ICP-MS法测定狼毒中12种元素

目的建立电感耦合等离子质谱(ICP-MS)法测定狼毒药材中铝、铁、锰、锌、钡、镍、硒、铅、砷、汞、镉和铜等12种微量元素的方法。方法样品微波消解后,采用电感耦合等离子质谱仪测定了12种微量元素的含量,并进行了方法学研究。结果 12种元素校正曲线线性关系良好,回收率符合痕量分析要求。结论所建立的方法准确、可靠,为狼毒药材的研究提供了依据。     

紫草中萘醌类化合物的质谱鉴别及3种成分的UPLC同时测定

目的鉴别紫草药材中的萘醌类化合物,建立紫草中3种萘醌类成分的UPLC同时测定方法,比较和分析不同产地紫草中此3种成分的含量,为建立紫草的质量评价方法提供科学依据。方法根据化合物的紫外光谱、液相色谱行为及质谱数据等综合信息鉴别紫草中的萘醌类成分,采用UPLC对紫草中紫草素、乙酰紫草素及β,β′-二甲基丙烯酰紫草素的含量进行测定,流动相为乙腈-0.1%用酸水溶液,检测波长274 nm,流速0.3mL.min-1,柱温35°C。结果对紫草药材中的5种萘醌类成分的化学归属进行了指认,并系统分析了该类成分的质谱裂解规律;测定了紫草药材中的3种紫草萘醌的含量,结果表明,紫草素、乙酰紫草素、β,β′-二甲基丙烯酰紫草素分别在0.98~98,4.40~440,3.07~307.20μg.mL-1范围内线性关系良好(R2=0.9998),平均回收率分别为(n=6)99.84%,102.23%和102.67%。结论紫草中活性成分的识别可以显著增强其质量控制的准确性和专属性,而快速准确的指标成分含量测定方法,也是质量控制的重要检测手段,定性与定量两方面结合,对于紫草药材及其相关产品的质量检测和控制具有重要意义。     

Research on fuzi based on animal thermotropism behavior to discover if it has fewer hot characteristics without Ganjiang

[OBJECTIVE]:To investigate whether Fuzi (Radix Aconiti Praeparata) has fewer hot characteristics when administered without Ganjiang (Rhizoma Zingiberis).[METHODS]:Differences in the thermotropism behaviors of mice treated either with Fuzi (Radix Aconiti Praeparata),Ganjiang (Rhizoma Zingiberis) or the combination of the two given intragastrically were investigated using the Animal Thermotropism Behavior Surveillance System.The water intake volume,oxygen consumption volume,adenosine triphosphatase (ATPase) activity,total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD) activity were determined during the investigation.[RESULTS]:When Fuzi and Ganjiang were administered together,the rate at which mice remained on a warm plate (remaining rate) and the times and distances of their movement were all significantly reduced (P＜0.05).Compared with the Normal group,the reduction was 55.1％,48.3％ and 44.8％,while compared with the Fuzi group,the reduction was 57.6％,34.3％ and 36.0％,indicating that coldtropism was significantly increased.Compared with the Normal and Fuzi groups,the ATPase activity and the respiratory oxygen consumption volume of the Fuzi + Ganjiang group were significantly increased (P＜0.05),suggesting an improvement in energy metabolism and showing a hot characteristic when Fuzi and Ganjiang are present together.Additionally,the T-AOC and T-SOD activity were significantly enhanced (P＜0.05).[CONCLUSION]:The behavior of mice tending toward cold tropism can be regarded as a quantitative reflection of Fuzi having fewer characteristics consistent with a hot nature when not used with Ganjiang,the functional mechanism of which may be a change in the ATPase activity in liver tissue.     

Pharmacological effects of Chinese herb aconite (Fuzi) on cardiovascular system

Fuzi (aconite, Radix Aconiti praeparata), a widely used Chinese herb, plays a significant role in the cardiovascular system. This is mainly reflected by Fuzi's cardiotonic effect, its protective effect on myocardial cells, and its effect on heart rate and rhythm, blood pressure, and hemodynamics. In this article, the pharmacological effects and the corresponding mechanisms of Fuzi (aconite) and its active components on cardiovascular system are reviewed.     

