content analysis revealed two themes that were more often reported by non‐completers (appointment arrangements and perceived relevance of treatment). The hypothesis that non‐completers (compared to completers) experienced more barriers that burden their willingness to continue a weight loss programme could not be confirmed. Besides demographic characteristics, specifically motivation of the parents at intake seems an important area for future research. Copyright © 2009 John Wiley & Sons, Ltd. Key Practitioner Message: ? Families non‐completing a treatment suffer from a greater burden due to psychological problems in their child. ? Regarding the decision to start a treatment, specifically pre‐treatment, motivation and expectations of parents will play a crucial role. ? 47% of the families ended prematurely while their children still suffer from overwight, fueling our assumption that dropping out in treatment is a serious problem and comprises a substantial group of people not studied yet in detail. ? The hypothesis that non‐completers (compared to completers) experienced more barriers that burden their willingness to continue a weight loss program could not be confirmed.     

高效液相色谱法测定补益资生丸中柚皮苷的含量

目的建立补益资生丸中柚皮苷的高效液相色谱法。方法用十八烷基硅烷键合硅胶为填充剂;甲醇-1%醋酸（33∶67）为流动相;检测波长为283nm。结果柚皮苷进样量在0.04096～1.0240μg范围内呈良好的线性关系,回归方程Y=1786.06X-1.93,r=0.9999;平均回收率为99.64%。结论本法精密准确,稳定耐用,适用于补益资生丸的含量测定。