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排序方式: 共有591条查询结果,搜索用时 15 毫秒
51.
目的:研究黑加仑多糖对免疫功能低下小鼠的免疫调节作用。方法:给小鼠腹腔注射环磷酰胺,建立小鼠免疫功能低下模型后,以0.4,0.8和1.6g/kg的黑加仑多糖灌胃,连续10d,另设模型对照组和正常对照组。观察免疫低下小鼠的脾、胸腺指数变化情况;用分光光度法测定小鼠巨噬细胞吞噬数、抗体分泌细胞生成数、血清溶血素和IL-2等免疫指标的含量。结果:黑加仑多糖高、中、低剂量组对免疫功能低下小鼠的脾、胸腺指数有增强作用,IL-2产生增加,增强血清溶血素的功能(P0.05),其中高、中剂量组对巨噬细胞吞噬功能有增强作用(P0.05)。黑加仑多糖高剂量组对免疫功能低下小鼠抗体分泌细胞功能有增强作用(P0.05)。结论:黑加仑多糖具有增强免疫功能的作用。 相似文献
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商陆多糖Ⅱ体外对小鼠脾细胞增殖及产生集落刺激因子的影响 总被引:10,自引:0,他引:10
用[3H]TdR参入法检测小鼠脾细胞增殖能力及产生集落刺激因子(colony stimulating factor. CSF)含量.证明商陆多糖Ⅱ(PAP-Ⅱ)在31~500 μg·m-1范围内显著促进小鼠稗细胞增殖。PAP-Ⅱ,31~125 μg·ml-1可剂量依赖性地促进Con A(1,2.8μg·ml-1),LPS(3,10,30 μg·ml-1)诱导的淋巴细胞细咆增殖,随着PAP-Ⅱ剂量加大,对丝裂原诱导的淋巴细胞增殖反呈抑制作用。PAP-Ⅱ。10~500μg·ml-1呈剂量及时间依赖性地促进脾细胞产生CSF,其最适剂量为100 μg·ml-1。最佳时间为5 d,提示PAP-Ⅱ能增强免疫及促进造血功能。 相似文献
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目的研究翡翠贻贝多糖对流感病毒在狗肾细胞(MDCK)中增殖的抑制作用。方法采用血凝滴度测定研究翡翠贻贝多糖对MDCK细胞培养甲型流感病毒的抑制作用;通过联合用药试验研究翡翠贻贝多糖对利巴韦林抗流感病毒的协同效应。结果翡翠贻贝多糖能显著降低MDCK细胞培养流感病毒的血凝滴度;翡翠贻贝多糖与利巴韦林联合使用时,能显著抑制MDCK细胞培养流感病毒的增殖并具有相加效应。结论翡翠贻贝多糖能明显抑制MDCK细胞培养流感病毒的增殖,对利巴韦林抗流感病毒具有相加效应,具有开发成抗流感病毒生物制剂的前景。 相似文献
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目的:探讨牛膝多糖(AbP)及其硫酸酯和磷酸酯衍生物对糖尿病(DM)大鼠胰岛细胞形态和肾功能的影响。方法:以链脲佐菌素诱导SD大鼠致DM模型,观察AbP、牛膝多糖硫酸酯衍生物(S-AbP)和牛膝多糖磷酸酯衍生物(P-AbP)对DM大鼠血糖、胰岛细胞形态和分泌的影响;测定血清肌酐(Scr)和尿素氮(BUN)水平,观察其对DM大鼠肾功能的影响。结果:AbP及其两种衍生物均可明显降低DM大鼠的血糖浓度(P〈0.05或P〈0.01),S-AbP和P-AbP可明显减轻DM大鼠胰岛β细胞损伤,使胰岛素阳性反应细胞增多;S-AbP显著降低Scr和BUN水平(均P〈0.01),P-AbP和AbP显著降低Scr水平(均P〈0.01)。结论:S-AbP和P-AbP对高血糖所致胰岛β细胞损伤有明显的保护和修复作用,对肾功能有不同程度的改善作用。 相似文献
59.
Staphylococcus epidermidis is now well established as a major nosocomial pathogen, associated with indwelling medical devices. Its major virulence factor is related with the ability to adhere to indwelling medical devices and form biofilms. In this study, the biofilm matrix of four S. epidermidis clinical isolates was extracted and the polysaccharides and proteins content was quantified. The results were correlated with the total biofilm biomass (determined by crystal violet assay) and cellular metabolic activity (evaluated with XTT reduction assay). According to the results, the exopolymers studied play an important role not only on structure and biofilm biomass but also on cellular activity. Thus, the strain forming biofilms with the highest level of polysaccharides (S. epidermidis 1457) also formed thicker biofilms but with the lowest metabolic activity. The protein concentration also varied among strains, with the biofilm matrix of S. epidermidis 9142 presenting a higher concentration of proteins comparing to the remaining strains. This fact indicates the different levels of importance that matrix proteins can hold on biofilm composition among strains albeit overall, it is suggested that extracellular protein production it is not a determinative factor for biofilm total biomass. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献
60.
To the valorization of Indocalamus tessellatus(I.tessellatus) leaves for the functional food industry, the process of ultrasonic-assisted deep eutectic solvent (DES) extraction of I.tessellatus leaves polysaccharides (U-DES-ICP) was optimized by response surface methodology. In comparison to ultrasonic extracted polysaccharides (UICP) and water extracted polysaccharides (WICP), their physicochemical properties were analyzed by HPLC, HPGPC-MALLS, and FTIR, and the structure morphology was observed by SEM. Their antioxidant activities were evaluated based on DPPH and ABTS + assay as well. The results showed that the best DES system for U-DES-ICP was a mixture of choline chloride and malonic acid in the molar ratio of 1:4 (10% water content). The highest yield of U-DES-ICP (0.6812%) was obtained by following optimized conditions: 60 min of sonication time, 17 mL/g of liquid-solid ratio, 500W of sonication power, and 63.5 °C of sonication temperature, which was significantly higher than that of the conventional methods. The structural analysis indicated that U-DES-ICP had more uronic acid with higher molecular weight compared with UICP and WICP, the relative content of uronic acid of U-DES-ICP reached 8.364%, which was twice as much as the other two polysaccharides; its molecular weight reached up to 6.201 × 103 KD, and the highest values of molecular weight of UICP and WICP were 1.684 × 103 KD and 995.4 KD, U-DES-ICP which were confirmed by SEM. The antioxidant assays reflected that U-DES-ICP possessed stronger scavenging capacities both on DPPH and ABTS + radicals with IC50 (0.48 mg/mL and 0.61 mg/mL) than the other two biomacromolecules. IC50s for DPPH and ABTS radical scavenging by UICP were 0.51 and 0.73 mg/mL, while 0.82 and 0.94 mg/mL were IC50s for both radical scavenging by WICP, respectively. Therefore, this study evidenced that a novel DES system could boost the extraction of I.tessellatus leaves polysaccharides, which produced a higher yield of product with stronger biological activities compared with traditional techniques. 相似文献