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71.
Osteogenic evaluation of glutaraldehyde crosslinked gelatin composite with fetal rat calvarial culture model 总被引:1,自引:0,他引:1
The cytotoxicity of the synthetic bone substitute composed of tricalcium phosphate and glutaraldehyde crosslinked gelatin (GTG) were evaluated by osteoblast cell culture. In a previous study, the GTG composites were soaked in distilled water for 1, 2, 4, 7, 14, 28, and 42 days, and then the solutions (or extracts) were cocultured with osteoblasts to evaluate the cytotoxicity of GTG composites by alive cell counting. In this study, the extracts were cocultured with the osteoblasts; thereafter, the concentration of transforming growth factor-beta (TGF-beta1) and prostaglandin E2 (PGE2) in the medium was analyzed to strictly reflect the biological effects of GTG composites on the growth of osteoblasts. In order to investigate the osteoconductive potential of the GTG composites on new bone formation in a relative short term, a model of neonatal rat calvarial organ culture was designed prior to animal experiments. Three experimental materials of 4, 8, and 12% GTG composites were evaluated by fetal rat calvarial organ culture for their ability for bone regeneration. Deproteinized bovine and porcine cancellous bone matrixes were used as the controlled materials. All the organ culture units were maintained in cultured medium for 5 weeks. Following the culture period, the morphology of tissue was observed under an optical microscope, and the quantitative evaluation of the new generation bone was determined by using a semiautomatic histomorphometeric method. Except in the initial 4 days, the concentration of TGF-beta1 of 4% and 8% GTG composites was higher than that of the blank group for all the other experimental time periods. The PGE2 concentration for 4% and 8% GTG composites was lower than that of the blank group. It revealed that the 4% and 8% GTG composites would not lead to inflammation and would promote osteoblast growth. The morphology and activity of the osteoblasts were not transformed or changed by the 2 GTG composites. For the 12% GTG composite, the performance of the in vitro condition was inferior to the blank group and the other 2 GTG composites. Although the concentration of TGF-beta1 and PGE2 was gradually back to normal after 14 days, the morphology of the osteoblasts was abnormal with features such as contracted cytoplast structures. The osteoblast was damaged perhaps in the initial stage. We suggested that the 4% and 8% GTG composites should be soaked in distilled water at least for 4 days before medical applications. The 12% GTG composite and the composites with a concentration of glutaraldehyde solution higher than 12% were not recommended as a medical prostheses in any condition. The fetal rat calvaria culture also showed the same results with the analysis of TGF-beta1 and PGE2. From the study, we could predict the results of animal experiments in the future. 相似文献
72.
黄芪多糖的提取及对体外培养成骨细胞成骨能力的影响 总被引:30,自引:0,他引:30
目的:了解黄芪多糖对体外培养成骨细胞增殖、分化能力的影响。方法:采用水煮醇沉法提取黄芪多糖,用TLC进行定性,用比色法定量,用含高低两种剂量(0.5m g/m l,5.0m g/m l)的黄芪多糖的培养液体外培养成骨细胞,MTT法及ALP比活性测定观察成骨细胞增殖率及细胞活性变化。结果:低浓度黄芪多糖(0.5m g/m l)及高浓度黄芪多糖(5.0m g/m l)短期时(2 天)促进成骨细胞增殖;高浓度(5.0m g/m l)长期(4 天)抑制成骨细胞增殖,降低其活性。结论:黄芪多糖对成骨细胞增殖、活性有双向调节作用 相似文献
73.
74.
外力诱导培养兔成骨细胞表达c—fos的实验研究 总被引:2,自引:1,他引:1
目的:探讨兔成骨细胞在机械力作用下将机械力信号转变为生物学效应的作用机理;方法:利用FOS免疫组化技术观察在受到外力后培养的兔成骨细胞对立即早期基因c-fos的蛋白产物(FOS)的表达情况;结果:几乎所有的成骨细胞核均染成黄褐色,为FOS免疫组化反应阳性;结论:c-fos在兔成骨细胞的机械力信号向生物学效应转换的过程中可能发挥重要作用。 相似文献
75.
Calcitonin has direct effects on3[H]-thymidine incorporation and alkaline phosphatase activity in human osteoblast-line cells 总被引:8,自引:0,他引:8
John R. Farley Jon E. Wergedal Susan L. Hall Sandra Herring Nanine M. Tarbaux 《Calcified tissue international》1991,48(5):297-301
Summary Calcitonin had direct and dose-dependent actions on human osteoblast-line cells (in serum-free monolayer culture) to increase
cell proliferation and alkaline phosphatase activity/mg cell protein. Salmon calcitonin increased (human osteosarcoma) SaOS-2
cell proliferation, as evidenced by dose-dependent increases in3[H]-thymidine incorporation into DNA (e.g., 153% of control after 20 h exposure at 0.1 nM,P<0.01), and MTT (thyzolyl blue) reduction/deposition (e.g., 161% of control after 72 h exposure at 0.03 nM). Continuous exposure
was not required to elicit these proliferative responses. These effects were not unique to salmon calcitonin or to SaOS-2
cells. Similar effects were seen with human calcitonin (but not heat-inactivated human calcitonin) and with (human osteosarcoma)
TE-85 cells and human osteoblast-line cells prepared from femoral heads. In addition to effects on cell proliferation, calcitonin
also increased alkaline phosphatase-specific activity in SaOS-2 cells (e.g., 180% of control after 72 h of exposure to 0.1
nM salmon calcitonin,P<.005). 相似文献
76.
