侧脑室注射6-OHDA对寒冷应激诱导的大鼠血浆ACTH含量的改变

目的　观察侧脑室注射 6 OHDA对 4℃寒冷应激诱导的血浆ACTH含量的改变。方法　成年Wistar雄性大鼠 3 0只 ,随机分成 5组 (n =6) ,分别为空白对照组、寒冷应激组、6 羟基多巴胺 (6 OHDA)非应激组、6 OHDA +寒冷应激组、配药液 +寒冷应激组。所有动物处理后立即取静脉血用放射免疫法测定其血浆ACTH含量。结果　 4℃寒冷应激 4h后血浆ACTH含量明显升高 ,与正常对照组相比有明显差异 (P <0 .0 1) :6 OHDA侧脑室注射后再寒冷应激 ,血浆ACTH含量不再明显升高。结论　寒冷应激可能通过脑内儿茶酚胺能系统调控HPA轴的活性     

肿瘤标志物的联合检测在大肠癌诊断中的应用

目的探讨血清CEA、CA50、CA19-9、CA72-4、CA242等5项肿瘤标志物联合检测在大肠癌诊断中的应用价值。方法采用放免技术测定67例大肠癌2、3例大肠良性肿瘤患者和55例体检正常者血清CEA、CA50、CA19-9、CA72-4、CA242水平。结果大肠癌患者血清CEA、CA50、CA19-9、CA72-4、CA242水平及阳性率明显高于正常对照组及大肠良性肿瘤组(P<0.01)。结论CEA、CA50、CA19-9、CA72-4、CA242对大肠癌的诊断有临床意义,联合检测可提高肿瘤标志物对大肠癌的诊断准确性。     

2型糖尿病并发高血压者血清瘦素、胰岛素、胰高血糖素的水平变化及关系研究

目的：探讨2型糖尿病并发高血压者血清瘦素（Leptin）、胰岛素（Insulin）、胰高血糖素（Glucagon）水平变化与关系。方法：采用放射免疫分析对30例2型糖尿病并发高血压者和30例不伴高血压者进行血清瘦素、胰岛素及胰高血糖素含量测定,分析两组瘦素水平及其相关性。结果：两组糖尿病患者上述三指标显著高于健康正常人组（30例）,具有统计学意义（P〈0．01）;合并高血压的糖尿病病人与无高血压病人比较,差异也非常显著（P〈0．05）,且与血压的程度呈正相关关系。结论：糖尿病并发高血压者血清瘦素、胰岛素、胰高血糖素的升高,参与了本病的发生与发展过程,作为判断其病情指标有重要临床价值。     

母血、脐血INS、GLUC、COR RIA及其意义

目的：测定胎龄儿（AGA）母血、脐血胰岛素（INS）、胰高血糖素（GLUC）、皮质醇（COR）水平,探讨AGA体重与孕妇体重指数（BMI）及母血脐血INS、GLUC、COR相关性及意义。方法：随机选取无明显产科并发症的母亲及新生儿,共26对,新生儿分为体重偏低组（A组）及体重偏高组（B组）,采用放射免疫分析测定INS、GLUC、COR含量。结果：新生儿体重与孕妇BMI有明显关系,B组孕妇BMI显著高于A组（P〈0．05）;新生儿GLUC与COR水平与孕妇比较也具重要的统计学意义（P〈0．05,P〈0．01）;B组新生儿COR含量远高于A组水平（P〈0．01）,而GLUC则相反（P〈0．05）。结论：胎儿发育与孕妇BMI及母血、脐血INS、GLUC及COR水平有关,提示母亲营养状况与上述三种代谢激素的分泌水平直接影响胎儿发育。     

不同应激诱导对HELA细胞产生IL-6及leptin水平的影响

目的：研究高葡萄糖（HG）、脂多糖（LPS）及双氧水（H2O2）应激对HELA细胞上清中白细胞介素-6（IL-6）及leptin含量的影响。方法：分别给予以上三种炎症因子,建立HELA细胞炎症反应模型,采用放射免疫分析测定不同时间点细胞上清中IL-6及leptin含量的变化。结果：与空白对照组相比,三种刺激物作用6h与24h后,IL-6的含量显著升高,而leptin含量明显下降;且随着作用时间延长变化愈加明显,统计学上差异有显著性（P〈0.05）。结论：leptin可能作为一种炎性细胞因子在炎症反应中发挥一定的作用。     

120例结直肠癌手术前后血清CEA RIA测定的临床观察

为了解结直肠癌患者血清中CEA的水平与该疾病的关系 ,采用RIA法对 12 0例结直肠癌患者手术前后的血清进行了CEA检测 ,同时检测了 2 4名正常人作为对照。结果显示 :正常组CEA为 9.84± 2 .4 4ng/mL ,术前患病组CEA为 38.85± 19.2 1ng/mL ,其中结肠癌组为 37.4 3± 18.5 8ng/mL ,直肠癌组为 39.72± 2 0 .6 7ng/mL ,正常组与患病组比较P <0 .0 1,结肠癌组与直肠癌组比较P >0 .0 5。术后 2个月内对 4 4例术前CEA阳性者进行监测 ,37例CEA降至 11.2 1± 3.6 5ng/mL ,7例因手术无法根治 ,CEA为 5 0 .6 3± 2 4 .38ng/mL。术后长达 6年随访监测有 4 1例复发 ,CEA水平为 4 3.12± 2 7.15ng/mL ,4 1例复发患者中 3年内复发率占 87.80 %。结论 :CEA水平检测对结直肠癌患者术前诊断 ,术后疗效评估及监测肿瘤复发与转移是一种简便易行的有效手段。     

