肺结核病人PPD皮试与ELISPOT结果的相关性分析

[目的]研究肺结核病人酶联免疫斑点技术(ELISPOT)和结核菌素纯蛋白衍生物(PPD)皮试结果的相关性.[方法]24例痰结核菌培养阳性肺结核病人,38例临床疑似肺结核病人,取外周血分离获取单个核细胞(PBMCs)后行ELISPOT试验.[结果]痰结核菌培养阳性肺结核组与临床疑似肺结核组PPD皮试阳性率相似;痰结核菌培养阳性肺结核组的ELISPOT阳性率高于临床疑似肺结核组;ELISPOT斑点数与PPD皮试反应程度之间缺乏相关性.[结论]ELISPOT的诊断特异性高于PPD皮试.     

血清结核抗体检测和PPD试验在结核病诊断中的应用

目的 对运用结核抗体检测以及PPD试验在结核诊断中的价值进行评估.方法 对我站收治的200例肺结核患者以及100例排除结核的其他疾患者的血清内结核抗体进行检测,同时对每组患者进行PPD试验.结果 采用结核抗体检测结核病患者有166例出现阳性反应,PPD试验中有170例出现阳性,两组方法在阳性检出率方面比较,差异无统计学...     

天津市汉沽区儿童卡介苗免疫接种效果分析

目的了解天津市汉沽辖区近3年内结核菌素儿童卡介苗接种的免疫效果。方法对4 281名已初种卡介苗儿童结核菌素（PPD）试验结果进行分析。结果 3个月～3岁、4～5岁和6～7岁3个年龄组PPD试验阳性率,2008、2009和2010年分析为97.3%～85.7%、97.1%～83.3%和97.2%～90.0%。结论汉沽近3年儿童卡介苗接种免疫效果较好,接种质量较高。抓好新生儿卡介苗初种质量,是提高卡介苗接种成功率的关键。     

The role of non-specific lymphocytes in antigen-induced proliferative response in vitro

The antigen-induced proliferative response of lymph node cells from immunized mice is proportional to the number of primed lymphocytes in the microtiter wells. Addition of normal lymphocytes did not alter the magnitude of the antigen-specific [³H]TdR uptake of immune lymph node cells. In contrast, normal lymphocytes increased the antigen-specific thymidine incorporation of enriched populations of antigen-specific lymphocytes. This enhancing effect was especially pronounced, and proportional to the number of supplementing unsensitized lymphocytes, with a small cell number of enriched lymphocytes, where the specific responsiveness could barely be detected without addition of normal lymphocytes.Enriched populations derived through adherence to antigen-pulsed macrophages (‘selected’ cultures) as well as those obtained by growing immune lymph node cells with antigen-pulsed macrophages (‘supernatant’ cultures) had an improved proliferative response after addition of normal lymphocytes. However, the proliferative response of the ‘selected’ cultures was extremely dependent on normal lymphocytes as they could express only 10% of their full stimulatory capability in the absence of the latter. This indicates that the blastogenic signal delivered by the activated specific T cells recruits normal lymphocytes which do not adhere to the antigen-pulsed macrophages. Under the same conditions the recruiting signal is incapable of inducing the multiplication of antigen-sensitized cells.The potential recruitable uncommitted lymphocytes are present in excess among immune lymph node cells, while depleted from the ‘selected’ cultures. The enriched ‘supernatant’ cultures seem to contain considerable numbers of recruitable lymphocytes although they are present in quantities less than are required to provide for the full amplifying signal.This meaning of these findings for the evaluation of enrichment of antigen-specific T cells and of antigen-induced response based on [³H]TdR uptake is discussed.     

活动性肺结核与PPD反应相关性观察

目的探讨活动性肺结核病人在不同情况下的PPD反应强度。方法分析361例活动性肺结核在不同类型、年龄、病变范围、传染性、性别的PPD反应强度。结果活动性肺结核的PPD反应强度与患病类型、年龄有关、而与病变范围、传染性、患者的性别无关。结论PPD实验材料简单,创伤小,强阳性率高,患者顺应性好,是临床活动性肺结核简而易便的有效辅诊技术。     

胸水抗PPD-IgG在结核性胸膜炎中的临床意义

目的：评价胸水抗PPD－IgG检测对结核性胸膜炎的临床意义。方法：采用斑点免疫金渗滤技术（金标法）检测70例结核性胸膜炎患血清及胸水抗PPD－IgG，同时作PPD皮试，并随机选择24例非结核性胸腔积液作为对照组。结果：结核组血清、胸水中抗PPD－IgG阳性中分别为60．00％和52．86％，显高于对照组血清及胸水中抗PPD－IgG阳性中（8．33％和16．67％），P＜0．01；血清抗PPD－IgG敏感性为60．00％，特异性91．67％，胸水抗PPD－IgG敏感性52．86％，特异性83．33％，同时测胸水、血清抗PPD-IgG敏感性68．57％，特异性91．67％。结核组PPD总阳性率73．81％。结论：同时测定血清、胸水抗PPD－IgG联合PPD皮试，将会提高结核性胸膜炎诊断的敏感性和诊断率。     

结核菌H37Ra免疫小鼠后PPD刺激淋巴细胞增殖转化的探讨

目的:探讨小鼠接种H37Ra后,PPD刺激鼠淋巴细胞的增殖转化情况.方法:用H37Ra皮内、腹腔免疫小鼠后30、60天,检测小鼠脾淋巴细胞受PPD刺激后的转化率.结果:小鼠接种H37Ra后,30天淋转率分别为21.98%(腹腔)和24.85%(皮内);60天淋转率分别为25.50%(腹腔)和25.02%(皮内),明显高于未免疫组(P＜0.01);与BCG皮内免疫组比较,无显著性差异(P＞0.05);H37Ra腹腔和皮内不同途径接种,淋巴细胞转化率无明显差异.结论:H37Ra免疫小鼠后能诱导机体产生抗结核的免疫应答.     

20(R)-原人参二醇酸水解条件的工艺研究

目的:优化三七茎叶总皂苷的盐酸水解工艺,提高水解产物20(R)-原人参二醇[20(R)-protopanaxadiol,20(R)-PPD]的产率。方法:采用正交试验设计,考察料液比、盐酸浓度、水解时间、水解温度对三七茎叶总皂苷水解过程的影响。结果:影响水解的主次因素依次是盐酸浓度>料液比>水解温度>水解时间,优选的最佳工艺为料液比1∶1;盐酸浓度17.1mol·L-1,水解时间90min,水解温度50℃。结论:采用优化后的水解条件能够提高PPD的转化率,适合工业化生产。