Summary A method is described for establishing continuous cell lines of liver epithelial cells by transformation of cultured hepatocytes isolated by collagenase/dispase of adult human liver tissue. Between 2 to 5×105 hepatocytes are inoculated into a 60 mm culture dish. The cells are incubated in a serum-free medium. Once the cells begin to divide, they are transformed by transfection with a plasmid containing SV40 early region genes. 相似文献
Simian Virus 40 large T antigen (TAg), radiolabeled with [32P]orthophosphate and with/without [3H]methionine, was found to isoelectric focus as four distinct species distributed in a highly reproducible pattern. These species have been found to differ in their ratios indicating that individual monomers of large TAg are heterogeneous in their phosphate content and suggesting, further, that large TAg may be differentially phosphorylated in vivo. Also, by increasing the concentration of large TAg the more basic species can be displaced into the more acidic species suggesting that the different isoelectric focusing forms represent aggregates of large TAg with varying phosphate content. 相似文献
The interaction of BKV with its natural target cells, human kidney epithelial cells, has not been studied. In vitro infections of human primary kidney epithelial cells were performed to investigate a BKV infection in its natural host cell. BKV undergoes a lytic replication cycle in this system: high levels of T antigen expression were first detected at 36 h postinfection, while viral DNA replication, capsid protein expression, and progeny virus were observed at 48 h postinfection. It was observed that the related polyomavirus SV40 is incapable of infecting human kidney epithelium except in the presence of the GM1 ganglioside, recently reported to be an SV40 receptor. 相似文献
To evaluate the influence of bilateral or left sympathectomy on left ventricular remodeling and function after myocardial infarction in rats.
Methods
Myocardial infarction was induced in rats by ligation of the left anterior descending coronary. Seven days later, rats were divided into 4 groups: the myocardial infarction, myocardial infarction with left sympathectomy, myocardial infarction with bilateral sympathectomy, and sham groups. After 8 weeks, left ventricular function was evaluated with the use of a pressure-volume conductance catheter under steady-state conditions and pharmacological stress. Infarct size and extracellular matrix fibrosis were evaluated, and cardiac matrix metalloproteinases and myocardial inflammatory markers were analyzed.
Results
The myocardial infarction and left sympathectomy group had an increased end diastolic volume, whereas the bilateral sympathectomy group had a mean end-diastolic volume similar to that of the sham group (P < .002). Significant reduction in ejection fraction was observed in the myocardial infarction and left sympathectomy group, whereas it was preserved after bilateral sympathectomy (P < .001). In response to dobutamine, left ventricular contractility increased in sham rats, rising stroke work, cardiac output, systolic volume, end-diastolic volume, ejection fraction, and dP/dt max. Only bilateral sympathectomy rats had significant increases in ejection fraction (P < .001) with dobutamine. Fibrotic tissue and matrix metalloproteinase expression decreased in the bilateral sympathectomy group compared to that in the myocardial infarction group (P < .001) and was associated with left ventricular wall thickness maintenance and better apoptotic markers in noninfarcted myocardium.
Conclusions
Bilateral sympathectomy effectively attenuated left ventricular remodeling and preserved systolic function after myocardial infarction induction in rats. 相似文献
Introduction: Rifamycin SV MMX®, a non-absorbable rifamycin antibiotic formulated using the multi-matrix system, was designed to exhibit its pharmacological action on the distal small intestine and colon. Its clinical efficacy and safety profile in the treatment of traveler’s diarrhea were evaluated in several clinical studies.
Areas covered: This review summarizes all available evidence regarding clinical trials of the efficacy and safety profile of rifamycin SV MMX for the treatment of traveler’s diarrhea.
Expert opinion: Rifamycin SV MMX demonstrated an excellent pharmacokinetic profile with decreased systemic toxicity similar to rifaximin. In phase II and phase III clinical trials, concerns have been raised regarding the medicine’s efficacy in terms of the time to last unformed stool and cure rate compared to current recommended antibiotics in the treatment of acute diarrhea caused by diarrheagenic Escherichia coli and invasive pathogens. The significance of the increase in MICs after the use of rifamycin SV MMX warrants further examination. 相似文献
Lupus nephritis (LN) is a highly complex autoimmune disease caused by systemic lupus erythematosus (SLE). MicroRNAs (miRNAs) play a vital role in the pathogenesis of SLE. Previously, a total of 29 miRNAs were identified to be down‐regulated in SLE patients, in which miR‐410 was likely to be involved in the signalling transduction pathways in regulating the pathogenesis of SLE. The purpose of this study was to investigate the role of miR‐410 in LN and to find out whether miR‐410 regulates the expression of interleukin (IL)‐6 and fibrosis in LN. It was found that the expression level of miR‐410 in kidney tissue of MRL/lpr mice was decreased compared to that in BALB/C mice, whereas the level of IL‐6 was overexpressed in MRL/lpr mice. Luciferase assay showed that miR‐410 binds directly to the 3′ untranslated region (UTR) of IL‐6, with the results showing that overexpression of miR‐410 significantly decreased the expression level of IL‐6 in SV40MES13 cells. Moreover, overexpression of miR‐410 significantly reduced the expression levels of fibrosis factors such as transforming growth factor‐β1 (TGF‐β1) and collagen I/III in SV40MES13 cells; Inhibition of the expression of miR‐410 with miR‐410 inhibitor resulted in increased levels of IL‐6 as well as fibrosis factors. The results identify that miR‐410, as a novel and critical factor in the pathogenesis of LN, decreases IL‐6 expression by binding directly to the 3′UTR and suppresses fibrosis through down‐regulation of TGF‐β1 in SV40MES13 cells. Our study brings new insight into understanding the complex mechanisms involved in the pathogenesis of lupus disease. 相似文献