D. D. Bikle 《Osteoporosis international》2008,19(9):1237-1246
Bone loss during skeletal unloading, whether due to neurotrauma resulting in paralysis or prolonged immobilization due to
a variety of medical illnesses, accelerates bone loss. In this review the evidence that skeletal unloading leads to bone loss,
at least in part, due to disrupted insulin like growth factor (IGF) signaling, resulting in reduced osteoblast proliferation
and differentiation, will be examined. The mechanism underlying this disruption in IGF signaling appears to involve integrins,
the expression of which is reduced during skeletal unloading. Integrins play an important, albeit not well defined, role in
facilitating signaling not only by IGF but also by other growth factors. However, the interaction between selected integrins
such as αυβ3 and β1 integrins and the IGF receptor are of especial importance with respect to the ability of bone to respond
to mechanical load. Disruption of this interaction blocks IGF signaling and results in bone loss.
The skeletal response to mechanical load is critical for maintenance of skeletal integrity. This review will assess the interacting
roles that insulin like growth factor I (IGF-I) signaling and selected integrins play in this response. Skeletal unloading
results in decreased integrin expression, resistance to the anabolic actions of IGF-I, and bone loss. 相似文献
77.
Takayuki Sakai Yukio Okano Yoshinori Nozawa Nobumitsu Oka 《Journal of bone and mineral metabolism》1991,9(1):19-25
The effects of parathyroid hormone (rat 1–34 fragment, rPTH) on phosphoinositide metabolism and intracellular Ca2+ mobilization were studied in a osteoblast-like clone MC3T3-E1 cells. rPTH caused a transient rise in intracellular Ca2+ in a dose-dependent manner. Significant Ca2+ increase was still observed under the extracellular Ca2+-free condition. In addition, 100nM rPTH stimulated rapid formation of both 1,2-diacylglycerol (1,2-DAG) and inositol 1,4,5-trisphosphate
(Ins(1,4,5)P3), indicating agonist-triggered hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2).
The current observations suggested that the rPTH-induced increase of intracellular Ca2+ was in part due to internal Ca2+ release mediated by Ins(1,4,5)P3. These results indicate possible involvement of phosphoiniositide metabolism in signal transduction of PTH-stimulated osteoblastic
cells. 相似文献
78.
Expression of bone sialoprotein (BSP) in developing human tissues 总被引:14,自引:0,他引:14
Paolo Bianco Larry W. Fisher Marian F. Young John D. Termine Pamela Gehron Robey 《Calcified tissue international》1991,49(6):421-426
Summary Bone sialoprotein (BSP) and its messenger RNA were localized in developing human skeletal and nonskeletal tissues by means
of immunohistochemistry andin situ hybridization. Both protein and mRNA were found in mature, bone-forming cells but not in their immature precursors. In addition,
osteoclasts displayed positive immunostaining and high densities of autoradiographic grains byin situ hybridization experiments. BSP was expressed in fetal epiphyseal cartilage cells, particularly in hypertrophic chondrocytes
of growth plates. Though neither the protein nor the mRNA were identified in a variety of other connective and nonconnective
tissues, an unexpected finding was the expression of BSP in the trophoblast cells of placenta. These findings show that BSP
is primarily an osteoblast-derived component of the bone matrix expressed at late stages of differentiation. We have also
found that osteoclasts produce BSP, possibly as a mediator of cell attachment to bone. 相似文献
79.
目的:了解异黄酮类药Genistein对体外培养成骨细胞的作用。方法:应用MTT法、对硝基苯磷酸盐法,原子吸收分光光度法及茜素红染色方法观察Genistein对体外培养成骨细胞的增殖、ALP表达,基质钙含量及矿化结节形成的影响。结果:Genistein具有刺激成骨细胞增殖,提高ALP活性,细胞基质钙含量及矿化结节形成的数量的作用。结论:Genistein具有刺激体外培养成骨细胞增殖,分化成熟及促进矿化的作用。 相似文献
80.
右归饮对体外成骨细胞增殖和分化影响的实验研究 总被引:7,自引:0,他引:7
目的在右归饮治疗激素性股骨头坏死的临床研究和动物实验研究基础上,进一步观察对体外成骨细胞增殖和分化的影响.方法采用血清药理学方法,对体外培养的大鼠成骨细胞作MTT法细胞增殖测定、PNPP法ALP活性测定、以及茜素红染色矿化结节计数.结果培养48、72h,10%和15%右归饮血清组的细胞增殖速度较对照组快(p<0.05);培养72h,10%和15%右归饮血清组的ALP活性较对照组增加(p<0.05);培养2w,矿化结节计数显示15%右归饮血清组多于对照组(p<0.01).结论右归饮对体外大鼠成骨细胞的增殖和分化具有促进作用. 相似文献