丹参对大鼠烫伤早期肝脏超微结构改变的影响

目的:观察大鼠严重烫伤早期肝脏超微结构的变化及丹参的影响作用。方法:建立30％Ⅲ度烫伤模型,88只Wistar大鼠随机分为正常对照组(A组,8只)、平衡液复苏组(B组,40只)和丹参治疗组(C组, 40只),B、C两组烫伤后立即按Parkland公式腹腔注射平衡液120ml/kg,C组在同样治疗的基础上腹腔注射丹参注射液3ml/kg,B、C两组分别于伤后3,6,12,24,48h各时相点任选两只动物肝脏,取其左外叶分两份标本分别置于95％酒精和2．5％戊二醛小瓶中固定,光镜、电镜下观察肝组织形态学变化。结果:肝组织形态学和超微结构改变在伤后6h开始出现,并进行性加重,48h最明显;丹参治疗组肝组织病理改变轻于复苏组。结论:严重烫伤早期肝组织超微结构即可发生改变,丹参治疗后肝组织超微结构改变减轻。     

丹参多酚酸治疗心绞痛患者的临床疗效及对血小板功能的影响

目的　探讨丹参多酚酸盐治疗稳定性心绞痛的临床疗效以及对血小板功能的影响。方法　将稳定型心绞痛患者 5 6例随机分为丹参多酚酸高剂量组、低剂量组和丹参对照组 ,均治疗 14d ,观察3组的症状缓解、平板运动试验改善情况 ,以及比浊法检测的血小板聚集率、全血法流式细胞术检测的P选择素变化情况。结果　丹参多酚酸组在症状缓解、平板运动试验改善方面与丹参组相近 ,在降低血小板聚集率和P选择素方面优于丹参组。结论　丹参多酚酸能明显抑制血小板的聚集和活化 ,改善心绞痛症状及平板运动试验心电图ST T改变     

Beneficial effects of Salvia miltiorrhiza in the healing of burn wounds: an experimental study in rats

Introduction: Burn healing is a complicated process and very few treatments can positively alter its effects. The aim of this study was to investigate the effects Salvia miltiorrhiza (SM -Danshen), a traditional Chinese medicine, on burn wound healing.

Material and methods: Twenty rats were included in this study and divided into two groups. 3?×?2?cm wide burn areas were created in the dorsal skin of all the animals with thermal contact. Intraoral 1?ml/day saline and 1?g/kg/day SM were given in control and experiment groups, respectively. Fourteen days following the burn injury burn zones were evaluated with indocyanine green-SPY imaging device, and multiple samples were collected for histopathological evaluation. Standard photographs were taken for the evaluation of necrotic skin areas.

Results: Neovascularization was increased in the SM group when compared with the control group (p?=?0.0406). SPY studies revealed a meaningful increase in the tissue perfusion in the SM group (p?=?0.0286). The average amount of necrotic area in the control and experiment group on the postoperative 14th day was 71.6% (±16.51) and 42.5% (±10.64) respectively (p?=?0.0002).

Conclusion: Our study shows that SM can decrease the amount of necrosis in burn wounds by increasing tissue perfusion and neovascularization.     

大黄丹参对高脂饮食诱发的胰腺纤维化大鼠影响的研究

目的:研究大黄丹参对高脂饮食诱导的胰腺纤维化大鼠氧化应激、内质网应激相关因子的影响。方法:将40只雄性清洁级SD大鼠,采用随机数表法均分成对照组、模型组、大黄丹参低、中、高剂量组,每组8只;对照组喂以普通饲料,其余4组均喂以高脂饲料连续10周建立胰腺纤维化模型。造模同时,各组以相应药物灌胃,对照组与模型组生理盐水灌胃,...     

知母皂苷对衰老大鼠脑乙酰胆碱受体的调节作用

目的：研究知母皂苷（SAaB)对衰老大鼠脑乙酰胆碱受体的影响。方法：采用放射受体配体结合实验,测定大鼠脑皮层M,N受体的密度及亲和力。结果：与正常成年大鼠比较,衰老大鼠脑皮层M受体的密度和亲和力我明显变化,而脑N受体的密度显减少;SAaB能剂量依赖性地增加衰老大鼠脑N受体的数目,对MC受体无明显影响。结论;SAaB对衰老大鼠脑N受体有上调作用。     

肢体缺血再灌注致远处器官损伤及丹参、甘露醇的保护作用

目的　通过动物实验观察丹参和甘露醇对肢体缺血再灌注所致心、肝、肾、胰损伤的保护作用。方法　 15 0只Wis tar大鼠随机分成正常对照组 ,缺血 2h ,缺血 3h ,缺血 3h分别用丹参和甘露醇共 5个组。实验组于缺血、再灌注 1,2 ,3和 2 4h取材光镜对比观察。结果　肢体缺血和再灌注后 ,实验组心、肝、肾、胰毛细血管扩张、充血、中性白细胞浸润、细胞变性、组织损伤。用丹参和甘露醇组的病变明显轻于未用药组。结论　丹参和甘露醇都有减轻肢体缺血再灌注所致远处器官损伤作用。     

Co-administration of Salvia miltiorrhiza and verapamil inhibits detrimental effects of torsion/detorsion on testicular tissue in rats

