圆滑番荔枝的化学成分及生物活性研究进展

圆滑番荔枝为番荔枝属植物,民间常用作杀虫药和驱虫剂.从圆滑番荔枝分离到的化学成分主要有番荔枝内酯类、萜类、环肽、生物碱等,其中番荔枝内酯类化合物占大多数.圆滑番荔枝具有抗肿瘤、杀虫、抗菌、阻碍孢子萌发等多种生物活性,应用前景广阔.为更好地挖掘圆滑番荔枝的药用价值及进一步开发利用,现对其化学成分和生物活性研究进展进行综述,以提供参考.     

不同产地掌叶大黄HPLC指纹图谱的比较

目的 应用RP—HPLC(DAD)对不同产地掌叶大黄药材进行指纹图谱比较。方法 用Inertsil ODS—3分析柱，1％HAc—H20与1％HAc—CH3CN梯度洗脱，流速1mL／min，柱温30℃，检测波长280nm，参比波长380nm。结果 建立了HPLC指纹图谱共有模式，并对其他产地药材进行了相似度比较。结论 对大黄药材中各成分均得到很好分离，可作为大黄药材的具有专属性的指纹图谱。     

Two New Germacranolides from Magnolia Grandiflora

Abstract

Two new sesquiterpenoids, 4,5-epoxy-13-methoxy-1(10)-germacren-12,6-olide and 4,5-epoxy-13-acetoxy-1(10)-germacren-12,6-olide, were isolated from the leaves of Magnolia grandiflora, together with six known compounds, 2α-hydroxy-dihydroparthenolide, parthenolide, costunolide, syringaresinol, (+) medioresinol and 6,7-dimethoxycoumarin. The structures of the new compounds were elucidated on the basis of spectroscopic methods and X-ray diffraction.     

藏药喜马拉雅紫茉莉的本草考证及种属问题探讨

目的:通过对藏药喜马拉雅紫茉莉的本草考证及其与紫茉莉异同点的对比研究,明确喜马拉雅紫茉莉的基原,解决喜马拉雅紫茉莉的种属问题,达到正本清源的目的。方法:对历代本草著作进行考证,结合现代文献加以分析,并比较喜马拉雅紫茉莉与同科植物紫茉莉的异同点。结果:从本草考证来看,喜马拉雅紫茉莉的用药古今一致,应归类为山紫茉莉属植物。讨论:喜马拉雅紫茉莉与紫茉莉的用药部位(根)性状相似,但性味功效区别较大,不得混用。     

Comparative study of chemical composition and antioxidant activity of fresh and dry rhizomes of turmeric (Curcuma longa Linn.)

The phytoconstituents of essential oil and ethanol oleoresin of fresh and dry rhizomes of turmeric (Curcuma longa Linn.) were analyzed by GC–MS. The major constituents were aromatic-turmerone (24.4%), alpha-turmerone (20.5%) and beta-turmerone (11.1%) in fresh rhizome and aromatic-turmerone (21.4%), alpha-santalene (7.2%) and aromatic-curcumene (6.6%) in dry rhizome oil. Whereas, in oleoresins, the major components were alpha-turmerone (53.4%), beta-turmerone (18.1%) and aromatic-turmerone (6.2%) in fresh and aromatic-turmerone (9.6%), alpha-santalene (7.8%) and alpha-turmerone (6.5%) in dry rhizome. Results showed that alpha-turmerone, a major component in fresh rhizomes is only minor one in dry rhizomes. Also, the content of beta-turmerone in dry rhizomes is less than a half amount found in fresh rhizomes. The antioxidant properties have been assessed by various lipid peroxidation assays as well as DPPH radical scavenging and metal chelating methods. The essential oil and ethanol oleoresin of fresh rhizomes have higher antioxidant properties as compared dry ones.     

Chrysanthemum indicum linnéextracts attenuate the mitogenic effect of epinephrine on human HCC cells

Objective: To investigate the stimulatory effect of epinephrine(Epi) and the antagonistic effect of Chrysanthemum indicum Linné extracts (CILE) on Epi-induced growth of human hepatocellular carcinoma(HCC) cells. Methods: The stimulatory effect of Epi and inhibitory effect of CILE on the growth of HepG2 and MHCC97H cells were investigated using a proliferation assay in correlation with βadrenergic receptor(β 2-AR) blockade, a MEK1/MEK2 inhibitor, and assessment of MAPK/ERK1/2 intracellular activity. Results:...     

