芦笋多糖提取、单糖组分分析及定量测定

目的提取芦笋多糖,纯化后分析其单糖组分并测定总糖,为今后芦笋多糖研究提供参考。方法水提醇沉法提取粗多糖,Sevage法除蛋白,H2O2脱色,高效毛细管电泳(HPCE)测定单糖组成,苯酚-硫酸法测定多糖。结果芦笋粗多糖主要单糖组成为木糖、果糖、鼠李糖、阿拉伯糖、半乳糖,其质量分数分别为3.44%、7.92%、10.52%、17.15%、41.85%。以单糖混合物为对照品测得粗多糖中总糖的质量分数为79.14%。结论高效毛细管电泳分离度高,可用于单糖组分分析;苯酚-硫酸法实验操作简便,准确可靠,可用于芦笋多糖的测定。     

Anti-inflammatory effects of YeongyoSeungma-tang

Ethnopharmacological relevance

YeongyoSeungma-tang which includes Fructus Forsythia, has been used in oriental traditional medicine for treatment of early smallpox and atopic dermatitis.

Aim of the study

YeongyoSeungma-tang was carried out to investigate for anti-inflammatory effects.

Materials and methods

YeongyoSeungma-tang was tested for anti-inflammatory effects against lipopolysaccharide (LPS)-induced nitric oxide (NO), prostaglandin E₂ (PGE₂), and tumor necrosis factor alpha (TNF-α) releases as well as nuclear factor kappa B (NF-κB) expression using RAW264.7 macrophage cells.

Results

YeongyoSeungma-tang significantly inhibited generation of NO (42% and 59% inhibition at doses of 5 μg/mL and 10 μg/mL, respectively), PGE₂ (46% and 80% inhibition at doses of 5 μg/mL and 10 μg/mL, respectively) and TNF-α (6% and 23% inhibition at doses of 5 μg/mL and 10 μg/mL, respectively) on LPS-stimulated RAW 264.7 cells in a concentration-dependent manner. Consistently in these observations, the expression of inducible NO synthase (iNOS) enzyme was also inhibited by YeongyoSeungma-tang. However, YeongyoSeungma-tang did not show any influence on the expression of cyclooxygenase-2. The cream containing 0.075% YeongyoSeungma-tang showed good skin moisturizing effect without any irritation.

Conclusion

The present study may support the fact that YeongyoSeungma-tang can have the good possibility as an anti-inflammatory agent for troubled skins.     

拉毛果叶化学成分的研究

 目的 研究拉毛果叶子的化学成分。方法 采用硅胶柱及Sephadax LH-20凝胶柱色谱法分离化合物,运用现代光谱技术鉴定化合物结构。结果 从拉毛果叶中分离得到6个化合物,分别鉴定为：β-胡萝卜苷(Ⅰ)、肥皂草苷(Ⅱ)、芹菜素-7-O-β-D-葡萄糖苷(Ⅲ)、异牡荆素(Ⅳ)、异荭草素(Ⅴ)、木犀草素-7-O-β-D-葡萄糖苷(Ⅵ)。结论 化合物Ⅰ～Ⅳ均为首次从该植物中分离得到,其中化合物Ⅱ～Ⅵ为首次从川续断属植物中分离得到。     

中国宽叶缬草药用功效研究概况

从宽叶缬草的镇静活性、抗抑郁活性、调节血脂及抗脂质过氧化作用、对肾脏的保护作用、以及对胆囊结石和心、脑血管系统疾病的治疗作用等七个方面综述了近三年来国内对宽叶缬草药用功效的研究进展。     

番荔枝种子的化学成分研究

目的研究番荔枝Annona squamosa种子的化学成分。方法利用色谱分离纯化,根据化合物的理化性质和波谱数据进行结构鉴定。结果分离鉴定了26个化合物,其中10个为双四氢呋喃环型番荔枝内酯类化合物,分别为泡番荔枝辛(1)、番荔辛(2)、去乙酰紫玉盘素(3)、去乙酰异紫玉盘素(4)、大花紫玉盘素A(5)、motrilin(6)、番荔枝塔亭丁(7)、番荔枝塔亭戊(8)、番荔枝塔亭甲(9)、12,15-顺式番荔枝塔亭甲(10)。结论化合物4和10为首次从番荔枝种子中分离得到。     

不同来源罗勒挥发油成分的GC-MS法分析及体外抗菌活性

应用GC-MS法分析来源于三个不同产地的唇形科罗勒属植物罗勒Ocimum basilicum Linn.的全草挥发油成分,并以金黄色葡萄球菌、大肠杆菌和白色念珠菌作为鉴定菌,测试不同来源罗勒油的体外抗菌活性.结果表明:蒿脑是其主要的共有组分,但不同来源罗勒挥发油的成分组成差异较大,其抗菌活性也表现不一.     

Anti-anxiety activity of Coriandrum sativum assessed using different experimental anxiety models

Interest in alternative medicine and plant-derived medications that affect the "mind" is growing. The aim of present study was to explore the anti-anxiety activity of hydroalcoholic extract of Coriandrum sativum (Linn.) using different animal models (elevated plus maze, open field test, light and dark test and social interaction test) of anxiety in mice. Diazepam (0.5 mg/kg) was used as the standard and dose of hydroalcoholic extract of C. sativum fruit (50, 100 and 200 mg/kg) was selected as per OECD guidelines. Results suggested that extract of C. sativum at 100 and 200 mg/kg dose produced anti-anxiety effects almost similar to diazepam, and at 50 mg/kg dose did not produce anti-anxiety activity on any of the paradigm used. Further studies are needed to identify the anxiolytic mechanism(s) and the phytoconstituents responsible for the observed central effects of the hydroalcoholic extract of C. sativum.     

国产西洋参各部位中微量元素的测定

作者用荧光、极谱和原子吸收光谱法检测出国产西洋参各部位中都含有人体必需常量元素钾、钙、镁、钠和微量元素铁、锌、铜、锰、钴、硒(Ⅳ)、镍、钼,而有毒微量元素铅、镉的含量则较低。所测15种微量元素和其中8种人体必需微量元素的加和量均值,植株地上部分(茎杆、叶、种子)皆高于地下部分(须根、主根、根茎);过去不曾入药的须根、根茎和叶其含量远高于主根。除主根外,其余各部位中微量元素的含量,在国内外是首次单独检测的。     

叶下珠种质资源遗传多样性的ISSR分析

目的对分布于我国30个居群的叶下珠进行遗传多样性分析。方法采用ISSR分子标记技术,研究来自我国重庆、陕西、河南、云南、广西、广东、浙江、贵州、福建、海南省区共30个叶下珠居群的遗传多样性。结果从60个ISSR引物中筛选出24个用于扩增,共扩增得到264条条带,其中共有条带19条,多样性条带245条,多态位点百分率(PPB)为92.80%,遗传相似性系数(GS)值在0.579 5～0.916 7,多态性较高。UPGMA聚类结果显示我国叶下珠居群大致可以分为3支,即内陆分支、沿海分支及位于云南省区的过渡分支;个别居群呈现明显的居群特异性。结论叶下珠种质资源遗传多样性较高。