Testicular torsion/detorsion is one of the important emergencies that requires fast surgical intervention. This study aimed to investigate the effects of Salvia miltiorrhiza hydroalcoholic extract combined with verapamil on testicular ischaemia/reperfusion damage in Wistar albino rats. All animals were distributed in 3 groups (n = 8), including the sham-operated group, torsion/detorsion (TD) group and torsion/detorsion + pretreatment with 200 mg/kg Salvia miltiorrhiza extract combined with 0.3 mg/kg verapamil (SMV) group. Oxidative stress biomarkers (MDA, GPx, CAT and TAC) both in plasma and testicular tissue, sperm parameters (motility, vitality, concentration and morphology) and histopathological parameters (MSTD, GECT, Johnson's score, Cosentino's score and testicular cell thickness) were assessed in all groups. Ischaemia/reperfusion significantly increased MDA and decreased GPx, CAT and TAC levels (p < .05). Pretreatment with SMV significantly increased GPx, CAT and TAC levels (p < .05). SMV group increased progressive sperm motility and vitality and reduced non-progressive motility of spermatozoon (p < .05). Testicular torsion significantly decreased all histopathological parameters compared to the sham group (p < .05). SMV pretreatment remarkably increased MSTD, GECT and Cosentino's score in comparison with the TD group (p < .05). A combination of Salvia miltiorrhiza with verapamil could reduce damages triggered by testicular torsion detorsion and improve sperm functionality parameters and oxidative stress defence systems.     

丹参注射液对血管紧张素Ⅱ诱导系膜细胞PAI-1表达和ROS含量的影响

目的观察丹参注射液对血管紧张素Ⅱ（AngⅡ）诱导系膜细胞纤溶酶原激活物抑制物1（PM-1）表达和活性氧（ROS）含量的影响,探讨丹参注射液抗肾小球硬化的机制。方法将培养的大鼠系膜细胞分为未干预组（N组）、AngⅡ-Ⅲ激组（A组,AnglI浓度为100nmol／L）、AngⅡ＋丹参干预组（按照丹参注射液浓度分为S1、S2和S3组,丹参注射液浓度分别为37．5g／L、75g／L和150g／L）以及单独丹参干预组（S组,丹参注射液浓度为150g／L）。S1、S2和岛组加入丹参注射液作用1h后,再加入AngⅡ（浓度为100nmol／L）共孵育24h。观察各组系膜细胞PAI-1mRNA和蛋白表达以及细胞ROS含量的变化。结果与N组相比,A组PAI-1mRNA和蛋白表达升高（P〈0．01）,S组PAI-1 mRNA和蛋白表达无差异（P〉0．05）;与A组相比,S1、S2、S3组PAI-1mRNA和蛋白表达降低（P〈0．01）。与N组相比,A组细胞R0s含量升高（P〈0．01）,S组细胞ROS含量无差异（P〉0．05）;与A组相比,S1组细胞ROS含量无差异（P〉0．05）,S2、s3组细胞ROS含量降低（P〈0．01）。结论AngⅡ通过刺激系膜细胞产生致肾纤维化因子,丹参注射液能抑制这些因子的产生。     

大鼠肝缺血及再灌注所致小肠过氧化损伤及丹参的保护作用

目的 观察大鼠肝缺血再灌注小肠过氧化损伤及丹参预处理的保护作用.方法 首先将SD大鼠随机分为正常对照组(CO组)、假手术组(SO组)、缺血再灌注组(IR组)、丹参预处理组(SM组),分别在肝缺血30、45、60 min时取上段空肠进行大体病理学检测;然后在肝缺血45 min条件下,动物亦随机分为4组(CO组、SO组、IR组、SM组),按再灌注后不同时间(0、3、12、24、72 h)分为5个亚组,每组5只.SM组在阻断第一肝门30 min前经尾静脉推注丹参注射液6 g/kg加生理盐水40 ml/kg,其余各组按40 ml/kg给予生理盐水尾静脉注入,SO组开腹后仅解剖肝门,不钳夹肝蒂.分别在再灌注0、3、12、24、72 h取上段空肠行病理学检查、丙二醛(MDA)含量测定、髓过氧化物酶(MPO)活性测定.结果 空肠黏膜损伤评分随肝缺血时限延长而加重;在肝缺血45 min再灌注不同时限点SM组空肠黏膜损伤较IR组明显减轻,且肠组织MDA含量、MPO活性均低于IR组(P<0.05).结论 肝缺血再灌注所致小肠明显淤血性损伤,MDA含量、MPO活性升高,丹参预处理对肝缺血再灌注所致小肠损伤具有保护作用.     

丹参种子特性研究△

本文研究了丹参种子不同采收部位和采收时间对种子数量和质量的影响,以及温水、高锰酸钾溶液、氯化钙溶液浸种等前处理方法对提高种子发芽率的作用。研究表明：主果穗的种子质量总体上好于侧果穗,果穗中下部的种子质量好于果穗上部,果穗上1/2~2/3果壳枯黄时进行采收才能收获的较多优质的种子。温水、高锰酸钾溶液和氯化钙溶液浸种处理对提高丹参种子发芽率都具有一定的效果,但最佳处理时间略有不同。因此,为提高收获丹参种子的质量,应尽可能在果壳半数以上至三分之二果壳枯黄时采收主果穗种子,并对果穗上端的种子进行去除。为提高陈化种子发芽率以温水浸种24 h或0.3%的高锰酸钾溶液浸种6 h处理为宜。