分散固相萃取法在突发性化学物中毒检测中的应用

目的建立一种快速检测化学中毒毒物组分的新方法。方法以分散固相萃取法为前处理技术,乙酸乙酯为提取试剂,气相色谱-电子捕获检测器(GC-ECD)和气相色谱-氮磷检测器(GC-NPD)分析试样中10种毒物组分的残留。结果 10种毒物组分的加标回收率范围为72.8%～103.0%;相对标准差0.2%～8.3%;最低检出浓度0.10～0.50mg/kg。结论该方法可获得较高的回收率和精密度,较传统前处理方法更简便、快速,是一种更有效的前处理方法。     

雷公藤预处理后异体移植神经的形态学研究

[目的]研究大鼠异体坐骨神经段经雷公藤多甙(雷公藤提取物)预处理后异体移植的形态学变化。[方法]设实验组A组:异体坐骨神经段经雷公藤预处理后异体移植,B组:新鲜异体神经移植,术后口服雷公藤多甙5mg/(kg.d)5周,C组:自体右侧坐骨神经原位移植,D组:将供体直接移植于坐骨神经缺损模型。术后分时间段对移植神经片段进行形态学研究。[结果]术后2周,A、B组移植神经段早期急性排斥反应稍重于C组,但小于D组;至8周时A、B组移植段的炎性细胞浸润程度明显减轻,与C组相似,而D组炎性细胞浸润明显;术后14周时A、B、C3组移植神经段外膜构成完整,束膜、内膜厚度,分布及范围无明显差异,再生大量神经纤维,髓鞘完整,排列规则,再生有髓神经神经纤维的面积,总数和髓鞘厚度差异无统计学意义(P﹥0.05),而D组基本未见神经纤维再生。[结论]异体神经段经雷公藤多甙预处理后可降低免疫排斥反应,促进周围神经再生。     

Ⅲ/Ⅳ期子宫内膜异位症不孕患者预处理对辅助生殖技术结果的影响

目的:分析预处理对子宫内膜异位症(EMs)不孕患者辅助生殖技术(ART)结果的影响。方法:回顾性分析238例行ART的Ⅲ/Ⅳ期EMs不孕患者的妊娠结局,观察未行预处理及两种不同预处理后的ART结局。结果:手术组、GnRHa预处理组及未处理组间行IVF超促排卵周期窦卵泡数目、Gn的剂量、用药时间、HCG日血清E2水平、内膜厚度、获卵数、成熟卵泡数、胚胎数、D1时2PN胚胎数、D3时1级胚胎数、妊娠率、累积妊娠率比较均无统计学差异(P>0.05)。结论:IVF前手术及GnRHa预处理并不能提高IVF成功率,若无明显手术指征,拟行辅助生殖技术之前行腹腔镜手术或者GnRHa治疗(间隔>3月)只会增加患者经济负担。     

Effect of Pretreatment with Lactobacillus delbrueckii and Streptococcus thermophillus on Tailored Triple Therapy for Helicobacter pylori Eradication: A Prospective Randomized Controlled Clinical Trial

Background: Helicobacter pylori plays an important role in gastric cancer and typical eradication regimensare no longer effective in many countries, including Thailand. The aim of our study was to compare the effectof Lactobacillus delbrueckii and Streptococcus thermophillus on tailored triple therapy for Helicobacter pylorieradication. Materials and Methods: This prospective single-center study was conducted in Thailand. Helicobacterpylori associated gastritis patients were randomized to 2 groups: group 1 (n=100) was tailored triple therapywith placebo (esomeprazole 20 mg bid, clarithromycin 500 mg bid or metronidazole 400 mg tid if clarithromycinresistance and amoxicillin 1000 mg bid), and group 2 was tailored triple therapy plus pretreatment with probioticcontaining yogurt. Successful eradication was defined as both negative histology and negative rapid urease testat four weeks after treatment. Results: A total of 200 infected patients were enrolled. PP analysis involved 194patients: 96 in the tailored triple therapy with placebo group (group 1) and 98 the in tailored triple therapyplus pretreatment with probiotic containing yogurt group (group 2). Successful eradication was observed in170 (87.6%) patients; by PP analysis, the eradication rate was significantly higher in group 2 (P = 0.04, 95%CI;0.02-0.13) than in group 1. ITT analysis also showed that the value was significantly higher in the tailored triplethreapy plus pretreatment with probiotic containing yogurt group (group 2) (89/100; 89%) than in the tailoredtriple therapy with placebo group (group 1) (P= 0.01, 95%CI; 0.04-0.15). In terms of adverse events, there wasno significant difference between the two groups. Conclusions: Pretreatment with probiotic containing yogurtcan improve Helicobacter pylori eradication rates with tailored triple therapy. Adding probiotics does not reduceadverse effects of the medication.     