Effect of traditional Chinese medicine Shu-Mai-Tang on attenuating TNFalpha-induced myocardial fibrosis in myocardial ischemia rats

Shu-Mai-Tang (SMT) is a traditional Chinese medicine for treatment of ischemic heart disease. The effect of SMT on inflammation-induced myocardial fibrosis, left ventricular (LV) remodeling, and the potential mechanism in myocardial ischemia (MI) rats were investigated. Rats with ligated left anterior descending coronary artery (MI model) were randomly divided into three groups (SMTL, SMTH, and MIR). A group undergoing Sham operation (Sham; n=16) was also included. SMT (342 or 1710 mg/kg for SMTL or SMTH groups, respectively) was orally administered daily for 1 and 6 weeks. Cardiac function, myocardial fibrosis, serum tumor necrosis factor-alpha (TNFalpha) concentration, the cardiac expressions of phosphorylated p38 MAPK and tissue inhibitor of matrix metalloproteinase (TIMP)-1 and TNFalpha were examined by echocardiography, histological staining, radioimmunoassay, western blot, respectively. In the present study, significant reduced myocardial fibrosis, as well as decreased phospho-p38 MAPK, TIMP-1, and TNFalpha proteins, and serum TNFalpha level, accompanied by improved cardiac function in the SMT-treated rats in a dose-dependent manner as compared with the MIR. These results suggested that SMT could anti-inflammation-induced myocardial fibrosis and reverse LV remodeling in MI rats, and the mechanism may be related to the effect of SMT on inhibiting p38 MAPK signaling pathway.     

孕周与母儿血甲状腺激素含量的关系

目的：观察不同孕周与母儿血甲状腺激素的关系。方法：应用放射免疫分析法对67例中、晚期妊娠要求引产患者，10例早产及78例孕足月自然分娩患者进行了母血及胎儿脐静脉、脐动脉血中TSH、T3、T4的测定。结果：TSH值在母血及胎儿血中随着孕周的增加而增加．T3值在胎儿血中与母血中比较，显示低T3血症，T4值接近正常母体水平。结论：测定母血中的甲状腺激素，可动态观察不同孕周甲状腺激素变化，可及时发现胎儿低甲血症。     

Preparation and development of solid phase radioimmunoassay system for determination of cortisol

The goal of this article was oriented to prepare stable polystyrene coated tubes for direct radioimmunoassay (RIA) of human cortisol. Coating process was performed using rabbit polyclonal antisera specific for cortisol. The stability study showed that these tubes could be stored for up to one year at 4ºC without any appreciable reduction in their binding. Labeling of cortisol was carried out using chloramin-T method and the ¹²⁵I-cortisol tracer was purified using HPLC column. Preparation of polyclonal antibodies (anti-cortisol) was carried out in host rabbit animals against cortisol-21-hemisuccinate:Bovine serum albumin conjugate. The statistical analysis revealed good correlations between the results from the present study and the commercial IZOTOP RIA kit. The local may be extremely helpful in diagnosis and proper management of adrenal cortex disorders.     

Polymorphism in the C-reactive protein (CRP) gene affects CRP levels in plasma and one early marker of atherosclerosis in men: The Health 2000 Survey

The gastrointestinal hormone gastrin is measured in plasma in physiological, pathophysiological and diagnostic investigations. In the diagnosis of hypergastrinaemic diseases such as gastrinomas and gastric achlorhydria, measurement of gastrin concentrations in circulation is crucial. Gastrin circulates, however, not as a single peptide but as a mixture of peptides of different lengths and amino acid derivatizations. Moreover, in hypergastrinaemia the peptide pattern changes. Consequently, diagnostic gastrin measurements require immunoassays that recognize the pathological plasma patterns, which are characterized by a predominance of the large peptides (gastrin‐34 and gastrin‐71) and less, if any, of the shorter main form of gastrin in normal tissue, gastrin‐17. Alternatively, and in specific cases, “processing‐independent assays” (PIA) for progastrin may be considered, since hypersecreting gastrin cells also release substantial amounts of biosynthetic precursors and processing intermediates. Recently, gastrin kits that do not take the pathological plasma patterns into account have been marketed and may miss the diagnosis. Therefore, proper diagnosis of gastrinomas and other hypergastrinaemic diseases requires insight into cellular gastrin synthesis and peripheral metabolism, and also into the design of useful immunoassays. This review discusses the art of measuring gastrin in plasma with adequate diagnostic specificity.