前列安通胶囊质量标准研究

目的制订前列安通胶囊简便、快捷的质量标准。方法在同一块薄层板上鉴别黄柏、赤芍药和丹参。采用反相高效液相法,以乙腈-甲醇-0.1%磷酸(17∶13∶70)和乙腈-甲醇-甲酸-水(10∶30∶1∶59)为流动相,分别测定了样品中盐酸小檗碱和丹酚酸B的含量。结果盐酸小檗碱进样量在0.025 2～0.252 0μg(r=0.999 999)、丹酚酸B进样量在0.108 6～1.629μg(r=0.999 99)分别与峰面积呈良好的线性关系。盐酸小檗碱与丹酚酸B平均回收率分别为99.60%、99.12%,RSD分别为0.69%、0.48%。薄层色谱图鉴别阴性无干扰。结论该质量标准可快速控制前列安通胶囊质量。     

川乌微波炮制工艺优选

目的:研究川乌的微波炮制工艺.方法:采用HPLC测定川乌不同微波炮制品中6种生物碱和总生物碱的含量,并以其为指标与传统炮制工艺进行比较,全面评价川乌微波炮制工艺.结果:最佳的微波炮制工艺为川乌经润透法处理后,于60％微波火力下炮制18 ～ 20 min,与传统炮制法比较,其总生物碱含量较高,且6种单、双型生物碱的含量均符合2010年版《中国药典》的要求.结论:该方法简单、可行而且易于控制,可作为川乌炮制的新方法.     

不同产地金樱子根、茎、果中多糖含量的比较研究

目的 通过测定4个不同产地的金樱子根、茎、果中多糖含量,初步探讨金樱子的根和茎是否能在一定程度上代替果实入药,为进一步解决金樱子药材资源匮乏问题提供科学依据。方法 采用紫外分光光度法对不同产地的金樱子中根、茎、果多糖含量进行测定。结果 4个产地金樱子根、茎的多糖含量都比果实的多糖含量低,且差异具有统计学意义。结论 金樱子根和茎不能代替果实入药。     

近十年葛根防治骨质疏松症的研究进展

葛根是一种药食同源植物,含有大量异黄酮。实验研究表明葛根及相关提取物在体内外实验中有增加骨量、改善骨代谢的功能。临床研究发现葛根能改善骨质疏松症病人的骨代谢生化指标,并改善患者的临床症状,一些防治骨质疏松症的中药复方也开始加入葛根,说明葛根治疗骨质疏松症已得到认可并应用于临床。本文对近十年来葛根防治骨质疏松症的研究进展作一综述,并对未来做出展望。     

陕西产延胡索产地加工和炮制一体化的研究

[目的]对陕西产鲜品延胡索产地加工和炮制一体化方法的研究。[方法]将鲜品延胡索分别经水煮、醋煮、醋炙和酒炙等炮制后,采用反相高效液相色谱法对各样品水提物和醇提物中延胡索乙素、去氢紫堇碱和原阿片碱进行含量测定,比较不同炮制方法对这3种药效成分的影响。[结果]延胡索经醋炙、醋煮、酒炙等炮制后,均能增加水提液、醇提液中去氢紫堇碱、延胡索乙素、原阿片碱含量。延胡索鲜品直接醋炙、醋煮、酒炙可使去氢紫堇碱、延胡索乙素含量较高。[结论]延胡索炮制工艺可采用鲜品产地加工和炮制一体化的操作方式。     

基于组合相似度丹参样本质量稳定性评价

目的:基于组合相似度进行丹参样本质量的稳定性评价。方法:采集丹参水提液的色谱指纹图谱数据,建立组合相似度,基于样本间组合相似度、共有峰夹角余弦相似度和所有峰夹角余弦相似度对9个丹参样本进行系统聚类分析,对丹参样本质量的稳定性进行评价。结果:9个丹参样本的组合相似度与所有峰夹角余弦相似度的计算值接近,2种相似度方法的系统聚类结果与丹参色谱指纹图谱的主成分分析结果一致,结果表明,9个丹参样本较好地聚为2类。结论:组合相似度可用于9个丹参样本峰比例稳定性的初步评价,为原料药质量的稳定性评价提供一种相似度方法。