越南安息香中1个新的苯丙素类化合物

目的 研究越南安息香（白花树Styrax tonkinensis的干燥树脂）的化学成分及其细胞毒活性。方法 采用MCI gel CHP-20P、硅胶、Sephadex LH-20柱色谱、快速制备色谱和制备HPLC等技术进行分离和纯化,通过IR、1D-和2D-NMR、HR-ESI-MS等谱学数据分析确定化合物的结构。通过CCK-8法评价了化合物的细胞毒性。结果 从越南安息香提取物中分离得到6个化合物,分别鉴定为（E）-3-[3-甲氧基-4-（3-乙氧基-2-氧代乙氧基）苯基]烯丙基苯甲酸酯（1）、2-（4-羟基-3-甲氧基苯基）-5-（3-羟丙基）-7-甲氧基苯并呋喃（2）、（＋）-雪松素（3）、（＋）-松脂素（4）、松脂素单甲醚（5）和安息香脂素（6）。以上6个化合物对人宫颈癌HeLa细胞株、人肝癌HepG-2细胞株、人乳腺癌MCF细胞株、人前列腺癌PC-3细胞株以及人肺癌A549细胞株的半数抑制浓度值均大于100 μmol/L。结论 化合物1为未见报道的新苯丙素类化合物,命名为白花树酯醚,化合物2～6为首次从越南安息香中分离得到。化合物1～6均未表现出明显的细胞毒活性。     

Evaluation of anti-inflammatory and anti-arthritic property of ethanolic extract of Clitoria ternatea

Objective: Clitoria ternatea is a well-known bioactive plant used to treat several inflammatory ailments in Ayurvedic system of medicine in India. The present investigation aimed to determine the anti-inflammatory and anti-arthritic activity of ethanolic extract of Clitoria ternatea roots (EECT) in animal models. Methods: The anti-inflammatory activity of the EECT was evaluated by carrageenan and histamine-induced paw edema. Results: EECT showed a significant reduction in mean paw edema volume in both carrageenan and histamine-induced inflammation. The efficacy of EECT in rheumatoid arthritis was tested against Freund’s complete adjuvant (CFA) induced arthritic models in Wistar rats. The anti-arthritic effect of EECT was determined by systematic scoring of arthritis symptoms and measuring paw edema. A considerable decrease in paw diameter was observed in the EECT (200 and 400 mg/kg) and diclofenac (10 mg/kg) treated groups after day 7. Diclofenac (10 mg/kg) and EECT (400 mg/kg) showed a significant reduction in paw diameter from day 14 compared with CFA control (P < 0.001). The anti-arthritic activity was also confirmed from the altered biochemical, haematological (Hb, RBC and WBC) and anti-oxidant parameters (SOD, MDA, CAT, and GSH). EECT (400 and 200 mg/kg) also showed a marked inhibition of joint destruction. Conclusion: This study provides a pharmacological rationale for the traditional use of C. ternatea against inflammation and rheumatoid arthritis in India.     

HPLC法测定苎麻根中大黄素的含量

目的建立苎麻根中大黄素的含量测定方法。方法采用VP-ODS C18（4.6 mm×250 mm,5μm）色谱柱。以甲醇-0.1%磷酸溶液（80∶20）为流动相;流速为1.0 ml·min-1;检测波长为254 nm。结果苎麻根中的大黄素含量在0.1014~0.5070μg范围内进样量与峰面积的线性关系良好（r=0.9999）;平均回收率为100.11%,RSD为1.11%。结论所建立的方法准确、灵敏、重复性好,可用于苎麻根的质量控制。     

萝芙木属植物药理活性和单萜吲哚生物碱合成途径研究进展

萝芙木属植物中的蛇根木是印度传统草药,目前萝芙木属植物成为中国常用南药,主要用于治疗高血压、精神类疾病、心血管疾病、癌症等。对国产萝芙木属植物资源种类及分布、药理活性、萝芙木单萜吲哚生物碱的合成途径等研究进展进行综述,为萝芙木属植物的研究和临床应用提供参考。