西妥昔单抗联合化疗治疗晚期肿瘤患者的护理

<正>西妥昔单抗是表皮生长因子(EGFR)的人/鼠嵌和的单克隆抗体,EGFR在多种表皮组织中表达,西妥昔单抗与正常或肿瘤细胞EGFR特异性结合,且竞争性抑制EGFR和其它配体结合,以阻止受体     

老年卵巢癌的治疗策略

老年卵巢癌是指年龄大于65岁女性的卵巢癌。近半数(45%)的患者在诊断时大于64岁。随着人口老龄化和预期寿命的提高,老年卵巢癌患者的比例将会进一步增加,由于治疗并发症和毒副反应,老年患者多未接受标准治疗(满意的肿瘤细胞减灭术和以铂类为基础的联合化疗),因此老年卵巢癌的生存率没有提高甚至出现下降。对大部分老年卵巢癌患者实施标准治疗是可行的。为了提供最佳的治疗方案,治疗前的评估是必要的。另外,还需对老年卵巢癌患者进行前瞻性研究,以期为老年卵巢癌患者是否能从治疗中获益提供证据。     

预先心理应激对干舱模拟快速上浮脱险大鼠应激反应的作用

目的 观察预先心理应激对大鼠在模拟快速上浮脱险过程中应激反应的影响.方法 40只健康雄性SD大按数字随机表法鼠随机等分为4组:对照组、心理应激组、快速加减压组、预先心理应激+快速加减压组.通过建立快速上浮脱险动物模型,观察预先心理应激对该过程中大鼠应激反应的作用.结果 预先进行心理应激的动物在快速上浮脱险过程中,心率明显加快,血清肿瘤坏死因子(TNF-α)表达明显降低.结论 心理应激在模拟快速上浮脱险大鼠应激反应中起重要作用.

Abstract：

Objective To observe the effects of psychological pretreatment on the stress reaction of simulated fast buoyancy ascent escape in rats. Methods Forty healthy rats were randomly divided into 4 groups:the control group, the psychological stress group, the fast compression and decompression group, and the psychological stress pretreatment plus fast compression and decompression group. By developing the fast buoyancy ascent escape rat model with fast compression and decompression in a closed cabin, the effects of psychological pretreatment on the stress reaction were observed in the course of ascent. Results Hear t rate of the animals in the psychological pretreatment stress group was significantly higher than that in the control group (P＜0. 05), and the expression of serum TNF-α was significantly lower than that in the control group (P ＜ 0.05 ). Conclusions Psychological factor seemed to have positive effects on the stress reaction of the simulated fast buoyancy ascent escape in rats.     

Comparison of Dosimetric Performance among Commercial Quality Assurance Systems for Verifying Pretreatment Plans of Stereotactic Body Radiotherapy Using Flattening-Filter-Free Beams

The purpose of this study was to compare the performance of different commercial quality assurance (QA) systems for the pretreatment verification plan of stereotactic body radiotherapy (SBRT) with volumetric arc therapy (VMAT) technique using a flattening-filter-free beam. The verification for 20 pretreatment cancer patients (seven lung, six spine, and seven prostate cancers) were tested using three QA systems (EBT3 film, I’mRT MatriXX array, and MapCHECK). All the SBRT-VMAT plans were optimized in the Eclipse (version 11.0.34) treatment planning system (TPS) using the Acuros XB dose calculation algorithm and were delivered to the Varian TrueBeam^® accelerator equipped with a high-definition multileaf collimator. Gamma agreement evaluation was analyzed with the criteria of 2% dose difference and 2 mm distance to agreement (2%/2 mm) or 3%/3 mm. The highest passing rate (99.1% for 3%/3 mm) was observed on the MapCHECK system while the lowest passing rate was obtained on the film. The pretreatment verification results depend on the QA systems, treatment sites, and delivery beam energies. However, the delivery QA results for all QA systems based on the TPS calculation showed a good agreement of more than 90% for both the criteria. It is concluded that the three 2D QA systems have sufficient potential for pretreatment verification of the SBRT-VMAT plan.     

制备黄豆花生染色体标本前处理的初步研究

在染色体标本制备中,对黄豆离体根尖用0.002M8-羟基喹啉、0.05％秋水仙碱及前二者的等量混合液分别在18℃恒温下前处理三小时,冷蒸馏水(4℃～5℃)中前处理22小时,以8-羟基喹啉在18℃处理出现的中期分裂相最多。用0.002M8-羟基喹啉在18℃～20℃恒温下,对黄豆和花生根尖进行不同间隔时间前处理,结果观察到黄豆根尖的晚前期和中期分裂相以3～3.5小时最多;花生根尖的中期分裂相以2～4小时最多。